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Design And Synthesis Of N-Succinyl-L,L-2.6-Diaminopimelic Acid Desuccinylase Inhibitors As Potential Novel Antibiotics, Thomas Dipuma
Design And Synthesis Of N-Succinyl-L,L-2.6-Diaminopimelic Acid Desuccinylase Inhibitors As Potential Novel Antibiotics, Thomas Dipuma
Master's Theses
N-Succinyl-L,L-2,6-Diaminopimelic Acid Desuccinylase (DapE) is a bacterial enzyme located in the lysine biosynthetic pathway of all Gram-negative and most Gram-positive species of bacteria including the notorious ESKAPE pathogens, or pathogenic strains of bacteria that have developed significant levels of resistance to currently available antibiotics. As a means to combat this challenge of growing antimicrobial resistance, we have identified the bacterial enzyme DapE as a conserved, novel enzymate target within these resistant bacterial strains,as DapE is responsible for the production of m-DAP and lysine which are ultimately employed in bacterial cell wall synthesis. The overall aim of this research is to …
Child Soldiers & Juveniles In Gangs: Opposite Sides Of The Same Coin With Dissimilar Legal Liabilities In International And American Constitutional Law, Jason Blevins
Master's Theses
No abstract provided.
Characterizing The Dynamic Localization Of Cmi In Early Drosophila Development, Asra Habibullah
Characterizing The Dynamic Localization Of Cmi In Early Drosophila Development, Asra Habibullah
Master's Theses
The COMPASS-like family of lysine methyltransferases, MLR/MLX complexes, are epigenetic regulators that are essential for normal development through the methylation of the fourth lysine residue on histone 3 (H3K4), a universal epigenetic mark associated with active transcription. This family of complexes is highly conserved from yeast to mammals and the genes encoding the human MLR complexes have been associated with various developmental diseases and cancers (Dingwall and Fagan, 2019). In D. melanogaster, the enzymatic methyltransferase core of this complex is composed of two proteins: Cara Mitad (Cmi, also known as Lpt) and Trithorax-related (Trr). Although these proteins have been shown …
Investigating The Viral Nature Of Neuronal Capsid Genes, Michael Manuel Long
Investigating The Viral Nature Of Neuronal Capsid Genes, Michael Manuel Long
Master's Theses
Memory formation is an essential part of everyday life. Neuronal communication is thought to be a key driver behind memory formation. There are two heavily studied methods of neuronal communication: chemical (neurotransmitter-mediated) and electrical (physically attached). When issues arise environmentally or genetically, people can experience one of many devastating memory disorders i.e. Alzheimer’s disease and other Dementias. Due to their prevalence, the treatments for disorders that hinder memory formation are a high priority for pharmaceutical companies. Currently, many of the treatments available focus on neurotransmitters or neuronal activity, and scientists are far from a cure. However, there may be an …
The Jewish Dancing Body: Body Image In Jewish Folk Dance, Alexa Berkowitz
The Jewish Dancing Body: Body Image In Jewish Folk Dance, Alexa Berkowitz
Master's Theses
Throughout my life as a dancer, I often wondered if body image issues were purely found in Western concert dance (such as ballet and modern dance) and if folk dance instilled a sense of ownership over one’s body. It was apparent to me that being asked to stare at yourself in the mirror for hours on end in a ballet class, or any other dance style, forced you to see every tiny defect in your body. For me personally, this caused a lot of body dysmorphia and body image issues that I carry to this day. While some folk dance …
Parental Autonomy Support And Children’S Stem Engagement During An At-Home Tinkering Activity, Bianca Martins Aldrich
Parental Autonomy Support And Children’S Stem Engagement During An At-Home Tinkering Activity, Bianca Martins Aldrich
Master's Theses
Parents play an important role guiding children’s learning of science, technology, engineering, and mathematics (STEM) in informal educational contexts. In this project, we considered the ways in which parents use autonomy supportive or controlling language to encourage or discourage children's independence in problem solving, as well as their feedback to children’s ideas and behaviors. We looked at the association between autonomy support and children’s behavioral, STEM, story, and emotional engagement during an at-home tinkering and storytelling activity. Parents and their 4- to 10- year old children were observed at home via Zoom. We coded parents’ and children’s behaviors using a …
Murine Tbk1 Is Required By Flt3+ Leukemia Stem Cells Yet Dispensable In Homeostatic Hematopoiesis, Austin Runde
Murine Tbk1 Is Required By Flt3+ Leukemia Stem Cells Yet Dispensable In Homeostatic Hematopoiesis, Austin Runde
Master's Theses
The TANK-binding kinase 1 (TBK1) is a cytoplasmic, serine/threonine kinase and a critical activator of the innate immune system. Reports have implicated TBK1 as a promoter of multiple cancer types. Acute myeloid leukemia (AML) is a cancer arising from the hematopoietic stem/progenitor cells (HSPCs). Although ~70% of AML patients achieve complete remission with chemotherapy, at least half will relapse due to the failure to eradicate leukemia stem cells (LSCs). As our previous data indicate the TLR-TAK1/TICAM-1 axes regulate LSCs, we suspect TBK1 also regulates LSCs. To study TBK1 in AML, we generated tamoxifen-inducible, Tbk1-knockout (Tbk1NULL) mice and Tbk1NULL MLL-AF9+ mouse …
Targeting The Subacute Progression Of Hippocampal Cellular Senescence Following Repetitive Mild Traumatic Brain Injury, Jacob Edward Exline
Targeting The Subacute Progression Of Hippocampal Cellular Senescence Following Repetitive Mild Traumatic Brain Injury, Jacob Edward Exline
Master's Theses
Repetitive mild traumatic brain injury (rmTBI) increases an individual’s risk for developing persistent deficits in recognition memory with symptoms experienced by up to 30% of rmTBI patients. Cellular senescence has been suggested as an underlying mechanism of persistent rmTBI symptoms; however, the subacute progression of cellular senescence following rmTBI has not been evaluated. Furthermore, the use of subacute testosterone administration to treat persistent recognition memory deficits and the cellular and molecular underpinnings has yet to be well characterized. Our lab utilizes a 5-hit closed-head rmTBI rat model to induce deficits in recognition memory and determine the effects of subacute testosterone …
Effect Of Selenof Loss In Combination With A Western Diet On Tumorigenesis, Brenna Joy Flowers
Effect Of Selenof Loss In Combination With A Western Diet On Tumorigenesis, Brenna Joy Flowers
Master's Theses
SELENOF is an understudied selenium-containing protein that has previously been postulated to behave as a tumor suppressor in the breast. Examination of patient databases showed that SELENOF levels were lowest in tumors from patients with aggressive late-stage breast cancers. Whether loss of SELENOF drives breast tumorigenesis remains to be determined. To address this question, we used juvenile female wild type or systemic Selenof knockout mice and exposed them to 7,12-Dimethylbenz[a]anthracene (DMBA), a carcinogen that replicates the multistep process of breast tumorigenesis. Previous reports have shown that loss of Selenof led to glucose and metabolic dysregulation in mice suggesting a link …
Abortion, Medieval Christianity, And The Christian Far-Right: The Harms Of Rhetoric And Misconstruing The Past, Madison Givens
Abortion, Medieval Christianity, And The Christian Far-Right: The Harms Of Rhetoric And Misconstruing The Past, Madison Givens
Master's Theses
This research explores the intersection of abortion, Medieval Christianity, and the Christian far-right, with a focus on the harms of rhetoric and the misconstruing of history. The study argues that a selective reading of history is being used by the Christian far-right to justify violence against reproductive rights activists, including police brutality and mass shootings, as well as to promote forced sterilization and other forms of reproductive control. This thesis examines the harms of rhetoric and misconstruing the past in the abortion and medieval Christianity debate. By analyzing the role of language, symbols, and history in shaping the abortion debate …
Decolonizing A Settler Colonial State: How Canada And The United States Have Approached Investigations Into Indigenous Boarding And Residential Schools, Holly Jacobs
Master's Theses
Indigenous boarding and residential schools were in use for over 100 years in both Canada and the United States. Grown from numerous policies aimed at assimilation, dispossession, and genocide of the Indigenous peoples of North America, these residential schools had a particular aim: to wipe away all cultural and familial ties of every individual and to replace them with beliefs and values of white settlers. Though the schools are no longer in operation, their legacy of erasure and pain continues to affect survivors, their descendants, and Indigenous communities across both nations. This thesis looks at what the federal governments of …
Physical Activity, Self-Determination, And Self-Conscious Emotions In Adolescents And Young Adults With And Without Autism Symptomatology, Dakota Morales
Physical Activity, Self-Determination, And Self-Conscious Emotions In Adolescents And Young Adults With And Without Autism Symptomatology, Dakota Morales
Master's Theses
Previous literature suggests that autistic individuals engage in physical activity less frequently compared to typically developing peers (Bandini et al., 2013; Jones et al., 2017). This is noteworthy because exercise interventions for autistic individuals have found that engaging in physical activity can improve motor, social, and behavioral skills (Anderson-Hanley et al., 2011; Duffy et al., 2017; Nicholson et al., 2011; Oriel et al., 2011). Thus, the present study seeks to understand what factors may promote physical activity engagement, such as self-determination motivation regulations and body-related self-conscious emotions, in adolescents and young adults with and without autism symptomatology. Participants included 51 …
Engagement With Ethnic Practices: How Ethnic Communities Contribute To Second-Generation Asian American Assimilation, Bảo-Trân T. Nguyễn
Engagement With Ethnic Practices: How Ethnic Communities Contribute To Second-Generation Asian American Assimilation, Bảo-Trân T. Nguyễn
Master's Theses
Previous research on spatial assimilation has described ethnic enclaves as places withmany recently arrived immigrants and fewer socioeconomic resources. As immigrants become more assimilated, they move to more affluent neighborhoods in proximity to Anglos. However, recent studies on resurgent ethnicity challenge the idea of the spatial assimilation by suggesting that Asian immigrants and subsequent generations continue to live near co-ethnics, despite gaining socioeconomic status. The transition from traditional ethnic enclaves to resurgent ethnic communities or ‘ethnoburbs’ indicate shifting understandings of what ethnic communities mean to Asian Americans. Although, Asian Americans are, on average, attaining higher socioeconomic status, the emergent importance …
How To Build A Cortex: Coordinated Assembly Of Cortical Septins And Actomyosin In The Leader Bleb, Asia Marie Paguntalan
How To Build A Cortex: Coordinated Assembly Of Cortical Septins And Actomyosin In The Leader Bleb, Asia Marie Paguntalan
Master's Theses
In confined spaces, migrating cells can undergo mesenchymal-to-amoeboid transitions by altering their cortical dynamics and adhesion with the environment. Septins frequently associate with cortical actin and non-muscle myosin (NMII), but the functional nature of these interactions remains unclear. Upon non-adhesive confinement and NMII enrichment, fibroblasts can switch to a fast, leader bleb-based mode of motility, characterized by the absence of adhesions and stress fibers and formation of a single, elongated leader bleb. During this transition, cortical actin remodeling and polarized NMII contractility drive leader bleb stabilization by generating long-range cortical flows, in coordination with changes in septin localization and assembly …
Development Of Inattention And Executive Dysfunction In Youth With Spina Bifida: Condition Severity Variables As Predictors, Allison D. Payne
Development Of Inattention And Executive Dysfunction In Youth With Spina Bifida: Condition Severity Variables As Predictors, Allison D. Payne
Master's Theses
Spina bifida (SB) is associated with neurologic impairments that increase individuals’ risk for neuropsychological deficits, particularly inattention and executive dysfunction. While extant literature has yet to examine the development of inattention in youth with SB, some research suggests that these youth may not experience the age-related improvements in executive dysfunction seen in the general population. SB is a heterogeneous condition; thus, it is important to consider variability in condition severity when examining outcomes over time in youth with SB. Lesion level and shunt status are commonly used as indicators of SB severity and have been significantly associated with variability in …
Building Strength Versus Getting Lean: An Analysis Of The Gendered Nature Of Fitness In Crossfit, Barre, And Personal Training, Margaret M. Jones
Building Strength Versus Getting Lean: An Analysis Of The Gendered Nature Of Fitness In Crossfit, Barre, And Personal Training, Margaret M. Jones
Master's Theses
Like many industries in contemporary society, the fitness sector is heavily gendered, andthus needs to be examined as a sector that both creates and reinforces gendered bodies. Building on previous single-method studies, I utilized a multi-methods approach of ethnography and interviews to analyze organizational and individual experiences with gender and fitness. Using West and Zimmerman’s concept of “doing gender,” I analyzed fitness organizations as a setting where gender and inequality are actively reproduced. By analyzing three fitness organizations with different gendered audiences (a barre studio, a CrossFit gym, and a personal training facility) I looked at how gender and fitness …
Changing Vaccine Hesitant Attitudes Of Parents Using Moral Persuasion, Max Vitro
Changing Vaccine Hesitant Attitudes Of Parents Using Moral Persuasion, Max Vitro
Master's Theses
In the fight against Covid-19, overt, science-based messaging is not enough to persuade everyone to get vaccinated no matter how encouraging the data. Recent studies on attitudes toward vaccines and other health-promoting measures have provided clues as to why so many are still opposed, suggesting many who were resistant had reasons that were rooted along moral grounds. This process of moralization occurs when a belief becomes a moral matter of ‘right and wrong’ rather than a means to an end. Because moral beliefs are more entrenched, they’ve proven much more difficult to change. There is one approach that could increase …
Murine Tbk1 Is Required By Flt3+ Leukemia Stem Cells Yet Dispensable In Homeostatic Hematopoiesis, Austin Patrick Runde
Murine Tbk1 Is Required By Flt3+ Leukemia Stem Cells Yet Dispensable In Homeostatic Hematopoiesis, Austin Patrick Runde
Master's Theses
The TANK-binding kinase 1 (TBK1) is a cytoplasmic, serine/threonine kinase and a critical activator of the innate immune system. Reports have implicated TBK1 as a promoter of multiple cancer types. Acute myeloid leukemia (AML) is a cancer arising from the hematopoietic stem/progenitor cells (HSPCs). Although ~70% of AML patients achieve complete remission with chemotherapy, at least half will relapse due to the failure to eradicate leukemia stem cells (LSCs). As our previous data indicate the TLR-TAK1/TICAM-1 axes regulate LSCs, we suspect TBK1 also regulates LSCs. To study TBK1 in AML, we generated tamoxifen-inducible, Tbk1-knockout (Tbk1NULL) mice and Tbk1NULL MLL-AF9+ mouse …
Incorporating Sex Chromosomes In Transcriptome Prediction Models And Improving Cross-Population Prediction Performance, Daniel S. Araujo
Incorporating Sex Chromosomes In Transcriptome Prediction Models And Improving Cross-Population Prediction Performance, Daniel S. Araujo
Master's Theses
Transcriptome prediction models built with data from European-descent individuals are less accurate when applied to different populations because of differences in linkage disequilibrium patterns and allele frequencies. We hypothesized multivariate adaptive shrinkage may improve cross-population transcriptome prediction, as it leverages effect size estimates across different conditions - in this case, different populations. To test this hypothesis, we made transcriptome prediction models for use in transcriptome-wide association studies (TWAS) using different methods (Elastic Net, Matrix eQTL and Multivariate Adaptive Shrinkage in R (MASHR)) and tested their out-of-sample transcriptome prediction accuracy in population-matched and cross-population scenarios. Additionally, to evaluate model applicability in …
Characterization Of The Full-Length Bag3 Protein And Stress Induced Formation Of Bag3-Z, Ahmed Gamal Abdalla Zied
Characterization Of The Full-Length Bag3 Protein And Stress Induced Formation Of Bag3-Z, Ahmed Gamal Abdalla Zied
Master's Theses
Bcl2-associated athanogene-3 (BAG3) is a pro-autophagy co-chaperone that we havepreviously shown localizes to the cardiac sarcomere and is critical for proteostasis and maintenance of normal sarcomeric function. Indeed, BAG3 loss in heart failure (HF) results in accumulation of ubiquitinated sarcomeric proteins, and depressed maximum force generating capacity (Fmax). However, how BAG3 is regulated in the cell is not well understood, with uncertainty about its structure and proteoforms. During our analysis of human heart tissue, BAG3 appears as a “doublet”, with one band at 74 kDa (BAG3-Z) and a second at a higher 85 kDa (BAG3-FL). Previous studies hypothesized the full-length …
A Population Level Examination Of The Incredible Fruit Diversity In Astragalus Lentiginosus, Quinn Thomas
A Population Level Examination Of The Incredible Fruit Diversity In Astragalus Lentiginosus, Quinn Thomas
Master's Theses
Astragalus lentiginosus is the most taxomically diverse species in the North American flora with over 40 recognized varieties. The incredible diversity within A. lentiginosus, particularly in respect to its fruit morphology, makes it an exceptional model to study adaptation and evolution. However, it remains unknown whether fruit morphology is an adaptation and, if so, the extent to which selection or neutral evolutionary processes shape the striking diversity in this trait. In order to understand the adaptive significance of fruit morphology we must first understand the genetic structure of A. lentiginosus. Varieties of A. lentiginosus vary considerably in their range size …
Comparison Of Taxonomy Assignment And Strain Detection In Urobiome Communities Using 16s Rrna Sequencing And Shotgun Metagenomic Sequencing, Delaney Sauer
Master's Theses
Advancements in sequencing technologies have enabled scientists to gain insight into the microbes that inhabit the human body, including the urinary tract. Cataloging the bacteria that inhabit the urinary tract has primarily relied on amplification and sequencing of specific variable regions of the 16S rRNA gene enabling genus-level taxonomic identification. Recently, shotgun metagenomic sequencing has been employed such that bacterial taxonomy as well as the functionality that they encode can be inferred. In this study, we compare taxonomies assigned by 16S sequencing and shotgun metagenomic sequencing of the urinary microbiota (urobiome) of females with and without a clinical diagnosis of …
The Effect Of Intrafamilial Racism On Biracial Identity Conflict, Lauren Tan
The Effect Of Intrafamilial Racism On Biracial Identity Conflict, Lauren Tan
Master's Theses
This study aimed to describe the relation between experiences of intrafamilial racism (i.e., racism perpetrated by family members against other family members), caregiver support, and identity conflict in biracial individuals. Identity conflict is defined as perceived incompatibility with two or more aspects of one’s identity (Sarno et. al., 2015) and is an experience likely to be common among biracial individuals. Regression analyses were conducted to determine the relation between intrafamilial racism, caregiver support, and identity conflict. Moderation analyses were performed to determine the impact of intrafamilial racism on identity conflict at different levels of caregiver support. We hypothesized that intrafamilial …
Identification Of New Substrates From Pseudomonas Aeruginosa Exos, Adam V. Thota
Identification Of New Substrates From Pseudomonas Aeruginosa Exos, Adam V. Thota
Master's Theses
Pseudomonas aeruginosa is a gram-negative environmental bacterium that uses its intrinsic and acquired antibiotic resistance to infect patients with compromised epithelium, such as cystic fibrosis and burn wounds, and corneal keratitis. In order to cause disease, most strains of P. aeruginosa use the type three secretion system (T3SS) to disrupt cellular signaling and integrity. However, P. aeruginosa may also secrete exotoxins after invading host cells, which prolongs host cell death and maintains an intracellular niche. Of the T3 secreted effector toxins, ExoS, specifically the ADP-ribosyltransferase activity of ExoS, was shown to delay the death of invaded corneal cells, but it …
Teachers’ Perceptions Of Ells By Racial Identity: A Pilot Study, Ha Tran
Teachers’ Perceptions Of Ells By Racial Identity: A Pilot Study, Ha Tran
Master's Theses
ABSTRACTAs racial and political tensions grow within the United States, it is not uncommon for negative teacher perceptions of English Language Learners (ELLs) to be documented in educational research. This observed phenomenon is especially worrisome for three reasons: (1) teachers’ perceptions of their students are known to affect their pedagogy, (2) teacher perception and pedagogy influences student outcomes, and (3) the ELL population continues to be the quickest growing subset within the American public-school student body. While extensive, previous research has not attempted to compare teacher perceptions of ELLs across varying racial identity groups of ELLs. Thus, this pilot study …
Ecclesiological Sovereignty: Toward An Understanding Of The Sovereignty Of The Pope In The Context Of Luther's Responses, Erik Lee Grayvold
Ecclesiological Sovereignty: Toward An Understanding Of The Sovereignty Of The Pope In The Context Of Luther's Responses, Erik Lee Grayvold
Master's Theses
This thesis seeks to address the contention between the perceived sovereign power of the Papacy and the Protestant Reformers. It will first provide a historical survey of how the Pope came to understand himself through the history of the Church. It will then analyze responses from the Reformers regarding this topic, focusing mainly on confessional documents and responses to these documents from the Roman Catholic Church. It will conclude with a survey of Luther’s specific responses to the situation and how his response to Papal authority and sovereignty impacted this theological development. This survey will also acknowledge situations in which …
A Comparative Study Of The Impact Of Regimes In Latin America’S Education Systems: The Cases Of Mercosur, Alba-Tcp, And Pa, Victoria Desimoni
A Comparative Study Of The Impact Of Regimes In Latin America’S Education Systems: The Cases Of Mercosur, Alba-Tcp, And Pa, Victoria Desimoni
Master's Theses
This thesis presents a comparative study of the regional education policies and programs in three Latin American (LA) regional entities (hereafter ‘regimes’ and ‘regionalisms’): The Southern Common Market (MERCOSUR), the Bolivarian Alliance for the Peoples of Our America – People’s Trade Treaty (ALBA-TCP), and Pacific Alliance (PA). Each of these represents different regionalisms coexisting in LA (hegemonic, post-hegemonic, and counter-hegemonic regionalisms), which makes them ideal for comparative analysis. This project aims to compare these very diverse regimes to interrogate the similarities and dissimilarities, as well as discover who (the regional or the global) is shaping education systems in this area …
Bicycling To Level The Field: A Study Of Divvy In Chicago, Bushra Ghaniwala
Bicycling To Level The Field: A Study Of Divvy In Chicago, Bushra Ghaniwala
Master's Theses
With the onset of COVID-19, norms across the world shifted, including the way people moved in major cities. In order to conduct a comparative analysis, understanding transportation habits before COVID-19 hit cities is important. In this paper, I have focused on Divvy bikes in the city of Chicago, which are touted as a means to achieving first- and last-mile transit especially in underserved communities. I am interested in initiating the line of inquiring into who Divvy bikes served during a time when there was major fear around high transmission of COVID-19 on trains and buses due to the close proximity. …
Constructing Planetary Citizens Through Ecopedagogy In U.S. Social Studies Standards, Madeline Anne Rainey
Constructing Planetary Citizens Through Ecopedagogy In U.S. Social Studies Standards, Madeline Anne Rainey
Master's Theses
The world is in the age of the Anthropocene, where humans are impacting the environment to disastrous effects. The capitalist economy, promoting neoliberal policies of mass consumption, has exacerbated the world's environmental deterioration and social inequity. The rights and responsibilities people hold have been rapidly changing with the fourth industrial revolution. Globalization and Information and Communications Technology (ICT) have further expanded notions of citizenship. While there have been numerous attempts to bring the environment into schools, it has not emphasized what this crisis deserves. Ecopedagogy, as a critical theory, explicitly examines the interplay between environmental and social problems and challenges …
How Chicago Public Schools Engaged African American Parents In The Closing Of Austin Career Academy High School, Shani Blackwell
How Chicago Public Schools Engaged African American Parents In The Closing Of Austin Career Academy High School, Shani Blackwell
Master's Theses
The role of African American parents in education reform initiatives in Chicago is not clearly defined. Local School Councils (LSC) allow parents to make school related decisions in Chicago. But LSCs are removed from decisions about whether or not to place school on or off probation, and whether or not to close schools based on performance or building utilization. In 2013, African American and Latinx parents expressed outrage over the decision by Chicago Public Schools (CPS) to close 49 schools with protests, including a hunger strike by a group of parents, and pleas to the Chicago Board of Education (BOE). …