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Assessing The Effects Of The Spring Hunting Season Start Date On Wild Turkey Seasonal Productivity And Hunter Behavior, Joseph Quehl
Assessing The Effects Of The Spring Hunting Season Start Date On Wild Turkey Seasonal Productivity And Hunter Behavior, Joseph Quehl
Masters Theses
Many states throughout the Southeast have documented declines in wild turkey (Meleagris gallopavo) harvest and estimates of recruitment in poult-per-hen ratios. Wild turkey populations are driven by seasonal productivity, so the decline in these parameters may indicate a decline in the overall population. One hypothesis as to why we are seeing a reduction in productivity and a potential population decline is that the spring hunting season is disrupting the reproductive cycle by harvesting too many males before they have had the opportunity to breed, or by harvesting dominant males and disrupting the social hierarchy of the flock. Our …
Evaluation Of Forage Nutritive Value And Dry Matter Yield Of Stockpiled Tall Fescue Across Plant Hardiness Zones 7 And 8, Malerie Elizabeth Fancher
Evaluation Of Forage Nutritive Value And Dry Matter Yield Of Stockpiled Tall Fescue Across Plant Hardiness Zones 7 And 8, Malerie Elizabeth Fancher
Masters Theses
Pastures in Tennessee comprise mainly tall fescue [Schedonorus arundinaceus (Schreb.) Dumort.], a cool-season perennial grass. Tennessee producers feed hay for an average of 143 days per year, there is interest in utilizing stockpiling to extend the grazing season. Although this practice has been proven to reduce hay needs (Freeman et al., 2019), it is vital to maintain up-to-date recommendations using data from across the southeastern US to inform nutritional management decisions. The objective of this study is to determine the nutritive value and dry matter yield of stockpiled tall fescue across plant hardiness zones 7 and 8. Fourteen farms …
Assessing The Impact Of The Tennessee Equine Industry, Olivia Watson
Assessing The Impact Of The Tennessee Equine Industry, Olivia Watson
Masters Theses
The Tennessee equine industry is alive and prevalent across the entire state. However, due to the expansiveness of the industry, lack of consistent record keeping, and large discrepancies among reporting agencies, it has proven to be difficult to accurately account for the total impact of the industry in terms of population, demographics, and overall economic impact. The objective of this study was to 1) determine population demographics and economic impact of the equine industry in Tennessee and 2) determine the public perception on the addition of live equine racing within the state. An anonymous, online assessment was developed (March-August 2022; …
Production And Characterization Of Monoclonal Antibodies To Xenopus Proteins, Brett A. Horr
Production And Characterization Of Monoclonal Antibodies To Xenopus Proteins, Brett A. Horr
Masters Theses
Monoclonal antibodies are powerful and versatile tools that enable the study of proteins in diverse contexts. They are often utilized to assist with identification of subcellular localization and characterization of the function of target proteins of interest. However, because there can be considerable sequence diversity between orthologous proteins in Xenopus and mammals, antibodies produced against mouse or human proteins often do not recognize Xenopus counterparts. To address this issue, we refined existing mouse monoclonal antibody production protocols to generate antibodies against Xenopus proteins of interest. Here, we describe several approaches for the generation of useful mouse anti-Xenopus antibodies to multiple …
Management And Investment: Two Pillars Of Automatic Milking Systems Efficiency, Abby N. Mccalmon
Management And Investment: Two Pillars Of Automatic Milking Systems Efficiency, Abby N. Mccalmon
Masters Theses
Fetch cows, dairy cattle that do not voluntarily enter automated milking systems (AMS), increase labor costs, and decrease efficiency. The objective of this study was to describe fetch cows based on fetch lists and herd-management software data. This study was conducted on a commercial dairy farm (n = 510 cows) using 8 AMS from October 1, 2021, to May 10, 2022. Data were collected via PCDART, DairyComp, and a proprietary interface. Two fetch categories by cow, EverFetch (EF) and NeverFetch (NF), and three status groups by cow by day, True Fetch (on fetch list …
Using Novel Phenotypes To Understand And Predict Fertility In Beef Cows, Cassidy Catrett
Using Novel Phenotypes To Understand And Predict Fertility In Beef Cows, Cassidy Catrett
Masters Theses
Reproduction plays a major role in the production efficiency of livestock species. However, cow-centric reproductive traits are lowly heritable and are not expressed until later in an animal’s lifetime, making phenotypic selection ineffective at generating genetic gain. We can generate genetic progress by focusing selection on the genetic component of reproductive traits using Expected Progeny Differences (EPDs). We used the American Simmental Association’s performance and Total Herd Enrollment data, made up of 533,155 calving records from 303,158 females (132,403 cows and 170,755 heifers), 33,732 of which are genotyped, to develop two continuous and two discrete phenotypes focused on quantifying early …
Pregnancy Influences On The Rumen Environment Of Angus Heifers Differing In Feed Efficiency, Miranda Gabrielle Martin
Pregnancy Influences On The Rumen Environment Of Angus Heifers Differing In Feed Efficiency, Miranda Gabrielle Martin
Masters Theses
With an expected increase in population by 2050, the demand for more animal protein will increase. To improve the sustainability of US cattle production, producers and researchers have historically focused on improving the feed efficiency of steers, producing a more marketable beef product. However, without a successful pregnancy, there would be no marketable animal to feed. The objective of this study was to examine the physiological changes that occur during pregnancy and their impact on the rumen microbial communities and the correlation to feed efficiency status in Angus heifers. Utilizing 17 cannulated Angus heifers, feed efficiency data was previously collected …
Consistency Is Key: Investigating Vocal Consistency In Field Sparrows, Stephanie Stanton
Consistency Is Key: Investigating Vocal Consistency In Field Sparrows, Stephanie Stanton
Masters Theses
No abstract provided.
Serotonin And Its Effects On The Chronic Stress Response In Crayfish Following Nonylphenol Exposure, Tara M. Olen
Serotonin And Its Effects On The Chronic Stress Response In Crayfish Following Nonylphenol Exposure, Tara M. Olen
Masters Theses
Current research suggests that stress in both vertebrates and invertebrates is modulated in part by the neurotransmitter serotonin. It has been shown that crayfish can function as a good neurophysiological model, as they have less complex neurological systems than vertebrates, so mechanistic causes for stress can be more readily studied and understood. One potential stressor for aquatic species such as crayfish would be nonylphenol, a hydrophobic chemical used in agricultural products that can make its way into the water supply due to agricultural runoff. Nonylphenol can lead to physiological and behavioral impairments in crayfish and these impairments likely induce stress …
Staphylococcal Surface Proteins As Vaccine Candidates For The Control Of Staphylococcal Mastitis In Dairy Cows, Jessica Jean Vidlund
Staphylococcal Surface Proteins As Vaccine Candidates For The Control Of Staphylococcal Mastitis In Dairy Cows, Jessica Jean Vidlund
Masters Theses
Bovine mastitis, an inflammation of the mammary gland, is the single most costly disease of dairy cattle. Staphylococci, a major bacteria that cause bovine mastitis, are commonly divided into two major groups: Staphylococcus aureus and non-aureus staphylococci (NAS), previously known as coagulase-negative Staphylococcus species (CNS). Current mastitis control programs are not fully effective against staphylococcal mastitis and antibiotics are not sustainable due to limited success and the emergence of antimicrobial resistant bacteria. Non-antibiotic sustainable control tools, such as effective vaccines, are critically needed. Our lab developed and evaluated Staphylococcus aureus surface associated protein (SASP) and Staphylococcus chromogenes surface associated protein …
Rumen Biogeographical Regions And Their Impact On Microbial And Metabolome Variation, Macey P. Soltis
Rumen Biogeographical Regions And Their Impact On Microbial And Metabolome Variation, Macey P. Soltis
Masters Theses
The rumen microbiome is a complex microbial network critical to the health and nutrition of its host, due to their inherent ability to convert low-quality feedstuffs into energy. In rumen microbiome studies, the ventral sac is sampled from due to ease of access and repeatability. However, anatomical musculature demarcates the rumen into five sacs which may support distinct microbial communities. The distinction among the microbes may generate functional variation among the rumen microbiome, thus, specialized tasks within different sacs. The objective of this study was to determine the rumen liquid metabolome and epimural, planktonic, and fiber-adherent bacterial communities among each …
Predicting Immunization Status At Arrival In Tennessee Stocker Calves, Claire E. Hunkler
Predicting Immunization Status At Arrival In Tennessee Stocker Calves, Claire E. Hunkler
Masters Theses
Bovine respiratory disease is a multifaceted disease with agent, host, and environmental factors. Stocker calves are at high risk of contracting the disease through many stressors like weaning, transportation, commingling, etc. The purpose of this project was to test if externally observed physical characteristics of calves on arrival at a stocker facility can be used to predict calfhood vaccination status determined based on antibody titer levels. Knowledge of highly correlated characteristics could allow stocker operators to reduce the occurrence of BRD through targeted management strategies, thus lowering morbidity, mortality, and treatment costs. Ear notches, blood, and visual characteristics were collected …
Comparison Of The Humoral Immune Response Following Both Bacterial Challenge And Rnai Of Major Factors On Proliferation Of Bartonella Quintana In The Human Louse, Jake Zina
Masters Theses
Human body lice, Pediculus humanus humanus, and head lice, Pediculus humanus capitis, have been hematophagous ectoparasites of humans for thousands of years. Despite being ecotypes, only body lice are known to transmit bacterial diseases to humans, and it appears that lower humoral and cellular immune responses allow body lice to possess a higher vector competence. We previously observed that the transcription level of the defensin 1 gene was up-regulated only in head lice following oral challenge of Bartonella quintana, a causative agent of trench fever, and also that body lice excreted more viable B. quintana in their …
Optimal Stocker Production Strategies For Spring And Fall Calving Cow Herds, Cora Beth Key
Optimal Stocker Production Strategies For Spring And Fall Calving Cow Herds, Cora Beth Key
Masters Theses
This thesis consists of two chapters discussing the economics of raising stocker beef cattle in the southeast United States. The objective of the first chapter is to estimate supplemental feed cost for spring-calving cows, spring-born stockers, cows calving in the fall, and fall-born stockers, while considering the seasonality of forage production and nutritional needs. The chapter explores ways producers can lower their feed costs and demonstrates the importance of managing hay expenses for cattle producers to control cost. The objective of chapter two is to determine the profit-maximizing stocker period in an integrated cow-calf and stocker operation with both fall- …
Efficacy Of Sequential Estrus Synchronization And Timed Artificial Insemination In Beef Cattle Beginning 19 Days After An Initial Timed Artificial Insemination, Courtnie Carter Bridges
Efficacy Of Sequential Estrus Synchronization And Timed Artificial Insemination In Beef Cattle Beginning 19 Days After An Initial Timed Artificial Insemination, Courtnie Carter Bridges
Masters Theses
A breeding program utilizing single fixed-timed artificial insemination (AI) was compared to a resynchronization program for a second fixed-timed AI. Angus and Angus cross primiparous and multiparous cows were randomly assigned to one of two treatment regimens; either single TAI (CON; n=468) or two sequential TAI (RS; n=473). The main objectives were assessing pregnancy rates and analyzing fetal age to estimate calving distribution rates between the two treatment groups. The 7-day CO-synch + CIDR protocol was utilized in both treatment groups to synchronize estrus for TAI-1 at 60h - 66h post- Prostaglandin injection (PGf2α). Animals in the RS treatment groups …
The Spatial Ecology And Habitat Selection Of A Spotted Turtle (Clemmys Guttata) Population In Southwest Michigan, Michela Coury
The Spatial Ecology And Habitat Selection Of A Spotted Turtle (Clemmys Guttata) Population In Southwest Michigan, Michela Coury
Masters Theses
Turtles are arguably the most threatened group of vertebrates on the planet. Anthropogenic influences such as habitat loss and fragmentation, road mortality, subsidized predators, climate change, and illegal collection have contributed to their global decline. Understanding which isolated populations of turtles are viable despite these synergistic threats is crucial for making conservation and management decisions. In Michigan, the spotted turtle (Clemmys guttata) is a threatened species subjected to many negative anthropogenic influences that accelerate its rapid population decline. In order to properly sustain their populations, the goal of my research was to investigate relevant factors that help us …
Effects Of Protein Concentration And Beta-Adrenergic Agonists On Ruminal Microbial Communities In Finishing Beef Heifers, Alison P. Pfau
Effects Of Protein Concentration And Beta-Adrenergic Agonists On Ruminal Microbial Communities In Finishing Beef Heifers, Alison P. Pfau
Masters Theses
To improve animal performance and modify growth by increasing lean tissue accretion, beef cattle production has relied on use of growth promoting technologies such as beta-adrenergic agonists. These synthetic catecholamines, combined with the variable inclusion of rumen degradable (RDP) and undegradable protein (RUP), may improve feed efficiency and rate of gain in finishing beef cattle. However, research about the impact of beta-adrenergic agonists and protein level and source on the ruminal microbiome is limited. The objective of this study was to determine the effect of different protein concentrations and beta-adrenergic agonist (ractopamine hydrochloride; RAC usage) on ruminal bacterial communities in …
Songbird-Mediated Insect Pest Control In Low Intensity New England Agriculture, Samuel J. Mayne
Songbird-Mediated Insect Pest Control In Low Intensity New England Agriculture, Samuel J. Mayne
Masters Theses
Global agricultural intensification has caused large-scale wildlife declines, but agricultural lands that maintain natural habitats can support healthy wildlife populations and receive significant ecosystem services from these natural communities. However, how on-farm biodiversity results in beneficial ecosystem services is highly variable and is reported to differ among taxa and guilds. One group that has attracted attention for their potential beneficial role in reducing pest abundance are birds. Understanding the role of bird communities and individual species in pest control could be important for managing farms under a low intensity agroecological framework. In New England, farmers are increasingly applying low intensity …
Differences In The Growth And Bioenergetics Between Centrarchids In A Midwestern Power Plant Lake, Kyle Joseph Rempe
Differences In The Growth And Bioenergetics Between Centrarchids In A Midwestern Power Plant Lake, Kyle Joseph Rempe
Masters Theses
Black Crappie Pomoxis nigromaculatus and Bluegill Lepomis macrochirus are popular sportfishes that co-occur in similar bodies of water throughout most of the Central and Eastern United States but have distinct thermal physiologies. L. macrochirus is often found in warmer regions of freshwater reservoirs, where P. nigromaculatus tends to exploit cooler regions, especially in power plant cooling reservoirs. The coal-fired Coffeen Power Station ceased energy production in October 2019, providing an ideal scenario to study the organismal effects of the sudden shift in thermal regime. Previous studies showed that P. nigromaculatus inhabited the less thermally-impacted areas of Coffeen Lake, whereas L. …
Comparison Of Bigheaded Carp Reproduction In Tributaries Of The Illinois And Wabash Rivers, David J. Yff
Comparison Of Bigheaded Carp Reproduction In Tributaries Of The Illinois And Wabash Rivers, David J. Yff
Masters Theses
Understanding environmental conditions that support bigheaded carp (Hypophthalmichthys sp.) reproduction in tributary rivers provides key predictions of their invasion throughout the United States. Past research, primarily in larger order rivers, identified factors such as discharge, temperature, and turbidity as main environmental drivers of bigheaded carp reproduction. I used the variable hydrologic conditions between the Illinois and Wabash Rivers to compare environmental conditions between tributaries of each basin, determine which conditions influence bigheaded carp reproduction in tributaries, and predict tributaries likely to support bigheaded carp reproduction. Three tributaries of the Illinois and Wabash Rivers were sampled for ichthyoplankton from …
The Effect Of Bovine Endometrial Cell Conditioned Media On In Vitro Produced Bovine Embryo Development And Gene Expression, Mary A. Oliver
The Effect Of Bovine Endometrial Cell Conditioned Media On In Vitro Produced Bovine Embryo Development And Gene Expression, Mary A. Oliver
Masters Theses
In vitro produced (IVP) bovine embryos provide enhanced genetic value and a means for studying early embryonic development. However, IVP embryos are developmentally compromised and are lower quality compared to in vivo derived (IVD) embryos. Consequently, after transfer pregnancy rates from IVP embryos are consistently lower, as they exhibit the highest amounts of pregnancy failure during the early embryonic phase (prior to day 28). Progesterone (P4) has been shown to influence bovine endometrial epithelial (EPI) and stromal fibroblast (SF) cells to secrete developmentally important molecules that promote early embryo development and conceptus elongation. However, IVP embryos are not exposed to …
Factors Influencing Primate Hair Microbiome Diversity, Catherine Kitrinos
Factors Influencing Primate Hair Microbiome Diversity, Catherine Kitrinos
Masters Theses
Primate hair is both a substrate upon which essential social interactions occur and an important host-pathogen interface. As commensal microbes provide important immune functions for their hosts, understanding the microbial diversity in primate hair could provide insight into primate immunity and disease transmission. While studies of human hair and skin microbiomes show differences in microbial communities across body regions, little is known about the nonhuman primate hair microbiome. In this study, we collected hair samples (n=159) from 8 body regions across 12 nonhuman primate species housed at 3 US institutions to examine 1) the diversity and composition of the primate …
Assessing The Short-Term Effects Of Translocation On Freshwater Mussels: Is Habitat Or Water Quality More Important?, Joshua D. Arnold
Assessing The Short-Term Effects Of Translocation On Freshwater Mussels: Is Habitat Or Water Quality More Important?, Joshua D. Arnold
Masters Theses
Freshwater mussels (Order: Unionida) are very important to the function of aquatic ecosystems and are typically indicators of good water quality. They provide a valuable link between the water column and the benthic substrate in which they live and are a valuable food resource for many species of animals. However, most species native to North America are currently threatened with extinction, to the point that more than 70% of native freshwater mussels are listed as either threatened or endangered at the state or federal level. The cause of this decline can be attributed to historical over exploitation, habitat alteration, and …
Nutritional Effects On Scrotal Circumference, Motility And Morphology Of Spermatozoa And Cytokine Concentrations Within Seminal Plasma Of Beef Cattle, Taylor D. Harrison
Nutritional Effects On Scrotal Circumference, Motility And Morphology Of Spermatozoa And Cytokine Concentrations Within Seminal Plasma Of Beef Cattle, Taylor D. Harrison
Masters Theses
Sire fertility is impacted by a wide variety of environmental and management conditions, potentially causing critical losses within beef operations. Nutrition is a main influence on the ejaculate due to bulls undergoing multiple planes of nutrition yearly. By identifying the effects of nutritional planes on the ejaculate composition, reproductive efficiency could be maximized at pregnancy establishment. We hypothesized periods of differing nutritional planes and body condition score (BCS) would affect bull fertility and cytokine concentrations within the ejaculate of bulls. Mature Angus bulls (n = 11) were individually housed and randomly assigned to one of two treatments: 1) …
Defining The Role Of Rbbp4 In Oocyte Maturation And Preimplantation Development Using Trim-Away, Holly L. Barletta
Defining The Role Of Rbbp4 In Oocyte Maturation And Preimplantation Development Using Trim-Away, Holly L. Barletta
Masters Theses
Retinoblastoma-binding protein 4 (RBBP4) is a subunit of chromatin remodeling factor 1 (CAF-1) and is essential for mammalian oocyte maturation, embryo survival, and embryo implantation. RBBP4 also localizes to the chromatin and is a ubiquitously expressed nuclear protein. Previous methods used to study this protein include short interfacing RNAs (siRNAs) and CRISPR/Cas9. These techniques have limitations such as determining an indirect depletion of proteins, may trigger compensatory mechanisms, and may not be useful in non-dividing primary cells. A new, acute, and rapid endogenous protein depletion technique called Trim-Away, can overcome these limitations. Trim-Away is also widely applicable since it can …
Life History, Diet And Habitat Of The Federally Endangered Laurel Dace (Chrosomus Saylori), Shawna M. Fix
Life History, Diet And Habitat Of The Federally Endangered Laurel Dace (Chrosomus Saylori), Shawna M. Fix
Masters Theses
Laurel Dace (Chrosomus saylori) is a small, freshwater minnow that is endemic to headwater streams on Walden Ridge, Tennessee. The species was listed as federally endangered in 2011 and the U.S. Fish and Wildlife Service Recovery Plan indicates a need for life history, diet and habitat studies. A literature review, samples of archived specimens, and a surrogate species, the Tennessee Dace (Chrosomus tennesseensis), were used to describe the life history and diet of the Laurel Dace. A total of 370 Tennessee Dace were collected from Laurel Ford Branch, a stream 8.6 km from the Laurel Dace …
Evaluation Of Environmental Factors Influencing American Marten Distribution And Density In New Hampshire, Donovan Drummey
Evaluation Of Environmental Factors Influencing American Marten Distribution And Density In New Hampshire, Donovan Drummey
Masters Theses
Though the American marten (Martes americana) is widely distributed across northern North America, habitat use and population abundance vary widely across the range. Due to its status as a furbearer, the species has been extensively researched, resulting in a large body of knowledge about the species’ ecology, distribution, and abundance, as well as drivers of population structure and dynamics. More recently, marten research has shifted focus to genetics, habitat associations, and estimation of population state variables. The rapid increase in estimation of states such as occupancy, abundance, and density has likely been driven by the increasing accessibility of …
Evaluation Of Lake Sturgeon (Acipenser Fulvescens) Spawning Success On An Artificial Reef Constructed In The Kalamazoo River, Michigan, Jason P. Lorenz
Evaluation Of Lake Sturgeon (Acipenser Fulvescens) Spawning Success On An Artificial Reef Constructed In The Kalamazoo River, Michigan, Jason P. Lorenz
Masters Theses
Since lake sturgeon Acipenser fulvescens monitoring began on the Kalamazoo River in 2009, there has been no documentation of survival past the egg stage. Spawning has occurred directly below Calkins Dam and, in 2016, an artificial spawning reef was constructed 275-m downstream to improve habitat and increase survival to the larval stage. The site was monitored with egg mats and larval drift nets to document evidence of successful natural reproduction of lake sturgeon one year prior to construction (2016) and three years post-construction of the artificial reef (2017-2019). Fertilized lake sturgeon eggs were captured all four years of the study …
Fecal Findings: Investigating Eastern Massasauga Rattlesnake Diet Using Dna Metabarcoding, Alyssa Swinehart
Fecal Findings: Investigating Eastern Massasauga Rattlesnake Diet Using Dna Metabarcoding, Alyssa Swinehart
Masters Theses
Characterizing the diet of imperiled species using minimally invasive methods is crucial to understanding their conservation requirements. DNA metabarcoding methods have been used to characterize the diet primarily in mammalian systems, while reptiles are heavily underrepresented in this literature. Here, we apply a DNA metabarcoding approach to study the diet of the eastern massasauga rattlesnake (Sistrurus catenatus); a Federally Threatened snake found throughout the Great Lakes Region. Eighty-three fecal samples collected across 10 different massasauga populations located in Michigan were sequenced. We use universal metazoan primers and develop a host-specific oligonucleotide blocker to uncover the full potential diet …
Spatial And Demographic Patterns Of Two Threatened Turtle Species In An Urban Environment, Carly E. Brouwers
Spatial And Demographic Patterns Of Two Threatened Turtle Species In An Urban Environment, Carly E. Brouwers
Masters Theses
Urban development is a global threat to native wildlife. The process of urbanization reduces and degrades the useable habitat of a region, and creates novel “urban ecosystems” that possess new threats and stressors to local species. Turtles are one of the most threatened vertebrate groups worldwide, and are particularly at risk of decline in urban ecosystems due to reduced nesting success, increased road mortality events, altered movement patterns, and increased predation rates. Eastern box and Blanding’s turtles are two at-risk turtle species in the state of Michigan, USA, primarily due to land use change. Presently, there are urban populations of …