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West Virginia University

Theses/Dissertations

2014

Genetics

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Identification And Genetic Diversity Of Lake Sturgeon Larvae On Artificial Reefs In The Huron-Erie Corridor, Jamie Marie Marranca May 2014

Identification And Genetic Diversity Of Lake Sturgeon Larvae On Artificial Reefs In The Huron-Erie Corridor, Jamie Marie Marranca

Graduate Theses, Dissertations, and Problem Reports

There are 27 species of sturgeon worldwide and most are currently extinct, endangered or threatened. Lake sturgeon, Acipenser fulvescens, one of the oldest species on the planet, evolved about 136 million years ago, and have had little morphological change, and are endemic to the freshwater lakes and rivers created by receding glaciers of the Laurentian ice sheet. Lake sturgeon have seen immense habitat loss due to human development and industrialization has nearly decimated populations. Changes to water and habitat quality, along with overharvesting, have caused declines to nearly 1% of their original population size. The Huron-Erie Corridor (HEC), includes the …


Utilization Of Fish For Constitutional And Acquired Chromosomal Abnormalities For Diagnostic And Prognostic Purposes, Amy L. Shackelford May 2014

Utilization Of Fish For Constitutional And Acquired Chromosomal Abnormalities For Diagnostic And Prognostic Purposes, Amy L. Shackelford

Graduate Theses, Dissertations, and Problem Reports

ABSTRACT Utilization of FISH for Constitutional and Acquired Chromosomal Abnormalities for Diagnostic and Prognostic Purposes Amy L. Shackelford Fluorescence in-situ hybridization (FISH) is a useful molecular cytogenetics technique for counting chromosomes and identifying specific chromosomal sequences of interest. FISH probe targets include centromeres, single loci, subtelomeres, and telomeres, using DNA or peptide nucleic acid (PNA) probes. FISH probes were used to determine chromosome number, copy loss or gain, and signal size in four studies involving acquired chromosomal changes in malignancy, mosaicism, and aneuploidy in pre- and postnatal constitutional abnormalities. In the first series of experiments, subtelomere probes for 5p and …


Effects Of Culverts On Brook Trout Genetic Diversity, Darren M. Wood May 2014

Effects Of Culverts On Brook Trout Genetic Diversity, Darren M. Wood

Graduate Theses, Dissertations, and Problem Reports

Brook trout (Salvelinus fontinalis) are a species of concern within their native range due to a historical loss of habitat, overfishing, and stocking of non-native salmonids. Road culverts have been recognized as an additional impediment to population persistence as movement between diverse habitat types has been identified as an alternative life-history strategy to maximize spawning and growth. Brook trout were genetically analyzed using a suite of 13 microsatellite loci above 7 culverts with varying levels of passability classified through a physical protocol. While most sites were not found to have losses in genetic diversity, populations above culverts with a high …