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Expression And Purification Of Snake Antivenom Peptide In Pichia Pastoris, Israel Juarez Contreras
Expression And Purification Of Snake Antivenom Peptide In Pichia Pastoris, Israel Juarez Contreras
Master's Theses
It has been shown that a peptide of the first 10-15 N-terminal amino acids of the lethal toxin neutralizing factor (LTNF) protein found in opossums (Didelphis spp.) holds promise as a low-cost therapy for snake envenomation. To date, the 11-mer has been expressed in E. coli and shown to neutralize snake venoms. However, possible endotoxin concerns warrant an investigation into other microbial hosts. The methylotrophic yeast, Pichia pastoris, shows promise as an alternative host. Active LTNF peptide was expressed and purified in P. pastoris. This was accomplished by subcloning a tandem repeat of the first 15 N-terminal amino acids of …
The Deformability Of Red Blood Cells In Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (Me/Cfs), Brendan Robert Schmidt
The Deformability Of Red Blood Cells In Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (Me/Cfs), Brendan Robert Schmidt
Master's Theses
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a disease that causes profound fatigue in its sufferers as well as loss of concentration, headaches, and sleep abnormalities. Unfortunately, the disease is poorly diagnosed, and etiology is not known. Although the ME/CFS patients do not show anemic readings, and their arterial partial pressure is normal, it is possible that their extreme post-exertional malaise stems from poor microvascular perfusion and oxygenation. Previous studies have indicated that the red blood cells (RBCs) in ME/CFS patients show evidence of oxidative damage. We hypothesized that the RBC deformability metrics can serve as a reliable biomarker of ME/CFS …
High-Throughput Screening Of A Metagenomic Library Using A Novel E. Coli Chip For Discovery Of Antibiotic Resistance Genes, Richard Paul Manarin Tiongco
High-Throughput Screening Of A Metagenomic Library Using A Novel E. Coli Chip For Discovery Of Antibiotic Resistance Genes, Richard Paul Manarin Tiongco
Master's Theses
The growing prevalence of antibiotic resistance is one of the greatest challenges facing humankind today. The Centers for Disease Control and Prevention (CDC) have estimated that about 2 million people in the US each year develop an infection caused by antibiotic-resistant bacteria, and about 23,000 people die. To combat this growing crisis, efforts geared toward the discovery of novel compounds, such as antimicrobial peptides (AMPs), have spurred. However, current approaches for identification of such compounds have proven to be costly, time-consuming and ineffective. This problem underscores the need for a new approach, one that is fundamentally different from traditional methods …