Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 5 of 5
Full-Text Articles in Entire DC Network
Studies On The Human And Bovine Spinal Cord Protein, Kevin Grant Weir
Studies On The Human And Bovine Spinal Cord Protein, Kevin Grant Weir
Digitized Theses
This report contributes to the knowledge of antiencephalitogenic spinal cord protein (SCP) with respect to three major areas of study: (1) the purification and characterization of human SCP; (2) the nature of the association of SCP with nervous tissue components and (3) purification of cyanogen bromide-derived peptides of bovine SCP.;Human SCP and a protein immunochemically identical to SCP (SCP-PN) were purified from spinal cords and peripheral nerves with 0.15 M sodium chloride, carboxy-methyl cellulose chromatography and gel filtration on Sephadex G-50 superfine. SCP and SCP-PN had estimated molecular weights of 13,700 and 14,700 daltons, respectively and had similar amino acid …
Synthetic And Structural Studies On Chiral Fluorinated Imino- And Amino-Alcohols And Their Copper(Ii) Complexes, Stephen Joseph Loeb
Synthetic And Structural Studies On Chiral Fluorinated Imino- And Amino-Alcohols And Their Copper(Ii) Complexes, Stephen Joseph Loeb
Digitized Theses
This thesis describes a preparative route to chiral fluorinated amino-alcohol ligands via the Schiff base template condensation reaction. The synthetic route consists of three main steps; (1) template condensation of a primary amine with 5,5,5-trifluoro-5-hydroxy-4-(trifluoromethyl)-2-pentanone, HFDA, in the presence of Cu('2+) ions, (2) hydride reduction of the resulting Schiff base copper(II) complex, and (3) isolation of the optically active ligand. Two new types of ligands are prepared; bidentate imino- and amino-alcohol ligands derived from monofunctional amines, R-NH(,2), and tridentate imino- and amino-diol ligands derived from bifunctional aminoalcohols, NH(,2)-OH.;The bidentate ligands are uninegative and form CuL(,2) complexes with a square planar …
Regulation Of Cyclic-Amp Phosphodiesterase Of Rat Skeletal Myoblasts, Prem Kumar Seth
Regulation Of Cyclic-Amp Phosphodiesterase Of Rat Skeletal Myoblasts, Prem Kumar Seth
Digitized Theses
The aim of the present investigation was to study the various regulatory mechanisms involved in the control of cAMP phosphodiesterases in rat skeletal myoblasts.;In rat skeletal myoblasts and adult muscle extracts, four forms of a high affinity cAMP phosphodiesterase are found in vitro. These are termed PDE I, PDE II, PDE III and PDE IV, and have approximately molecular weights of 1.5 x 10('6), 400,000, 120,000 and 60,000, respectively. Evidence is presented to show that there is only one primary form of phosphodiesterase in myoblasts, viz. PDE II, with the rest of the forms being derived from it.;In the presence …
Guinea Pig Hepatic Microsomal 16 Alpha-Hydroxylation Of Estrone-3-Sulfate, Christopher James Tsoulis
Guinea Pig Hepatic Microsomal 16 Alpha-Hydroxylation Of Estrone-3-Sulfate, Christopher James Tsoulis
Digitized Theses
Hydroxylation reactions play an important role in steroid metabolism. The enzyme systems responsible for most steroid hydroxylations are believed to be mixed function oxidases usually acting with reduced pyridine nucleotides as electron donors and cytochrome P450 as the terminal oxidase. A reliable procedure for the assay of live microsomal 16(alpha)-hydroxylation of estrone-3-sulfate has been developed for the guinea pig. It is based on the rapid, quantitative separation of estradiol and estriol by Sephadex LH-20 columns after the chemical reduction and hydrolysis of the incubation products.;Microsomal preparations and incubation conditions that optimized the 16(alpha)-hydroxylation of estrone-3-sulfate were employed. Under these circumstances, …
Thyroid Hormone Action In Cultured Hepatocytes, Elizabeth Jane Wilson
Thyroid Hormone Action In Cultured Hepatocytes, Elizabeth Jane Wilson
Digitized Theses
The object of the research in this thesis was to demonstrate and characterize the actions of thyroid hormones at the cellular level. It was necessary to develop a cell culture system responsive to thyroid hormones. A method for the primary culturing of adult rat hepatocytes in serum-free medium is described. Culture surfaces were coated with a film of rat tail collagen which increased the longevity of the cells in culture. The addition of insulin or dexamethasone to the culture medium altered the morphology of the cells when compared to hepatocytes maintained in hormone-free medium. Insulin and dexamethasone, together, substantially improved …