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Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt
Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt
Department of Civil and Environmental Engineering: Faculty Publications
The use of wastewater for irrigation may introduce antimicrobials and human pathogens into the food supply through vegetative uptake. The objective of this study was to investigate the uptake of three antimicrobials and Salmonella in two lettuce cultivars. After repeated subirrigation with synthetic wastewater, lettuce leaves and soil were collected at three sequential harvests. The internalization frequency of Salmonella in lettuce was low. A soil horizon-influenced Salmonella concentration gradient was determined with concentrations in bottom soil 2 log CFU/g higher than in top soil. Lincomycin and sulfamethoxazole were recovered from lettuce leaves at concentrations as high as 822 ng/g and …
Influence Of Grass Hedges On The Transport Of Antimicrobials, Antimicrobial Resistance Genes, And Antimicrobial Resistant Pathogens After Land Application Of Swine Manure, Shannon L. Bartelt-Hunt
Influence Of Grass Hedges On The Transport Of Antimicrobials, Antimicrobial Resistance Genes, And Antimicrobial Resistant Pathogens After Land Application Of Swine Manure, Shannon L. Bartelt-Hunt
Department of Civil and Environmental Engineering: Faculty Publications
The objective of this study was to determine the effects of narrow grass hedges on the fate and transport of antimicrobials and antimicrobial resistance genes (ARGs) in runoff and in soil following the land application of swine manure slurry. Swine manure slurry was land applied to 0.75m wide by 4.0m long plots established on an Aksarben silty clay loam soil located in southeast Nebraska. Swine manure was applied at a rate to meet the 3-year nitrogen (N) requirements for corn. Swine manure was applied to plots with and without a narrow grass hedge to evaluate the effect of the hedge …
Effects Of The Antimicrobial Tylosin On The Microbial Community Structure Of An Anaerobic Sequencing Batch Reactor, Toshio Shimada, Xu Li, Julie L. Zilles, Eberhard Morgenroth, Lutgarde Raskin
Effects Of The Antimicrobial Tylosin On The Microbial Community Structure Of An Anaerobic Sequencing Batch Reactor, Toshio Shimada, Xu Li, Julie L. Zilles, Eberhard Morgenroth, Lutgarde Raskin
Department of Civil and Environmental Engineering: Faculty Publications
The effects of the antimicrobial tylosin on a methanogenic microbial community were studied in a glucosefed laboratory-scale anaerobic sequencing batch reactor (ASBR) exposed to stepwise increases of tylosin (0, 1.67, and 167 mg/L). The microbial community structure was determined using quantitative fluorescence in situ hybridization (FISH) and phylogenetic analyses of bacterial 16S ribosomal RNA (rRNA) gene clone libraries of biomass samples. During the periods without tylosin addition and with an influent tylosin concentration of 1.67 mg/L, 16S rRNA gene sequences related to Syntrophobacter were detected and the relative abundance of Methanosaeta species was high. During the highest tylosin dose of …