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Optimization Of The Expression Of Surface Antigen Sag1/2 Of Toxoplasma Gondii In The Yeast Pichia Pastoris, Init Ithoi
Optimization Of The Expression Of Surface Antigen Sag1/2 Of Toxoplasma Gondii In The Yeast Pichia Pastoris, Init Ithoi
Init Ithoi
Surface antigens are the most abundant proteins found on the surface of the parasite Toxoplasma gondii. Surface antigen 1 (SAG1) and Surface antigen 2 (SAG2) remain the most important and extensively studied surface proteins. These antigens have been identified to play a role in host cell invasion, immune modulation, virulence attenuation. Recombinant SAG1/2 was cloned and expressed in yeast Pichia pastoris. We describe here optimization of critical parameters involved in high yield expression of the recombinant SAG1/2. Our results suggest that recombinant SAG1/2 were best expressed at 30 masculineC, pH 6 and 1% methanol as the carbon source by X33 …