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Modulation Of Gene Expression And Dna Adduct Formation By Chlorophyllin In Human Mammary Cells Exposed To Benzopyrenes, Kaarthik John
Modulation Of Gene Expression And Dna Adduct Formation By Chlorophyllin In Human Mammary Cells Exposed To Benzopyrenes, Kaarthik John
Graduate Theses, Dissertations, and Problem Reports
This study investigated metabolic activation by benzopyrenes (BP and B[e]P) in human mammary cells and modulation by chlorophyllin (CHL, a chemopreventive agent). Among 6 NHMEC strains monitored using microarrays, 54 genes were up-regulated and 11 down-regulated by signal log ratio (SLR) ≥ 1.5 on treatment with BP alone. Pre CHL + post BP+CHL treatment up-regulated the expression of 129 genes and down-regulated those of 35 genes by SLR ≥ 1.5. Studies on CYP1 gene induction and BP-DNA adduct formation among 20 NHMECs revealed wide inter-individual variations both in the induction (3-96-fold for CYP1A1 and 4-43-fold for CYP1B1, respectively) and modulation …
Genetic Analysis Of Rainbow Trout (Oncorhynchus Mykiss): Strain Identification Via Microsatellites And Analysis Of Expressed Sequence Tags In Intestine, Liver, Kidney, And Ovary, Amanda B. Stewart
Graduate Theses, Dissertations, and Problem Reports
Implementation of modern fishing techniques and hatchery technologies necessitates use of genetics in management of fishery stocks. Genetic analysis of rainbow trout, Oncorhynchus mykiss, will assist development of superior strains of a valuable aquacultured species. This study was to (1) develop polymorphic microsatellite markers to examine for variation among rainbow trout strains and develop an identification panel of microsatellites to distinguish individual strains, and (2) to identify novel genes and estimate relative gene expression profiles (expressed sequence tags from rainbow trout liver, intestine, kidney, and ovary non-normalized libraries). Thirty-seven microsatellites were identified by screening 576 clones from TAGA and ATG-repeat …