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Microvascular Endothelial Cells Of The Bovine Corpus Luteum: A Comparative Study Of The Estrous Cycle And Pregnancy, Jessica A. Cherry
Microvascular Endothelial Cells Of The Bovine Corpus Luteum: A Comparative Study Of The Estrous Cycle And Pregnancy, Jessica A. Cherry
Master's Theses and Capstones
Endothelial cells derived from the corpus luteum (CLENDOs) exhibit a diverse array of characteristics that seemingly complement their wide-ranging properties in luteal function and fate. Reproductive status of the animal (i.e., non-pregnant vs. pregnant) may contribute to the phenotypic/physiologic diversity of microvascular endothelial cells, but information in this regard is limited. Here, structural and functional attributes of CLENDOs derived from the estrous cycle (Days 9-12) were compared with those of pregnancy (Day 60). Initially, lectin binding properties were examined in the cells. Using fluorescent-tagged lectins for Bandeiraea simplicifolia I or concanavalin A, flow cytometric analysis indicated that lectin-binding on CLENDOs …
The Expression Of Heat Shock Protein 70, Heat Shock Protein 90 And Heat Shock Factor 1 Within The Bovine Midcycle Corpus Luteum After Stimulation With Temperature In Vitro And Pgf2alpha In Vitro And In Vivo, Melissa N. Kopka
Master's Theses and Capstones
Environmental and biological stressors affect cellular function. Heat shock proteins (HSPs) are molecular chaperones that safeguard cells against these stressors by protecting endogenous proteins from degradation. Heat shock factors (HSF) are their transcriptional regulators. In the present study, we evaluated whether heat (Experiment 1) or prostaglandin F2alpha (PGF2alpha; Experiments 2 and 3) induces the expression of HSP 70, HSP 90 and HSF1 in the bovine corpus luteum (CL). In summary, in vitro heat treatment elicited a later response in luteal cells by increasing HSP 70 expression at 24 hrs, while in vivo PGF2alpha treatment leads to an earlier response by …
Temporal Expression Of Protein Mediators During Pgf2alpha-Induced Luteolysis In Sheep, Bryon F. Ricketts
Temporal Expression Of Protein Mediators During Pgf2alpha-Induced Luteolysis In Sheep, Bryon F. Ricketts
Master's Theses and Capstones
To study dynamic in vivo biochemical changes within the corpus luteum (CL) during luteolysis, a physiological model mimicking the onset of natural luteolysis was established, in which sheep received sequential systemic pulses of PGF2alpha (20ug/min/lhr) at mid-luteal phase of the estrous cycle. We previously demonstrated that after one pulse of PGF2alpha, the protein levels of extracellular matrix (ECM) regulators, tissue inhibitors of metalloproteinases (TIMPs) -1 and -2 decreased dramatically within one hour of PGF2alpha infusion, while matrix metalloproteinase (MMP)-2 activity increased 4 hrs post infusion. In summary, the early and dramatic decrease in TIMP-1 and TIMP-2 proteins, accompanied by an …
Effects Of Stz Induced Hyperglycemia And Hyperlipemia On Ppargamma And Abca1 In A Hamster Model Of Atherogenesis, Craig Lamarca
Effects Of Stz Induced Hyperglycemia And Hyperlipemia On Ppargamma And Abca1 In A Hamster Model Of Atherogenesis, Craig Lamarca
Master's Theses and Capstones
Diabetes mellitus accelerates atherogenesis, but the exact mechanisms involved are unknown. This study examined the effects of hyperglycemia on the inflammatory transcription factor peroxisome proliferator activated receptor gamma (PPARgamma), known to be involved in atherogenesis, and the ATP binding cassette A1 (ABCA1) protein that is a downstream product of PPARgamma. ABCA1 is a membrane bound transporter that is involved in reverse cholesterol transport via high density lipoprotein cholesterol (HDL-C). Hamsters were divided into two groups; hyperlipemic animals fed a high fat/high cholesterol control diet (C) and hyperlipemic/hyperglycemic animals that received the high fat diet and were administered streptozotocin. Half of …