Digital Commons Network^™

Bacterial Community Profiling Of The Eastern Oyster (Crassostrea Virginica): Comparison Of Culture-Dependent And Culture-Independent Outcomes, Kenneth J. La Valley, Steve Jones, Marta Gomez-Chiarri, Joseph Dealteris, Michael A. Rice

Marta Gomez-Chiarri

Tissue-associated bacterial community profiles generated using a nested polymerase chain reaction–denaturing gradient gel electrophoresis (DGGE) approach and culture-dependent and culture-independent isolation techniques were compared. Oyster samples were collected from 2 harvest areas along the coast of Maine, in the United States. Profiles from both isolation strategies were evaluated using Sorensen’s index of similarity and cluster analysis of gel banding patterns. Cultureindependent profiles were further evaluated using the Shannon diversity index. In general, the culture-dependent strategy resulted in a greater number of bands within a profile. BacterialDGGEprofiles were found to be highly similar within an isolation strategy, with a higher degree …

Seaweed Cultivation Pilot Trials – Towards Culture Systems And Marketable Products, Pia C. Winberg, Danielle Skropeta, Alex Ullrich

Dr Pia C Winberg

Globally, seaweed is the largest aquaculture production by volume at over eight million wet metric tonnes per annum (FAO 2003). Mostly this production is for traditional foods in Asia and the commodity markets of agar, alginates and carageenans. However, there is also untapped potential in smaller, high product value markets for nutritional and health applications. This is where Australia's best investment in a seaweed industry may lie. Australia has a number of advantages and opportunities that present themselves with regard to the development of a seaweed cultivation industry. Of particular advantage for Australia is the large coastal zone area with …

Farm Chemicals As Indicators Of Sediment Sources In Iowa Rivers, George P. Malanson, E. N. Nealson

George P Malanson

Determination of the source of sediment in rivers and streams is important in order to effectively implement a program to reduce its concentration. This project uses agricultural chemicals as indicators of current sources of sediment from farm fields in the Cedar River, Iowa watershed. We hypothesized that the relations of sediment, nitrogen, and phosphorous yields to precipitation would indicate whether sediment originated from erosion of fields or from channel bank erosion of floodplains. The changes in sediment, nitrate, and phosphorus in the channel in response to rainfall events were determined. In simple regressions, all three variables have similar slopes when …

Habitat And Plant Distributions In Hanging Gardens Of The Narrows, Zion National Park, Utah, George P. Malanson

George P Malanson

No abstract provided.

Marine Park Planning And Recreational Fishing: Is The Science Lost At Sea? Case Studies From Australia, Daryl Mcphee

Daryl McPhee

“No take” marine parks are widely advocated and increasingly utilised as a tool aimed at protecting marine biodiversity, and they may also be used as a fisheries management tool. In Australia a national system of no-take marine parks is being established to protect marine biodiversity. The creation of this network of no-take marine parks that exclude the public from recreational fishing in large areas of the ocean is extremely contentious. Nearly one quarter of the Australian population goes recreational fishing at least once a year, and it is a $2 billion industry. The motivations for, and aspirations of these recreational …

Seaweed Cultivation Pilot Trials – Towards Culture Systems And Marketable Products, Pia C. Winberg, Danielle Skropeta, Alex Ullrich

Danielle Skropeta

Globally, seaweed is the largest aquaculture production by volume at over eight million wet metric tonnes per annum (FAO 2003). Mostly this production is for traditional foods in Asia and the commodity markets of agar, alginates and carageenans. However, there is also untapped potential in smaller, high product value markets for nutritional and health applications. This is where Australia's best investment in a seaweed industry may lie. Australia has a number of advantages and opportunities that present themselves with regard to the development of a seaweed cultivation industry. Of particular advantage for Australia is the large coastal zone area with …

Accounting For False Positive Detection Error Induced By Transient Individuals, Chris Sutherland, D Elston, X. Lambin

Chris Sutherland

Environmental, Human, And Socioeconomic Characteristics Of Pedestrian Injury And Death In Las Vegas, Nv, Courtney Coughenour, Jennifer Pharr, Tim Bungum

Courtney Coughenour

No abstract provided.

Teasaponin Reduces Inflammation And Central Leptin Resistance In Diet-Induced Obese Male Mice, Yinghua Yu, Yizhen Wu, Alexander Szabo, Zhixiang Wu, Hongqin Wang, Duo Li, Xu-Feng Huang

Yizhen Wu

Chronic inflammation is involved in the pathogenesis of obesity and type 2 diabetes. Recently, teasaponin, an extract from tea, has been shown to have anti-inflammatory effects. We examined the effect of teasaponin on obesity, inflammation, glucose metabolism and central leptin sensitivity, in obese mice fed a high-fat (HF) diet for 16 weeks. Intraperitoneal injections of teasaponin (10mg/kg, daily) for 21 days significantly decreased the food intake and body weight of HF diet-induced obese mice. Teasaponin treatment also reduced the protein levels of pro-inflammatory cytokines (TNF-α, IL-6 and/or IL-1β) and NF-κB signaling (p-IKK and p-IκBα) in adipose tissue and the liver. …

Teasaponin Reduces Inflammation And Central Leptin Resistance In Diet-Induced Obese Male Mice, Yinghua Yu, Yizhen Wu, Alexander Szabo, Zhixiang Wu, Hongqin Wang, Duo Li, Xu-Feng Huang

Yizhen Wu

Chronic inflammation is involved in the pathogenesis of obesity and type 2 diabetes. Recently, teasaponin, an extract from tea, has been shown to have anti-inflammatory effects. We examined the effect of teasaponin on obesity, inflammation, glucose metabolism and central leptin sensitivity, in obese mice fed a high-fat (HF) diet for 16 weeks. Intraperitoneal injections of teasaponin (10mg/kg, daily) for 21 days significantly decreased the food intake and body weight of HF diet-induced obese mice. Teasaponin treatment also reduced the protein levels of pro-inflammatory cytokines (TNF-α, IL-6 and/or IL-1β) and NF-κB signaling (p-IKK and p-IκBα) in adipose tissue and the liver. …

A Sense Of Home: A Cultural Geography Of The Leschenault Estuary, Sandra Wooltorton

Sandra Wooltorton

Spatial–Temporal Dynamics Of Stable Fly (Diptera: Muscidae) Trap Catches In Eastern Nebraska, David B. Taylor, Kristina Friesen, Jerry J. Zhu

David B. Taylor

Spatial and temporal relationships among catches of adult stable flies, Stomoxys calcitrans (L.), on sticky traps in eastern Nebraska were evaluated. Twenty-five alsynite sticky traps were placed in a 5 by 5 grid with 1.6-km intervals in a mixed agricultural environment from 2003 to 2011. Denser grids of 45-90 traps were implemented for varying lengths of time during the course of the study. More than two million stable flies were collected over 9 yr. Seasonal abundances based upon total collections from the primary grid of 25 traps were bimodal most years with population peaks in June and September or October. …

Activities Of Methionine-Γ-Lyase In The Acidophilic Archaeon “Ferroplasma Acidarmanus” Strain Fer1, M. A. Khan, Madeline M. Lopez-Munoz, Charles W. Kaspar, Kai F. Hung

Kai F. Hung

Biogeochemical processes on exposed pyrite ores result in extremely high levels of sulfuric acid at these locations. Acidophiles that thrive in these conditions must overcome significant challenges, including an environment with proton concentrations at pH 3 or below. The role of sulfur metabolism in the archaeon “Ferroplasma acidarmanus” strain fer1’s ability to thrive in this environment was investigated due to its growth-dependent production of methanethiol, a volatile organic sulfur compound. Two putative sequences for methionine- γ-lyase (EC 4.4.1.11), an enzyme known to carry out α,γ-elimination on L-methionine to produce methanethiol, were identified in fer1. Bioinformatic analyses identified a conserved pyridoxal-5′-phosphate …

Bacterial Community Profiling Of The Eastern Oyster (Crassostrea Virginica): Comparison Of Culture-Dependent And Culture-Independent Outcomes, Kenneth J. La Valley, Steve Jones, Marta Gomez-Chiarri, Joseph Dealteris, Michael A. Rice

Michael A Rice

Tissue-associated bacterial community profiles generated using a nested polymerase chain reaction–denaturing gradient gel electrophoresis (DGGE) approach and culture-dependent and culture-independent isolation techniques were compared. Oyster samples were collected from 2 harvest areas along the coast of Maine, in the United States. Profiles from both isolation strategies were evaluated using Sorensen’s index of similarity and cluster analysis of gel banding patterns. Cultureindependent profiles were further evaluated using the Shannon diversity index. In general, the culture-dependent strategy resulted in a greater number of bands within a profile. BacterialDGGEprofiles were found to be highly similar within an isolation strategy, with a higher degree …

Refeeding After Acute Food Restriction: Differential Reduction In Preference For Ethanol And Ethanol-Paired Flavors In Selectively Bred Rats., Nancy K. Dess, C. D. Chapman, L. A. Cousins, D. C. Monroe, P. Nguyen

Nancy K Dess

Rats' voluntary ethanol intake varies with dispositional factors and energy status. The joint influences of these were of interest here. We previously reported that rats selectively bred for high voluntary saccharin intake (HiS) consume more ethanol and express more robust conditioning of preference for flavors paired with voluntarily consumed ethanol than do low-saccharin consuming counterparts (LoS). Three new experiments examined the effect of refeeding after an episode of food restriction on ethanol intake and on preference for ethanol-paired flavors in HiS and LoS rats. A 48-h episode of food restriction with wheel running reduced intake of and preference for 4% …

Assessing Multiple Endpoints Of Atrazine Ingestion On Gravid Northern Watersnakes (Nerodia Sipedon) And Their Offspring, Lorin A. Neuman-Lee, Karen F. Gaines, Kyle A. Baumgartner, Jayme R. Voorhees, James M. Novak, Stephen J. Mullin

Karen F. Gaines

Ecotoxicological studies that focus on a single endpoint might not accurately and completely represent the true ecological effects of a contaminant. Exposure to atrazine, a widely used herbicide, disrupts endocrine function and sexual development in amphibians, but studies involving live-bearing reptiles are lacking. This study tracks several effects of atrazine ingestion from female Northern Watersnakes (Nerodia sipedon) to their offspring exposed in utero. Twenty-five gravid N. sipedon were fed fish dosed with one of the four levels of atrazine (0, 2, 20, or 200 ppb) twice weekly for the entirety of their gestation period. Endpoints for the mothers included blood …

Real And Perceived Damage By Wild Turkeys: A Literature Review, Scott R. Groepper, Scott E. Hygnstrom Dr, Brandon Houck, Stephen M. Vantassel

Scott R Groepper

As populations of wild turkeys (Meleagris gallopavo) have increased, the number of complaints about damage has increased. We conducted a literature review to determine real and perceived damage caused by wild turkeys in North America. Wild turkeys can cause damage to agricultural crops, such as corn (Zea mays L.), soybeans (Glycine max [L.] Merrill), wheat, and hay crops but the majority of actual damage is usually minor or caused by other wildlife, thus estimates of damage by wild turkeys often are inflated. Occasionally, wild turkeys damage specialty crops, turfgrass, or ornamental flowers that may have higher value than common agricultural …

The Role Of Alcohol In Forging And Maintaining Friendships Amongst Scottish Men In Mid-Life, Carol Emslie, Kate Hunt, Antonia Lyons

Dr Carol Emslie

Objective: Men drink more heavily and are more likely to die from alcohol-related causes than women. Most alcohol research focuses on young drinkers. We describe the context of men’s drinking in mid-life and explore how alcohol is associated with the construction of masculinities.

Methods: Qualitative research was used to examine the social context of drinking alcohol. We conducted 15 focus groups (single and mixed sex) with respondents in the west of Scotland, UK. Here, we focus on the findings from 22 men aged 28 to 52 years.

Results: Men regarded drinking pints of beer in the pub together as an …

Divergence In Thyroid Hormone Concentrations Between Juveniles Of Marine And Stream Ecotypes Of The Threespine Stickleback (Gasterosteus Aculeatus), Jun Kitano, Sean C. Lema

Sean Lema

Background: Hormones regulate the expression of multiple phenotypic traits. Therefore,

divergence in hormone concentrations may lead to evolutionary changes in the coordinated

physiological and behavioural traits that comprise an organism’s integrated phenotype. Adults

of marine ecotypes of threespine stickleback (Gasterosteus aculeatus) have higher concentrations

of the thyroid hormone thyroxine (T4) than adults of stream-resident ecotypes (Kitano

et al., 2010). Thyroid hormones are well-established mediators of osmoregulation and migratory

behaviours in fish, and the difference in T4 concentrations indicates that changes in thyroid

hormone signalling may underlie the evolutionary and ecological divergence of migratory and

non-migratory ecotypes.

Questions: Is the variation …

Hormones And Phenotypic Plasticity: Implications For The Evolution Of Integrated Adaptive Phenotypes, Sean C. Lema, Jun Kitano

Sean Lema

It is generally accepted that taxa exhibit genetic variation in phenotypic plasticity, but many questions remain unanswered about how divergent plastic responses evolve under dissimilar ecological conditions. Hormones are signaling molecules that act as proximate mediators of phenotype expression by regulating a variety of cellular, physiological, and behavioral responses. Hormones not only change cellular and physiological states but also influence gene expression directly or indirectly, thereby linking environmental conditions to phenotypic development. Studying how hormonal pathways respond to environmental variation and how those responses differ between individuals, populations, and species can expand our understanding of the evolution of phenotypic plasticity. …

Effects Of Flow Restoration On Mussel Growth In A Wild And Scenic North American River, Brandon J. Sansom, Daniel J. Hornbach, Mark C. Hove, Jason S. Kilgore

Daniel J. Hornbach

No abstract provided.

Cph601 Chapter 3 Risk Assessment, David M. Mannino

David M. Mannino

No abstract provided.

Enhancing Theutility Of Visitor Impact Assessment In Parks And Protected Areas: A Combined Social-‐‐ Ecological Approach, A. D'Antonio, Christopher Monz, P. Newman, S. Lawson, D. Taff

Christopher Monz

No abstract provided.

Modeling Simultaneous Selection For Resistance And Tolerance In Goldenrod (Solidago Altissima) Across A Range Of Spittlebug Population Densities, Michael J. Wise, Warren G. Abrahamson Ii

Warren G. Abrahamson, II

Plant defenses against herbivory include two main strategies: resistance (to minimize the amount of damage) and tolerance (to minimize the fitness impact of that damage). Recent studies have emphasized the need to consider both strategies simultaneously for a fuller understanding of the evolutionary and ecological dynamics of plant defense against herbivores. We used a combination of a garden study, a greenhouse experiment, and mathematical modeling to investigate resistance to and tolerance of spittlebug damage in the goldenrod Solidago altissima. In contrast to traditional expectations, the genetic correlation between resistance and tolerance was highly positive. Selection gradients indicated that directional selection …

Arsenic Exposure And Oral Cavity Lesions In Bangladesh, Emdadul H. Syed, Krishna C. Poudel, Junko Yasuka, Keiko Otsuka, Alauddin Ahmed, Tariqul Islam, Farque Pavez, Vesna Slavkovich, Joseph H. Graziano, Habibul Ahsan, Masamine Jimba

Krishna C. Poudel

Objective—To evaluate the relationship between arsenic exposure and oral cavity lesions among an arsenic-exposed population in Bangladesh. Methods—We carried out an analysis utilizing the baseline data of the Health Effects of Arsenic Exposure Longitudinal Study (HEALS). HEALS is an ongoing population-based cohort study to investigate health outcomes associated with arsenic exposure via drinking water in Araihazar, Bangladesh. We used multinomial regression models to estimate the risk of oral cavity lesions. Results—Participants with high urinary arsenic levels (286.1–5000.0μg/g) were more likely to develop arsenical lesions of the gums [multinomial odds ratio (M-OR 2.90; 95% CI= 1.11–7.54)], and tongue (M-OR 2.79; 95% …

The Effect Of Resource Stress On Goldenrod's Tolerance Of Folivory Depends More On The Identity Of The Stress Than On The Severity Of The Stress, Peter J. March, Michael J. Wise, Warren G. Abrahamson Ii

Warren G. Abrahamson, II

It is widely accepted that the levels of resources in a plant’s environment can influence the plant’s ability to compensate for (i.e., tolerate) damage by herbivores. However, predicting the direction of the influence has proven difficult. Here, we report on a greenhouse study in which individuals of Solidago altissima were exposed to factorial combinations of light and fertilization levels to investigate how different types of stresses affect plants’ ability to tolerate leaf damage by larvae of the beetle Trirhabda virgata. Shade stress reduced the plants’ tolerance of herbivory, while nutrient stress had no effect on tolerance. These results did not …

Health Care And Hiv Testing Experiences Among Black Men In The South: Implications For "Seek, Test, Treat And Retain" Hiv Prevention Strategies, Rupali K. Doshi, David J. Malebranche, Lisa Bowleg, Thurka Sangaramoorthy

David J Malebranche

Few studies have explored how overall general health care and HIV/STI testing experiences may influence receipt of ‘‘Seek, Test, Treat, and Retain’’ (STTR) HIV prevention approaches among Black men in the southern United States. Using in-depth qualitative interviews with 78 HIV-negative/unknown Black men in Georgia, we explored factors influencing their general health care and HIV/STI testing experiences. The Andersen behavioral model of health care utilization (Andersen model) offers a useful framework through which to examine the general health care experiences and HIV testing practices of Black men. It has four primary domains: Environment, Population characteristics, Health behavior, and Outcomes. Within …