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Phytochemical Analysis And Biological Activities Of Cola Nitida Bark, Durand Dah-Nouvlessounon, Mamoudou H. Dicko Prof., Lamine Baba-Moussa

Pr. Mamoudou H. DICKO, PhD

The aim of this study is to investigate some biological effects of Cola nitida’s bark after phytochemical screening. The bark was collected, dried, and then powdered for the phytochemical screening and extractions. Ethanol and ethyl acetate extracts of C. nitida were used in this study. The antibacterial activity was tested on ten reference strains and 28 meat isolated Staphylococcus strains by disc diffusionmethod. The antifungal activity of three fungal strains was determined on the Potato-Dextrose Agar medium mixed with the appropriate extract. The antioxidant activity was determined by DPPH and ABTS methods. Our data revealed the presence of various potent …

Variability Of Vitamins B1, B2 And Minerals Content In Baobab (Adansonia Digitata) Leaves In East And West Africa, Hyacinthe Traore, Charles Parkouda, Adama Korbo, Diarra Compaoré/Sérémé, Mamoudou H. Dicko Prof., Jan J. Svejgaard, Bréhima Diawara

Pr. Mamoudou H. DICKO, PhD

The regional variability and age-age correlation on vitamin B1, vitamin B2 and minerals (Ca, Mg, P, K, Cu, Fe, Mn, Na, and Zn) concentration in baobab leaves were investigated. Baobab was cultivated from seeds from 11 african countries. Vitamins B1 and B2 content were assessed using microbiological VitaFast kits methods and minerals by atomic absorption and flame spectrometry methods. Overall, the results showed a higher content of vitamin B2 compared to vitamin B1 with the highest vitamin B2 content (1.04 +- 0.05 mg/100 g DM) from Senegal. The highest iron (Fe) content of 26.39 mg/100 g was found in baobab …

Purification And Characterization Of Sweet Potato (Ipomoea Batatas) Peroxidase, Mamouna Diao, Brice N’Cho Ayékoué, Crépin I. Dibala, Soumaïla Dabonné, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

Peroxidase (POX), a biotechnologically important enzyme was purified from bulb of Ipomoea batatas and characterized for use in the bioconversion of phenolic compounds from industrial wastewater. Purified peroxidase (POXp) was obtained using the gel-filtration chromatography and anion exchange chromatography. A final yield of 18 % was obtained with 20.5 as purification fold. Electrophoresis on SDS-PAGE and native-PAGE showed that POXp is monomeric with a molecular weight of 44 kDa. The optimum pH and optimum temperature were respectively 6.0 and 45 ° C. All enzyme activity was retained for 2 hours at pH values between 4.0 to 9.0. While the enzyme …

Characteristics, Composition And Oxidative Stability Of Lannea Microcarpa Seed And Seed Oil, Patrice Bazongo, Søren Nielsen, Adama Hilou, Mamoudou H. Dicko Prof., Vijai K. S. Shukla

Pr. Mamoudou H. DICKO, PhD

Proximate composition of seeds and main physicochemical properties and thermal stability of oil extracted from Lannea microcarpa seeds were evaluated. The composition of the seeds was: ash (3.11%), crude ol (6.90%), protein (21.14%), total carbohydrate (10.85%) and moisture (3.24%). Physicochemical properties of the oil were: refractive index, 1.473; melting point, 22.60°C; saponification value, 194.23 mg of KOH/g of oil; iodine value, 61.33 g of I2/100 g of oil; acid value, 1.21 mg of KOH/g of oil; peroxide value, 1.48 meq of O2/kg of oil and oxidative stability index, 43.20 h. Oleic (43.45%), palmitic (34.45%), linoleic (11.20%) and stearic (8.35%) acids …

Proximate Composition Of Traditional Local Sorghum Beer “ Dolo ” Manufactured In Ouagadougou, Fatouma Mohamed Abdoul-Latif, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

Dolo is a local beer manufactured from malted red sorghum grains. It is the most commonly consumed alcoholic beverage in Burkina Faso. Thirty samples of dolo, were collected from local markets in Ouagadougou and analyzed with respect to their proximate compositions and pH values. The average values of pH, dry matter and insoluble matter among samples were 3.5, 5.90 % and 0.85 %, respectively. Alcohol content of dolo samples was on average 2.3 % (v/v). The content of total proteins, carbohydrates and reducing sugars were 26, 38 and 10 µg/ml, respectively. Only traces of lipids were detected in all dolo …

Comparison Of Phenolic Compounds And Antioxidant Capacities Of Traditional Sorghum Beers With Other Alcoholic Beverages, Fatouma Abdoul-Latif, Romaric G. Bayili, Louis C. Obame, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

Thirty samples of sorghum beers “dolo” were selected from traditionally fermented household manufacturers from Burkina Faso. Dolo samples were screened for their total phenolic content, proanthocyanidins and putative antioxidant capacities, and were compared with industrial beers and wines. Total phenols were measured using the Folin-Ciocalteu method. Proanthocyanidins content were determined by the method of HCl-butanol hydrolysis. Antioxidant activities were evaluated both with 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and by the trolox equivalent antioxidant capacity (TEAC) using 2,2’-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid radical) (ABTS•+). The average contents of total phenols and proanthocyanidins were 506 μg GAE/ml of dolo and 45 μg APE/ml of dolo, respectively. An …

Composition And Antimicrobial Activities Of Lippia Multiflora Moldenke, Mentha X Piperita L. And Ocimum Basilicum L. Essential Oils And Their Major Monoterpene Alcohols Alone And In Combination, Mamoudou H. Dicko Prof., Aline Aline Lamien-Meda, Balé Bayala, Souleymane Tirogo, Chlodwig Chlodwig Franz, Johannes Johannes Novak, Roger Charles Roger Charles Nebié

Pr. Mamoudou H. DICKO, PhD

Essential oils from leaves of Lippia multiflora, Mentha x piperita and Ocimum basilicum from Burkina Faso were analysed by GC–FID and GC–MS. Major components were p-cymene, thymol, β-caryophyllene, carvacrol and carvone for L. multiflora, menthol and iso-menthone for M. x piperita and, linalool and eugenol for O. basilicum. The essential oils and their major monoterpene alcohols were tested against nine bacterial strains using the disc diffusion and broth microdilution methods. The essential oils with high phenolic contents were the most effective antimicrobials. The checkerboard method was used to quantify the efficacy of paired combinations of essential oils and their major …

Biochemical Characterization Of The Major Sorghum Grain Peroxidase, Mamoudou H. Dicko, Harry Gruppen, Riet Hilhorst, Alphons G. J. Voragen, Willen W. H. Van Berkel

Pr. Mamoudou H. DICKO, PhD

Phd Thesis: Wageningen University, 2005, Dr. Mamoudou H. Dicko "Endogenous Phenolics And Starch Mofying Enzymes As Determinants Of Sorghum For Food Use In Burkina Faso", Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

The objective of this thesis is to screen for biochemical determinants in sorghum varieties cultivated in Burkina Faso to identify the best sorghum varieties to be used as source of bioactive components or for specific local foods, e.g. “tô”, thin porridges for infants, granulated foods “couscous”, and local beers “dolo”. The results revealed that sorghum varieties have different contents of starch, amylose, amylopectin and that germination decreased more the content of amylose than amylopectin. While on average  amylase activity increased in all varieties by 2-20 fold,  amylase activity did not uniformly increase after germination and even decreased in …

Phd Thesis (Doctorat De Spécialité): Université De Ouagadougou, 1999. Dr. Mamoudou H. Dicko. Purification Et Propriétés Physico-Chimiques Des Enzymes De Curculigo Pilosa, Gladiolus Klattianus Et Boscia Senegalensis Catalysant L'Hydrolyse Des Polysaccharides (Amidon Et Béta-Glucanes), Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

The objective of this study was the research of novel and inexpensive sources of polysaccharides degrading enzymes such as amylases and glucanases from local plants in order to justify their biotechnological applications. The isolation of two l3-amylases and an endo-1,3-ß-D-glucanase was reached using common protein purification methods such as buffer extraction, ammonium sulfate fractionation, ionexchange and gel filtration chromatographies. The methods used were simple and easily reproducible, suggesting the possibilfty of large-scale production. ln the crude extract of Curculigo pilosa tuber, only ß-amylase was detected as starch degrading enzyme and its activity was approximately 282 Uig of fresh material. The …