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LSU Master's Theses

2016

Bioremediation

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Biodegradation Of Buried Mc252 Oil In Coastal Beach Sands By Pah Degraders In Response To Oxygen Biostimulation, Leeann Renee Fitch Jan 2016

Biodegradation Of Buried Mc252 Oil In Coastal Beach Sands By Pah Degraders In Response To Oxygen Biostimulation, Leeann Renee Fitch

LSU Master's Theses

Six years following the Deepwater Horizon oil spill, buried MC252 oil persists on a beach segment in Fourchon, LA due to the natural anaerobic conditions of tidal groundwater. A field trial of in situ aerobic bioremediation of buried oil began on July 2015. Oxygen was added to the subsurface using Waterloo emitters which deliver O2 through diffusion via pressurized tubing in fixed groundwater wells. The multi-well injection system provided sufficient oxygen concentration in the groundwater in the immediate area of the emitters, resulting in a shift in the composition of the diverse, halophilic, hydrocarbon-degrading microbial population. Stable isotopic and radiocarbon …


Spatial Biodegradation Of Mc252 Crude Oil On A Coastal Headland Beach, Zachary Joseph Romaine Jan 2016

Spatial Biodegradation Of Mc252 Crude Oil On A Coastal Headland Beach, Zachary Joseph Romaine

LSU Master's Theses

Following the Deepwater Horizon oil spill in April, 2010, hard structures were constructed to deter oil migration into sensitive marsh areas behind Fourchon Beach, LA These hard structures created conditions for accumulation and burial of oil across an 8 foot deep beach vertical profile. Buried oil persists in these areas due to anaerobic conditions of beach groundwater. The objectives of this thesis are to compare the rate and extent of biodegradation of 3-ring PAHs in crude oil deposits from the surface and subsurface, and to investigate effects on beach groundwater after introduction of O2. Field samples were removed from 2011-2016 …