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Masters Theses

1997

Biology

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Isolation And Characterization Of Cadmium Hypertolerant Mutants Of Arabidopsis Thaliana, Santiago Xavier Navarro Dec 1997

Isolation And Characterization Of Cadmium Hypertolerant Mutants Of Arabidopsis Thaliana, Santiago Xavier Navarro

Masters Theses

Cadmium is an environmental pollutant which accumulates by deposition from anthropogenic activities. Plants readily take-up cadmium from soil and is concentrated in shoot tissues. Plant and animal systems are affected detrimentally by the exposure to cadmium and are known to produce metal binding peptides as part of detoxification mechanisms. In an attempt to find alternative methods of soil cleanup, efforts are being undertaken to isolate heavy metal accumulating plants. Alternatively, to reduce the accumulation of cadmium in food, plants that exclude heavy metals are also being sought. The objective of this project was to isolate mutants which can be used …


Effect Of Insulin And Vanadate On Sre-Mediated C-Fos Expression And Jnk Activation, Francisco J. Berguido Dec 1997

Effect Of Insulin And Vanadate On Sre-Mediated C-Fos Expression And Jnk Activation, Francisco J. Berguido

Masters Theses

Insulin mimetics are compounds that mimic the action of insulin in the body and are currently being studied as possible therapeutic agents against diabetes. Vanadium has been identified as an element with insulin-like properties with regard to metabolic processes such as lowering blood glucose levels. Few studies have implicated vanadium in mitogenic actions such as cell growth and differentiation. We studied the action of vanadate on the expression of the insulin-induced SRE, a cis-element of the well-characterized mitogenic gene c-fos. We measured this expression by means of a plasmid containing the reporter gene luciferase linked to the sequences from c-fos …


Activation Of Signal Proteins By Insulin And Selenite In Primary Rat Hepatocytes, Ginny Lynn Garlock Dec 1997

Activation Of Signal Proteins By Insulin And Selenite In Primary Rat Hepatocytes, Ginny Lynn Garlock

Masters Theses

Insulin has a wide range of metabolic and mitogenic effects in cells. The mechanism of insulin action in controlling these events is not well understood, but involves, at least in part, a complex phosphorylation cascade. Selenate has been shown to mimic insulin on several metabolic processes, including the regulation of glucose-6-phosphate dehydrogenase (G6PDH) gene expression. Its mechanism of action, however, is not known. To investigate the mechanisms of insulin and selenate action on the regulation of G6PDH, signal proteins from primary rat hepatocytes were first studied. Insulin and sodium selenate similarly increased tyrosyl phosphorylation of the β-subunit of the insulin …


The Role Of Free Radicals In The Plant's Response To Pathogen Attack In Tobacco, Phillip L. Peters Aug 1997

The Role Of Free Radicals In The Plant's Response To Pathogen Attack In Tobacco, Phillip L. Peters

Masters Theses

Previous studies have implicated the involvement of free radicals, specifically reactive oxygen species (ROS), as a key component in plant response to pathogen attack. In this study we examined the effect free radical scavenging compounds have on lesion area, salicylic acid levels, catalase, and superoxide dismutase in response to inoculation with Tobacco Mosaic Virus (TMV), a viral pathogen. Hydroponically grown Nicotiana tabacum (Xanthi nc) were pretreated with one of two antioxidants, either purpurogallin (PPG) or N-acetyl cysteine (NAC). Antioxidants were supplied to the plant via the root system and the leaves were subsequently challenged by inoculation with theTMV. Leaf material …


Life History Characteristics Of Daphnia Lumholtzi And Its Susceptibility To An Invertebrate Predator, Eden L. Rawski Jan 1997

Life History Characteristics Of Daphnia Lumholtzi And Its Susceptibility To An Invertebrate Predator, Eden L. Rawski

Masters Theses

No abstract provided.