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Green Tea Antioxidants Inhibition Of Oxidation And Mutation, Paul Stout Mcconnell
Green Tea Antioxidants Inhibition Of Oxidation And Mutation, Paul Stout Mcconnell
Graduate Theses, Dissertations, and Problem Reports
Green tea is a complex mixture containing several major potent antioxidants e.g., flavins and/or polyphenols. These antioxidants in green tea may react directly or indirectly with strong oxidizers e.g. peroxynitrite or its constituents (superoxide or nitric oxide). Green tea antioxidants can destroy the oxidants. Based on a simple chemical interaction of peroxynitrite (OONO-) and luminol, blue light is produced upon oxidation. It was shown that green tea and constituents have light inhibitory capabilities. In order to show the possible beneficial effects of green tea with DNA, plasmids were chosen to determine whether or not green tea was also capable of …
Higher Order Chromatin Degradation Induced By Hydrogen Peroxide In Glial Cells, Raymond Mouzannar
Higher Order Chromatin Degradation Induced By Hydrogen Peroxide In Glial Cells, Raymond Mouzannar
Graduate Theses, Dissertations, and Problem Reports
This study addresses the genotoxicity of oxidative stress. We induced oxidative stress in cultured rat oligodendrocytes by exposure to hydrogen peroxide (H2O2). Intact genomic DNA was isolated by agarose embedding and its fragmentation was assessed by field inversion gel electrophoresis (FIGE). To detect single strand fragmentations, the DNA was digested with S1 nuclease before FIGE. We found that the exposure of oligodendrocytes to pathological concentrations of H2O2, induces a rapid digestion of nuclear chromatin in a pattern consistent with higher order chromatin degradation (HOCD). HOCD, the hallmark of programmed cell death (PCD), proceeds through single strand scissions that accumulate at …
Genetic Evaluation Of Cytochrome P450 Expression In Smoking And Nonsmoking Women, Satya Vijayanand Vadlamuri
Genetic Evaluation Of Cytochrome P450 Expression In Smoking And Nonsmoking Women, Satya Vijayanand Vadlamuri
Graduate Theses, Dissertations, and Problem Reports
1. Regiospecific expression of CYP1A1 and CYP1B1 in human uterus of nonsmokers. The goal of this study was to investigate the expression of these isoforms in different regions of the human uterus. Expression was determined in the endometrium, endocervix and squamous (exocervix) regions from six nonsmoking women by using RT-PCR. The transcripts encoding for CYP1A1 were significantly higher (p < 0.05) in the squamous (exocervix) region compared to other areas. However the expression of CYP1B1 was significantly higher (p < 0.05) in the endometrium. CYP1B1 expression appeared to be extremely low in a woman in the secretory stage of the menstrual phase, relative to the endometrium of the other patients who were all in the proliferative stage at hysterectomy. It appears that variability in the expression of these isoforms may be responsible for the differential susceptibility to cancer in women.;2. Influence of menstrual phase and smoking status on expression of CYP1A1 and CYP1B1 in different regions of the uterus. The goal of this study was to investigate the expression of these isoforms in different regions of the uterus from smokers and nonsmokers. These results indicate that CYP1A1 and CYP1B1 are expressed variably in different regions of the human uterus. The expression of CYP1B1 may be susceptible both to hormonal control and cigarette smoking. The variability in the expression of these isoforms may be responsible for the differential susceptibility to cancer in some women.;3. The effect of menstrual phase on CYP3A expression in human endometrium. The CYP3A family consists of four closely related proteins. Of these, CYP3A3/4 is the most abundant P450 isoform and is responsible for metabolizing estrogen as well as a variety of therapeutic agents, whereas CYP3A7, the fetal liver form, is involved in the biosynthesis of estriol. The goal of this study was to evaluate the expression of these two major CYP3A isoforms in human uterine tissue during the proliferative (PROL) and secretory (SEC) phases of the menstrual cycle. Expression of CYP3A7 in endometrium appeared to be several fold higher (∼10-fold) in the proliferative phase compared to the secretory phase. CYP3A4 expression was comparable between the two phases of the menstrual cycle. The RTPCR results indicated differential expression of CYP3A7 in various phases of the menstrual cycle. These results indicate that CYP3A isoforms may be subject to hormonal regulation during the menstrual cycle in endometrium of premenopausal women. (Abstract shortened by UMI.).