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Vitrification Of Immature And Mature Bovine Oocytes, Paige T. Hardin
Vitrification Of Immature And Mature Bovine Oocytes, Paige T. Hardin
LSU Master's Theses
Vitrification is the latest technique used in cryopreservation, the ability to utilize this method with oocytes and embryos has become a valuable system. Vitrification has been successful with bovine embryos and oocytes but is far from optimal. Following cryopreservation storage discarding embryos can cause ethical issues, and mature oocytes have fragile organelles that can be detrimentally affected by ice crystal formation during freezing. Immature oocytes have not formed some of these temperature sensitive microfilaments and can circumvent these detrimental effects. The common intracellular cryoprotective agents are dimethyl sulfoxide, glycerol and ethylene glycol. Different combination of these agents have been reported …
3-D Printed Conveyor For Continuous-Mode Cryopreservation Of Individual Samples, Melissa Kaye Eskridge
3-D Printed Conveyor For Continuous-Mode Cryopreservation Of Individual Samples, Melissa Kaye Eskridge
LSU Master's Theses
Lack of standardization of terminology and protocols, combined with limited access to freezing equipment due to cost constraints and lack of portability, has created a need for inexpensive cryopreservation devices. Distribution of standardized methods of freezing cells would enable the development and adoption of standards on materials and protocols. Some cryopreservation methods are limited by the amount of cells available and the toxicity of certain cryopreservation agents. These studies would benefit from the ability to cool individual samples. All commercially available and proposed cooling devices operate by cooling batches of samples at the same time. The cryopreservation conveyor was designed …