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Design Of A Low-Cost Capillary Electrophoresis Laser-Induced Fluorescence System: Lessons Learned When Trying To Build The Lowest Possible Cost System, Steven James Perry
Design Of A Low-Cost Capillary Electrophoresis Laser-Induced Fluorescence System: Lessons Learned When Trying To Build The Lowest Possible Cost System, Steven James Perry
Theses and Dissertations
Capillary electrophoresis laser-induced fluorescence (CE-LIF) is widely used to detect both the presence and concentration of fluorescently labeled biomolecules. In CE-LIF, a plug of sample fluid is electrophoretically driven down a microchannel using a high voltage applied between the opposite ends of the microchannel. Molecules of different sizes and charge states travel at different velocities down the channel. Laser light with a wavelength in the excitation band of the fluorophores is focused near the end of the channel. As each species of molecule passes through the laser spot, the fluorophores emit a fluorescence signal which is measured with an optical …
Design Of A Low-Cost Capillary Electrophoresis Laser-Induced Fluorescence System: Lessons Learned When Trying To Build The Lowest Possible Cost System, Steven James Perry
Design Of A Low-Cost Capillary Electrophoresis Laser-Induced Fluorescence System: Lessons Learned When Trying To Build The Lowest Possible Cost System, Steven James Perry
Theses and Dissertations
Capillary electrophoresis laser-induced fluorescence (CE-LIF) is widely used to detect both the presence and concentration of fluorescently labeled biomolecules. In CE-LIF, a plug of sample fluid is electrophoretically driven down a microchannel using a high voltage applied between the opposite ends of the microchannel. Molecules of different sizes and charge states travel at different velocities down the channel. Laser light with a wavelength in the excitation band of the fluorophores is focused near the end of the channel. As each species of molecule passes through the laser spot, the fluorophores emit a fluorescence signal which is measured with an optical …