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Combinatory Effects Of The Bioflavonoid Apigenin With Chemotherapeutic Drugs On Prostate, Colon, And Lung Cancer Cell Lines, Lesly Anne J. Lopez
Combinatory Effects Of The Bioflavonoid Apigenin With Chemotherapeutic Drugs On Prostate, Colon, And Lung Cancer Cell Lines, Lesly Anne J. Lopez
Graduate Theses, Dissertations, and Problem Reports
Lung, prostate, and colon cancer are among the most commonly diagnosed cancers in Americans. Current chemotherapeutic regimens generally have toxic side effects leading to the death of normal cells. Polyphenols are naturally occurring compounds that can be found in fruits, vegetables and herbs. Apigenin is a polyphenolic compound that has been shown to have antioxidant and anti-inflammatory effects. In this study, apigenin was shown to inhibit the proliferation of several cancer cell lines. The combination of 15 uM apigenin and the chemotherapeutic drugs including doxorubicin, etoposide, mitomycin C, and 5-fluorouracil significantly decreased (P<0.05) the IC50 to inhibit the proliferation of prostate cancer cell lines PC-3 and DU-145 more than the chemotherapeutic drugs alone. Similarly, the addition of apigenin greatly enhanced the sensitivity of colon cancer cell lines HCT-116 and colo-205 and lung cancer lines A549 and H460 to doxorubicin, mitomycin C, 5-fluorouracil, and cisplatin. We analyzed sensitivity via cell death and cell proliferation assays.
Stationary Phase Induction Of Rpos In Enteric Bacteria, Matthew Louis Hirsch
Stationary Phase Induction Of Rpos In Enteric Bacteria, Matthew Louis Hirsch
Graduate Theses, Dissertations, and Problem Reports
In enteric bacteria, stress adaptation is mediated by the RpoS protein, one of several sigma-factors that, in association with RNA polymerase, collectively allow a tailored transcriptional response to environmental cues. Stress stimuli including low temperature, osmotic shock, and starvation all result in a substantial increase in RpoS abundance. Perhaps the most pronounced affect is observed during growth to stationary phase (SP) in rich medium. The mechanism of regulation depends on the specific signal, but may occur at the level of transcription, translation, protein activity or targeted proteolysis. In both Escherichia coli and Salmonella enterica cultured in rich undefined medium, the …
Drosophila Melanogaster Protein Kinase Ck2 Interacts With And Phosphorylates The Neurogenic Repressor M8 Resulting In The Production Of A Novel Eye Phenotype, Regina L. Trott
Graduate Theses, Dissertations, and Problem Reports
Protein kinase CK2 is a serine/threonine protein kinase ubiquitously expressed in eukaryotic organisms. The enzyme is composed of 2 alpha (catalytic) subunits and 2 beta (regulatory) subunits. The minimum consensus site for CK2 phosphorylation is S/TXXD/E, where "X" is any non-basic amino acid. Substrates so far identified include proteins associated with cell cycle regulation and development.;In Drosophila melanogaster, the Notch pathway activates members of the Enhancer-of-split Complex [E(spl)-C]. m8, which is a member of the E(spl)-C, was found to interact with and be phosphorylated by CK2. The physiological affects of CK2 phosphorylation were studied in vivo through the Gal4-UAS system. …
Graph Algorithms For The Haplotyping Problem, Yunkai Liu
Graph Algorithms For The Haplotyping Problem, Yunkai Liu
Graduate Theses, Dissertations, and Problem Reports
Evidence from investigations of genetic differences among human beings shows that genetic diseases are often the results of genetic mutations. The most common form of these mutations is single nucleotide polymorphism (SNP). A complete map of all SNPs in the human genome will be extremely valuable for studying the relationships between specific haplotypes and specific genetic diseases. Some recent discoveries show that the DNA sequence of human beings can be partitioned into long blocks where genetic recombination has been rare. Then, inferring both haplotypes from chromosome sequences is a biologically meaningful research topic, which has compounded mathematical and computational problems.;We …
Functional Analysis Of Atrpd3b, A Rpd3-Type Histone Deacetylase, In Arabidopsis, Lin Zhang
Functional Analysis Of Atrpd3b, A Rpd3-Type Histone Deacetylase, In Arabidopsis, Lin Zhang
Graduate Theses, Dissertations, and Problem Reports
Histone acetylation is modulated through the action of histone acetyltransferases and deacetylases, which play key roles in the regulation of eukaryotic gene expression. The RPD3 group of histone deacetylases constitutes the first class of histone deacetylases (HDACs) in eukaryotes.;In yeast and mammalian cells, it was found that RPD3 histone deacetylases are involved in the ageing and development. In contrast much less is known about the function of RPD3s in plant. Here I studied AtRPD3B, a RPD3-type histone deacetylase in Arabidopsis thaliana, to define its role in plant development and signal transduction pathways. Using the AtRPD3B: GUS transgenic Arabidopsis plants, I …