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Study Of The Effects Of Chronic Cadmium Exposure On The Pathogenesis Of Pulmonary Arterial Hypertension., Dakotah Dominique Cathey
Study Of The Effects Of Chronic Cadmium Exposure On The Pathogenesis Of Pulmonary Arterial Hypertension., Dakotah Dominique Cathey
Electronic Theses and Dissertations
We reported pulmonary artery hypertension (PAH) patients had elevated levels of Cd in both blood and urine, therefore, this study tested whether Cd directly induces PAH or facilitates PAH pathogenesis in mouse models. C57/6J mice were initially exposed to drinking water with or without 5ppm Cd for 8 weeks. Then, half the mice in both control and Cd groups were given SU5416 and hypoxia (SuHx) for 4 weeks to induce PAH, resulting in 4 subgroups: Control, Cd, PAH, and Cd+PAH. Diastolic and systolic functions of the left and right ventricles (LV, RV) were examined with echocardiography before and after PAH. …
Scalable Solution Processing Of Niox Nanoparticles., Peter James Armstrong
Scalable Solution Processing Of Niox Nanoparticles., Peter James Armstrong
Electronic Theses and Dissertations
Perovskite solar cells (PSCs) are a promising alternative to silicon-based photovoltaics. However, PSCs face several challenges due to shortcomings in their stability, module efficiency, and scaled production. Although PSCs is still a young field of research, significant attention has been given to demonstrating power conversion efficiencies that are on par with traditional silicon. With that target reached, converting the laboratory demonstration into practical materials to increase access and abundance of solar energy are among the next large targets for the field. This comes with material challenges for perovskite and their companion charge transport layers (CTLs). Among the charge transport materials …
Adaptive Personalized Drug Delivery Method For Warfarin And Anemia Management: Modeling And Control., Affan Affan
Adaptive Personalized Drug Delivery Method For Warfarin And Anemia Management: Modeling And Control., Affan Affan
Electronic Theses and Dissertations
Personalized precision medicine aims to develop the appropriate treatments for suitable patients at the right time to obtain optimal results. Personalized medicine is challenging due to inter- and intra-patient variability, narrow therapeutic window, the effect of other medications, comorbidity (more than one disease at a time), nonlinear patient dynamics, and time-varying patient dose response characteristics which include bleeding (internal and external). This research aims to develop a framework for an adaptive personalized modeling and control method with minimum clinical patient specific dose response data for optimal drug dosing. The proposed methodology is applied to anemia and warfarin management. It is …
Investigating The Effects Of Subchronic Dietary Zinc Supplementation On Diet-Induced Metabolic Dysfunction-Associated Steatotic Liver Disease., Oluwanifemi Esther Bolatimi
Investigating The Effects Of Subchronic Dietary Zinc Supplementation On Diet-Induced Metabolic Dysfunction-Associated Steatotic Liver Disease., Oluwanifemi Esther Bolatimi
Electronic Theses and Dissertations
Zinc deficiency is associated with metabolic dysfunction-associated steatotic liver disease (MASLD). Previous studies show zinc supplementation improves steatosis, but its therapeutic effects in established MASLD remain unclear. We developed a model to characterize the effects of zinc supplementation on high-fat diet (HFD) induced MASLD and hypothesized established MASLD would be attenuated. Mice were fed control diet or HFD for 12 weeks and then grouped into normal or zinc-supplemented diets for 8 additional weeks. At euthanasia, plasma and liver tissues were collected for phenotypic analysis. Twelve weeks of HFD altered glucose clearance and body composition. Eight weeks of subsequent zinc supplementation …
Communicating In Crisis: Rhetorical (De)Stabilization During The Covid-19 Pandemic., Brittany Nicole Smart
Communicating In Crisis: Rhetorical (De)Stabilization During The Covid-19 Pandemic., Brittany Nicole Smart
Electronic Theses and Dissertations
This project explores the role of rhetoric in crisis—how rhetoric can contribute to both the stabilization and destabilization of a worldwide health emergency. Specifically, I utilize the COVID-19 pandemic as a case study to investigate how institutional rhetorics exacerbated the ongoing burnout epidemic amongst healthcare workers. Through a feminist, materialist take on institutional ethnography (Fullagar & Pavlidis, 2021; Griffith & Smith, 2014), I show how, while institutions like the CDC were under pressure to contain the spread of the virus, in the chaos of communicating safety regulations to healthcare professionals, they inadvertently subverted clinician autonomy and expertise by “coordinating” (LaFrance, …
Genetic Risk Scores For Hypertension And Chronic Kidney Disease In African Americans., Aastha Kakar
Genetic Risk Scores For Hypertension And Chronic Kidney Disease In African Americans., Aastha Kakar
Electronic Theses and Dissertations
Hypertension (HT) and chronic kidney diseases (CKD) are complex conditions, disproportionally prevalent among African Americans (AA) and are influenced by multiple genetic as well as environmental factors. Genetic risk scores (GRS) enable the analysis of the combined effects of multiple single nucleotide polymorphisms (SNPs). This study investigates the association of blood pressure (BP) and CKD GRS with BP, hypertension (HT) and CKD; early-onset HT and CKD; and investigates interactions of the GRSs with environmental measures in AA. We observed a significant association between the BP-GRS with HT, SBP and DBP. The odds of HT for one standard deviation (s.d.) increase …
Educator Perceptions Of The Care And Instruction Of Trauma Exposed Students In A Small, Urban, Christian School., Amanda L. Petters
Educator Perceptions Of The Care And Instruction Of Trauma Exposed Students In A Small, Urban, Christian School., Amanda L. Petters
Electronic Theses and Dissertations
Students of color in low socio-economic, high crime urban areas are exposed to a higher level of trauma than their White contemporaries. The trauma that they bring into school with them create challenges for their teachers working to eliminate barrier to students’ education. Widespread efforts have been attempted to thwart the social injustices that many students of color experience in schools including disproportionality around implementation of school rules and the equitable access to curriculum. I used Critical Race Theory to frame my study around students of color in urban areas experiencing disproportionate levels of trauma, and their teachers’ level of …
Bayesian Strategies For Propensity Score Estimation In Causal Inference., Uthpala I. Wanigasekara
Bayesian Strategies For Propensity Score Estimation In Causal Inference., Uthpala I. Wanigasekara
Electronic Theses and Dissertations
Causal inference is a method used in various fields to draw causal conclusions based on data. It involves using assumptions, study designs, and estimation strategies to minimize the impact of confounding variables. Propensity scores are used to estimate outcome effects, through matching methods, stratification, weighting methods, and the Covariate Balancing Propensity Score method. However, they can be sensitive to estimation techniques and can lead to unstable findings. Researchers have proposed integrating weighing with regression adjustment in parametric models to improve causal inference validity. The first project focuses on Bayesian joint and two-stage methods for propensity score analysis. Propensity score modeling …
Design, Fabrication, And Integration Of Robotic Skin Sensors For Human Robot Interaction., Olalekan Olakitan Olowo
Design, Fabrication, And Integration Of Robotic Skin Sensors For Human Robot Interaction., Olalekan Olakitan Olowo
Electronic Theses and Dissertations
Enhancing physical human-robot interaction in modern robotics relies on refining the tactile perception of robot skin sensors. This research focuses on crucial aspects of the development process, including fabrication techniques, miniaturization, and integration for a more efficient collaborative human-robot interface. The fabrication process of robot skin sensors, designed to mimic human skin, is explored both within and outside cleanroom environments. An enhanced technique is presented to increase fabrication yield and create more miniaturized sensor designs with feature sizes in the tens of microns. These sensors function as piezoresistive arrays using organic polymers like PEDOT: PSS as the pressure-sensing medium. Various …
Computational Investigations Of Bond Breaking Processes Using Dft And Td-Dft Approaches., Saurav Parmar
Computational Investigations Of Bond Breaking Processes Using Dft And Td-Dft Approaches., Saurav Parmar
Electronic Theses and Dissertations
The efficient application of DFT and TD-DFT has been harnessed to study bond-breaking processes in some molecules which play a prominent role in enzymatic reactions. The first application includes Radical S-adenosyl methionine (SAM) enzymes which are fundamentally important sources of organic radicals to initiate diverse radical reactions. Recently a bio-organometallic intermediate (Ω) that contains an Fe‒C bond has been characterized and shown to be a common feature of radical SAM enzymes. The strength of Fe‒C bond in Ω has been computed using broken-symmetry density functional theory (BS‒DFT). Additionally, Fe‒C bond dissociation energy (BDE) in Ω has been compared to that …
The Interplay Between Lung Adenocarcinoma, Nutrient Availability, And The Microbiome., Alexis A. Vega
The Interplay Between Lung Adenocarcinoma, Nutrient Availability, And The Microbiome., Alexis A. Vega
Electronic Theses and Dissertations
Lung cancer is one of the most common forms of cancer diagnosed, and has the highest lethality rate across all other forms of cancer in the U.S. While current therapeutic options include chemo-, immuno-, and radio-therapy, the benefits of caloric or nutrient restriction on cancer cells has also been investigated. Due to the many biological functions associated with methionine, many have proposed a methionine restricted diet would lead to favorable outcomes when combating cancer. Although our cells are incapable of synthesizing methionine, the bacteria found in our microbiome can. Furthermore, recent discoveries suggest an independent microbiome found within tumors that …
Electrodeposition Studies Of Gold Nanoparticles And Alginate Hydrogel Films., Harikrishnan Nambiathan Nambiar
Electrodeposition Studies Of Gold Nanoparticles And Alginate Hydrogel Films., Harikrishnan Nambiathan Nambiar
Electronic Theses and Dissertations
This dissertation investigates the electrochemical deposition (ECD) and electrophoretic deposition (EPD) processes involving citrate (cit)-stabilized Au nanoparticles (NPs) and calcium alginate (Ca-Alg-Au NP) hydrogel films. The first part of the dissertation investigates the influence of NP size, surface area (SA) coverage, and ligand stabilizers on the electrochemical growth of Au on Au NPs, aiming to enhance our understanding of the growth process during the nucleation and growth of metal NPs. The growth kinetics depend on Au NP size (4 nm > 15 nm > 50 nm) for a constant SA coverage, as determined by the overpotentials for ECD. The growth rate increases …
Investigating Mems Devices In Flow Conditions Relevant To Flow-Through Systems., Mohammad Shafquatul Islam
Investigating Mems Devices In Flow Conditions Relevant To Flow-Through Systems., Mohammad Shafquatul Islam
Electronic Theses and Dissertations
Advancements in microscale actuating technologies has substantially expanded the possibilities of interacting with the surrounding environment. Microstructures that deflect in response to mechanical forces are one of the largest application areas of microelectromechanical systems (MEMS). MEMS devices, functioning as sensors, actuators, and support structures, find applications in inertial sensors, pressure sensors, chemical sensors, and robotics, among others. Driven by the critical role of catalytic membrane reactors, this dissertation aims to evaluate enzyme activity on polymeric membranes and explore how fabrication methods from the field of Electrical and Computer Engineering (ECE) can incorporate sensing and actuation into these porous surfaces. Toward …
Causal Inference For The Effect Of Continuous Treatment On Time-To-Event Outcomes And Mediation Analysis On Health Disparities In Observational Studies., Triparna Poddar
Causal Inference For The Effect Of Continuous Treatment On Time-To-Event Outcomes And Mediation Analysis On Health Disparities In Observational Studies., Triparna Poddar
Electronic Theses and Dissertations
The dissertation comprises two projects related to causal inference based on observational data. In healthcare research, where abundant observational data such as claims data and electronic records are available, researchers often aim to study the treatment effect and the pathway of that effect. However, estimating treatment effects in observational data presents challenges due to confounding factors. The first project focuses on estimating continuous treatment effects for survival outcomes, while the second concentrates on mediation analysis, allowing the exploration of the pathway of the causal effect. Both projects involve addressing confounding variables. In the first project, I investigate estimation of the …
Quantifying Impacts Of Climate Change On Headwater Streamflow Regime In Robinson Forest: Insights From 35-Years Of Data Collection., Lauren Brown
Electronic Theses and Dissertations
Climate change may shift patterns of streamflow permanence in headwater systems by altering the frequency, magnitude, duration, timing, and rate of change of surface streamflow, impacting both local ecosystems as well as regional water budgets and availability. While much uncertainty surrounds modeling-based methods to quantify the impacts of climate change on water budgets, long-term hydrologic data collected from headwaters in experimental research forests serve as critical evidence to reduce such uncertainty. The objective of this study is to quantify shifts in frequency, magnitude, duration, timing, and rate of change of streamflow in two headwater catchments with relatively little recent disturbance …
Ethanol Dysregulates Sirt1-Mediated Lipogenic Signaling Pathways Through Α4*-Nicotinic Acetylcholine Receptors In Hepatocytes., Caitlin Christina Wilkerson
Ethanol Dysregulates Sirt1-Mediated Lipogenic Signaling Pathways Through Α4*-Nicotinic Acetylcholine Receptors In Hepatocytes., Caitlin Christina Wilkerson
Electronic Theses and Dissertations
Alcohol-associated liver disease (ALD) is a broad spectrum of diseases ranging from steatosis to severe hepatic cirrhosis, none of which have any FDA approved therapies. Downregulation of hepatic Sirtuin 1 (Sirt1) activity by ethanol has been shown to lead to an upregulation of the pro-lipogenic gene targets under its jurisdiction, resulting in increased lipogenesis and triglyceride accumulation. It was hypothesized that ethanol acts on α4*-nicotinic acetylcholine receptors (α4*-nAChRs) in hepatocytes to decrease Sirt1 expression, which ultimately dysregulates lipid metabolic homeostasis. Ethanol’s effects were investigated in cultured AML12 hepatocytes and in WT primary mouse hepatocytes acutely exposed to ethanol. Consistent with …
Connections Between Polyvictimization, Daily Stress, Emotion Regulation, And Mental Health Symptoms: An Ecological Momentary Assessment Study., Zoe L. Bridges-Curry
Connections Between Polyvictimization, Daily Stress, Emotion Regulation, And Mental Health Symptoms: An Ecological Momentary Assessment Study., Zoe L. Bridges-Curry
Electronic Theses and Dissertations
Background and Objectives: Individuals who experience polyvictimization, or victimization across multiple life contexts, evidence particularly severe outcomes across a range of mental health diagnoses. Preliminary evidence suggests that emotion regulation difficulties and stress sensitization effects may help explain observed links between trauma exposure and mental health symptoms. However, the use of between-subjects designs to address within-subjects processes – a common approach across this literature – can result in erroneous interpretation of findings. Moreover, widespread reliance on retrospective trait measures of emotion regulation, daily stressors, and mental health symptoms have limited insight into the ways that these processes unfold in daily …
Environmental Exposures And Aging., Daniel Chris Gomes
Environmental Exposures And Aging., Daniel Chris Gomes
Electronic Theses and Dissertations
In recent years, research into air pollution has shown that exposure to certain components in air pollution, primarily PM2.5 can accelerate biological aging and thereby lead to increased susceptibility to multiple diseases. We hypothesize that prolonged exposure to air pollutants can result in premature aging leading to extensive tissue dysfunction and susceptibility to diseases. To examine this, we exposed mice to PM2.5 for 9, 15, and 21 days, then measured the telomere lengths, cellular senescence, and histone methylation patterns of multiple cell types. We found consistently increased telomere attrition, cellular senescence and advanced age-consistent histone methylation patterns in groups exposed …
Genetically Engineering Gingipains For High Yield Large Scale Purification., Andrew E. Fuchs
Genetically Engineering Gingipains For High Yield Large Scale Purification., Andrew E. Fuchs
Electronic Theses and Dissertations
Porphyromonas gingivalis is a keystone oral pathogen in the progression of periodontal disease and has been implicated in the etiology of many other systemic human diseases. P. gingivalis produces primary virulence factors termed the gingipains, a group of cysteine proteases. There is a high demand for high purity gingipains for downstream in vitro studies where P.g. is implicated, both oral and systemic. Gingipain production candidate mutant P.g. strains were generated to isolate each gingipain for large scale purification and the purified gingipains characterized. Two mutants were determined not to be viable for large scale purification of gingipains, each for different …
A Phenomenological Study Of The Perceptions Of Inclusive Preschool Education Through The Lens Of Interdisciplinary Early Childhood Educators., Brooke Noon
Electronic Theses and Dissertations
Young children with special needs have been educated in early childhood settings with typically developing peers for over 40 years. Successful educational inclusion in the least restrictive environment requires appropriate aids and supports (Individuals with Disabilities Act, 2004). Although researchers have studied many aspects of special education, little investigation has been conducted on the perspectives of dual certified interdisciplinary early childhood educator’s (IECE) perceptions on full inclusion in preschool. This study was designed to examine the connection between the perceptions of IECE teacher’s experiences as a dual certified educator in preschool. In total, 10 IECE teachers were interviewed and completed …
Assessment Literacy Influenced By Administrator Identity And Professional Learning Confident Administrators Within Kentucky Independent School Districts., Amy J. Harris
Electronic Theses and Dissertations
The purpose of this qualitative phenomenological study is to gain greater understanding surrounding how administrators in northern Kentucky Independent School Districts describe their own experiences with assessments (assessment literacy). In addition, this study sought to gain knowledge from administrator experiences which may or may not have contributed to their confidence as leaders of assessment literacy. The context of this study was with the 11 Independent School Districts in the Northern Kentucky region, specifically with school level administrators. The significance of this study is to inform teacher preparation programs and current administrators of how and why individual self-efficacy and professional learning …
Synergistic Strategies In Sinter-Based Material Extrusion (Mex) 3d Printing Of Copper: Process Development, Product Design, Predictive Maps And Models., Kameswara Pavan Kumar Ajjarapu
Synergistic Strategies In Sinter-Based Material Extrusion (Mex) 3d Printing Of Copper: Process Development, Product Design, Predictive Maps And Models., Kameswara Pavan Kumar Ajjarapu
Electronic Theses and Dissertations
3D printing pure copper with high electrical conductivity and exceptional density has long been challenging. While laser-based additive manufacturing technologies suffered due to copper's highly reflective nature towards laser beams, parts printed via binder-assisted technologies failed to reach over 90% IACS (International Annealed Copper Standard), electrical conductivity. Although promising techniques such as binder jetting, filament, and pellet-based 3D printing that can print copper exist, they however still face difficulties in achieving both high sintered densities and electrical conductivity values. This is due to a lack of comprehensive understanding of property evolution from green to sintered states and the strategies that …
Environmental Exposures And Male Reproductive Outcomes., Damilola Rukayat Owoade
Environmental Exposures And Male Reproductive Outcomes., Damilola Rukayat Owoade
Electronic Theses and Dissertations
Male sexual dysfunction comprises various conditions, including erectile dysfunction (ED) and ejaculatory dysfunction. Testosterone deficiency (TD), which has been linked to some of these sexual dysfunctions, is characterized by low production of testosterone by the testes. These male reproductive outcomes may develop due to exposure to various risk factors, including environmental exposures. These environmental exposures may include volatile organic compounds (VOCs) and tear gas. This dissertation aims to examine the effects of tear gas exposure and VOCs on male reproductive outcomes. The association between tear gas exposure and male reproductive outcomes was assessed using data obtained from an online anonymous …
“I Became Myself”: Exploring Cultural Learning Through Stories And Storytelling., Leah Halliday-Johnson
“I Became Myself”: Exploring Cultural Learning Through Stories And Storytelling., Leah Halliday-Johnson
Electronic Theses and Dissertations
Despite broad acknowledgement in education that culturally responsive teaching is essential to meeting learners’ needs, defining and operationalizing culturally responsive teaching remains a challenge. Along with being designed to meet the needs of students from diverse racial and cultural backgrounds who have traditionally been underserved, one commonly identified feature of culturally responsive teaching is building on students’ cultural assets to promote their learning. And while stories and storytelling are among the most common teaching tools, storytelling pedagogies tend to use stories to deliver content or to assess student learning. This dissertation explores collaborative story sharing as an approach to helping …
The Impact Of Environmental Pollutants On Alcohol-Associated Liver Disease Pathogenesis., Tyler Charles Gripshover
The Impact Of Environmental Pollutants On Alcohol-Associated Liver Disease Pathogenesis., Tyler Charles Gripshover
Electronic Theses and Dissertations
Steatotic liver disease is brought upon by differing etiologies and its global prevalence is expected to keep increasing over the next several decades. Particularly, alcohol-associated liver disease (ALD) manifests from excessive alcohol consumption where the liver retains lipids and progresses with metabolic dysfunction. Previous studies have demonstrated that environmental pollutants, polychlorinated biphenyls (PCBs) promote metabolic dysfunction associated steatotic liver disease (MASLD), in diet-induced obesity models. However, it is unknown if environmental pollutants may also promote ALD pathogenesis. Thus, this dissertation focuses on characterizing the effects of PCB126 in a rodent ALD model followed by multiple ‘Omics approaches to identify mechanisms …
Biocargos: Capture And Release Gels For Optimized Storage Of Biologics., Chinmay Shashank Potnis
Biocargos: Capture And Release Gels For Optimized Storage Of Biologics., Chinmay Shashank Potnis
Electronic Theses and Dissertations
No abstract provided.
Using A Toxic Aging Coin To Assess Hexavalent Chromium-Induced Neurotoxicity., Samuel Thomas Vielee
Using A Toxic Aging Coin To Assess Hexavalent Chromium-Induced Neurotoxicity., Samuel Thomas Vielee
Electronic Theses and Dissertations
We are facing an aging crisis, with 20% of the U.S. population projected to be geriatric (65+) by 2030 and live another 40+ years. Age-related diseases accompany a growing geriatric population, emphasizing the need to understand their etiology. Environmental pollutants compound this crisis by: 1) geriatrics are more susceptible, exacerbating age-related diseases and comorbidities, and 2) they accelerate biological aging, inducing age-related diseases at younger ages. We address this crisis using a ‘toxic aging coin’ approach; heads examines how age impacts toxicity, tails examines how chemicals accelerate aging. This thesis applies the heads side for Cr(VI)-induced neurotoxicity across ages. We …
Quantification Of Tumor Biophysical Heterogeneity Through Mechanical And Ultrastructural Analysis., Bradley James Mahaffey
Quantification Of Tumor Biophysical Heterogeneity Through Mechanical And Ultrastructural Analysis., Bradley James Mahaffey
Electronic Theses and Dissertations
Glioblastoma (GBM) is a highly invasive, aggressive brain cancer that carries a median survival of 15 months. This poor prognosis is due, in part, to its resistance to standard therapeutic intervention. Recent studies have demonstrated that tumor heterogeneity plays a critical role in facilitation therapy resistance by mediating tumor adaptation through microenvironmental cues. Efforts to describe these microenvironmental differences may aid in the development of strategies to combat resistance. GBM can be separated into two distinct regions – a core and a rim, which are thought to drive specific aspects of tumor evolution. The core is proliferative as evidenced by …
Formalizing The Faustian Bargain Within The Healthcare Domain: An End-Of-Life Approach., Rachel Appel
Formalizing The Faustian Bargain Within The Healthcare Domain: An End-Of-Life Approach., Rachel Appel
Electronic Theses and Dissertations
A Faustian Bargain refers to an individual making a “deal with the devil,” exchanging something moral or sacred for an unattainable earthly good. The Faustian Bargain has been used to describe a ubiquitous social dilemma inherent to human civilization: exchanging individual liberty for public goods (e.g., security) provided by societal leaders and governments (Ostrom, 1980). Research on Faustian Bargains often examines tradeoffs between outcome utility (i.e., value derived from the outcomes of a decision) and procedural utility (i.e., value derived from being involved in the decision process (e.g., Frey et al., 2004). Much of the research on Faustian Bargains has …
Automated Usability Evaluation Utilizing Log Files And Data Mining Techniques., Sima Shafaei
Automated Usability Evaluation Utilizing Log Files And Data Mining Techniques., Sima Shafaei
Electronic Theses and Dissertations
Usability evaluation is one of the essential aspects of software production. This evaluation should be done during the entire life cycle of a software application, from pre-production to production and post-production. However, the collection and evaluation of usability data can be a very challenging, time-consuming, and expensive task to be conducted manually, particularly for certain types of products and working conditions. These challenges may include the need to recruit participants fully engage and motivate them during evaluation, and factor in environmental conditions. Other challenges may include collecting data in real-world environments, especially when the users are geographically dispersed, minimizing evaluator …