Digital Commons Network^™

Auxin-Induced Degradation Of Dream Proteins, Lin-9 And Lin-54, In Caenorhabditis Elegans, Karli E. Chosa

Dissertations, Master's Theses and Master's Reports

The Dp, Retinoblastoma, E2F, And MuvB (DREAM) complex mediates transcriptional repression and is highly conserved throughout a number of species, including vertebrates, Drosophila melanogaster, and Caenorhabditis elegans. Differing from mammalian DREAM, C.elegans DRM, appears to act solely in a repressive role, with the MuvB subcomplex (LIN-9, LIN-37, LIN-52, LIN-53, and LIN-54) playing a key role in the repression of genes. In this study, we use the auxin-inducible degron (AID) system, an effective, fast-acting, tool used in the degradation of degron-tagged proteins to individually deplete two key proteins of the MuvB subcomplex, LIN-9 and LIN-54, in C. elegans. The …

Recommendations For A Community-Led Multi-Stakeholder Watershed Management Plan In Rural Panama, Tristan Odekirk

Dissertations, Master's Theses and Master's Reports

Valle Risco is a small, rapidly growing rural town in northwest Panama. As the town grows and develops those who live there have expressed concern at sustainably managing the surrounding watershed. This report consists of some considerations and suggestions for the community of Valle Risco as they build a strong town that plays a central role in the socioeconomic development of the region. This information is based on the experience of the author while he served the town with the U.S. Peace Corps. Recommendations include solutions for wastewater management, storm water drainage, consistent water quality monitoring, and the use of …

Short-Term Fasting And Autonomic Control, Joshua Gonzalez

Dissertations, Master's Theses and Master's Reports

Obesity is a chronic metabolic disorder associated with increased risk of cardiovascular disease. Evidence suggests that chronic intermittent fasting improves cardiometabolic health and reduces arterial blood pressure. However, the mechanisms underlying the reductions in blood pressure and improved cardiovascular health observed from chronic fasting studies remain unclear. The autonomic nervous system has a central role in the regulation of blood pressure and is essential for cardiovascular homeostasis. We conducted a study to investigate how acute fasting influences autonomic control of blood pressure at rest and during stress. Twenty-five young, healthy, normal weight, normotensive participants were tested twice, once in the …

Hydrocarbon Biodegradation And Microbial Community Composition In Freshwater Systems And Enrichment Cultures, Emma Byrne

Dissertations, Master's Theses and Master's Reports

In this study, we investigated if significant differences existed seasonally in the microbial response to oil in the Straits of Mackinac, and if crude (Bakken) and refined (non-highway diesel) oil exposure had impacts on microbial community composition and hydrocarbon biodegradation across seasons using a microcosm-level experiment. Ambient microbial communities differed between seasons, with significantly enriched microbial groups present between all sample types except for between fall 23 ℃ and fall 4 ℃ microcosms. We found significantly different microbial communities between control samples and oil-amended samples in every season, but no significant community differences between either oil type. We found Amplicon …

Deformation Manifold Learning Model For Multi Walled Carbon Nanotubes, Shashank S. Pathrudkar

Dissertations, Master's Theses and Master's Reports

Two-Dimensional (2D) materials are being studied widely by researchers due to their superior material properties over the bulk materials. Since the isolation of graphene in 2004, graphene has gained popularity amongst the 2D materials community. Graphene when rolled into sheets form Carbon Nanotubes (CNTs) which possess excellent mechanical and electrical properties. Concentric stacks of CNTs yield Multi-walled Carbon Nanotubes (MWCNTs) which are superior to CNTs in certain aspects. It has been well established that the deformation of CNTs and MWCNTs change their mechanical and electrical properties significantly. This has opened doors for CNTs into numerous applications and also piqued the …

Transient Maneuvers And Pressure Analysis On An Automotive Torque Converter, Abigail A. Hempy

Dissertations, Master's Theses and Master's Reports

This work involves an investigation of internal fluid behavior of an automotive torque converter in a front-wheel drive automatic 6-speed transmission. The fluid behavior is identified through 29 pressure transducers instrumented throughout the torus, acquired through IR Telemetrics multiplexed channels, and analyzed with custom MATLAB code. The main vehicle operating conditions studied in this research are back drive scenarios or low motoring torques. Findings showed speed and torque limits for open converter coast down conditions as well as pressure field behavior with negative input torque.

Base Vibration Effects On Additive Manufactured Part Quality: A Study Of 3d Printing Onboard U.S. Navy Ships, Nick Jensen

Dissertations, Master's Theses and Master's Reports

The current landscape of manufacturing is evolving because of technology like additive manufacturing (AM). The mobility and compactness of AM are what make it desirable for many industry sectors. The U.S. Navy has shown interest in deploying AM on ships as it could alleviate their dependency on off-route docking to recharge supplies. However, the U.S. Navy's aspiration is currently hindered due to harsh ship-borne environments that degrade AM part quality.

This thesis focuses on vertical base vibration effects on AM part quality. An introduction is first given in Chapter 1 to familiarize the reader with the U.S. Navy's predicament. The …

Mathematical Modeling And Numerical Simulation Of Heat Transfer From Isolated Objects, Esmaeil Dehdashti

Dissertations, Master's Theses and Master's Reports

In the area of heat transfer, like other fields of science and engineering, full- and semi-analytical solutions of elementary problems are regarded as invaluable resources that can be used to identify relevant dimensionless parameters, to obtain basic insights into the phenomena under consideration, to quickly quantify the effects of key factors, and, ultimately, to pave the way for understanding more complex problems arising in practice. These solutions can also serve as excellent benchmarks for calibrating experimental setups and validating numerical techniques. In this dissertation, we theoretically study three classical heat transfer problems, with the ultimate goal of deriving analytical or …

Natural Resource Curse, Economic Freedom, Economic Growth And Development: Case Of Africa, Oluwatomisin Shalom Akinbo

Dissertations, Master's Theses and Master's Reports

Over the years, there has been discord or lack of agreement on the effect that natural resources have on economic growth and development. This study adopts output approach to investigate the effect that natural resources and institutions (Economic Freedom) have on economic growth for the period of 1960 to 2020 using World Bank data. The analysis employs Multiple Regression Analysis based on augmented Ordinary Least Squares Regression methods. The results are mixed, as the outcome indicates that, while natural resources are positively related to economic growth, there exists a crowding out effect between natural resources and two sectors of the …

Novel New Modeling Procedure For Industrial Machinery With Nonlinear Connections, Steven Whitican

Dissertations, Master's Theses and Master's Reports

Given current timelines for rolling out production at volume, the ability to model portions of the production process is of paramount importance. To model production, high-fidelity models of production assets must be obtained. The production assets are comprised of linear and nonlinear structures. The linear structures are well understood and defined. Significant work has, and is, being done to understand the nonlinear components. This work focuses on developing and correlating a model of a nonlinear component. The component studied and modeled is a linear guide. Linear guides are bearings which facilitate translational motion of machine axes. An accurate model of …

Multi-Dimensional Modeling For Environmental Impact Assessment At Intersections Of The Food-Energy-Water Nexus, Jessica Daignault

Dissertations, Master's Theses and Master's Reports

This dissertation uses multi-dimensional modeling for environmental impact assessment at intersections of the Food-Energy-Water (FEW) Nexus, including life cycle assessment (LCA) modeling for quantification of environmental impacts due to household FEW consumption, a linear regression framework for quantification of water-use impacts of marginal electricity generation, and a multi-objective optimization model to assess monetization of water withdrawals for electricity generation and impacts to water stress due to electricity dispatch schemes. Chapter 2 of this dissertation summarizes the development of an LCA model that quantifies the direct and indirect environmental impacts of household FEW consumption. The model is executed through a novel …

Local Watershed Analysis For Hubbell And Lake Linden, Michigan, Brittany Hubbard

Dissertations, Master's Theses and Master's Reports

This report analyzes flood frequency risk, examines drainage, and delineates local level watershed characteristics for the Site located in western HUC 12-040201 030304 Torch Lake Subwatershed. Specifically, unnamed catchments in need of HUC-14 or HUC-16 local level delineation are evaluated to determine the effects of excess runoff on the cities of Hubbell and Lake Linden, Michigan. Further, this report aims to identify the location of known or suspected historical and current drainage infrastructure, and to prioritize locations for low-impact sustainable designs that reduce excess runoff and provide flood protection for these lakeshore communities.

Development Of A Propagation Program For Beech Bark Disease-Resistant American Beech (Fagus Grandifolia) And An Applied Restoration Plan For Mitigation Of Beech Bark Disease, Ande Myers

Dissertations, Master's Theses and Master's Reports

This dissertation describes the work accomplished towards mitigation of beech bark disease (BBD) through a joint venture by Michigan Technological University and the National Park Service. American beech is an ecologically important species that is threatened throughout its range by beech bark disease and other newer, emergent pressures such as climate change and beech leaf disease. A literature review is included to synthesize recent advances in American beech (Fagus grandifolia Ehrh.) propagation and their application in mitigation of BBD (Chapter 2). These concepts are examined in an applied restoration framework to outline the importance of understanding ecological and technological …

Sensing Methods For Two-Target And Four-Target Detection In Time-Constrained Vector Poisson And Gaussian Channels, Muhammad Fahad

Dissertations, Master's Theses and Master's Reports

In this dissertation we consider a sensor scheduling or resource management problem for a vector Poisson and Gaussian channels. The input is a binary random vector and the output is a set of conditionally independent Poisson or Gaussian random variables. The objective is to design a scaling matrix, which is a linear transformation whose purpose is to entangle the different inputs, under a total given energy/time constraint. The two metrics are adopted to quantify the performance of the designed scaling matrix: mutual information and Bayesian inference. In other words, it is an experimental design problem where the objective is to …

Active Gravity Offloading System With Infrared Tracking For Rover Testing, Travis Wavrunek

Dissertations, Master's Theses and Master's Reports

Gravity offloading is a tool used to test how different gravitational forces will impact the mobility of rovers bound for Lunar or Martian expeditions. Previous approaches have been successful in simulating partial gravity environments, and this report details how the Infrared- Gravity offload (IRGO) system, developed for the Planetary Surface Technology Development Laboratory (PSTDL) and lunar simulant sandbox, has a similar aim. Through a series of iterations, IRGO has been developed to actively track an infrared beacon and follow a rover within the test chamber to eliminate inertial and friction forces along two horizonal axes. A portion of a rover’s …

Comparing Scoop3d And Gis-Tissa Models For Slope Stability Analysis In Idukki, Kerala, India, Stepan Pikul

Dissertations, Master's Theses and Master's Reports

Landslides are the most destructive hazard in the mountainous Idukki district in the State of Kerala, India. Therefore, evaluating the possible occurrence of landslides and analyzing the factors that trigger failure is an essential part of a reliable landslide assessment. Physics-based models are commonly used to determine potential landslide susceptible areas in terms of Factor of Safety (FS). Recent years have seen the use of physics-based methods for regional-scale landslide susceptibility analysis using geospatial tools. In this study, we compare two physics-based models using the same data from Idukki. The two models are the Geographic Information System-Tool for Infinite Slope …

Marangoni Propulsion Of Active Particles, Saeed Jafari Kang

Dissertations, Master's Theses and Master's Reports

We study the surfing motion of active particles located at a flat liquid-gas interface. The particles create and maintain a surface tension gradient by asymmetrically discharging a surface tension-reducing agent. We employ theory and numerical simulation to investigate the Marangoni propulsion of these active surfers. First, we use the reciprocal theorem to establish a relationship between the propulsion speed and the release of the active chemical. This theoretical relation is utilized to examine the effect of wall confinement and geometry on the Marangoni-driven motion of active particle when the inertial effects are negligible and when the transports of the released …

Hydrodynamics Of Vibrating Perforated Plates, Muhammad Usman

Dissertations, Master's Theses and Master's Reports

The objective of this Ph.D. project is to study the hydrodynamic response of oscillating perforated plates by utilizing analytical and numerical methods. Specifically, we will examine whether the permeable plates offer superior hydrodynamic stability than their impermeable equivalents. First, we will computationally investigate the linear hydrodynamic response of vibrating perforated plates in an unbounded domain in the asymptotic limit of small KC, i.e., KC → 0. We will simulate various porosities and aspect ratios for a wide range of oscillatory Reynolds number (denoted by β) and seek to draw a comparison between our findings and the analytical works. Next, we …

A Resolution Of Grain Boundary Strengthening Mechanisms By Nanoindentation Induced Local Mechanical Response, Prasad Pramod Soman

Dissertations, Master's Theses and Master's Reports

Grain boundary segregation is well known to cause significant embrittlement of alloys. But in certain cases, it has also been observed to increase mechanical strength. This project attempts to assess local mechanical behavior of specific grain boundaries with and without segregation in order to understand association between grain boundary chemistry and deformation mechanism utilizing instrumented nanoindentation technique. It is hypothesized that solute segregation strongly affects the grain boundary energy which in turn affects the deformation mechanism processes. This project also utilizes a unique ability provided by the instrumented indentation technique to interrogate local grain boundary strengthening mechanisms proposed by Hall-Petch …

Evening And Overnight Cardiovascular And Arterial Stiffness Responses To Simulated Binge Drinking, Grant Thivierge

Dissertations, Master's Theses and Master's Reports

Acute alcohol consumption has been shown to increase blood pressure, while chronic heavy alcohol consumption is associated with the development of hypertension. Another factor that contributes to hypertension is arterial stiffness. While evidence suggests light to moderate alcohol consumption can decrease arterial stiffness, the impact of binge alcohol consumption on acute and overnight changes of arterial stiffness remains unknown. Twenty-seven participants (14 female; 13 male) received a binge alcohol dose or fluid control randomly one month apart. Alcohol or fluid control doses were administered in two equally divided allocates at 8 and 9 pm. A 1:3 mixture of 190 proof …

Experimental Evaluation And Simulation Of Torque Transmissibility Frequency Response Functions Of Vibration Isolators And Absorbers For Drivetrain Applications, Luke Jurmu

Dissertations, Master's Theses and Master's Reports

Four studies involving torsional vibration isolation performance of automotive drivetrain components, make up this dissertation. One study features a prototype planetary torsional vibration absorber, a unique device that targets low frequency torsion modes in automotive drivetrains. Two studies feature experiments on several torque converters, clutch locked and open, to validate models of the hardware. The last study details experiments on a centrifugal pendulum absorber in a torque converter, to characterize the viscous friction while submerged in automatic transmission fluid (ATF). The enclosed studies improve the state of the art of drivetrain vibration absorbers and isolators, by introducing a new vibration …

Understanding The Weather- And Soil-Related Variability In Agricultural Water Footprints: Case Study Of Maize And Soybeans Grown In The St. Joseph Watershed, Karleigh M. Krieg

Dissertations, Master's Theses and Master's Reports

Introduced in 2002, the water footprint is a valuable tool for understanding the consumption of freshwater resources. The traditional approach to quantifying the water footprint is to sum the green, blue, and gray water footprint components. The green water footprint is the volume of water that comes from precipitation, is stored in the soil, and used by vegetation. The blue water footprint is the volume of surface or ground water that is withdrawn and applied to cultivated lands via irrigation. These components are based on the evapotranspiration of green and blue water resources, respectively. The gray water footprint is the …

An Overview Of Converting Kabul’S Traffic Circles And Rotaries To Modern Roundabouts, Mohammad Anas Taeb

Dissertations, Master's Theses and Master's Reports

Circular intersections, in the form of traffic circles and rotaries, were built around the world in the first half of the twentieth century. However, with the rise in traffic flow, regular traffic congestion and accidents forced authorities to look for alternative solutions and, in many cases, signalized intersections became the preferred alternative. In the 1960s, the UK introduced concept of modern roundabouts and a new set of priority rules for the circulating traffic. Since the inception of modern roundabouts, they have enhanced both operational and safety aspects at these locations, even when used to replace large traffic circles/rotaries, proving that …

The Influence Of Decreased Ambient Lighting On Reactive Balance Mechanisms In Young Adults, Jessica Pitts

Dissertations, Master's Theses and Master's Reports

Introduction: Balance recovery/reactive balance prevents falls by restabilizing the center of mass (COM) when instability occurs. Dim lighting is a risk factor for falling, although limited research has examined how lighting conditions affect the ability to recover from losses of balance. The purpose of this thesis was to examine the effect of lighting conditions on reactive balance mechanisms. Methods: 20 young adults (23.3(4.4) y) completed forward lean-and-release perturbations in two lighting conditions: 1) Light: ~800 Lux; 2) Dark: 0 Lux. Optical motion capture and surface electromyography were used to quantify stepping/angular kinematics, COM control, and the timing, activation, and coordination …

Age, Growth, And Otolith Microchemistry Of Golden Tilefish (Lopholatilus Chamaeleonticeps) In The Nw Atlantic, Katy Dawson

Dissertations, Master's Theses and Master's Reports

Knowledge of life-history characteristics and patterns of connectivity are important parameters to fisheries management, especially for species inhabiting hard to reach environments, such as the deep-sea. Golden Tilefish (Lopholatilus chamaeleonticeps) are slow growing, long-lived, demersal species that exhibit a patchy distribution along the continental shelf-edge of the NW Atlantic Ocean. Golden Tilefish create burrows in the clay sediment and maintain high site fidelity. These characteristics suggest the possibility of localized subpopulations across the species’ distribution; an important consideration for the resilience of this species to fishing pressure. My objectives are (1) to estimate age, and model growth of …

Anterior Cruciate Ligament Injury Incidence Across Sex, Sport, And Level: A Systematic Review And Meta-Analysis, Dana Anderson

Dissertations, Master's Theses and Master's Reports

Well controlled Anterior Cruciate Ligament (ACL) injury prevention neuromuscular training reduces the risk of ACL injury by 50%, but despite the efficacy of these programs, ACL injury rates have not decreased. This lack of decrease in injury incidence may be due in part to limited knowledge on who is most at risk for sustaining an ACL injury, and there is no formal clinical tool available to estimate the personalized or group risk of ACL injury. The purpose of this study was to better understand what groups of athletes are most at risk of sustaining a primary ACL injury, as the …

Unmanned Ground Vehicle (Ugv) Docking, Connection, And Cabling For Electrical Power Transmission In Autonomous Mobile Microgrids, John Naglak

Dissertations, Master's Theses and Master's Reports

Autonomous Mobile Microgrids provide electrical power to loads in environments where humans either can not, or would prefer not to, perform the task of positioning and connecting the power grid equipment. The contributions of this work compose an architecture for electrical power transmission by Unmanned Ground Vehicles (UGV). Purpose-specific UGV docking and cable deployment software algorithms, and hardware for electrical connection and cable management, has been deployed on Clearpath Husky robots. Software development leverages Robot Operating System (ROS) tools for navigation and rendezvous of the autonomous UGV robots, with task-specific visual feedback controllers for docking validated in Monte-Carlo outdoor trials …

Commissioning And Testing Of A New Dusty Thermal Vacuum Chamber, Ben Wiegand

Dissertations, Master's Theses and Master's Reports

The Dusty Thermal Vacuum Chamber (DTVAC) is a new facility purchased by the Planetary Surface Technology Development Lab (PSTDL) that will be used to test the Technology Readiness Level (TRL) of extraplanetary devices and systems. With the use of vacuum pumps and simulated regolith, the DTVAC can create extraplanetary environments such as those found on the Moon and Mars. This report details the actions that were taken to prepare the DTVAC for TRL testing, including the development of a Data Acquisition Center (DAC) and test fixtures, along with the findings of baseline tests that were performed to understand the behavior …

Towards Systems On Cloth: The Design, Manufacturing, And Validation Of Open-Source Embroidered Resistors, Somerset Schrock

Dissertations, Master's Theses and Master's Reports

This thesis is focused on advancing embroidered wearable electronics and textile electronics by creating open-source flexible resistors. Advancements in textile electronics could usher a new generation of smart devices that are entirely flexible. Current systems on cloth primarily use rigid components, which limits the flexibility and comfort of using the fabric devices. To advance this field, I propose a novel method of creating flexible electrical resistors with embroidery. To realize this technology, I created an open-source tool to create embroidery files for machine fabrication. This thesis details the methods and tools created for resistor fabrication. The resistors were tested to …

Comparative Study Of Biocides And Nanoparticles On Bacterial Microorganisms, Rehab Alhajjar

Dissertations, Master's Theses and Master's Reports

The increase in antimicrobial-resistant bacteria has posed challenges to treating resistant diseases. Antimicrobials such as nanoparticles (NPs) and biocides are commonly used to control microbial growth in household and industrial settings. Several studies have shown that bacteria that become resistant to biocides can also resist antibiotics. There are fewer reports of resistance to nanoparticles. Our study goals were to compare the environmental effects of biocides and nanoparticles by investigating their impacts on stream microbial community structure, the mechanism of bacterial resistance, and their interactions with natural organic matter (NOM). Our results showed that biocides dramatically altered microbial community composition and …