Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 30 of 90
Full-Text Articles in Entire DC Network
Astroglial Insulin-Like Growth Factor Binding Proteins: Regulation Of Synthesis And Relationship To Cell Growth, Sheri Lynn Bradshaw
Astroglial Insulin-Like Growth Factor Binding Proteins: Regulation Of Synthesis And Relationship To Cell Growth, Sheri Lynn Bradshaw
Digitized Theses
The insulin-like growth factors (IGFs) regulate the growth and differentiation of a wide variety of tissues, including the brain. The biologic actions of IGFs are modulated by a family of six IGF binding proteins (IGFBPs), which, like IGFs, are synthesized as paracrine factors. We hypothesized that the IGFBPs synthesized by astroglial cells, are regulated by growth factors produced in the developing brain, and modulate the growth of these cells. Primary astroglial cells synthesized IGFBP-2 and IGFBP-3 which were upregulated in a dose dependent manner by IGF-I, IGF-II, and very high concentrations of insulin, suggesting that the growth factors regulate their …
Properties And Regulation Of The Calcium Ion Pump In Heart And Skeletal Muscle Sarcoplasmic Reticulum, Cynthia Elaine Hawkins
Properties And Regulation Of The Calcium Ion Pump In Heart And Skeletal Muscle Sarcoplasmic Reticulum, Cynthia Elaine Hawkins
Digitized Theses
In heart and skeletal muscle, the sarcoplasmic reticulum (SR) plays a critical role in the contraction-relaxation cycle of the myocyte: a Ca{dollar}\sp{lcub}2+{rcub}{dollar} channel releases Ca{dollar}\sp{lcub}2+{rcub}{dollar} from SR into cytoplasm to trigger contraction, and a Ca{dollar}\sp{lcub}2+{rcub}{dollar} pump resequesters Ca{dollar}\sp{lcub}2+{rcub}{dollar} to cause relaxation. A major regulator of this cycle is {dollar}\rm Ca\sp{lcub}2+{rcub}{dollar}/calmodulin-dependent protein kinase (CaM kinase), which is known to phosphorylate and thereby activate the cardiac SR Ca{dollar}\sp{lcub}2+{rcub}{dollar} pump, Ca{dollar}\sp{lcub}2+{rcub}{dollar} channel and endogenous Ca{dollar}\sp{lcub}2+{rcub}{dollar}-pump inhibitor phospholamban. This thesis examines the Ca{dollar}\sp{lcub}2+{rcub}{dollar} pump and its regulation in different tissues (cardiac, slow-twitch and fast-twitch muscle SR: CMSR, SMSR and FMSR) and different stages in …
Some Physiological Effects Of Stress In The American Cockroach Periplaneta Americana (L), Shigui Liu
Some Physiological Effects Of Stress In The American Cockroach Periplaneta Americana (L), Shigui Liu
Digitized Theses
The effects of different forms of stress on the American cockroach, Periplaneta americana (L.) have been systematically examined. This study demonstrated that at least two stress-related events occurred in the haemolymph of the American cockroach. First, the data clearly indicated that a large increase in hypertrehalosemic hormones occurred in the haemolymph of stressed cockroaches. Secondly, proteolytic activity in the haemolymph of stressed cockroaches was greatly increased. The increase of both hypertrehalosemic hormones and proteolytic activity in the haemolymph appeared to be a non-specific response to different forms of stress, including chemical poisoning with lindane, immobilization, forced movement, starvation, and elevated …
Differential Effects Of Ovarian Hormones On The Left And Right Cerebral Hemispheres: Evidence From Perceptual And Motor Asymmetries, Larissa Araxe Mead
Differential Effects Of Ovarian Hormones On The Left And Right Cerebral Hemispheres: Evidence From Perceptual And Motor Asymmetries, Larissa Araxe Mead
Digitized Theses
In the present series of experiments, the possibility that ovarian hormones act differentially on the left and right hemispheres of the adult human brain was investigated through the assessment of several behavioral asymmetries. Lateralized behaviors or performance patterns are believed to arise through underlying functional or neurochemical asymmetries in the brain. Therefore, an asymmetric effect of ovarian hormones on the underlying brain areas may be revealed as an alteration in the laterality of these behavioral patterns.;The first study involved the administration of a battery of visual and auditory perceptual asymmetry tests to a group of young women. Test sessions took …
Testing For Mate Choice And Competition In Convict Cichlids Using Ecologically Relevant Contexts, Diane Kathleen Urban
Testing For Mate Choice And Competition In Convict Cichlids Using Ecologically Relevant Contexts, Diane Kathleen Urban
Digitized Theses
Mate choice and intrasexual competition in convict cichlids, Cichlasoma nigrofasciatum, was investigated in the laboratory, during courtship, nest building, guarding eggs, and guarding fry. During courtship, females were expected to use male body size to indicate quality and to compete for large males. Females and newly introduced males were tested under two conditions: Treatment1--the males were similar in body length; and Treatment 2--one male was larger than the other two. No significant difference was found between treatments in the frequency of female proximity to males or in the frequency of female-female aggressive behaviour. However, the large male of Treatment 2 …
Neurophysiology Of Spared Motor Tracts In Spinal Cord Injury, Dalton Louis Wolfe
Neurophysiology Of Spared Motor Tracts In Spinal Cord Injury, Dalton Louis Wolfe
Digitized Theses
Recent experimental and therapeutic initiatives have been directed towards enhancing the survival and function of preserved central axons following spinal cord injury (SCI). The continued development of these initiatives depends largely on the sensitivity of techniques to detect the presence of residual innervation in descending motor tracts. Detection of preserved innervation in SCI patients provided the focus of the present thesis.;Preserved motor innervation was investigated in patients with established SCI using transcranial magnetic stimulation of the motor cortex to elicit motor evoked potentials (MEPs) in muscles innervated below the level of the lesion. In particular, a set of experiments was …
The Antioxidant Enzymes (Superoxide Dismutase, Catalase And Glutathione Peroxidase): Their Molecular Evolution And Regulation In Mice, Nicholas Joseph Schisler
The Antioxidant Enzymes (Superoxide Dismutase, Catalase And Glutathione Peroxidase): Their Molecular Evolution And Regulation In Mice, Nicholas Joseph Schisler
Digitized Theses
Reactive, oxygen derived free radicals are detoxified by an enzymatic pathway RA-5 which includes the enzymes superoxide dismutase (CuZn and Mn SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). In an effort to understand the molecular evolution and antiquity of these enzymes, comparative techniques were developed for identification and visualization of protein family relationships. Analysis of amino acid sequence data indicated that these enzymes had expected phylogenetic relationships and that the two SOD enzymes were evolutionarily convergent. Multiple sequence alignments of the SOD, CAT and GSH-Px families of proteins were represented quantitatively and used to probe the Swiss-PROT database for proteins …
Male Reproductive Success And The Mating System Of Bushy-Tailed Woodrats, Michael Graham Topping
Male Reproductive Success And The Mating System Of Bushy-Tailed Woodrats, Michael Graham Topping
Digitized Theses
Bushy-tailed woodrats are nocturnal cricetid rodents distributed throughout the mountainous regions of western North America. Despite a number of studies on the population ecology of this species, little is known about the behaviour of males during the breeding season.;I investigated the reproductive behaviour of male bushy-tailed woodrats in three ways. First, I examined the spatial distribution of individuals within a breeding population, using radiotelemetry and mark-recapture livetrapping. Both males and females exhibited considerable overlap with animals of both sexes, mean intersexual and intrasexual overlap values for each sex did not differ in two of the three years of study. This …
Seed Colour Variation, Dormancy, And Seed Banks In The Bull Thistle, Cirsium Vulgare (Savi) Ten, Colleen Doucet
Seed Colour Variation, Dormancy, And Seed Banks In The Bull Thistle, Cirsium Vulgare (Savi) Ten, Colleen Doucet
Digitized Theses
The bull thistle, Cirsium vulgare (Savi) Ten. is listed as a noxious weed in Ontario and in Australia. It is widespread and found in many disturbed habitats such as roadsides, quarries, construction sites and pastures. Successful weeds are considered to demonstrate, among others, the following features: the production of a large number of small seeds, long-lived seeds, the presence of an efficient dispersal mechanism, and self-compatibility. Although seed production is substantial and Cirsium vulgare is self-compatible, the species has several characteristics that should constrain its demographic success. Seed dispersal is limited and in past studies, this species had been dismissed …
Limbic Mechanisms Controlling Arterial Pressure, Stefanie Roder
Limbic Mechanisms Controlling Arterial Pressure, Stefanie Roder
Digitized Theses
Three series of experiments were done to investigate the output pathways from the central nucleus of the amygdala (ACe) involved in the control of arterial pressure. The first series of experiments provided the first demonstration that the arterial pressure responses elicited during stimulation of ACe were mediated via a direct pathway to the bed nucleus of the stria terminalis (BST). Microinjection of retrograde tract tracers into depressor regions of BST resulted in labelled neurons within predominantly the lateral subdivision of ACe. In addition, iontophoresis of the anterograde tract tracer PHA-L into cardiovascular responsive regions of ACe resulted in fiber and …
Comparative Reproductive Ecology Of Female Buffleheads (Bucephala Albeola) And Barrow's Goldeneyes (Bucephala Islandica) In Central British Columbia, Jonathan Edward Thompson
Comparative Reproductive Ecology Of Female Buffleheads (Bucephala Albeola) And Barrow's Goldeneyes (Bucephala Islandica) In Central British Columbia, Jonathan Edward Thompson
Digitized Theses
Waterfowl (Anseriformes) generally have high energetic costs for reproduction because they lay large, energy-rich eggs. Consequently, many temperate nesting ducks (Anatinae) occupy seasonally productive environments to meet nutritional requirements for egg production. However, ducks in the genus Bucephala often breed in unproductive boreal and montane regions. This study was conducted to investigate nutritional aspects of reproduction in female Buffleheads (Bucephala albeola) and Barrow's Goldeneyes (Bucephala islandica) breeding in central British Columbia.;Diet composition of breeding female Buffleheads and Barrow's Goldeneyes, which consisted primarily of benthic insects, was similar, providing support for the food defense hypothesis as an explanation for evolution of …
Expression Of Sodium Pump Subunit Genes During Mouse Preimplantation Development, Daniel James Macphee
Expression Of Sodium Pump Subunit Genes During Mouse Preimplantation Development, Daniel James Macphee
Digitized Theses
The Na,K-ATPase or sodium pump is an enzyme that is found in most animal cells. The enzyme pumps sodium and potassium ions against their concentration gradients and is hypothesized to be a mediator of fluid accumulation during mouse preimplantation development. The purpose of the present study was to determine, using reverse transcriptase-polymerase chain reaction (RT-PCR), whether all {dollar}\alpha\ (\alpha1,\ \alpha2,\ \alpha3,\ \alpha4{dollar}) and {dollar}\beta\ (\beta1{dollar} and {dollar}\beta{dollar}2) isoforms of the sodium pump were expressed during preimplantation development. Previous work by Watson and Kidder (1988) had shown that the {dollar}\alpha{dollar} subunit was localized to the basolateral domains of mural trophectoderm surrounding …
Role Of Nitric Oxide In Interleukin-2 Therapy-Induced Capillary Leak Syndrome, Amila Orucevic
Role Of Nitric Oxide In Interleukin-2 Therapy-Induced Capillary Leak Syndrome, Amila Orucevic
Digitized Theses
Systemic interleukin-2 (IL-2) therapy has been limited by a major side effect known as "capillary leak syndrome", characterized by retention of extravascular fluid, severe hypotension, and multiple organ system dysfunction. The present study tested the hypothesis that an overproduction of nitric oxide (NO) induced by IL-2 therapy was, at least in part, responsible for this syndrome. Studies were undertaken in healthy or C3-L5 mammary adenocarcinoma bearing C3H/HeJ mice to evaluate whether treatment with inhibitors of NO synthesis (N{dollar}\rm\sp{lcub}G{rcub}{dollar}-Methyl-L-Arginine, NMMA and N{dollar}\rm\sp{lcub}G{rcub}{dollar}-Nitro-L-Arginine methyl ester, L-NAME) can prevent IL-2 induced capillary leakage without compromising the therapeutic benefit of IL-2. Intraperitoneal IL-2 therapy …
P53-Dependent Tumorigenesis And Gene Expression In Mammalian Brain, Meyel Donald Van
P53-Dependent Tumorigenesis And Gene Expression In Mammalian Brain, Meyel Donald Van
Digitized Theses
Astrocytic gliomas are the most common brain cancers of adults. The goal of this research was to examine the molecular genetic determinants of gliomas, with particular emphasis on the tumor suppressor gene P53. First, I studied the frequency, nature and timing of P53 mutations in human gliomas. I observed that glioma-prone families do not have inherited mutations of P53, nor of the Nf1-GRD, Nf2, MTS1, MTS2, or CDK4 genes. However, a high frequency of loss of heterozygosity at chromosome 9p was noted in gliomas that cluster in families. In a second study of low-grade gliomas that progressed to higher grade …
Effects Of Aging And Strength Training On Shortening And Lengthening Muscle Actions In Women, Michelle Marie Porter
Effects Of Aging And Strength Training On Shortening And Lengthening Muscle Actions In Women, Michelle Marie Porter
Digitized Theses
There has been much research on strength changes with aging and the consequent effects of resistance training, however, relatively little is known about lengthening (eccentric; ECC) muscle actions. The purposes of these studies were to develop a method of testing and training plantar (PF) and dorsiflexion (DF) of the ankle in a standing position in order to investigate age-related and training effects on shortening (concentric; CONC) and ECC peak torque (PT) in women.;All subjects were healthy and physically active, and performed all testing and training on an isokinetic dynamometer. Strength testing was performed in both standing and supine positions at …
Central Cardiorespiratory And Peripheral Metabolic Influences On Kinetics Of Oxygen Uptake In Older Humans, Philip David Chilibeck
Central Cardiorespiratory And Peripheral Metabolic Influences On Kinetics Of Oxygen Uptake In Older Humans, Philip David Chilibeck
Digitized Theses
The purpose of this thesis was to determine which factors influence oxygen uptake (VO{dollar}\sb2){dollar} kinetics during exercise in old individuals. These factors may include central {dollar}\rm O\sb2{dollar} delivery (i.e. cardiac output kinetics), the rate of O{dollar}\sb2{dollar} delivery at the exercising muscle, and the rate at which mitochondria can use O{dollar}\sb2.{dollar} Fitness level may also influence VO{dollar}\sb2{dollar} kinetics, by affecting the above factors. These factors were estimated by measuring heart rate (HR) kinetics, muscle capillarization, and citrate synthase (CS) activity, respectively, in groups of old ({dollar}\sim{dollar}65y) and young ({dollar}\sim{dollar}26y) individuals. Capillarization and CS activity of the lateral gastrocnemius were compared to …
Population Dynamics, Production And Resource Allocation In The Zebra Mussel, Dreissena Polymorpha, From The Lower Great Lakes, Canada, Margo Elizabeth Chase
Population Dynamics, Production And Resource Allocation In The Zebra Mussel, Dreissena Polymorpha, From The Lower Great Lakes, Canada, Margo Elizabeth Chase
Digitized Theses
Five populations of Dreissena polymorpha from three lakes (Lake St. Clair, Lake Erie and Lake Ontario) were examined to determine the population dynamics, reproductive cycle, secondary production and the allocation of resources to somatic tissue and shell, and gamete production.;Variation in life history characteristics, such as lifespan, age at first reproduction, maximum size and fecundity were observed both within and among sites, although the among-site variation was greater. The lifespan of D. polymorpha ranged from 2 to {dollar}>{dollar}4 years depending on the lake. The onset of sexual maturity was related to size, not age. Individuals became sexually mature between …
Transcriptional Regulation Of The Urea Cycle Enzyme Genes In The Liver Of Rana Catesbeiana Tadpoles During Spontaneous And Thyroid Hormone-Induced Metamorphosis, Yuqing Eugene Chen
Transcriptional Regulation Of The Urea Cycle Enzyme Genes In The Liver Of Rana Catesbeiana Tadpoles During Spontaneous And Thyroid Hormone-Induced Metamorphosis, Yuqing Eugene Chen
Digitized Theses
The ornithine urea cycle enzymes, carbamyl phosphate synthetase (CPS-1), ornithine transcarbamylase (OTC) and arginase, are liver-specific proteins. Their expression is coordinately activated during the metamorphosis of the Rana catesbeiana tadpole by thyroid hormone (TH), and their presence is critical for the shift of this amphibian from an aquatic, ammonotelic larva into a terrestrial, ureotelic adult. My studies were focused on analyzing the transcriptional regulation of the genes encoding these urea cycle enzymes and determining whether these genes were upregulated directly or indirectly by TH. With this thought in mind, I isolated and characterized the sequences in the promoter regions of …
High Resolution And Wideline Nmr Studies Of Membrane Associated Molecules Involved In Signal Transduction, Alan Cavell Rigy
High Resolution And Wideline Nmr Studies Of Membrane Associated Molecules Involved In Signal Transduction, Alan Cavell Rigy
Digitized Theses
The primary focus of our investigations were centered around understanding the biochemical basis of the early events involved in signal transduction. Our work has involved two novel systems: (1) sphingolipid metabolites and (2) a synthetic peptide representing the transmembrane region of the human epidermal growth factor receptor (hEGFR).;Sphingolipid metabolites have emerged as physical modulators of many signalling pathways, including cell growth regulation, and signal transduction. N,N-({dollar}\sp2{dollar}H{dollar}\sb6{dollar}) -dimethylsphingosine and ({dollar}\sp2{dollar}H{dollar}\sb4{dollar}) -lysogalactosyl ceramide were produced by partial synthesis and studied via wideline {dollar}\sp2{dollar}H NMR, as a function of pH. Spectra of ({dollar}\sp2{dollar}H{dollar}\sb6{dollar}) -dimethylsphingosine identified that the molecule's orientation and behaviour are largely …
Developmental Potential Of Rat Myoblast Lineages, Christopher Louis Pin
Developmental Potential Of Rat Myoblast Lineages, Christopher Louis Pin
Digitized Theses
Skeletal muscle consists of multinucleated fibres which are classified as slow (I) or fast (IIA, IIB, IIX) muscle fibre types which differ in their contractile properties and their expression of contractile protein isoforms. Individual muscles contain characteristic distributions of fibre types which can be identified based on their myosin heavy chain (MyHC) content. While classical studies have demonstrated that the fibre type composition of adult muscles can be altered by extrinsic factors (such as changes in the pattern of innervation), recent studies suggest that different fibre types may be formed from distinct myoblast lineages. The purpose of this study was …
Prenatal Risk Factors For Neurological Abnormalities In The Term And Near Term Neonate, Kathleen Owen O'Connor
Prenatal Risk Factors For Neurological Abnormalities In The Term And Near Term Neonate, Kathleen Owen O'Connor
Digitized Theses
An abnormal neurological examination in the neonatal hospital stay may indicate a transient or permanent neurological disturbance. Abnormal neurological symptoms therefore place an infant at increased risk for subsequent neurological problems.;This case-control study was designed to investigate prenatal and perinatal risk factors for an abnormal neurological examination in the term infant. Infants were born between 1 January, 1988 and 30 June, 1990. The sample consisted of 222 neonates with neurological abnormalities and 666 controls who were free of such problems. Data on the prenatal histories of their mothers, course of the pregnancy, intrapartum events and status of the infant after …
The Influence Of Extremely Low Frequency Magnetic Fields On Cytosolic-Free Calcium In Human Cells, Jeffrey John Carson
The Influence Of Extremely Low Frequency Magnetic Fields On Cytosolic-Free Calcium In Human Cells, Jeffrey John Carson
Digitized Theses
Extremely low frequency (ELF) magnetic fields have been reported to affect Ca{dollar}\sp{lcub}2+{rcub}{dollar}dependent biological processes. Based on these reports, I hypothesized that cystosolic free calcium concentration ({dollar}\lbrack\rm Ca\sp{lcub}2+{rcub}\rbrack\sb{lcub}i{rcub}){dollar} may be influenced by ELF magnetic fields and found an increase in HL-60 cells following exposure {dollar}(P0.09).{dollar} However, experiments with indo-1-loaded Jurkat cells indicated frequency- and field strength-dependent effects on resting and mitogen-stimulated {dollar}\lbrack\rm Ca\sp{lcub}2+{rcub}\rbrack\sb{lcub}i{rcub}{dollar} levels {dollar}(P<0.01).{dollar} The findings show that ELF magnetic fields affect {dollar}\lbrack\rm Ca\sp{lcub}2+{rcub}\rbrack\sb{lcub}i{rcub}{dollar} and may explain some of the reported Ca{dollar}\sp{lcub}2+{rcub}{dollar}-dependent biological effects of ELF magnetic fields.
The In Vitro Studies On Phage Mu Transposase, Zhenguo Wu
The In Vitro Studies On Phage Mu Transposase, Zhenguo Wu
Digitized Theses
Bacteriophage Mu is one of the model systems to study DNA transposition. The availability of an in vitro soluble system greatly facilitates the dissection of the transposition mechanisms at the molecular levels. Several aspects of the in vitro mini-Mu DNA transposition have been dealt with in this thesis.;First, the flanking host DNA sequences on the strand cleavage step mediated by Mu transposase (Mu A protein) was investigated. We found that certain flanking host sequences could inhibit the strand cleavage step without affecting the earlier synapsis step. Furthermore, this cleavage defect could be overcome by the Mu B protein in the …
Neurotransmission Of Visceral Information In The Parabrachial Nucleus Of The Rat, Tarek Saleh
Neurotransmission Of Visceral Information In The Parabrachial Nucleus Of The Rat, Tarek Saleh
Digitized Theses
The parabrachial nucleus (PB) has been shown to be a major relay site for visceral afferents ascending through the ventrobasal thalamus and terminating in the insular cortex. The purpose of these investigations was to determine the neurotransmitter mechanisms in the PB involved in relaying visceral sensory information to the thalamus in the rat. Using electrophysiological techniques in the cloral hydrate anesthetised male Wistar rat, our results show that the visceral pathway from the PB to the thalamus appears to use two neuronal systems, one mediating the relay of specific visceral information via glutamatergic N-methyl D-aspartate (NMDA) receptors in the PB, …
Examination Of Nucleoside Transport In The Mammalian Nervous System, Kenneth William Jones
Examination Of Nucleoside Transport In The Mammalian Nervous System, Kenneth William Jones
Digitized Theses
In rabbit and guinea pig cortical synaptosomes, the nucleoside transport inhibitors nitrobenzylthioinosine and dipyridamole were used to test the hypotheses that the nitrobenzylthioinosine-sensitive and -resistant ({dollar}\sp3{dollar}H) dipyridamole binding sites are associated with the es and ei nucleoside transporters, respectively. In addition, the hypothesis that the es and ei nucleoside transporters in rabbit cortical synaptosomes differ in their selectivity for compounds besides nitrobenzylthioinosine was tested, Nitrobenzylthioinosine-sensitive ({dollar}\sp3{dollar}H) dipyridamole binding and ({dollar}\sp3{dollar}H) nitrobenzylthioinosine binding involved the same site on the es transporter. The relative proportions and inhibitor sensitivities of nitrobenzylthioinosine-resistant ({dollar}\sp3{dollar}H) dipyridamole binding could not be correlated with nitrobenzylthioinosine-resistant nucleoside transport. This, …
Structural Basis For Human Anti-Dna Antibody Specificity, Mahmoud Mahmoudi
Structural Basis For Human Anti-Dna Antibody Specificity, Mahmoud Mahmoudi
Digitized Theses
Anti-DNA antibodies are hallmarks of SLE both in human and inbred lupus prone mice. The role of these antibodies in disease pathogenesis, particularly in glomerulonephritis has been documented. It is now well accepted that the genetic potential to generate anti-DNA antibodies also exists in the normal B cell immune repertoire. This genetic potential has been revealed by detection of anti-DNA antibodies in serum of normal individuals and by immortalizing DNA reactive B cells as hybridomas and as transformed cell lines. There is growing evidence that anti-DNA antibodies from the normal B cell repertoire and disease-associated anti-DNA antibodies share V region …
The Putative Role Of Fatty Acids In The Activation Of Trehalose Synthesis In The Fat Body Of The Cockroach Periplaneta Americana L, Irshad Ali
Digitized Theses
Hypertrehalosemic hormones produced by the corpus cardiacum increase the concentration of several fatty acids in cockroach fat body trophocytes. The phospholipase A{dollar}\sb2{dollar} (PLA{dollar}\sb2){dollar} activator melittin has a similar effect. The fatty acids include palmitic, stearic, oleic and linoleic acid. The hormone mediated release of these fatty acids is blocked by the PLA{dollar}\sb2{dollar} inhibitors mepacrine and {dollar}\rho{dollar}-bromophenacyl bromide. These observations suggest that hormonal release of fatty acids, may be due to the activation of PLA{dollar}\sb2.{dollar};The increase in non-esterified fatty acids appears to be linked to the increase in trehalose efflux since sugar efflux is stimulated by stearic, oleic, linoleic and arachidonic …
Modulation Of Experimental Allergic Encephalomyelitis In The Sjl/J Mice By Cytokines And Anti-Cytokine Antibodies, Trang Thi Duong
Modulation Of Experimental Allergic Encephalomyelitis In The Sjl/J Mice By Cytokines And Anti-Cytokine Antibodies, Trang Thi Duong
Digitized Theses
Immunization with myelin basic protein in complete Freund's adjuvant resulted in only mild chronic-relapsing experimental allergic encephalomyelitis (EAE) with very low incidence in SJL/J mice, and no disease at all in A/J, BALB/c, C3H/HeJ, DBA/2, AKR and NZW mice. However, treatment of mice with anti-IFN{dollar}\gamma{dollar} (aIFN) monoclonal antibody (mAb) at the time of immunization determined severe disease in all those strains except AKR. Similarly, the passive induction of EAE in the SJL/J mice and in two of three resistant strains examined was significantly enhanced by aIFN mAb administration. In the SJL/J mice, administration of anti-interleukin 2 (aIL2) mAb had only …
Image Correlation Spectroscopy: Development And Application To Studies Of Pdgf Receptor Distributions, Paul William Wiseman
Image Correlation Spectroscopy: Development And Application To Studies Of Pdgf Receptor Distributions, Paul William Wiseman
Digitized Theses
Aggregation of macromolecular plasma membrane receptors following ligand binding is hypothesized to be the initial step in signal transduction in cellular systems. Quantitative measurement of receptor distributions on cell surfaces is integral for elucidating the complicated mechanisms involved in transduction of chemical signals across the cells membrane. Scanning fluorescence correlation spectroscopy (S-FCS) has been used successfully to perform quantitative measurements of receptor densities and aggregate sizes. However, these measurements take a great deal of time, and require specialized equipment to implement. Here, image correlation spectroscopy (ICS) is introduced as a novel extension of scanning fluorescence correlation spectroscopy that improves on …
Photosynthetic Acclimation To Light And Temperature: Excitation Pressure And Redox Sensing, Denis Philip Maxwell
Photosynthetic Acclimation To Light And Temperature: Excitation Pressure And Redox Sensing, Denis Philip Maxwell
Digitized Theses
Photosynthetic acclimation to low temperature has been studied extensively. However, since low temperature causes a preferential decrease in the rates of biochemical as compared to photochemical reactions, some alterations brought about by acclimation to low temperature may be the result of the light-harvesting apparatus absorbing more light energy than can be readily dissipated through photosynthesis. This possibility was investigated by using two species of green algae, Chlorella vulgaris and Dunaliella salina and the techniques of chlorophyll a fluorescence, O{dollar}\sb2{dollar}-evolution, protein electrophoresis and Western blotting, high performance liquid chromatography, RNA blot hybridization, and enzyme and metabolite assays. Compared to algae grown …