Digital Commons Network^™

Development Of Novel Vaccines For Campylobacter Control In Poultry, Lindsay Ann Jones

Masters Theses

Campylobacter is the leading bacterial cause of human enteritis in developed countries. Human campylobacteriosis is commonly associated with consumption of undercooked, contaminated chicken, a natural host of Campylobacter. Thus, control of Campylobacter colonization in poultry at the farm level would reduce the risk of human exposure to this pathogen. Vaccination is an attractive intervention measure to mitigate Campylobacter in poultry. Our recent studies have demonstrated that the outer membrane proteins CmeC (an essential component of CmeABC multidrug efflux pump) and CfrA (ferric enterobactin receptor) are feasible candidates for immune intervention against Campylobacter. By targeting these two promising vaccine …

A Novel Approach To Multiphysics Modeling Of Heat And Mass Transfer In Porous Media, Seth Allen Pemberton

Masters Theses

This thesis aims to investigate conjugate heat and mass transfer in porous media with an emphasis on textiles. Both hygroscopic materials, those that absorb water vapor, and non-hygroscopic materials are examined. A model was developed that utilizes COMSOL’s equation-based partial differential equation (PDE) interface which allows the user to input any equation(s) to be solved. By the use of experimental and numerical data each part of the model, i.e. flow field, gas diffusion, convection and vapor absorption, is verified. The accuracy of the equation-based unsteady flow field is verified by modeling the flow over a circular cylinder and extracting the …

Cobb Creek Church: Changing Perspectives In A Serpent-Handling Congregation In East Tennessee, Michael Noel Reid

Masters Theses

In the last year, the traditional practice of handling venomous snakes in Pentecostal church services has returned to the forefront of popular media attention. With the death of renowned handler Randy “Mack” Wolford in West Virginia in May, the news has been rife with stories of the century-old tradition. New, younger groups of handlers have also been instrumental in raising attention to the practice. One congregation in particular has been a key focus for media outlets around the nation. The Cobb Creek Church of God has been featured in The Tennessean, The Wall Street Journal, USA Today, …

The Economics Of Grazing Beef And Dairy Cattle On Native Warm-Season Grasses In Tennessee, Joe Kenneth Lowe

Masters Theses

Two separate studies focusing on the economics and animal performance of grazing native warm-season grasses (NWSGs) are presented in this thesis. In the first study, the first objective was to determine if there was a difference in net returns to full-season grazing of beef steers on switchgrass (SG), big bluestem and indiangrass (BBIG), and eastern gamagrass (EG). The second objective was to determine the price of biomass a producer would need to receive to breakeven between full-season grazing beef steers and early-season grazing with a biomass harvest (i.e., a dual purpose grazing and biomass production system) for SG, BBIG, and …

Temperament In Mothers With Borderline Personality Disorder And In Their Young Children Aged 4-7, Christina Gabriela Mena

Masters Theses

In this study we sampled mothers with Borderline Personality Disorder (BPD) and their young children ages 4-7 (n = 36), as well as normative comparisons (n = 34). We assessed temperament in both mothers and their children. Mothers reported on their own and on their children’s temperament. Controlling for maternal current major depressive disorder and education, mothers with BPD reported more negative affectivity, less effortful control, and less positive affect than did normative comparison mothers. Children whose mothers had BPD had more negative affectivity (fear and frustration) and less effortful control, but not less smiling and laughter. When …

Small Scale Population Structure Of Pityopsis Ruthii And P. Graminifolia Var. Latifolia, Sarah Lynn Boggess

Masters Theses

Pityopsis graminifolia var. latifolia (Michx.) Small var. latifolia (Fern.) or narrowleaf silkgrass is an herbaceous perennial native to the southeastern United States and northern Central America. Pityopsis ruthii (Small) Small is an endangered plant endemic to the Hiwassee and Ocoee Rivers in Polk County, Tennessee. Little is known about the genetic diversity and population structure of both Pityopsis species as well as the phylogenic relationships between the species. In this study, 16 microsatellite loci were used to assess genetic diversity and population structure of 261 individuals of P. ruthii (n=167) and P. graminifolia var. latifolia (n=76). Pityopsis ruthii is …

A Framework To Predict High Risk Roadways For Pedestrians In Tennessee, Zane Hunter Pannell

Masters Theses

Pedestrians and bicyclists are a class of vulnerable road users that are often over-represented in incapacitating injury or fatal crash statistics. Because non-motorized trips are vital to many urban and rural residents for utility or recreation and exercise, it is essential to identify safety deficiencies in our existing transportation infrastructure to address rising injuries and fatalities among this group of road users. As the economy continues to struggle and fuel prices remain high, many cities and rural transportation agencies are seeing large increases in bicycling, walking, and transit ridership. While passenger car fatalities have shown sharp declines in the last …

Effect Of Disjoining Pressure And Working Fluid On Multi-Scale Modeling For Evaporative Liquid Metal Capillary, Hunju Yi

Masters Theses

This research presents a new multiscale model of an evaporating liquid metal capillary meniscus under nonequilibrium evaporation sustaining a nonisothermal interface.

The primary investigation is elaborated on to examine the critical role of the disjoining pressure, which consists of both the traditional van der Waals component and a new electronic pressure component, for the case of liquid metals. The fully extended dispersion force is modeled along with an electronic disjoining pressure component that is unique to liquid metals attributing to their abundant free electrons. For liquid alkali metals (sodium and lithium), as a favorable coolant for high temperature two-phase devices, …

Adolescents’ Definitions Of Cheating In Romantic Relationships, Jerika C. Norona

Masters Theses

Cheating is a common occurrence in dating relationships. However, less is known about cheating in adolescence, a time when many individuals first experience romantic relationships. An important initial step for research is examining how adolescents define cheating in their romantic relationships. The present study used Thematic Analysis, a qualitative analytic method, to explore adolescents’ definitions of cheating and how these definitions might differ across age and gender. Furthermore, the present study examined patterns that emerged within definitions. Results indicate that definitions of cheating included a range of behaviors, such as engaging in physical activity, romantic/intimate involvement, spending time with, talking …

Hydrogeology Of The Little River Animal Agriculture Environmental Research Unit And Impacts Of Dairy Operations On Groundwater, Robert Wesley Hunter

Masters Theses

This thesis describes the development of an integrated hydrogeologic/hydrologic site assessment and groundwater/surface water quality monitoring program at the University of Tennessee – Little River Dairy Farm, located near Townsend, TN. Hydrologic/hydrogeologic investigations of streams and groundwater at the site have been underway for more than 5 years, and these are expected to provide background data for assessing impacts of dairy wastes. The lower half of the ~180 ha site consists of low-relief fields used for row crops, which are underlain by 4 – 9 m of alluvial deposits on top of black shale or limestone that include sinkhole features. …

Arm Inductance And Sub-Module Capacitance Selection In Modular Multilevel Converter, Yalong Li

Masters Theses

Arm inductor and sub-module (SM) capacitor are two key components in the modular multilevel converter (MMC). Optimizing the selection of arm inductance and sub-module capacitance is thus critical for the converter design. This report aims at developing a selection principle for arm inductance and sub-module capacitance in MMC.

Arm inductors in MMC are used to limit the circulating current which flows within the converter. The switching frequency harmonic is found to be the dominant component in the circulating current when an active circulating current suppressing controller is implemented. The analytical relationship between the arm inductance and switching frequency circulating current …

An Automated Finite Element Analysis Framework For The Probabilistic Evaluation Of Composite Lamina Properties, Jonathan Phillips Weigand

Masters Theses

This thesis outlines the development of computational modeling tools used to predict the elastic properties of composite lamina from representative volume elements (RVE) using numerical methods. The homogenization approach involves the use of Gauss’s Theorem to simply the average volumetric strain integral into a surface integral containing which is defined by surface displacements and their direction. Simulations of RVEs under specific loading conditions (longitudinal tension or shear and transverse tension or shear) are then performed in the software package ABAQUS to obtain the surface displacements. It was found that obtaining quality meshes and applying periodic boundary conditions for each RVE …

The Relationships Between Internalized Heterosexism, Spirituality, And Mental Health In Lesbian, Gay, And Bisexual Young Adults, Jon Raymond Bourn

Masters Theses

Minority stressors like internalized heterosexism have been found to be related to suicidality among lesbian, gay, and bisexual (LGB) individuals (e.g., Savin-Williams & Ream, 2003). Additional research is needed, however, to better understand the factors that may serve as moderators (i.e., protective factors) in the relationships between minority stressors and negative mental health outcomes, such as depression and suicidality (e.g., Szymanski et al., 2008). The current study attempted to examine the relationships between internalized heterosexism and two negative mental health outcomes associated with suicide, psychache (defined as unbearable psychological pain) and depression, in a sample of LGB young adults. Given …

Characterization Of Heat Transfer Coefficient Uncertainty In Support Of High Temperature Probe Measurement Technology, Marcus S. Conner

Masters Theses

The development of new materials and processes have enabled defense, industrial, and research devices that operate in high temperature environments. Measurement technology must keep up with the demand of these environments.

The objective of this work is to provide a correlation between the heat transfer coefficient (and Nusselt Number) and the flow Reynolds number (and Prandtl number) for a truncated cylindrical probe. The correlation provides reduced uncertainty for materials whose heat transfer coefficient is not well defined. The configuration for the experiment uses the University of Tennessee Space Institute’s (UTSI) blow down air supply system discharging into a duct and …

Wouldn't Future People Like To Know? A Compensation-Based Approach To Global Climate Change, Trevor Grant Hedberg

Masters Theses

Anthropogenic global climate change (GCC), understood as changes to the Earth’s climate system resulting from greenhouse gas emissions caused by human beings, has emerged as one of the most pressing environmental problems in human history. Proposed responses to climate change typically focus on either mitigation or adaptation. Mitigation refers to the process of lessening the effects of GCC, most often by reducing our emissions of greenhouse gases. Adaptation refers to the process of helping those who will be adversely affected by GCC adapt to the environmental changes to avoid being harmed. There is, however, a third approach to the issue …

Exodus Concerto For Guitar And Chamber Orchestra, Spencer Joel Kappelman

Masters Theses

Exodus is a four-movement composition for solo guitar accompanied by a chamber orchestra. This piece is composed in partial fulfillment of the requirements of the degree Master of Music with a concentration in Music Composition from the University of Tennessee, Knoxville. Exodus was composed during the 2010-2012 academic years.
This paper provides a narrative analysis of Exodus in terms of its musical content, and relationships to other composers of the last century. Similarities to these composers refer to form, orchestration, melody, harmony, rhythm, and meter.

Demonstration Of A Targeted Proteome Characterization Approach For Examining Specific Metabolic Pathways In Complex Bacterial Systems, Adam Justin Martin

Masters Theses

Multiple Reaction Monitoring (MRM) is a powerful tandem mass spectrometry (MS/MS) tool frequently implemented in proteomic studies to provide targeted analysis of proteins and peptides. The selectivity that MRM delivers is so strong that it provides the quadrupole mass spectrometers (QQQ), on which it is commonly employed, with pertinence to proteomic studies that they would otherwise lack for their relatively low resolution. Additionally, this increased level of selectivity is sufficient enough to supplant complicated fractionation techniques, additional dimensions of chromatography, and 24 hour long MS/MS experiments in simplistic biological samples. But there is a deficiency of evidence to determine the …

Environmental Constraints On Cyanomyophage Abundance In The Subtropical Pacific Ocean, Tiana Maria Pimentel

Masters Theses

Viruses are abundant in the world’s oceans and are thought to be important participants in marine biogeochemical cycling. Of these viruses, cyanophages are considered especially important because they infect and lyse cyanobacteria, which are some of the main primary producers in marine environments. Cyanophages are thought to influence the abundance and diversity of cyanobacterial populations and impart significant mortality, thereby affecting primary productivity and microbial community structure. Despite their ecological relevance, little is known about how environmental factors shape cyanophage abundance and diversity over large temporal and spatial scales. To address this gap in knowledge, seawater samples were collected during …

Personal And Group Environment Factors Of Water Polo Players, Shelby Morgan Reyes

Masters Theses

This study examined to see if Division I female water polo players (n = 113) had a distinct personality profile when compared to their counterpart of other female college students (n = 170). Also, this study analyzed to see the impact personality traits and team cohesion variables had on overall athletic satisfaction in female water polo players. The measures used were as follows: for personality, the Personal Style Inventory for College Students (PSI, Lounsbury & Gibson, 2008); for team cohesion, Group Environment Questionnaire (GEQ, Carron, Widmeyer, & Brawley, 1985) ; and for athletic satisfaction, Athletes Satisfaction Questionnaire (ASQ, Riemer & …

Bovine Mammary Interleukin-8 Receptor Expression And Genetic Association With Streptococcus Uberis Based Mastitis., Lydia Jean Siebert

Masters Theses

Mastitis is an economically distressing disease in the dairy industry. Bacterial pathogens enter the gland and encounter cell types that release immune mediators including interleukin (IL)-8. IL-8 has two membrane bound receptors: CXCR1 and CXCR2. CXCR1 and CXCR2 are expressed on neutrophils and other cell types in other species but their expression is unknown in the bovine mammary gland. To test this, mammary tissue samples from six Holstein dairy cows were subject to dual immunofluorescence with bovine specific CXCR1 and CXCR2 antibodies and cell type markers. CXCR1 was expressed on alveolar epithelial cells, fibroblasts, and leukocytes. CXCR2 expression was identified …

Molecular Dynamics Simulations Of Cascade Evolution Near Pre-Existing Defects, Nathan Allen Capps

Masters Theses

Radiation damage causes significant changes to material microstructure and properties as a result of the processes of atomic defect creation followed by the inherently multiscale defect diffusion and reaction processes. In particular, the overlap of displacement cascades is believed important in the development of visible defect clusters in thin film, in-situ ion irradiation studies. In this work, we use molecular dynamics simulations to investigate how impurities and damage induced by displacement cascades impact damage creation as well as the mobility of a pre-existing interstitial-type dislocation loop in BCC iron. It is well known that impurities, such as oxygen, carbon, and …

Practitioner Rankings Of Entry-Level Competencies For Public Health Nutrition, Courtney Teal Schand

Masters Theses

Background: The Association of Graduate Programs in Public Health Nutrition’s graduate competencies were updated and refined by an expert panel and a survey of Public Health Nutrition (PHN) academicians, which resulted in 58 proposed competencies in five domains, Food and Nutrition Science, Advocacy, Research and Evaluation, Communication and Cultural Competency, and Management and Leadership.

Objectives: The objectives of this study were 1) to determine if differences existed in calculated domain scores of practitioner rankings of essentiality for the PHN graduate competencies by demographic variables of interest (current position, years of experience, education, and/or intent to retire); 2) to determine the …

Issues Of Commingling Within The Gold Mine Site (16ri13) Collection: Adult Human Humeri And Tibiae, Kinsey Brett Stewart

Masters Theses

Gold Mine (16RI13) is a Troyville ossuary mound site (circa CE 825) in northeastern Louisiana. Approximately 10-20% of the primary mound (Mound A) was excavated over the course of three field seasons (1978-1980), yielding a host of human skeletal remains. Extensively commingled secondary burials make up the majority of interments. The number of individuals represented within the collection (N) has been estimated at 150+ (McGimsey 2004:214), but attempts to quantitatively determine N have produced varied results. Formal analysis of the skeletal collection is complicated by the loss of provenience for many remains as well as additional post-excavation fragmentation …

Internalized Heterosexism, Social Support, And Career Development In Lesbian, Gay, And Bisexual Undergraduate And Graduate Students: An Application Of Social Cognitive Career Theory, James Edward Arnett

Masters Theses

Using a the framework of Social Cognitive Career Theory (Lent, Brown, & Hackett, 1994), we examined the relationships between one potential career-related barrier, internalized heterosexism (IH), and social support on career decision-making self-efficacy (CDMSE) and vocational outcome expectations in lesbian, gay, and bisexual undergraduate and graduate students. Specifically, we predicted that internalized heterosexism would be negatively related to CDMSE and vocational outcome expectations, and that social support would serve as a buffer that moderates these relationships. Results indicated that IH and social support were both unique predictors of outcome expectations. There was also a significant interaction effect between IH and …

Spectroscopic, Thermal, And Physical Analysis Of The Raw Materials In Europium Doped Cesium Strontium Iodide Scintillator, Bonnie Dell Blalock

Masters Theses

Scintillators are widely used in homeland security applications that utilize gamma-ray spectroscopy. An ideal scintillator for this purpose should possess a high effective atomic number, high light yield, and good energy resolution. The scintillator CsSrI₃: Eu [cesium strontium iodide: europium], recently developed at the Scintillation Materials Research Center, has excellent properties. Recent efforts have been made to evaluate the basic thermal properties and IR spectra of the compound’s raw materials as part of a project to improve the quality of grown crystals. Fourier transform infrared spectroscopy of high purity anhydrous CsI [cesium iodide], SrI₂ [strontium iodide], and …

The Impact Of Rumination Induction On Iq Performance, Kerry Margaret Cannity

Masters Theses

Performance deficits on cognitive tasks have been demonstrated consistently in depressed and anxious individuals. Processing efficiency theory asserts that these deficits might be accounted for by task-irrelevant processes, including the negative impact of rumination. This study was designed to better understand the relationship between cognitive deficits and depression by creating a ruminative state in healthy control subjects to determine if they would exhibit performance deficits similar to those observed in patients with depression. Specifically, the effect of rumination induction on select subtests of the Wechsler Adult Intelligence Scale – Fourth Edition (WAIS-IV) was examined. Participants were college students with no …

Synthesis Of A Bifunctional Heterogeneous Catalyst For The Conversion Of Lignocellulosic Biomass Into Precursors For Alternative Fuels And Fine Chemicals, Matthew David Dembo

Masters Theses

The overall goal of the research described here is to develop synthetic routes to bifunctional reagents that can be tethered into silicate matrices derived from the tin functionalized cube, Si₈O₂₀(SnMe₃)₈. The critical functional group required for reaction with cubic silicate building block is a chlorosilane, and we additionally wish to incorporate both acid and ionic liquid groups. Once in hand, a crosslinking metathesis reaction will incorporate these reagents into porous silicate matrices derived from the Si₈O₂₀ cube, creating platforms with both acid and ionic liquid groups tethered to their …

An On-Chip Transformer-Based Digital Isolator System, Cory Lynn Fandrich

Masters Theses

An on-chip transformer-based digital isolator has been designed, fabricated, and tested. This isolation technique is designed to function between a low voltage microcontroller and a potentially high-voltage power control system. The isolator’s isolation capability is determined by two factors, the RMS blocking voltage strength and common-mode transient immunity. The integrated circuit solution is designed in a high-temperature capable SOI process.

The on-chip transformer size is minimized by utilizing high frequency voltage pulses. A small transformer and overall small chip footprint of the design are favorable for integration into a larger system. The isolator is a two chip solution, an isolated …

The Performance Of Bacterial Phytosensing Transgenic Tobacco Under Field Conditions, Michael Harrison Fethe

Masters Theses

Currently the platforms for wide-area detection of environmental contamination are limited. Therefore, there is interest in developing new platforms, especially for use in crop plants to detect and report the presence of biotic and abiotic stress agents. A biosensor uses a biological organism or substrate to detect the presence of an elicitor (i.e., heavy metal, TNT, or bacteria). The foundational groundwork to create biosensors in transgenic plants exists. The creation of bacterial phytosensing transgenic tobacco containing an orange fluorescent protein (OFP) reporter driven by synthetic pathogen-inducible promoters provides a fluorescent signal when infected with phytopathogens for earlier detection in the …

Antimicrobial Activity Of Essential Oils And Their Components Against Lactic Acid Bacteria, Laurel Dunn Gann

Masters Theses

Efficacy of plant essential oils against spoilage lactic acid bacteria was examined using two different study methods with the goal of determining minimum inhibitory (MIC) and minimum lethal concentrations (MLC) of the essential oils. The initial study included the incorporation of the essential oils, or their major constituents, into agar to allow uniform dispersion of the substance throughout an agar surface. Individual cultures of nine lactic acid bacteria species (Pediococcus acidilactici, Pediococcus damnosus, Lactobacillus fermentum, Lactobacillus fructivorans, Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus plantarum, Leuconostoc mesenteroides, and Leuconostoc citrovorum) were spot …