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Structural Basis For Human Anti-Dna Antibody Specificity, Mahmoud Mahmoudi
Structural Basis For Human Anti-Dna Antibody Specificity, Mahmoud Mahmoudi
Digitized Theses
Anti-DNA antibodies are hallmarks of SLE both in human and inbred lupus prone mice. The role of these antibodies in disease pathogenesis, particularly in glomerulonephritis has been documented. It is now well accepted that the genetic potential to generate anti-DNA antibodies also exists in the normal B cell immune repertoire. This genetic potential has been revealed by detection of anti-DNA antibodies in serum of normal individuals and by immortalizing DNA reactive B cells as hybridomas and as transformed cell lines. There is growing evidence that anti-DNA antibodies from the normal B cell repertoire and disease-associated anti-DNA antibodies share V region …
Image Correlation Spectroscopy: Development And Application To Studies Of Pdgf Receptor Distributions, Paul William Wiseman
Image Correlation Spectroscopy: Development And Application To Studies Of Pdgf Receptor Distributions, Paul William Wiseman
Digitized Theses
Aggregation of macromolecular plasma membrane receptors following ligand binding is hypothesized to be the initial step in signal transduction in cellular systems. Quantitative measurement of receptor distributions on cell surfaces is integral for elucidating the complicated mechanisms involved in transduction of chemical signals across the cells membrane. Scanning fluorescence correlation spectroscopy (S-FCS) has been used successfully to perform quantitative measurements of receptor densities and aggregate sizes. However, these measurements take a great deal of time, and require specialized equipment to implement. Here, image correlation spectroscopy (ICS) is introduced as a novel extension of scanning fluorescence correlation spectroscopy that improves on …
Developmental Potential Of Rat Myoblast Lineages, Christopher Louis Pin
Developmental Potential Of Rat Myoblast Lineages, Christopher Louis Pin
Digitized Theses
Skeletal muscle consists of multinucleated fibres which are classified as slow (I) or fast (IIA, IIB, IIX) muscle fibre types which differ in their contractile properties and their expression of contractile protein isoforms. Individual muscles contain characteristic distributions of fibre types which can be identified based on their myosin heavy chain (MyHC) content. While classical studies have demonstrated that the fibre type composition of adult muscles can be altered by extrinsic factors (such as changes in the pattern of innervation), recent studies suggest that different fibre types may be formed from distinct myoblast lineages. The purpose of this study was …
High Resolution And Wideline Nmr Studies Of Membrane Associated Molecules Involved In Signal Transduction, Alan Cavell Rigy
High Resolution And Wideline Nmr Studies Of Membrane Associated Molecules Involved In Signal Transduction, Alan Cavell Rigy
Digitized Theses
The primary focus of our investigations were centered around understanding the biochemical basis of the early events involved in signal transduction. Our work has involved two novel systems: (1) sphingolipid metabolites and (2) a synthetic peptide representing the transmembrane region of the human epidermal growth factor receptor (hEGFR).;Sphingolipid metabolites have emerged as physical modulators of many signalling pathways, including cell growth regulation, and signal transduction. N,N-({dollar}\sp2{dollar}H{dollar}\sb6{dollar}) -dimethylsphingosine and ({dollar}\sp2{dollar}H{dollar}\sb4{dollar}) -lysogalactosyl ceramide were produced by partial synthesis and studied via wideline {dollar}\sp2{dollar}H NMR, as a function of pH. Spectra of ({dollar}\sp2{dollar}H{dollar}\sb6{dollar}) -dimethylsphingosine identified that the molecule's orientation and behaviour are largely …
The Influence Of Extremely Low Frequency Magnetic Fields On Cytosolic-Free Calcium In Human Cells, Jeffrey John Carson
The Influence Of Extremely Low Frequency Magnetic Fields On Cytosolic-Free Calcium In Human Cells, Jeffrey John Carson
Digitized Theses
Extremely low frequency (ELF) magnetic fields have been reported to affect Ca{dollar}\sp{lcub}2+{rcub}{dollar}dependent biological processes. Based on these reports, I hypothesized that cystosolic free calcium concentration ({dollar}\lbrack\rm Ca\sp{lcub}2+{rcub}\rbrack\sb{lcub}i{rcub}){dollar} may be influenced by ELF magnetic fields and found an increase in HL-60 cells following exposure {dollar}(P0.09).{dollar} However, experiments with indo-1-loaded Jurkat cells indicated frequency- and field strength-dependent effects on resting and mitogen-stimulated {dollar}\lbrack\rm Ca\sp{lcub}2+{rcub}\rbrack\sb{lcub}i{rcub}{dollar} levels {dollar}(P<0.01).{dollar} The findings show that ELF magnetic fields affect {dollar}\lbrack\rm Ca\sp{lcub}2+{rcub}\rbrack\sb{lcub}i{rcub}{dollar} and may explain some of the reported Ca{dollar}\sp{lcub}2+{rcub}{dollar}-dependent biological effects of ELF magnetic fields.