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Genetic Variation Of The Lyme Disease Spirochete, Borrelia Burgdorferi, And Its Tick Vector, Ixodes Dammini, Diane Amy Caporale
Genetic Variation Of The Lyme Disease Spirochete, Borrelia Burgdorferi, And Its Tick Vector, Ixodes Dammini, Diane Amy Caporale
Doctoral Dissertations
The causative agent of Lyme disease, Borrelia burgdorferi, is a spirochete transmitted among vertebrate hosts by Ixodid ticks. The outer surface of bacteria plays an important role in the ability to establish an infection in its host. The possibility that positive selection acts to accelerate the evolution of the outer surface protein genes was examined by comparing the DNA sequences of the OspA/B operon of B. burgdorferi isolates from North America and abroad. The Mean d$\rm\sb s$ values were two-fold higher than mean d$\rm\sb N$ values. Central regions of the OspB gene show an excess of silent mutations, while end …
The Detection Of Enteric Viruses By Nylon-Based And In Situ Nonisotopic Probe Assays And Their Use In Treated And Untreated Waters, Norman James Moore
The Detection Of Enteric Viruses By Nylon-Based And In Situ Nonisotopic Probe Assays And Their Use In Treated And Untreated Waters, Norman James Moore
Doctoral Dissertations
Potable water is a primary need of humankind. In the interest of public health, the water should be free of infectious agents, such as bacteria, intestinal parasites, and viruses. The current method for assaying water for viruses is cell culture, which is expensive, labor intensive, and time consuming. Nucleic acid probes offer an alternative method which is less costly, more rapid, and requires less training and experience. Consequently, this study provides alternatives to viral monitoring of water sources.
Three nonradioactive nucleic acid probe assays were developed and evaluated by colormetric and chemiluminescent signals. A biotin-tailed probe was able to detect …
A Functional Dissection Of The Yeast Ccr4 Protein And Identification Of Associated Factors, Michael Preston Draper
A Functional Dissection Of The Yeast Ccr4 Protein And Identification Of Associated Factors, Michael Preston Draper
Doctoral Dissertations
The yeast CCR4 protein is required for the expression of a number of genes involved in non-fermentative growth, including glucose repressible ADH2, and is the only known suppressor of mutations in the SPT6 and SPT10 genes, two genes which are believed to be involved in chromatin maintenance. It is shown here that CCR4 is able to activate transcription when fused to a heterologous DNA binding domain. The transcriptional activation ability of CCR4, in contrast to many other activators, was glucose regulated. Two activation domains were identified, one of which was glucose responsive and encompassed a glutamine-proline rich region similar to …