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Articles 1 - 9 of 9
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Detection Of Mycotoxins Using Surface-Enhanced Raman Spectroscopy, Lourdes B. Martinez Rojas
Detection Of Mycotoxins Using Surface-Enhanced Raman Spectroscopy, Lourdes B. Martinez Rojas
Doctoral Dissertations
Mycotoxins are toxic metabolites produced by fungus that can be parasites or saprophytes of crops or livestock forage. Consumer demand for plant-based foods and interest in animal-based foods originating from animals fed plant-based feed has been on the rise. Therefore, monitoring mycotoxins occurring in the food supply is more critical than ever. The goal of this project is to improve surface-enhanced Raman spectroscopy’s (SERS) ability to identify and detect mycotoxins using label-free SERS substrates. Two simple approaches were designed to enhance the detection of mycotoxins produced by the Aspergillus and Penicillium genera, ochratoxin A and aflatoxin B1. Ochratoxin A was …
High Throughput Analysis To Study Emerging Pollutants And Nanoparticle Fate In Biological Systems, Xiaolong He
High Throughput Analysis To Study Emerging Pollutants And Nanoparticle Fate In Biological Systems, Xiaolong He
Doctoral Dissertations
”The increasing applications of emerging and fugitive contaminants (EFCs) and engineered nanoparticles (ENPs) attract significant research interest for their potential risks to human health and the environment. In order to assess the health risks of these emerging contaminants, rapid and reliable analytical methods to measure the concentrations and fates of these contaminants are imperative. This dissertation focuses on the developments of advanced analytical methods and their applications to study those emerging contaminants in crop plant and simulated gastric fluid (SGF). Three types of mass spectrometry based methodologies have been developed, one is freeze-thaw/centrifugation extraction followed by high performance liquid chromatography …
Enumeration, Isolation, And Detection Of Campylobacter Species From Food Stuffs, Fecal Samples, And River Water Of East Tennessee, Molly Albin West
Enumeration, Isolation, And Detection Of Campylobacter Species From Food Stuffs, Fecal Samples, And River Water Of East Tennessee, Molly Albin West
Doctoral Dissertations
Campylobacter species (spp.) are a leading cause of bacterial foodborne illness. These Gram-negative, microaerophilic microorganisms are found within the intestinal tracks of warm-blooded animals and in humans can cause a gastroenteritis, campylobacteriosis. Recent outbreaks are linked to raw milk, water, and puppies; however, these outbreak isolates have expressed antibiotic resistance, a main treatment line for campylobacteriosis. While Campylobacter spp. detection improves, cases are mainly sporadic and largely underreported. East Tennessee counties experience a higher prevalence of campylobacteriosis compared to other regions of the state based upon Tennessee Department of Health (TDH) surveillance. It is important for researchers to explore the …
Development Of Bacteriophage Based Detection Technique For Food Safety And Environment Monitoring, Ziyuan Wang
Development Of Bacteriophage Based Detection Technique For Food Safety And Environment Monitoring, Ziyuan Wang
Doctoral Dissertations
Foodborne or waterborne pathogens which are responsible for numerous worldwide outbreaks of disease have caused serious health problems and enormous economic cost. Development of new bacterial detection technologies with high sensitivity and specificity is of great importance for food safety and public health. This has highlighted the significance of developing rapid and effective techniques for pathogen screening. Thus we aim to explore a bacteriophage mediated detection system for foodborne or waterborne bacteria monitoring. Bacteriophage as a novel recognition probe has been applied in various biosensor by immobilizing phage particles on solid sensor surface. They possess high specificity for targeted microorganism …
Single Particle-Inductively Coupled Plasma-Mass Spectrometry Technology Development For Metallic Nanoparticle Characterization In Complex Matrices, Yongbo Dan
Doctoral Dissertations
"As the rapid growing of nanotechnology, the release of engineered nanoparticles (ENPs) into the environment is inevitable. After entering the real environment, ENPs tend to react with different components of the ecosystem (e.g. water, soil, air, plants) and make their characterization difficult. Analyzing ENPs in these complex matrices still remains as a grand challenge. ENPs characterization is normally the first step of risk assessment. Current analytical techniques have shown some limitations in revealing the unique characteristics of ENPs in complex matrices and reliable analytical techniques are in urgent need. Single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) is an emerging …
Layer-By-Layer Antimicrobial N-Halamine Polymer Coatings For Food Contact Materials, Luis J. Bastarrachea Gutierrez
Layer-By-Layer Antimicrobial N-Halamine Polymer Coatings For Food Contact Materials, Luis J. Bastarrachea Gutierrez
Doctoral Dissertations
Cross contamination during food processing represents a risk for public health and financial burden. Surface modification of food contact materials to render them antimicrobial can be effective against such risk. The objective of the present work was to develop antimicrobial coatings with the potential to be applied in a variety of food contact materials. The polymer coatings developed became antimicrobial by incorporation of a type of chlorinated compounds called N-halamines, capable of regenerating their antimicrobial activity. Two layer-by-layer (LbL) assembly surface modification procedures were followed. In the first procedure, bilayers of branched polyethyleneimine (PEI) and poly(acrylic acid) (PAA) were applied …
Development Of Capillary-Driven Microfludic Biosensors For Food Safety And Quality Assurance, Fei He
Development Of Capillary-Driven Microfludic Biosensors For Food Safety And Quality Assurance, Fei He
Doctoral Dissertations
Rapid detection technologies with high sensitivity and selectivity for pathogenic bacteria are critical in food safety and quality assurance. Traditional laboratory benchtop techniques (i.e. culture and colony counting) are time consuming and require complex sample handling. Microfluidics-based Lab-on-a-Chip (LOC) systems offer a detection alternative where all detection steps are on one portable miniaturized device. The miniaturization of rapid foodborne pathogen detection at a low cost is especially ideal for resource-limited settings or for field-use. The goal of this study was to develop a disposable miniaturized microfluidic device for on-site pathogen detection. Capillary-driven microfluidics have been introduced in this study. Compared …
Investigating The Potential Of Plant-Derived Antimicrobials And Probiotic Bacteria For Controlling Listeria Monocytogenes, Abhinav Upadhyay
Investigating The Potential Of Plant-Derived Antimicrobials And Probiotic Bacteria For Controlling Listeria Monocytogenes, Abhinav Upadhyay
Doctoral Dissertations
Listeria monocytogenes is a major foodborne pathogen resulting in multiple foodborne outbreaks in United States. In this dissertation, the efficacy of six plant-derived antimicrobials (PDAs), namely trans-cinnamaldehyde, carvacrol, thymol, eugenol, β-resorcylic acid and caprylic acid, and five probiotic bacteria, namely Bifidobacterium bifidum (NRRL-B41410), Lactobacillus reuteri (B-14172), L. fermentum (B-1840), L. plantarum (B-4496), and Lactococcus lactis subspecies lactis (B-633) was investigated for controlling L. monocytogenes. Specifically, the efficacy of aforementioned PDAs in reducing L. monocytogenes biofilms on abiotic surfaces, and pathogen populations on popular high-risk foods such as frankfurter and cantaloupe was investigated. In addition, the efficacy of PDAs and …
Intention To Comply With Food Safety Messages In A Crisis As A Function Of Message Source And Message Reliability, Karen June Freberg
Intention To Comply With Food Safety Messages In A Crisis As A Function Of Message Source And Message Reliability, Karen June Freberg
Doctoral Dissertations
A key role of public relations is to manage crises, unexpected yet unpredictable events that cause emotional and physical harm (Coombs, 2007). Among the challenges in handling a crisis effectively is dealing with the various media in which information is presented. Because the use of social media in a crisis is a relatively new phenomenon, further understanding of the challenges and opportunities of these media is warranted. Part of meeting this challenge requires precise modeling of consumer responses to safety messages. To remedy gaps in our understanding of social media and food safety crisis communications, consumer intent to comply with …