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Induction Of Aryl Hydrocarbon Hydroxylase And Ethoxyresorufin O-Deethylase In Human And Murine Cells By 2,3,7,8-Tetrachlorodibenzo-P-Dioxin (Tcdd) And Polycholrinated Biphenyls (Pcbs)., Andreas. Vamvakas
Electronic Theses and Dissertations
To assess the relevance of H4IIE for human populations, we examined levels of aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin O-deethylase (EROD) activities H4IIE rat liver, HepG2 human liver and MCF-7 human breast cells after exposure to a variety of HAHs. These included TCDD, and three coplanar non-ortho substituted polychlorinated biphenyl (PCB) congeners: 3,3$\sp\prime$,4,4$\sp\prime$-tetrachlorobiphenyl (PCB 77), 3,3$\sp\prime$,4,4$\sp\prime$5-pentachlorobiphenyl (PCB 126), and 3,3$\sp\prime$,4,4$\sp\prime$,5,5$\sp\prime$-hexachlorobiphenyl (PCB 169), either alone or in combination. AHH and EROD activities were also examined after treatment with muscle tissue extracts of Lake Ontario rainbow trout (Oncorhynchus mykiss). H4IIE was generally more sensitive to all HAH treatments. AHH and EROD activities …
Two-Dimensional Nuclear Magnetic Resonance Studies Of Thymosin-Alpha(1) (A Myosin Light Chain Kinase Activating Peptide), And Ribonuclease A In The Presence Of Uridine Vanadate., Timothy Daniel. Veenstra
Two-Dimensional Nuclear Magnetic Resonance Studies Of Thymosin-Alpha(1) (A Myosin Light Chain Kinase Activating Peptide), And Ribonuclease A In The Presence Of Uridine Vanadate., Timothy Daniel. Veenstra
Electronic Theses and Dissertations
1. The structure of the peptide thymosin $\alpha\sb1$ has been investigated by circular dichroism and one- and two-dimensional nuclear magnetic resonance spectroscopy. Thymosin $\alpha\sb1$ is a highly acidic peptide composed of 28 amino acid residues. Through the use of circular dichroism and two-dimensional nuclear magnetic resonance techniques, the structure of thymosin $\alpha\sb1$ in 30% (v/v) deuterated 2,2,2-trifluoroethanol has been solved. Thymosin $\alpha\sb1$ contains an $\alpha$-helix extending from residue 16 to 26 and a turn between residues 5 and 8. Thymosin $\alpha\sb1$ has been shown to be a potent activator of skeletal muscle myosin light chain kinase, a well known calmodulin-dependent …
Purification, Characterization, And Functional Investigations Of Novel Platelet Proteins., Jeffrey Stephen. Baldwin
Purification, Characterization, And Functional Investigations Of Novel Platelet Proteins., Jeffrey Stephen. Baldwin
Electronic Theses and Dissertations
Recently, a novel Kunitz inhibitor of trypsin-like enzymes has been discovered in bovine platelets that has a higher degree of association for plasmin than trypsin, and thereby has been termed bovine platelet plasmin inhibitor (BPPI). Basic problems in the purification, resulting in protein that was essentially insoluble, has hampered structural determinations by x-ray or NMR analysis. An investigation into the cause of the protein insolubility has revealed that an excessively high concentration of acetonitrile, used to elute the protein from a C18 reversed-phase cartridge, was most probably the cause of the insolubility. Concentrated BPPI solutions permitted the preparation of a …
Some Aspects Of Glutathione And L-Arginine/Nitric Oxide Metabolism In The Maintenance Of Platelet Function., Daniel Joseph. Sexton
Some Aspects Of Glutathione And L-Arginine/Nitric Oxide Metabolism In The Maintenance Of Platelet Function., Daniel Joseph. Sexton
Electronic Theses and Dissertations
Evidence is presented here for the regulation of platelet activation by intra-platelet levels of glutathione (GSH) and L-arginine. Our data is consistent with the intra-platelet GSH ((GSH) $\sb{\rm ip}$) modulation through the regulation of the thromboxane A$\sb2$ (TxA$\sb2$) biosynthesis. The reduction of (GSH) $\sb{\rm ip}$ by 100 $\mu$M 1-chloro-2,4-dinitrobenzene (CDNB) enhanced thrombin-induced platelet aggregation and ADP-induced platelet aggregation was inhibited by the elevation of (GSH) $\sb{\rm ip}$ through a facilitative GSH-specific transport system. Platelet facilitative GSH uptake was subsequently characterized as being Na$\sp+$-independent, concentration dependent (K$\sb{\rm M}$ and V$\sb{\rm max}$ for GSH uptake in platelet plasma membrane vesicles (PPMV) is …
In Vitro Short-Term Human Bioassays In Environmental Cytotoxicity And Genotoxicity Assessments., Masumeh. Abdolalipour
In Vitro Short-Term Human Bioassays In Environmental Cytotoxicity And Genotoxicity Assessments., Masumeh. Abdolalipour
Electronic Theses and Dissertations
In this study, an in vitro human cell culture, the HepG2 cell line, has been adapted and calibrated to be used as a surrogate human model system in environmental toxicology studies. To evaluate both cellular and genetic effects of test chemicals, a cytotoxicity test and a genotoxicity test were developed in the HepG2 cell line. A Neutral Red (NR) dye-uptake assay was used to assess cytotoxicity, and an alkaline unwinding, DNA break assay (AU) was developed to detect genotoxicity of both known toxicants as well as unknown environmental samples. The response of the NR and AU bioassays was calibrated by …