Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Immunoblotting (2)
- AET (1)
- Adenacarcinoma (1)
- Apoptosis (1)
- Biosynthesis (1)
-
- CAPK (1)
- CAPPs (1)
- Cancer (1)
- Capcase (1)
- Caspase 9 (1)
- Cathepsin D (1)
- Ceramide (1)
- Chemotherapy (1)
- Cytokines (1)
- Eicosanoid (1)
- Glycosaminoglycan (1)
- Growth factor (1)
- Hemorrage (1)
- Isoform (1)
- Mutagenesis (1)
- PP1 (1)
- PP2A (1)
- Phospholipases (1)
- Phospholipid (1)
- Prostanoid (1)
- Protaglandin (1)
- Protein (1)
- RNAi (1)
- Shock (1)
- Splice (1)
Articles 1 - 6 of 6
Full-Text Articles in Entire DC Network
Modulation Of Gene Expression By Tumor-Derived Mutant P53. Role Of Transactivation In Gain-Of-Function., Mariano J. Scian
Modulation Of Gene Expression By Tumor-Derived Mutant P53. Role Of Transactivation In Gain-Of-Function., Mariano J. Scian
Theses and Dissertations
It was hypothesized that the C-terminal sequences for mutant p53 would be required for oligomerization. and oligomerization may be critical for gain-of-function. An N-terminal deletion mutant of p53 that deletes amino acids 1-293 was used as a tool to perform hetero-oligomerization studies. This mutant retains the entire oligomerization domain but dispenses off the transactivation domain and a large portion of the sequence- specific DNA-binding domain. Co-transfection experiments show that p53 del. 1-293 forms hetero-oligomeric complexes with p53-D281G. Also. co-expression of p53 del. 1- 293 with p53-D281G inhibited p53-D28lG-mediated transactivation of the EGFR and MDRl promoters suggesting that hetero-oligomerization inactivates transcriptional …
Vimentin Overexpression Contributes To The Biological Properties Of Metastatic Head And Neck Cancer Cells, Rachel J. Paccione
Vimentin Overexpression Contributes To The Biological Properties Of Metastatic Head And Neck Cancer Cells, Rachel J. Paccione
Theses and Dissertations
Epithelial to mesenchymal transition occurs in the later stages of epithelial tumor progression, with cells expressing mesenchymal markers. Of these, the intermediate filament protein vimentin is frequently upregulated in metastatic carcinomas. Previously, microarray studies showed that the gene encoding vimentin is highly upregulated in metastatic HN12 cells compared to a related primary tumor cell line. In this study, we confirmed this difference using real-time quantitative PCR, western blot analysis, and immunostaining. Furthermore, EGF and TGF-β, growth factors that induce migration and invasion of HN12 cells, produced synergistic increases in vimentin expression. To assess the contribution of vimentin to the biological …
Identification Of The Rna Cis-Elements That Interact With Srp30a To Regulate The Alternative Splicing Of Caspase 9 Pre-Mrna, Prabhat Mukerjee
Identification Of The Rna Cis-Elements That Interact With Srp30a To Regulate The Alternative Splicing Of Caspase 9 Pre-Mrna, Prabhat Mukerjee
Theses and Dissertations
Studies have shown that the alternative splicing of caspase 9 and the phospho-status of SR proteins, a conserved family of splicing factors, are regulated by chemotherapy and de novo ceramide via the action of protein phosphatase-1 (PP1). Two RNA splice variants are derived from the caspase 9 gene, pro-apoptotic caspase 9a and anti-apoptotic caspase 9b, via alternative splicing by either the inclusion or exclusion of an exon 3, 4, 5, and 6 cassette. In this study, the link between SR proteins and the alternative splicing of caspase 9 was established. Sequence analysis of the exon 3, 4, 5, and 6 …
The Influence Of Lipid Composition On The Binding Of Ldl To Chondroitin 6-Sulphate, Wilma Espiritu
The Influence Of Lipid Composition On The Binding Of Ldl To Chondroitin 6-Sulphate, Wilma Espiritu
Theses and Dissertations
The interaction between low-density lipoprotein (LDL) and glycosaminoglycans is a key factor in atherosclerosis. The present study examines the characteristics of LDL and its binding properties with the main glycosaminoglycan of the vascular wall, chondroitin 6- sulphate (C6S). The compositional characteristics that were studied for each LDL sample were phase transition temperature, phospholipid content, free cholesterol content, cholesteryl ester content, triglyceride content, and size. Correlations of these characteristics with LDL-C6S binding were analyzed using a turbidity assay. Our results showed that there is no correlation between LDL-C6S binding and phase transition temperature, triglyceride content, or size. Strong correlations were present …
Identification Of The Pla2 Responsible For Prostanoid Synthesis In Response To Inflammatory Cytokines, Chaminda Fernando
Identification Of The Pla2 Responsible For Prostanoid Synthesis In Response To Inflammatory Cytokines, Chaminda Fernando
Theses and Dissertations
Preliminary studies from our laboratory showed that cPLA2α may be responsible for approximately 50-60% of the PGE2 production in response to inflammatory cytokines. Thus, we hypothesized that a closely-related PLA2 is responsible for 40-50% of the PGE2 produced in response to inflammatory cytokines. To this end, we utilized RNAi technology, extensively optimized, to down regulate the expression of closely-related isoforms of phospholipase A2 in A549 cells and used an enzyme linked immuno sorbent assay (ELISA) to quantitate the PGE2 produced. These studies found that cytosolic phospholipase A2α (cPLA2α) regulated 97.7% of the prostaglandin E2 (PGE2) produced in response to inflammatory …
Cytokine And Chemokine Profiles In A Rat Model Of Hemorrhagic Shock After Immuno-Modulation By Androstenetriol, Kristin E. Paccione
Cytokine And Chemokine Profiles In A Rat Model Of Hemorrhagic Shock After Immuno-Modulation By Androstenetriol, Kristin E. Paccione
Theses and Dissertations
Further understanding of the cellular and molecular mechanisms involved in traumatic injury, and how they are modulated during drug interaction, can facilitate novel treatment strategies for future trauma patients. We hypothesize that the pharmacological agent, Androstenetriol (AET), up regulates host immune response by modulating the continued expression of mediators, including cytokines. In a double-blinded experiment, rats were hemorrhaged, driven by volume or pressure conditions, then resuscitated with fluids and packed red blood cells following a subcutaneous injection of either vehicle or drug. Blood was collected at various time points and cytokine levels were determined by analyses with both multiplex and …