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West Virginia University

Theses/Dissertations

2013

Genetics

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Characterization Of Genes Involved In Sumoylation During Embryogenesis In Rainbow Trout (Oncorhynchus Mykiss), Xiaowei Zang Aug 2013

Characterization Of Genes Involved In Sumoylation During Embryogenesis In Rainbow Trout (Oncorhynchus Mykiss), Xiaowei Zang

Graduate Theses, Dissertations, and Problem Reports

SUMOylation is the post-translational modification of proteins by the addition of the small ubiquitin-like modifier (SUMO), which plays an important role in various cellular processes. It has been reported that SUMO and its related proteins are important in diverse reproductive functions such as ovulation, gametogenesis, and embryogenesis. Modification of target proteins by SUMO is an ATP-dependent enzymatic cascade involving three key enzymes: E1 activating enzyme (the heterodimer SAE1-SAE2), E2 conjugating enzyme (UBC9) and several E3 ligating enzymes (PIAS, RanBP2/Nup358 and Pc2). The objectives of this study were to characterize the genes involved in SUMOylation and determine their expression profiles during …


The Evolution Of The Paleopolyploid Genome In Family Salicaceae, Eli Rodgers-Melnick Aug 2013

The Evolution Of The Paleopolyploid Genome In Family Salicaceae, Eli Rodgers-Melnick

Graduate Theses, Dissertations, and Problem Reports

Whole genome duplication is a recurrent phenomenon throughout the history of flowering plants, with at least 1 occuring in the history of each angiosperm lineage. However, following duplication the genome typically undergoes a period of increased structural rearrangements and gene loss, resulting in a diploidized genome with large stretches of syntenic regions resulting from the duplication. This process, termed fractionation, often exhibits biased patterns of gene retention, which appear to correspond with the putative connectedness of genes within biological networks. In this thesis, I explore the extent to which the same biases appear in duplicate genes from the most recent …