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Articles 1 - 6 of 6
Full-Text Articles in Entire DC Network
Antimicrobial Resistance Determinants Circulating Among Thermophilic Campylobacter Isolates Recovered From Broilers In Ireland Over A One-Year Period, Caoimhe T. Lynch, Helen Lynch, Sarah Burke, Kayleigh Hawkins, Colin Buttimer, Conor Mc Carthy, John Egan, Paul Whyte, Declan Bolton, Aidan Coffey, Brigid Lucey
Antimicrobial Resistance Determinants Circulating Among Thermophilic Campylobacter Isolates Recovered From Broilers In Ireland Over A One-Year Period, Caoimhe T. Lynch, Helen Lynch, Sarah Burke, Kayleigh Hawkins, Colin Buttimer, Conor Mc Carthy, John Egan, Paul Whyte, Declan Bolton, Aidan Coffey, Brigid Lucey
Department of Biological Sciences Publications
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Campylobacteriosis is the leading cause of human bacterial gastroenteritis, very often associated with poultry consumption. Thermophilic Campylobacter (Campylobacter jejuni and Campylobacter coli) isolates (n = 158) recovered from broiler neck skin and caecal contents in Ireland over a one-year period, resistant to at least one of three clinically relevant antimicrobial classes, were screened for resistance determinants. All ciprofloxacin-resistant isolates (n = 99) harboured the C257T nucleotide mutation (conferring the Thr-86-Ile substitution) in conjunction with other synonymous and nonsynonymous mutations, which may have epidemiological value. The A2075G nucleotide mutation and amino acid …
The Impact Of Key Processing Stages And Flock Variables On The Prevalence And Levels Of Campylobacter On Broiler Carcasses, Malgorzata Emanowicz, Joseph Meade, Declan Bolton, Olwen Golden, Montserrat Gutierrez, William Byrne, John Egan, Helen Lynch, Lisa O'Connor, Aidan Coffey, Brigid Lucey, Paul Whyte
The Impact Of Key Processing Stages And Flock Variables On The Prevalence And Levels Of Campylobacter On Broiler Carcasses, Malgorzata Emanowicz, Joseph Meade, Declan Bolton, Olwen Golden, Montserrat Gutierrez, William Byrne, John Egan, Helen Lynch, Lisa O'Connor, Aidan Coffey, Brigid Lucey, Paul Whyte
Department of Biological Sciences Publications
This study examined the impact of key processing stages and flock variables on the prevalence of Campylobacter on broiler carcasses. Overall, the prevalence of Campylobacter was 62% in caeca, and 68%, 65% and 62% in neck skin samples collected after evisceration, final wash and carcass chilling, respectively. Campylobacter were found in 32% of caeca, and 52%, 40% and 32% of neck skin samples collected after evisceration, final wash and carcass chilling, respectively from first thin broiler batches. Final thin broiler batches were more frequently contaminated with prevalences of 83% found in caeca, 80% in neck skin samples collected after evisceration …
Investigation Of Molecular Mechanisms Underlying Tetracycline Resistance In Thermophilic Campylobacter Spp. Suggests That Previous Reports Of Tet(A)-Mediated Resistance In These Bacteria Are Premature, Caoimhe Lynch, Kayleigh Hawkins, Helen Lynch, John Egan, Declan Bolton, Aidan Coffey, Brigid Lucey
Investigation Of Molecular Mechanisms Underlying Tetracycline Resistance In Thermophilic Campylobacter Spp. Suggests That Previous Reports Of Tet(A)-Mediated Resistance In These Bacteria Are Premature, Caoimhe Lynch, Kayleigh Hawkins, Helen Lynch, John Egan, Declan Bolton, Aidan Coffey, Brigid Lucey
Department of Biological Sciences Publications
The true prevalence of tet(A), which codes for a tetracycline efflux pump, in thermophilic Camplyobacter spp. requires clarification after reports emerged in Iran (2014) and Kenya (2016) of the novel detection of tet(A) in Campylobacter. During our investigation of antibiotic resistance mechanisms in a sample of Irish thermophilic Campylobacter broiler isolates, it was determined that 100% of tetracycline-resistant isolates (n = 119) harboured tet(O). Accessory tetracycline-resistance mechanisms were considered as tetracycline minimum inhibitory concentrations ranged from 4 to ≥ 64 mg/L. Primers previously reported for the detection of tet(A) in Campylobacter failed to produce an amplicon using a positive control …
Genomic Investigation Into Strain Heterogeneity And Pathogenic Potential Of The Emerging Gastrointestinal Pathogen Campylobacter Ureolyticus, Susan Bullman, Alan Lucid, Roy D. Sleator, Brigid Lucey, Et. Al.
Genomic Investigation Into Strain Heterogeneity And Pathogenic Potential Of The Emerging Gastrointestinal Pathogen Campylobacter Ureolyticus, Susan Bullman, Alan Lucid, Roy D. Sleator, Brigid Lucey, Et. Al.
Department of Biological Sciences Publications
The recent detection and isolation of C. ureolyticus from patients with diarrhoeal illness and inflammatory bowel diseases warrants further investigation into its role as an emerging pathogen of the human gastrointestinal tract. Regarding the pathogenic mechanisms employed by this species we provide the first whole genome analysis of two C. ureolyticus isolates including the type strain. Comparative analysis, subtractive hybridisation and gene ontology searches against other Campylobacter species identifies the high degree of heterogenicity between C. ureolyticus isolates, in addition to the identification of 106 putative virulence associated factors, 52 of which are predicted to be secreted. Such factors encompass …
Campylobacter Ureolyticus: An Emerging Gastrointestinal Pathogen?, Susan Bullman, Daniel Corcoran, James O'Leary, Brigid Lucey, Deirdre Byrne, Roy D. Sleator
Campylobacter Ureolyticus: An Emerging Gastrointestinal Pathogen?, Susan Bullman, Daniel Corcoran, James O'Leary, Brigid Lucey, Deirdre Byrne, Roy D. Sleator
Department of Biological Sciences Publications
A total of 7194 faecal samples collected over a 1-year period from patients presenting with diarrhoea were screened for Campylobacter spp. using EntericBios, a multiplex-PCR system. Of 349 Campylobacter-positive samples, 23.8% were shown to be Campylobacter ureolyticus, using a combination of 16S rRNA gene analysis and highly specific primers targeting the HSP60 gene of this organism. This is, to the best of our knowledge, the first report of C. ureolyticus in the faeces of patients presenting with gastroenteritis and may suggest a role for this organism as an emerging enteric pathogen.
Integronlike Structures In Campylobacter Spp. Of Human And Animal Origin, Brigid Lucey, D. Crowley, P. Moloney, B. Cryan, M. Daly, F. O'Halloran, E. J. Threlfall, S. Fanning
Integronlike Structures In Campylobacter Spp. Of Human And Animal Origin, Brigid Lucey, D. Crowley, P. Moloney, B. Cryan, M. Daly, F. O'Halloran, E. J. Threlfall, S. Fanning
Department of Biological Sciences Publications
Resistance to antimicrobial agents used to treat severe Campylobacter spp. gastroenteritis is increasing worldwide. We assessed the antimicrobial resistance patterns of Campylobacter spp. isolates of human and animal origin. More than half (n = 32) were resistant to sulphonamide, a feature known to be associated with the presence of integrons. Analysis of these integrons will further our understanding of Campylobacter spp. epidemiology.