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Role Of Mir-155 And Mir-146a In Mast Cell Function, Amina Abdul Qayum
Role Of Mir-155 And Mir-146a In Mast Cell Function, Amina Abdul Qayum
Theses and Dissertations
Mast cells are resident immune cells abundantly found in the tissue at the host-environment interface, where they play a critical role in inflammatory allergic responses. Mast cell responses may be regulated by the cytokine milieu at the site of inflammation. Recent studies have revealed microRNAs to be important in altering cytokine signaling in immune cells. Here, we demonstrate for the first time that IL-10 and IL-33 induce miR-155 and miR-146a, respectively, to alter mast cell functions. We report that IL-10 enhanced IgE induced activation of mast cells. IL-10 effects are dependent on Stat3 activation, which elicits miR-155 expression, resulting in …
Self-Forgiveness In Romantic Relationships: The Impact On The Perpetrator And The Relationship, Jaclyn M. Moloney
Self-Forgiveness In Romantic Relationships: The Impact On The Perpetrator And The Relationship, Jaclyn M. Moloney
Theses and Dissertations
Two studies were conducted to determine how self-forgiveness and other perpetrator reactions influence the perpetrator and the victim after a romantic relationship transgression. Study 1 used a longitudinal design to determine how guilt and shame predicted the trajectory of self-forgiveness, self-excusing, and self-punishing in participants who had recently been the perpetrator of a romantic relationship transgression.
Those experiencing higher guilt at baseline had higher self-forgiveness starting out and those lower on guilt starting out had a greater change in self-forgiveness. Those experiencing more guilt at baseline experienced less change in self-forgiveness over time. Shame was not significantly related to self-forgiveness …
Heme Oxygenase 1 Expression After Traumatic Brain Injury And Effect Of Pharmacological Manipulation On Functional Recovery., Nicholas H. Russell
Heme Oxygenase 1 Expression After Traumatic Brain Injury And Effect Of Pharmacological Manipulation On Functional Recovery., Nicholas H. Russell
Theses and Dissertations
Traumatic Brain Injury (TBI) is an increasingly diagnosed constellation of injuries derived from acute mechanical trauma to the brain. With the rise of advanced neuroimaging techniques recent focus has oriented primarily towards the mild-moderate range of TBI which previously was missed diagnostically. Characteristically, these advances have shown increasing areas of micro-hemorrhage in susceptible areas of the brain and to date there are no treatment modalities targeting micro-hemorrhages or their sequelae. This dissertation explores the effects of the resulting heme processing response in the days following injury with a particular focus on inducing early heme clearance from the parenchyma using a …
Nitrosative Stress Sensing In Porphyromonas Gingivalis: Structure And Function Of The Heme Binding Transcriptional Regulator Hcpr, Benjamin R. Belvin
Nitrosative Stress Sensing In Porphyromonas Gingivalis: Structure And Function Of The Heme Binding Transcriptional Regulator Hcpr, Benjamin R. Belvin
Theses and Dissertations
Porphyromonas gingivalis, a Gram negative anaerobe implicated in the progression of periodontal disease, is capable of surviving and causing infection despite high levels of reactive nitrogen species found in the oral cavity due to its efficient nitrosative stress response. HcpR is an important sensor-regulator that plays a vital step in the initiation of the nitrosative stress response in many Gram negative anaerobic bacteria. We employ a combination of X-ray crystallography, SAXS, resonance Raman spectroscopy, UV-Vis spectroscopy, and molecular biology techniques to better understand this key regulator. Knockout of the hcpR gene in W83 P. gingivalis results in the inability of …
The Institute Of New Feelings: Plastic Identities And Imperfect Surfaces, Weijian Zhou
The Institute Of New Feelings: Plastic Identities And Imperfect Surfaces, Weijian Zhou
Theses and Dissertations
Digital media are moldable spaces where an image is simultaneously a thought. This instance and flexibility enables digital existences to be malleable, transformative, situational, and unstable. They are plastic images. Video games generate digital bodies that are a fusion of subjectivities and cybernetic simulations, in a perceivable and ambiguous process. Such bodies are extensions of ourselves, being girlish, imperfect, unfinished and happening—digesting and emitting clusters of feelings, regardless of our biological gender and age. The performative experience of play is progressively departing from spectacle, gambling and competition, and increasingly shifting towards an emotional journey of alternate realities, spreading subjectivities into …
Preparation And Evaluation Techniques Of Porous Materials And Mixed Matrix Membranes For Targeted Co2 Separation Applications, Tsemre Tessema
Preparation And Evaluation Techniques Of Porous Materials And Mixed Matrix Membranes For Targeted Co2 Separation Applications, Tsemre Tessema
Theses and Dissertations
The use of porous sorbents for physisorptive capture of CO2 from gas mixtures has been deemed attractive due to the low energy penalty associated with recycling of such materials. Porous organic polymers (POPs) have emerged as promising candidates with potential in the treatment of pre- and post- fuel combustion processes to separate CO2 from gas mixtures. Concurrently, significant advances have been made in establishing calculation methods that evaluate the practicality of porous sorbents for targeted gas separation applications. However, these methods rely on single gas adsorption isotherms without accounting for the dynamic gas mixtures encountered in real-life applications. …
Edge-Transitive Bipartite Direct Products, Cameron M. Crenshaw
Edge-Transitive Bipartite Direct Products, Cameron M. Crenshaw
Theses and Dissertations
In their recent paper ``Edge-transitive products," Hammack, Imrich, and Klavzar showed that the direct product of connected, non-bipartite graphs is edge-transitive if and only if both factors are edge-transitive, and at least one is arc-transitive. However, little is known when the product is bipartite. This thesis extends this result (in part) for the case of bipartite graphs using a new technique called "stacking." For R-thin, connected, bipartite graphs A and B, we show that A x B is arc-transitive if and only if A and B are both arc-transitive. Further, we show A x B is edge-transitive only …
The Fractured Memory Of A Mind’S Eye, Russell G. White
The Fractured Memory Of A Mind’S Eye, Russell G. White
Theses and Dissertations
The work I create is informed by questioning reality/identity, the fractalizing planes
of existence our essence occupies, and the artifacts of memory experience navigating
through space time. While existing in this realm of oversaturated media and neon
glow, I question the effects of pervasive data systems overloading or programming the
mental software we possess. My work includes humor as a means of exploring these
conventions while also displaying psychedelic surrealist imagery to help break away
from the conscious prison this existence births our concept apparatuses within.
Prevalence And Clinical Characteristics Of Teeth Extracted With A Diagnosis Of Cracked Tooth: A Retrospective Study, Riley B. Sturgill
Prevalence And Clinical Characteristics Of Teeth Extracted With A Diagnosis Of Cracked Tooth: A Retrospective Study, Riley B. Sturgill
Theses and Dissertations
The body of knowledge that exists regarding cracked teeth is limited. The purpose of this study was to determine the prevalence of cracks among extracted teeth. This retrospective longitudinal cohort study included patients of the Virginia Commonwealth University School of Dentistry that underwent extraction procedures over a 6 year period. The sample consisted of 20,408 patients and 40,870 teeth. Statistical analysis software was used to identify diagnoses of a crack, fracture, or split tooth prior to extraction of the tooth by analyzing the Electronic Health Record (EHR) (axiUm™, Version 6.03.03.1035, Exan Corporation, Vancouver, BC, Canada). There were 3,228 teeth …
Sleep Disturbances And Depression: The Role Of Genes And Trauma, Mackenzie J. Lind
Sleep Disturbances And Depression: The Role Of Genes And Trauma, Mackenzie J. Lind
Theses and Dissertations
Sleep disturbances and insomnia are prevalent, with around 33% of adults indicating that they experience at least one main symptom of insomnia, and bidirectional relationships exist with common psychopathology, particularly major depressive disorder (MDD). However, genetic and environmental (e.g., traumatic event exposure) contributions to the etiology of these phenotypes are not yet well understood. A genetically informative sample of approximately 12,000 Han Chinese women aged 30-60 (50% with recurrent MDD) was used to address several gaps within the sleep literature. Sleep disturbances were assessed in all individuals using a general item addressing sleeplessness (GS). A sleep within depression sum score …
Advocate Or Traditional Bureaucrat: Understanding The Role Of Esl Supervisors In Shaping Local Education Policy Toward Immigrant Communities, Grant E. Rissler
Advocate Or Traditional Bureaucrat: Understanding The Role Of Esl Supervisors In Shaping Local Education Policy Toward Immigrant Communities, Grant E. Rissler
Theses and Dissertations
As recent immigrants seek a productive and dignified life in “new immigrant destinations” that have little historical experience with immigration, public education systems serve a key function in immigrant integration efforts. In a federal system increasingly focused on accountability, a crucial sub-set of education policy and local responsiveness to immigration is English language instruction and services for Limited English Proficient (LEP) students and parents.
In such contexts, the role that local bureaucrats play, and whether they actively represent the interests of the newfound diversity of community members, are crucial questions if strongly held American ideals of social equity and equal …
Implementing Integrated Care In Family Medicine: Description And Outcomes In An Underserved Population, Zach Radcliff
Implementing Integrated Care In Family Medicine: Description And Outcomes In An Underserved Population, Zach Radcliff
Theses and Dissertations
Introduction: Family physicians provide access to medical and behavioral healthcare for many underserved populations. Integrating behavioral health clinicians into primary care practices has been proposed as one of the most effective ways to increase access to necessary behavioral health services for many Americans. Integrated behavioral healthcare (IBHC) has begun to be implemented in family medicine practices but there is limited research examining the impact for patients and clinic staff. This study begins to fill this gap in the literature by examining the effects of implementing integrated behavioral healthcare in an urban family medicine clinic in a medically underserved area.
Objective: …
Understanding The Expressive Cartoon Drawings Of A Student With Autism Spectrum Disorder, Michael S. Collins
Understanding The Expressive Cartoon Drawings Of A Student With Autism Spectrum Disorder, Michael S. Collins
Theses and Dissertations
This study focuses on the highly expressive comic drawings of Amy, a child with autism. This study connects larger fields of research: the study of how people with autism spectrum disorder [ASD] process faces and emotions; and, research about artists with ASD. Amy's understanding of emotion was analyzed by asking her to view and identify humans and cartoon characters expressing different emotions. Her ability to illustrate emotion is tested by asking her to respond to various drawing prompts. The study concluded that Amy has difficulty identifying the emotions of humans and cartoons, but she does have the ability to illustrate …
Health Aid In Africa: Placement, Service Utilization, And Benefit, Carrie Dolan
Health Aid In Africa: Placement, Service Utilization, And Benefit, Carrie Dolan
Theses and Dissertations
While the health sector has attracted significant foreign aid, evidence on the effectiveness of this support is mixed. This dissertation examines the allocation of health aid within the context of placement, service utilization, and benefit. The first paper examined the sub-national allocation of Chinese development aid projects across Africa. I determined how political preferencing of Chinese aid specifically, allocating aid to the birth region of the current political leader differs across sectors such as health, education, and transportation. I find some evidence that aid, more broadly defined, is subject to political preferencing in recipient countries, which could potentially limit its …
Ain’T I Black Enough?: Anxiety Related To The Questioning Of One’S Group Membership, Michelle Stith
Ain’T I Black Enough?: Anxiety Related To The Questioning Of One’S Group Membership, Michelle Stith
Theses and Dissertations
During late adolescence and emerging adulthood, individuals undergo identity formation (Erikson, 1956). During this period, individuals may be insecure about their group memberships (Myers & DeWall, 2015). Subcategorizations have developed among Black people in America resulting from their traumatic history (Blair, Judd, Sadler, & Jenkins, 2002; Levingston & Brewer, 2002). This subcategorization creates in-group/out-group dichotomies. In order to increase one’s self-image, Social Identity Theory (Tajfel & Turner, 1979) poses that individuals attempt to enhance the image of their in-group. This study aimed to determine whether a person feels anxiety due to fear of rejection from the in-group when challenged on …
Weighted Quantile Sum Regression For Analyzing Correlated Predictors Acting Through A Mediation Pathway On A Biological Outcome, Bhanu M. Evani
Weighted Quantile Sum Regression For Analyzing Correlated Predictors Acting Through A Mediation Pathway On A Biological Outcome, Bhanu M. Evani
Theses and Dissertations
Abstract
Weighted Quantile Sum Regression for Analyzing Correlated Predictors Acting Through a Mediation Pathway on a Biological Outcome
By
Bhanu M. Evani, Ph.D.
A thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University.
Virginia Commonwealth University, 2017.
Major Director: Robert A. Perera, Asst. Professor, Department of Biostatistics
This work examines mediated effects of a set of correlated predictors using the recently developed Weighted Quantile Sum (WQS) regression method. Traditionally, mediation analysis has been conducted using the multiple regression method, first proposed by Baron and Kenny (1986), which has since …
Using Restoration-Oriented Coping And The Dual Process Model With Bereaved Undergraduates, Farah J. Aslanzadeh
Using Restoration-Oriented Coping And The Dual Process Model With Bereaved Undergraduates, Farah J. Aslanzadeh
Theses and Dissertations
Approximately 60% of college seniors lost at least one family member or friend since beginning college (Cox, Dean, & Kowalski, 2015). Research reveals that bereaved students are more likely than their nonbereaved peers to struggle with academic problems and attrition (Cousins, Servaty-Seib & Lockman, 2017), highlighting the importance of identifying protective factors for this group of individuals. Researchers have identified restoration-oriented coping as a helpful coping mechanism in other samples (Caserta & Lund, 2007; Caserta, Lund, Utza, & de Vries, 2009). Despite qualitative evidence suggesting bereaved undergraduates often employ restoration-oriented coping, no research has formally assessed the effects of restoration-oriented …
A Multi-Method Exploration Of The Genetic And Environmental Risks Contributing To Tobacco Use Behaviors In Young Adulthood, Elizabeth K. Do
A Multi-Method Exploration Of The Genetic And Environmental Risks Contributing To Tobacco Use Behaviors In Young Adulthood, Elizabeth K. Do
Theses and Dissertations
Tobacco use remains the leading preventable cause of morbidity and mortality in both the United States and worldwide. Twin and family studies have demonstrated that both genetic and environmental factors are important contributors to tobacco use behaviors. Understanding how genes, the environment, and their interactions is critical to the development of public health interventions that focus on the reduction of tobacco related morbidity and mortality. However, few studies have examined the transition from adolescent to young adulthood – the time when many individuals are experimenting with and developing patterns of tobacco use. This dissertation thesis seeks to provide a comprehensive …
Walking In Beauty: Responsive And Responsible Health And Healing Among Virginia American Indian People, Amy J. Prorock-Ernest
Walking In Beauty: Responsive And Responsible Health And Healing Among Virginia American Indian People, Amy J. Prorock-Ernest
Theses and Dissertations
Little is systematically known about the collective health and well-being of Virginia American Indian people. This study sought to explore the meaning of health and healing among Virginia American Indian people in the context of a reservation-based, non-federally funded health clinic. Using an emergent approach to qualitative research grounded in a constructivist inquiry paradigm and guided by Indigenous research principles, a total of 24 in-depth, semi-structured interviews were conducted with 17 American Indian service-users of the Clinic. Through an inductive thematic analysis of participant stories, a framework for understanding responsive and responsible health and healing was derived. The framework includes …
Theatrical Texts And Contexts: Poe And Hawthorne’S Fictional Women, Savannah M. Singletary
Theatrical Texts And Contexts: Poe And Hawthorne’S Fictional Women, Savannah M. Singletary
Theses and Dissertations
Edgar Allen Poe and Nathaniel Hawthorne are arguably two of the most highly read and heavily debated nineteenth-century antebellum authors in America. Their writings fascinate readers, while their character depictions, particularly their characterizations of fictional women, prompt intense academic debate. This thesis examines the previously less-studied historical developments surrounding Poe and Hawthorne in the antebellum era that shaped their approach to writing fiction. In particular, this study scrutinizes the effects of the development of a newly popular art form, ballet, the ascendency of female authorship, and the impact of American theatrical reform upon antebellum authors’ authorial faculties, especially Hawthorne and …
Person-Centered Treatment To Optimize Psychiatric Medication Adherence, Natalie Bareis
Person-Centered Treatment To Optimize Psychiatric Medication Adherence, Natalie Bareis
Theses and Dissertations
Objectives: Adherence to psychotropic medication is poor among individuals with bipolar disorder (BD). To understand treatment experiences and associated adherence among these individuals, we developed a novel construct of Clinical Net Benefit (CNB) using psychiatric symptoms, adverse effects and overall functioning assessments. We tested whether adherence differed across classes of CNB, whether individuals transitioned between classes over time, and whether these transitions were differentially associated with adherence.
Methods: Data come from individuals aged 18+ during five years of the Systematic Treatment Enhancement Program for Bipolar Disorder (STEP-BD). Latent class analysis identified groups of CNB. Latent transition analysis determined probabilities of …
Discovery And Characterization Of Bile Acid And Steroid Metabolism Pathways In Gut-Associated Microbes, Spencer Harris
Discovery And Characterization Of Bile Acid And Steroid Metabolism Pathways In Gut-Associated Microbes, Spencer Harris
Theses and Dissertations
The human gut microbiome is a complex microbial ecosystem residing in the lumen of our gastrointestinal tract. The type and amounts of microbes present in this ecosystem varies based on numerous factors, including host genetics, diet, and environmental factors. The human gut microbiome plays an important role in normal host physiological functions, including providing energy to colonocytes in the form of short-chain fatty acids. However, gut microbial metabolites have also been associated with numerous disease states. Current tools for analyzing the gut microbiome, such as high-throughput sequencing techniques, are limited in their predictive ability. Additionally, “-omic” approaches of studying the …
Impact Of School-Based Sex Education On College Students’ Rape Myth Acceptance: An Exploratory Analysis, Erika Carpenter
Impact Of School-Based Sex Education On College Students’ Rape Myth Acceptance: An Exploratory Analysis, Erika Carpenter
Theses and Dissertations
Research indicates nearly one-fourth of college women fall victim to sexual assault (Cantor et. al., 2015). Two predictors of high proclivity to rape are endorsement of rape myths and adherence to traditional gender norms (King & Roberts, 2011). Additionally, research shows school-based sex education in the United States presents gender and sexual norms in troubling ways that disproportionately harm women (Kendall, 2013). However, research on sexual assault and rape myths have not examined the impact school-based sex education has on rape supportive attitudes. This study aimed to bridge that gap by using original survey data from undergraduate students at a …
Contribution Of Asc-Inflammasome To Vascular Endothelial Dysfunction: Role Of Rna Receptor Rig-I, Ashley Pitzer
Contribution Of Asc-Inflammasome To Vascular Endothelial Dysfunction: Role Of Rna Receptor Rig-I, Ashley Pitzer
Theses and Dissertations
Retinoic acid-inducible gene-I (RIG-I) is a putative RNA helicase and recently identified as a cytosolic RNA receptor in mammalian cells. The role of RIG-I in the regulation of vascular function under physiological and pathological conditions is unknown. Recent studies have shown that the inflammasome serves as a crucial initiator of cytokine-mediated inflammation mediating the pathogenesis of cardiovascular disease. The present study tested whether RIG-I activation triggers inflammasome formation and subsequent cytokine-mediated inflammation in the endothelium of mice coronary arteries. Using both genetic and pharmacological interventions of the RIG-I inflammasome, we first characterized whether specific activation of RIG-I via 3pRNA transfection …
A Biorelevant In Vitro Model To Characterize In Vivo Release Of Bone Morphogenetic Protein-2 (Rhbmp-2), Deblina Biswas
A Biorelevant In Vitro Model To Characterize In Vivo Release Of Bone Morphogenetic Protein-2 (Rhbmp-2), Deblina Biswas
Theses and Dissertations
Biorelevant in vitro release/dissolution tests are designed to predict the in vivo behavior of a drug and are crucial in understanding its in vivo performance. Currently, there is no standardized compendial in vitro release testing methods or regulatory guidance’s for release/dissolution testing of implants due to their complex physiological locations.Furthermore, existing compendial methods do not capture the local release profile of ‘novel’ parenterals in physiological low fluid volume surrounding areas.
Long acting and in situ forming implants with orthobiologic proteins and peptides have increased over the past few decades due to a better understanding of genetic engineering. One of these …
Comparing The Structural Components Variance Estimator And U-Statistics Variance Estimator When Assessing The Difference Between Correlated Aucs With Finite Samples, Anna L. Bosse
Theses and Dissertations
Introduction: The structural components variance estimator proposed by DeLong et al. (1988) is a popular approach used when comparing two correlated AUCs. However, this variance estimator is biased and could be problematic with small sample sizes.
Methods: A U-statistics based variance estimator approach is presented and compared with the structural components variance estimator through a large-scale simulation study under different finite-sample size configurations.
Results: The U-statistics variance estimator was unbiased for the true variance of the difference between correlated AUCs regardless of the sample size and had lower RMSE than the structural components variance estimator, providing better type 1 error …
Bridging The Empathy Gap: Effects Of Brief Mindfulness Training On Helping Outgroup Members In Need, Daniel R. Berry
Bridging The Empathy Gap: Effects Of Brief Mindfulness Training On Helping Outgroup Members In Need, Daniel R. Berry
Theses and Dissertations
Witnessing others in need can be felt similarly to experiencing it oneself (empathy) and motivates assistance of those in need (prosocial action). It is well-documented that empathy can occur automatically, but when those in need are not members of a social ingroup, empathy and prosocial action are undermined. One major ingroup—outgroup division in American and in other countries is based on race. Although most condemn racial discrimination, empathy and prosocial action are often lower, however unintentionally, in interracial contexts. In light of this empathy gap, it is important to identify psychological factors that could bolster empathy and prosocial action toward …
Immersive Theater & The Physical Narrative, Lauren Prisco
Immersive Theater & The Physical Narrative, Lauren Prisco
Theses and Dissertations
Immersive Theater is a form of experimental theater that places spectators at the heart of the created work, by removing them from the constraint of static seats and instead encouraging them to explore an installed environment as a way of understanding the narrative. This thesis explores how Interior Design directly enhances a performance by creating spaces that challenge a spectator’s physical understanding of a narrative.
Role Of Translation Initiation In Regulation Of Epithelial Junctions And Cell Motility, Fahda Fawaz Alsharief
Role Of Translation Initiation In Regulation Of Epithelial Junctions And Cell Motility, Fahda Fawaz Alsharief
Theses and Dissertations
The integrity and barrier properties of intestinal epithelium are determined by specialized adhesive structures known as intercellular junctions; composed of adherens junctions (AJs), tight junctions (TJs) and focal adhesions that mediate cell-cell and cell matrix interactions, respectively. These two types of epithelial cell adhesions regulate each other during disruption and restitution of the epithelial barrier. Inflammatory cytokines such as interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) are elevated during intestinal inflammation. The most notable effects of IFNγ and TNFα on intestinal epithelial homeostasis involve disruption of apical junctions and attenuation of cell migration. Although molecular mechanisms underlying these …
Big Data Visualization Platform For Mixed Reality, Aliakbar Panahi
Big Data Visualization Platform For Mixed Reality, Aliakbar Panahi
Theses and Dissertations
The visualization of data helps to provide faster and deeper insight into the data. In this work, a system for visualizing and analyzing big data in an interactive mixed reality environment is proposed. Such a system can be used for representing different types of data such as temporal, geospatial, network graph, and high dimensional. Also, an implementation of this system for four different data types are created. The data types include network data, volumetric data, high dimensional, and spectral data for different mixed reality devices such as Microsoft HoloLens, Oculus Rift, Samsung Gear VR, and Android ARCore were created. It …