Digital Commons Network^™

Stress-Induced Reinstatement Of Nicotine Preference Requires Dynorphin/Kappa Opioid Activity In The Basolateral Amygdala, Stephanie K. Nygard, Nicholas J. Hourguettes, Gabe G. Sobczak, William A. Carlezon, Michael R. Bruchas

Open Access Publications

UNLABELLED: The dynorphin (DYN)/kappa-opioid receptor (KOR) system plays a conserved role in stress-induced reinstatement of drug seeking for prototypical substances of abuse. Due to nicotine's high propensity for stress-induced relapse, we hypothesized that stress would induce reinstatement of nicotine seeking-like behavior in a KOR-dependent manner. Using a conditioned place preference (CPP) reinstatement procedure in mice, we show that both foot-shock stress and the pharmacological stressor yohimbine (2 mg/kg, i.p.) induce reinstatement of nicotine CPP in a norbinaltorphimine (norBNI, a KOR antagonist)-sensitive manner, indicating that KOR activity is necessary for stress-induced nicotine CPP reinstatement. After reinstatement testing, we visualized robust c-fos …

De Novo And Inherited Mutations In The X-Linked Gene Clcn4 Are Associated With Syndromic Intellectual Disability And Behavior And Seizure Disorders In Males And Females, E E Palmer, D K Grange, L Manwaring, Et Al

Open Access Publications

No abstract provided.

Adam17 Substrate Release In Proximal Tubule Drives Kidney Fibrosis, Eirini Kefaloyianni, Muthu Lakshmi Muthu, Jakob Kaeppler, Xiaoming Sun, Venkata Sabbisetti, Athena Chalaris, Stefan Rose-John, Eitan Wong, Irit Sagi, Sushrut S. Waikar, Helmut Rennke, Benjamin D. Humphreys, Joseph V. Bonventre, Andreas Herrlich

Open Access Publications

Kidney fibrosis following kidney injury is an unresolved health problem and causes significant morbidity and mortality worldwide. In a study into its molecular mechanism, we identified essential causative features. Acute or chronic kidney injury causes sustained elevation of a disintegrin and metalloprotease 17 (ADAM17); of its cleavage-activated proligand substrates, in particular of pro-TNFα and the EGFR ligand amphiregulin (pro-AREG); and of the substrates' receptors. As a consequence, EGFR is persistently activated and triggers the synthesis and release of proinflammatory and profibrotic factors, resulting in macrophage/neutrophil ingress and fibrosis. ADAM17 hypomorphic mice, specific ADAM17 inhibitor-treated WT mice, or mice with inducible …

Characterization Of Candidate Genes In Inflammatory Bowel Disease-Associated Risk Loci, Joanna M. Peloquin, Gautam Goel, Lingjia Kong, Hailiang Huang, Talin Haritunians, R. Balfour Sartor, Mark J. Daly, Rodney D. Newberry, Dermot P. Mcgovern, Vijay Yajnik, Sergio A. Lira, Ramnik J. Xavier

Open Access Publications

GWAS have linked SNPs to risk of inflammatory bowel disease (IBD), but a systematic characterization of disease-associated genes has been lacking. Prior studies utilized microarrays that did not capture many genes encoded within risk loci or defined expression quantitative trait loci (eQTLs) using peripheral blood, which is not the target tissue in IBD. To address these gaps, we sought to characterize the expression of IBD-associated risk genes in disease-relevant tissues and in the setting of active IBD. Terminal ileal (TI) and colonic mucosal tissues were obtained from patients with Crohn's disease or ulcerative colitis and from healthy controls. We developed …

Multiple Mechanisms For Processing Reward Uncertainty In The Primate Basal Forebrain, Noah M. Ledbetter, Charles D. Chen, Ilya E. Monosov

Open Access Publications

UNLABELLED: The ability to use information about the uncertainty of future outcomes is critical for adaptive behavior in an uncertain world. We show that the basal forebrain (BF) contains at least two distinct neural-coding strategies to support this capacity. The dorsal-lateral BF, including the ventral pallidum (VP), contains reward-sensitive neurons, some of which are selectively suppressed by uncertain-reward predictions (U(-)). In contrast, the medial BF (mBF) contains reward-sensitive neurons, some of which are selectively enhanced (U(+)) by uncertain-reward predictions. In a two-alternative choice-task, U(-) neurons were selectively suppressed while monkeys chose uncertain options over certain options. During the same choice-epoch, …

Using Diffusion Tractography To Predict Cortical Connection Strength And Distance: A Quantitative Comparison With Tracers In The Monkey, Chad J. Donahue, Stamatios N. Sotiropoulos, Saad Jbabdi, Moises Hernandez-Fernandez, Timothy E. Behrens, Tim B. Dyrby, Timothy Coalson, Henry Kennedy, Kenneth Knoblauch, David C. Van Essen, Matthew F. Glasser

Open Access Publications

UNLABELLED: Tractography based on diffusion MRI offers the promise of characterizing many aspects of long-distance connectivity in the brain, but requires quantitative validation to assess its strengths and limitations. Here, we evaluate tractography's ability to estimate the presence and strength of connections between areas of macaque neocortex by comparing its results with published data from retrograde tracer injections. Probabilistic tractography was performed on high-quality postmortem diffusion imaging scans from two Old World monkey brains. Tractography connection weights were estimated using a fractional scaling method based on normalized streamline density. We found a correlation between log-transformed tractography and tracer connection weights …

Asfotase Alfa Therapy For Children With Hypophosphatasia, Michael P. Whyte, Katherine L. Madson, Dawn Phillips, Amy L. Reeves, William H. Mcalister, Amy Yakimoski, Karen E. Mack, Kim Hamilton, Kori Kagan, Kenji P. Fujita, David D. Thompson, Scott Moseley, Tatjana Odrljin, Cheryl Rockman-Greenberg

Open Access Publications

No abstract provided.

Tmem184b Promotes Axon Degeneration And Neuromuscular Junction Maintenance, Martha R.C. Bhattacharya, Stefanie Geisler, Sara K. Pittman, Ryan A. Doan, Conrad C. Weihl, Jeffrey Milbrandt, Aaron Diantonio

Open Access Publications

UNLABELLED: Complex nervous systems achieve proper connectivity during development and must maintain these connections throughout life. The processes of axon and synaptic maintenance and axon degeneration after injury are jointly controlled by a number of proteins within neurons, including ubiquitin ligases and mitogen activated protein kinases. However, our understanding of these molecular cascades is incomplete. Here we describe the phenotype resulting from mutation of TMEM184b, a protein identified in a screen for axon degeneration mediators. TMEM184b is highly expressed in the mouse nervous system and is found in recycling endosomes in neuronal cell bodies and axons. Disruption of TMEM184b expression …

Functional Significance Of The Discordance Between Transcriptional Profile And Left Ventricular Structure/Function During Reverse Remodeling, Veli K. Topkara, Kari T. Chambers, Kai-Chien Yang, Huei-Ping Tzeng, Sarah Evans, Carla Weinheimer, Attila Kovacs, Jeffrey Robbins, Philip Barger, Douglas L. Mann

Open Access Publications

To elucidate the mechanisms for reverse LV remodeling, we generated a conditional (doxycycline [dox] off) transgenic mouse tetracycline transactivating factor-TRAF2 (tTA-TRAF2) that develops a dilated heart failure (HF) phenotype upon expression of a proinflammatory transgene, TNF receptor-associated factor 2 (TRAF2), and complete normalization of LV structure and function when the transgene is suppressed. tTA-TRAF2 mice developed a significant increase in LV dimension with decreased contractile function, which was completely normalized in the tTA-TRAF2 mice fed dox for 4 weeks (tTA-TRAF2

Provisional Tic Disorder: What To Tell Parents When Their Child First Starts Ticcing, Kevin J. Black, Elizabeth Rose Black, Deanna J. Greene, Bradley L. Schlaggar

Open Access Publications

No abstract provided.

Maturation Of Nav And Kv Channel Topographies In The Auditory Nerve Spike Initiator Before And After Developmental Onset Of Hearing Function, Kyunghee X. Kim, Mark A. Rutherford

Open Access Publications

No abstract provided.

Oncomodulin, An Ef-Hand Ca2+ Buffer, Is Critical For Maintaining Cochlear Function In Mice, Benton Tong, Aubrey J. Hornak, Stéphane F. Maison, Kevin K. Ohlemiller, M Charles Liberman, Dwayne D. Simmons

Open Access Publications

UNLABELLED: Oncomodulin (Ocm), a member of the parvalbumin family of calcium binding proteins, is expressed predominantly by cochlear outer hair cells in subcellular regions associated with either mechanoelectric transduction or electromotility. Targeted deletion of Ocm caused progressive cochlear dysfunction. Although sound-evoked responses are normal at 1 month, by 4 months, mutants show only minimal distortion product otoacoustic emissions and 70-80 dB threshold shifts in auditory brainstem responses. Thus, Ocm is not critical for cochlear development but does play an essential role for cochlear function in the adult mouse.

SIGNIFICANCE STATEMENT: Numerous proteins act as buffers, sensors, or pumps to control …

Getting To More Effective Weight Management In Antipsychotic-Treated Youth: A Survey Of Barriers And Preferences, Ginger Nicol, Elizabeth Worsham, Debra Haire-Joshu, Alexis Duncan, Julia Schweiger, Michael Yingling, Eric Lenze

Open Access Publications

No abstract provided.

Ability Of Serum Glial Fibrillary Acidic Protein, Ubiquitin C-Terminal Hydrolase-L1, And S100b To Differentiate Normal And Abnormal Head Computer Tomography Findings In Patients With Suspected Mild Or Moderate Traumatic Brain Injury, Lawrence M. Lewis, Et Al

Open Access Publications

No abstract provided.

Conjugation To The Sigma-2 Ligand Sv119 Overcomes Uptake Blockade And Converts Dm-Erastin Into A Potent Pancreatic Cancer Therapeutic, Kerri A. Ohman, Yassar M. Hashim, Suwanna Vangveravong, Timothy M. Nywening, Darren R. Cullinan, S. Peter Goedegebuure, Jingxia Liu, Brian A. Van Tine, Herve Tiriac, David A. Tuveson, David G. Denardo, Dirk Spitzer, Robert H. Mach, William G. Hawkins

Open Access Publications

No abstract provided.

Massively Parallel Cis-Regulatory Analysis In The Mammalian Central Nervous System, Susan Q. Shen, Connie A. Myers, Andrew E.O. Hughes, Leah C. Byrne, John G. Flannery, Joseph C. Corbo

Open Access Publications

No abstract provided.

Aromatase Inhibition Remodels The Clonal Architecture Of Estrogen-Receptor-Positive Breast Cancers, Christopher A. Miller, Yevgeniy Gindin, Charles Lu, Obi L. Griffith, Malachi Griffith, Dong Shen, Jeremy Hoog, Tiandao Li, David E. Larson, Mark Watson, Sherri R. Davies, Jacqueline Snider, Thomas Walsh, Graham A. Colditz, Katherine Deschryver, Richard K. Wilson, Elaine R. Mardis, Matthew J. Ellis, Et Al

Open Access Publications

No abstract provided.

Radiographic Comparison Between Cervical Spine Lateral And Whole-Spine Lateral Standing Radiographs, Moon Soo Park, Seong-Hwan Moon, Tae-Hwan Kim, Jae Keun Oh, Hwa Jun Kang, K. Daniel Riew

Open Access Publications

No abstract provided.

Isolated Oculomotor Nerve Palsy Resulting From Acute Traumatic Tentorial Subdural Hematoma, Victoria Cui, Timur Kouliev

Open Access Publications

No abstract provided.

Ramucirumab With Eribulin Versus Eribulin In Locally Recurrent Or Metastatic Breast Cancer Previously Treated With Anthracycline And Taxane Therapy: A Multicenter, Randomized, Phase Ii Study, Foluso Ademuyiwa, Et Al

Open Access Publications

No abstract provided.

Full-Thickness Wound Healing Using 3d Bioprinted Gelatin-Alginate Scaffolds In Mice: A Histopathological Study, Jing Liu, Jinghua Chi, Kaixi Wang, Xiaoping Liu, Jie Liu, Fang Gu

Open Access Publications

No abstract provided.

Functional Variants Of Human Papillomavirus Type 16 Demonstrate Host Genome Integration And Transcriptional Alterations Corresponding To Their Unique Cancer Epidemiology, Robert Jackson, Bruce A. Rosa, Sonia Lameiras, Sean Cuninghame, Josee Bernard, Wely B. Floriano, Paul F. Lambert, Alain Nicolas, Ingeborg Zehbe

Open Access Publications

No abstract provided.

Network Community Structure Alterations In Adult Schizophrenia: Identification And Localization Of Alterations, Dov B. Lerman-Sinkoff, Deanna M. Barch

Open Access Publications

No abstract provided.

Coding Variation In Angptl4, Lpl, And Svep1 And The Risk Of Coronary Disease, Aldi T. Kraja, Et Al

Open Access Publications

No abstract provided.

Pathogenic Mycobacteria Achieve Cellular Persistence By Inhibiting The Niemann-Pick Type C Disease Cellular Pathway [Version 1; Referees: 2 Approved, 2 Approved With Reservations], Daniel S. Ory, Et Al

Open Access Publications

No abstract provided.

Natriuretic Peptide Receptor-C Is Up-Regulated In The Intima Of Advanced Carotid Artery Atherosclerosis, Mohamed A. Zayed, Scott D. Harring, Dana R. Abendschein, Chandu Vemuri, Dongsi Lu, Lisa Detering, Yongjian Liu, Pamela K. Woodard

Open Access Publications

No abstract provided.

Treatment With Tumor-Treating Fields Therapy And Pulse Dose Bevacizumab In Patients With Bevacizumab-Refractory Recurrent Glioblastoma: A Case Series., George Ansstas, David D. Tran

Open Access Publications

No abstract provided.

The Effect Of Chloroquine On Immune Activation And Interferon Signatures Associated With Hiv-1, David Clifford, Et Al

Open Access Publications

No abstract provided.

Cancer Immunogenomics: Computational Neoantigen Identification And Vaccine Design, Jasreet Hundal, Christopher J. Miller, Malachi Griffith, Obi L. Griffith, Jason Walker, Susanna Kiwala, Aaron Graubert, Joshua Mcmichael, Adam Coffman, Elaine R. Mardis

Open Access Publications

No abstract provided.

Fostering Collaborative Research For Rare Genetic Disease: The Example Of Niemann-Pick Type C Disease, Steven U. Walkley, Cristin D. Davidson, Jonathan Jacoby, Philip D. Marella, Elizabeth A. Ottinger, Christopher P. Austin, Forbes D. Porter, Charles H. Vite, Daniel S. Ory

Open Access Publications

No abstract provided.