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Bypassing Iron Storage In Endodermal Vacuoles Rescues The Iron Mobilization Defect In The Natural Resistance Associated-Macrophage Protein3natural Resistance Associated-Macrophage Protein4 Double Mutant, Viviane Mary, Magali Schnell Ramos, Cynthia Gillet, Amanda L. Socha, Jerome Giraudat, Astrid Agorio, Sylvain Merlot, Colin Clairet, Sun A. Kim, Tracy Punshon, Mary Lou Guerinot, Sebastien Thomine
Bypassing Iron Storage In Endodermal Vacuoles Rescues The Iron Mobilization Defect In The Natural Resistance Associated-Macrophage Protein3natural Resistance Associated-Macrophage Protein4 Double Mutant, Viviane Mary, Magali Schnell Ramos, Cynthia Gillet, Amanda L. Socha, Jerome Giraudat, Astrid Agorio, Sylvain Merlot, Colin Clairet, Sun A. Kim, Tracy Punshon, Mary Lou Guerinot, Sebastien Thomine
Dartmouth Scholarship
To improve seed iron (Fe) content and bioavailability, it is crucial to decipher the mechanisms that control Fe storage during seed development. In Arabidopsis (Arabidopsis thaliana) seeds, most Fe is concentrated in insoluble precipitates, with phytate in the vacuoles of cells surrounding the vasculature of the embryo. NATURAL RESISTANCE ASSOCIATED-MACROPHAGE PROTEIN3 (AtNRAMP3) and AtNRAMP4 function redundantly in Fe retrieval from vacuoles during germination. When germinated under Fe-deficient conditions, development of the nramp3nramp4 double mutant is arrested as a consequence of impaired Fe mobilization. To identify novel genes involved in seed Fe homeostasis, we screened an …
Myb10 And Myb72 Are Required For Growth Under Iron-Limiting Conditions, Christine M. Palmer, Maria N. Hindt, Holger Schmidt, Stephan Clemens, Mary Lou Guerinot
Myb10 And Myb72 Are Required For Growth Under Iron-Limiting Conditions, Christine M. Palmer, Maria N. Hindt, Holger Schmidt, Stephan Clemens, Mary Lou Guerinot
Dartmouth Scholarship
Iron is essential for photosynthesis and is often a limiting nutrient for plant productivity. Plants respond to conditions of iron deficiency by increasing transcript abundance of key genes involved in iron homeostasis, but only a few regulators of these genes have been identified. Using genome-wide expression analysis, we searched for transcription factors that are induced within 24 hours after transferring plants to iron-deficient growth conditions. Out of nearly 100 transcription factors shown to be up-regulated, we identified MYB10 and MYB72 as the most highly induced transcription factors. Here, we show that MYB10 and MYB72 are functionally redundant and are required …
Arabidopsis Bhlh100 And Bhlh101 Control Iron Homeostasis Via A Fit-Independent Pathway, Alicia B. Sivitz, Victor Hermand, Catherine Curie, Grégory Vert
Arabidopsis Bhlh100 And Bhlh101 Control Iron Homeostasis Via A Fit-Independent Pathway, Alicia B. Sivitz, Victor Hermand, Catherine Curie, Grégory Vert
Dartmouth Scholarship
Iron deficiency induces a complex set of responses in plants, including developmental and physiological changes, to increase iron uptake from soil. In Arabidopsis, many transporters involved in the absorption and distribution of iron have been identified over the past decade. However, little is known about the signaling pathways and networks driving the various responses to low iron. Only the basic helix–loop–helix (bHLH) transcription factor FIT has been shown to control the expression of the root iron uptake machinery genes FRO2 and IRT1. Here, we characterize the biological role of two other iron-regulated transcription factors, bHLH100 and bHLH101, in iron homeostasis. …
Disruption Of Osysl15 Leads To Iron Inefficiency In Rice Plants, Sichul Lee, Jeff C. Chiecko, Sun A. Kim, Elsbeth L. Walker, Youngsook Lee, Mary Lou Guerinot, Gyhheung An
Disruption Of Osysl15 Leads To Iron Inefficiency In Rice Plants, Sichul Lee, Jeff C. Chiecko, Sun A. Kim, Elsbeth L. Walker, Youngsook Lee, Mary Lou Guerinot, Gyhheung An
Dartmouth Scholarship
Uptake and translocation of metal nutrients are essential processes for plant growth. Graminaceous species release phytosiderophores that bind to Fe3+; these complexes are then transported across the plasma membrane. We have characterized OsYSL15, one of the rice (Oryza sativa) YS1-like (YSL) genes that are strongly induced by iron (Fe) deficiency. The OsYSL15 promoter fusion to β-glucuronidase showed that it was expressed in all root tissues when Fe was limited. In low-Fe leaves, the promoter became active in all tissues except epidermal cells. This activity was also detected in flowers and seeds. The OsYSL15:green …
Chloroplast Fe(Iii) Chelate Reductase Activity Is Essential For Seedling Viability Under Iron Limiting Conditions, Jeeyon Jeong, Christopher Cohu, Loubna Kerkeb, Marinus Pilon, Erin L. Connolly, Mary Lou Guerinot
Chloroplast Fe(Iii) Chelate Reductase Activity Is Essential For Seedling Viability Under Iron Limiting Conditions, Jeeyon Jeong, Christopher Cohu, Loubna Kerkeb, Marinus Pilon, Erin L. Connolly, Mary Lou Guerinot
Dartmouth Scholarship
Photosynthesis, heme biosynthesis, and Fe-S cluster assembly all take place in the chloroplast, and all require iron. Reduction of iron via a membrane-bound Fe(III) chelate reductase is required before iron transport across membranes in a variety of systems, but to date there has been no definitive genetic proof that chloroplasts have such a reduction system. Here we report that one of the eight members of the Arabidopsis ferric reductase oxidase (FRO) family, FRO7, localizes to the chloroplast. Chloroplasts prepared from fro7 loss-of-function mutants have 75% less Fe(III) chelate reductase activity and contain 33% less iron per microgram of chlorophyll than …
A Novel Iron-Regulated Metal Transporter From Plants Identified By Functional Expression In Yeast., David Eide, Margaret Broderius, Jeanette Fett, Mary Lou Guerinot
A Novel Iron-Regulated Metal Transporter From Plants Identified By Functional Expression In Yeast., David Eide, Margaret Broderius, Jeanette Fett, Mary Lou Guerinot
Dartmouth Scholarship
Iron is an essential nutrient for virtually all organisms. The IRT1 (iron-regulated transporter) gene of the plant Arabidopsis thaliana, encoding a probable Fe(II) transporter, was cloned by functional expression in a yeast strain defective for iron uptake. Yeast expressing IRT1 possess a novel Fe(II) uptake activity that is strongly inhibited by Cd. IRT1 is predicted to be an integral membrane protein with a metal-binding domain. Data base comparisons and Southern blot analysis indicated that IRT1 is a member of a gene family in Arabidopsis. Related sequences were also found in the genomes of rice, yeast, nematodes, and humans. In Arabidopsis, …