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X-Ray Microanalysis In Cryosections Of Natively Frozen Paramecium Caudatum With Regard To Ion Distribution In Ciliates, M. Schmitz, R. Meyer, K. Zierold

Scanning Electron Microscopy

Cells of Paramecium caudatum were shock-frozen without pretreatment for cryoultramicrotomy and freeze-dried for subsequent X-ray microanalysis. Na, Mg, P, S, Cl, K, and Ca were detected in different amounts in several subcellular compartments. In particular, calcium was localized below the cell surface (pellicle). Trichocysts were found to contain significant amounts of Na in their base but not in the tip. Na, Mg, P, S, Cl, K, Ca were found in electron dense deposits within the lumen of the contractile vacuole. A small K concentration was found in the cytoplasm and in the mitochondria. X-ray microanalysis of the element distribution in …

Scanning Electron Microscopy In Retinal Research, Bessie Borwein

Scanning Electron Microscopy

The literature on scanning electron microscopy (SEM) pertaining to the retina has been surveyed and described.

The first two papers on SEM and retina appeared in 1969. Most of the earlier studies concentrated on descriptions [by SEM alone, or with light microscopy (LM) and transmission electron microscopy (TEM)] of the appearance of various retinae and retinal cells [fish, newt, primates, rodents and rabbits, bullfrog]; or embryology of the chick retina. Two papers dealt with retinal disease. In all there were 25 papers in SEM/retinal research before and in 1974. Since 1975 there have been 111 papers which have used SEM …

A Rapid Technique For Counting Cracks In Rocks, W. B. Durham, J. M. Beiriger, H. C. Weed

Scanning Electron Microscopy

Using a scanning electron microscope (SEM) and an image analyzer, we have developed a technique for counting and measuring cracks in rocks which is more efficient than traditional techniques in which an operator performs all image analysis functions. The key aspect of the technique is that black-on-white tracings of fresh cracks, which can be made rather rapidly by an operator, are measured and digitized by an image analyzer. The most time-consuming step in the process has now become the generation of SEM micrographs and pertinent chemical (mineralogical) information, not the quantification of crack structure. The technique has been applied to …

Assessment Of Landsat For Rangeland Mapping, Rush Valley, Utah, United States, National Aeronautics And Space Administration

Range Management

The objective of this investigation is to assess the feasibility of using Landsat MSS (multispectral scanner) data to identify and map cover types for rangeland, and to determine comparative condition of the ecotypes. A supporting objective is to assess the utility of various forms of aerial photography in the process.

If rangeland can be efficiently mapped with Landsat data, as supported by appropriate aerial photography and field data, then uniform standards of cover classification and condition may be applied across the rangelands of the state. Further, a foundation may be established for long-term monitoring of range trend, using the same …

The Economic Impact Of The Apple Maggot And Western Cherry Fruit Fly On Utah's Fruit Industry, Larry K. Bond, T. F. Glover, Clive D. Jorgensen, Anthony H. Hatch

Economic Research Institute Study Papers

Until just recently, the apple maggot (Rhagoletis pomonella) has not been a serious pest in Utah fruit crops. It was first discovered in 1967, but was remote from existing orchards. The first recorded findings in Utah orchards did not occur until 1983 while the Utah Department of Agriculture was conducting survey studies of the western cherry fruit fly (Rhagoletis indifferens).

High Recovery Desalting Of Brackish Water By Electrodialysis: Field Tests At Yuma Desalting Test Facility & At La Verkin Springs, United States Department Of The Interior, Bureau Of Reclamation

Water

An ED (electrodialysis pilot plant achieved high desalting recoveries of nearly 95 percent at the YDTF (Yuma Desalting Test Facility, Arizona) and 92 percent at LVS (La Verkin Springs, Utah) during over 4000 hours of operation. Brine TDS (total dissolved solids) concentrations of nearly 100 000 g/m³ were obtained at LVS, which is a greater brine concentration than is possible using RO (reverse osmosis). The LVS required ED energy (while producing a product TDS concentration of about 1000 g/m³) is estimated to be about one-third the energy requirements of a commercially available distillation brine concentrator. Lowering product …

Utah Science Vol. 45 No. 4, Winter 1984

Utah Science

97 PHOSPHORUS NEEDS OF BEEF CATTLE J. W. Call, J. E. Butcher, J. L. Shupe, A. E. Olson, and J. T. Blake Levels of phosphorus, suggested for normal growth and reproduction, are outlined. Symptoms and lesions of phosphorus deficiency in beef cattle are discussed.

100 FAT CATTLE D. Bailey and B. W. Brorsen The price differences by month and year between Utah and other regional markets for fat cattle were charted and analyzed to help the producer to make effective marketing decisions.

104 THE MALNUTRITION/DIARRHEA CONNECTION R. W. Sidwell, B. B. Barnett, and A. W. Mahoney Using pregnant and newborn …

An Integrated Gis/Remote Sensing Data Base In North Cache Soil Conservation District, Utah: A Pilot Project For The Utah Department Of Agricultures Rims (Resource Inventory And Monitoring System), Douglas J. Wheeler, Merrill K. Ridd, John A. Merola

All U.S. Government Documents (Utah Regional Depository)

The RIMS program is designed "To provide SCD (Soil Conservation District) supervisory with adequate data about agriculture and related natural resources of their district and to use this data for district responsibilities" (Utah Department of Agriculture 1984). The department's guidelines for RIMS go on to describe SCD responsibilities for short and long-term conservation inputs into various state resource plans, giving direction to Agriculture Resource Development Loan fund programs, and giving direction to SCD programs. The guidelines emphasize the established institutional role of the SCD as a local body to deal with local resource issues in the context of a regional …

Surface Roughness Contribution To The Auger Electron Emission, D. Wehbi, C. Roques-Carmes

Scanning Electron Microscopy

Scanning Auger Microscopy (SAM) experiments have shown that z height and θ slope relative to the analysed spot are parameters that contribute to the measured Auger intensity I(z, θ). For greater analysed areas specific to Auger Electron Spectroscopy (AES), the knowledge of height and slope statistical distributions P(z) and P(θ) is required. These functions have been determined by means of profilometric data. The spatial resolution of the used tactile profilometer is similar to that which characterizes AES. A mathematical relationship I { P(z), P(θ) } has been set up for Si samples whose roughness is well defined. On the other …

Twenty-Five Years Of Bonjour, Alfred N. Smith

Faculty Honor Lectures

While considering topics appropriate for this lecture, I became encouraged by the thought that my quarter of a century of experience in foreign language teaching would surely suggest some interesting possibilities. Up until this moment, I had never consciously counted my years of service, and frankly when I realized that I had spent two and one-half decades in front of the blackboard teaching French as a second language mainly to Anglo adults, I began to feel within the rumblings of a need to mark this anniversary in some significant way. Several days later in an amusing conversation with a friend, …

Sputtered Thermal Ion Mass Spectrometry As A New Quantitative Method For In-Depth Analysis, G. Blaise

Scanning Electron Microscopy

The Sputtered Thermal Ion Mass Spectrometry method (STIMS) consists of collecting a part of the matter sputtered from a solid by ion bombardment into a heated cell where it is reduced into atoms. A thermal ionization process or an electron impact process taking place in the cell yields ions which are extracted and mass analyzed. The composition of the solid is determined from ion intensities after calibration of ionization coefficients. It has been demonstrated that the method has an absolute quantitative character. Applications to elemental quantitative analysis (identification of new compounds in diffusion couples, dust particle analysis) and in-depth analysis …

Wave-Length Dispersive Microprobe Analysis Of Coated Samples Of Bulk Tissues, C. Ward Kischer, Thomas M. Teska

Scanning Electron Microscopy

Hypertrophic scars contain highly pleomorphic cells, including many from the erythrocytic series which have been extravasated. The conventional visual mode of SEM cannot distinguish the cell types with certainty except in the case of typical biconcave disc-shaped erythrocytes. Microprobe elemental analysis might be used to differentiate one type from another on the basis of iron and possibly phosphorus (for nucleated cells). Using coated specimens (gold or gold-palladium) precludes simultaneous visual mode SEM with EDX because of energy line interference with phosphorus and other elements. However, wave-length dispersive analysis offers minimal or no interference, and a coated specimen offers the use …

Progress In Element Analysis On A High-Voltage Electron Microscope, W. F. Tivol, D. Barnard, T. Guha

Scanning Electron Microscopy

X-Ray microprobe (XMA) and electron energy-loss (EELS) spectrometers have been installed on the high-voltage electron microscope (HVEM). The probe size has been measured and background reduction is in progress for XMA and EELS as are improvements in electron optics for EELS and sensitivity measurements.

XMA is currently useful for qualitative analysis and has been used by several investigators from our laboratory and outside laboratories. However, EELS background levels are still too high for meaningful results to be obtained. Standards suitable for biological specimens are being measured, and a library for quantitative analysis is being compiled.

Oxidation Of Aluminum Studied By Ion Scattering Spectroscopy (I.S.S) In A Scanning Auger Microscope, M. Gautier, J. P. Duraud, J. P. Vigouroux, C. Le Gressus, R. Shimizu

Scanning Electron Microscopy

The set up of an ion gun, producing a focused beam in the analysis chamber of a Scanning Auger Microscope permits ion scattering experiments: surface studies performed by electron spectroscopies can then be enlarged by Ion Scattering Spectroscopy (I.S.S.) to get additional information.

I.S.S. appears to be very sensitive to the cleanliness of the surface: comparison between Electron Energy Loss Spectroscopy (E.E.L.S.) and I.S.S. studies on clean samples show that I.S.S. can still detect oxygen even when it is not detectable by E.E.L.S.

Preliminary results on oxidation of Al (111) and Al (100) give oxidation curves in good agreement with …

Cross-Sectional Analysis Of Silicon Metal Oxide Semiconductor Devices Using The Scanning Electron Microscope, Daniel S. Koellen, David I. Saxon, Kenneth E. Wendel

Scanning Electron Microscopy

A technique has been developed which enables one to cross-section specific devices or features for examination with the scanning electron microscope (SEM). This method is used for investigation of all facets of microelectronic circuit manufacture from research and development to failure analysis of the finished product.

Selective etching is used to provide contrast to each processed layer. Etch type and sequence, used for delineation, are important to understand since they may add artifacts to the cross-section, leading to erroneous analysis conclusions. The etchant and etch conditions used will be dictated by the information needed from a particular sample.

Etching systems …

Digital Processing Of Electron Energy Loss Spectra And Images, R. D. Leapman, K. E. Gorlen, C. R. Swyt

Scanning Electron Microscopy

Processing in electron energy loss spectroscopy involves both data acquisition and analysis. The interface of an analytical electron microscope to a laboratory computer with a satellite microcomputer dedicated to data acquisition results in a system with a high degree of flexibility. In spectrum acquisition, channels may be selected around specific core edges, or dwell times may be varied continuously as a function of energy loss to reduce the dynamic range of the signal. Data transfer to the host computer allows further analysis such as the removal of plural scattering by spectral deconvolution. Elemental maps and line-scans can be recorded with …

Intracellular Localization Of Heavy Metals In Yeast By X-Ray Microanalysis, Ljerka Kunst, Godfried M. Roomans

Scanning Electron Microscopy

The intracellular localization of heavy metals in yeast cells was studied by means of energy-dispersive X-ray microanalysis. The yeast, Saccharomyces cerevisiae, was pretreated with phosphate and then loaded with different metal ions, by suspending the cells in salt solutions (Ni, Zn, Cd, Pb, Al and Cr). For the analysis, the cells were embedded in gelatin, rapidly frozen, and thin cryosections were cut on a dry knife.

A considerable uptake of divalent cations by the yeast cells was found to occur. The cations were bound to the polyphosphate granules localized in or close to the cell vacuoles. Immediately after phosphate …

Alterations Of The Intracellular Water And Ion Concentrations In Brain And Liver Cells During Aging As Revealed By Energy Dispersive X-Ray Microanalysis Of Bulk Specimens, Gy. Lustyik, I. Zs.-Nagy

Scanning Electron Microscopy

Age dependence of the intracellular concentrations of monovalent ions (Na+, K+ and Cl-) was examined in 1, 11 and 25-month-old rat brain and liver cells by using energy dispersive X-ray microanalysis. The in vivo concentrations of Na+, K+ and Cl- ions were calculated from two different measurements: The elemental concentrations were measured in freeze-dried tissue pieces, and the intracellular water content was determined by means of a recently developed X-ray microanalytic method, using frozen-hydrated and fractured bulk specimens as well as subsequent freeze-drying.

All the single monovalent ion concentrations and consequently, also the total monovalent ion content showed statistically significant …

Study By Scanning Electron Microscopy And Electron Spectroscopy Of The Cascade Of Electron Multiplication In An Insulator Submitted To An Electric Field, J. P. Vigouroux, J. P. Duraud, C. Le Gressus, G. Petite, P. Agostini, C. Boiziau

Scanning Electron Microscopy

An original method for revealing the dielectric heterogeneities on an insulating surface has been developed on creation of an electron multiplication cascade inside the insulator placed in an electric field. The steps of the physical process are: (i) excitation of electrons into the conduction band, (ii) electric field acceleration of the conduction electrons, (iii) ionization of the valence levels, (iv) creation of many more new defects in the vicinity of dielectric heterogeneities, (v) charge localization on defects and appearance of a local residual potential. The potential map is observable by scanning electron microscopy after propagation of the ionizing cascade, but …

String Induced Space Compactification, P. G. Freund, P. Oh, James Thomas Wheeler

All Physics Faculty Publications

Motivated by the possibility of a finite theory of gravity provided by superstrings in ten space-time dimensions, we analyze the problem of space compactification in the context of string dynamics. Such an analysis is hampered by conceptual and technical problems, stemming from the existence of the quantum string's own graviton mode on the one hand, and from Witten's observation of anomalies in a not specially chosen curves space-time on the other hand. Still, in the context of a classical local field presentation of string theory à la Nambu and Hosotani, supplemented by gravitational and Kalb-Ramond interactions, we are able to …

High Accuracy And Automatic Measurement Of The Pattern Linewidth On Very Large Scale Integrated Circuits, H. Yamaji, M. Miyoshi, M. Kano, K. Okumura

Scanning Electron Microscopy

High accuracy measurement of pattern linewidth is particularly important in Very Large Scale Integrated Circuits (VLSI) manufacturing.

The measurement of pattern linewidth has been done by optical methods. However, the optical methods have several problems: as the measured value depends on slope angle at pattern edge, thickness and optical property of film and also substrate, there exists a large difference in size (0.3 μm) between the defined edge and the true edge in case of photoresist linewidth measurements. Especially, the optical methods have severe problems to measure bottom of pattern edge and are unsuitable to measure pattern linewidth in VLSI's …

A Wien Filter Energy Loss Spectrometer For The Dedicated Scanning Transmission Electron Microscope, P. E. Batson

Scanning Electron Microscopy

A Wien filter electron spectrometer has been added to a VG Microscopes, Ltd. HB501 STEM to improve the energy resolution and accuracy of energy loss analysis combined with a high spatial resolution. An energy resolution of 130meV is obtained with a 2mR collection angle at the specimen. The 0.28eV wide field emission energy profile therefore dominates the energy resolution for the device. The energy axis is automatically calibrated by the electrostatic method of scanning, yielding an accuracy and stability of 30meV. A preliminary energy resolution of 0.5eV is demonstrated for 20mR full collection angles at the specimen. Results of experiments …

Scanning Electron Microscopy Of The Lateral Ventricle Of The Pigeon Brain, P. Mestres, K. Rascher, J. D. Delius

Scanning Electron Microscopy

Adult pigeons of both sexes were used for this study. Depending upon the distribution of various surface profiles, for example cilia, microvilli and blebs, ependymal areas with differing surface patterns were distinguished in the lateral ventricle. The topographical locations of these areas with respect to the underlying forebrain nuclei were determined in accord with the atlas of Karten and Hodos (1967). The medial surface (A) of the ventricle was much more densely ciliated than the lateral surface (B). There did not appear to be any correlation between a given surface pattern and a specific type of underlying nervous tissue. Comparison …

Characterization Of Carbonaceous Materials By Correlated Electron And Optical Microscopy And Raman Microspectroscopy, C. Beny-Bassez, J. N. Rouzaud

Scanning Electron Microscopy

Carbonaceous materials differ according to their chemical composition (types of heteroatoms), their structure and their microtexture. So, it is interesting to find methods for characterizing them. We choose to correlate data from Raman microspectroscopy, optical microscopy and transmission electron microscopy. First, we use both graphitizable or non-graphitizable reference carbon series of simple chemical composition in order to follow the structural transformation of the carbonaceous materials according to the evolution of these data throughout heat-treatment of these samples. Then, the coals of different ranks are studied.

The Raman results are correlated with those from electron microscopy, particularly by plotting, for the …

Advances In Non-Contact Thermal-Wave Imaging With Infrared Detection, F. H. Dacol, H. Ermert, R. L. Melcher

Scanning Electron Microscopy

We are making further advances in non-destructive and non-contact thermal imaging with infrared detection. We employ a chopped and scanned electron beam as heat source, a cooled HgCdTe infrared detector as temperature sensor, and digital processing of the measured temperature pattern for display and storage. The results give a convincing, high contrast image of subsurface structures.

The Spectral Momentum Density Of Amorphous Carbon From (E, 2e) Spectroscopy, A. L. Ritter, John R. Dennison, R. Jones

All Physics Faculty Publications

The spectral momentum density of the valence band of an amorphous carbon film has been measured by (e, 2e) spectroscopy. Two "bands," energy as a function of momentum, are resolved. One extends from 23 eV below the Fermi energy to about 10 eV below E_F. The other, ∼ 9 eV below E_F, does not disperse significantly. Although the existence of diamond bonding in the film cannot be ruled out, the bands are more suggestive of the graphite band structure.

Scanning Electron Microscopy Methodology For Study Of The Pathophysiology Of Calcification In Bioprosthetic Heart Valves, Alan C. Nelson, Frederick J. Schoen, Robert J. Levy

Scanning Electron Microscopy

Scanning electron microscope (SEM) morphologic analysis combined with energy dispersive characteristic X-ray (EDX) microprobe analysis provides insight into the mechanisms associated with disease-related crystal formation in biological materials. SEM and EDX were employed in analyzing specimens which were embedded in standard fashion in glycolmethacrylate (JB-4). The specimen surfaces under electron microscope investigation resulted from microtomy used in the preparation of reference light microscope histological sections; thus histology served as a direct reference for the SEM and EDX analyses.

The particular application of these methods was in the study of bioprosthetic heart valve calcification, largely responsible for clinical failure of these …

Detection System For Scanning Electron Microscope, J. Hejna, Z. Radzimski, A. Buczkowski

Scanning Electron Microscopy

The kind of information and the quality of the backscattered electron (BSE) images depend upon numerous features of the detector. Therefore, various types of detectors should be used simultaneously to obtain as much of information as possible. The detection system presented here contains a large area semiconductor detector and a BSE to secondary electrons (SE) converter system. These two different kinds of detectors give different BSE images. After subtracting the signal of a semiconductor detector from that of a converter system, an image with good topography and reduced material contrast can be achieved.

Ultrasonic Microdissection Of Rat Cerebellum For Scanning Electron Microscopy, C. E. Arnett Iii, F. N. Low

Scanning Electron Microscopy

The cerebelli of rats were initially fixed with aldehydes (modified Karnovsky's fixative; 503 mOsM/L) by cardiac perfusion. Blocks of tissue were razor-cut, usually longitudinal to folia, and immersed in the same fluid for 2-4 hours. Three separate methods of treatment followed: (1) immersion in 1% aqueous boric acid, or (2) in 2% phosphate buffered OsO₄ followed by boric acid or (3) in an 8/2 mixture of boric acid and OsO₄. After 18-48 hours immersion the blocks were dehydrated in ascending grades of acetone. They were then exposed to ultrasound in 100% acetone at frequencies of 80 kHz …

Use Of Scanning Electron Microscopy To Study Structural-Functional Relationships In Normal And Diseased Platelets, J. C. Mattson

Scanning Electron Microscopy

This paper reviews the contribution of scanning electron microscopy (SEM) to our understanding of platelet physiology and pathology. Observations of platelet shape changes which accompany activation and the ability to visualize and analyze platelet aggregation and adhesion in three dimensions make this experimental medium an important tool in the evaluation of healthy and diseased platelets. While SEM adds a valuable third dimension to the study of morphology and ultrastructure, its greatest contribution is realized when studies are correlated directly with light and/or transmission electron microscopic observations and with studies of functional capacity.