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Enhanced Biofilm Formation Between R. Insidiosa And L. Monocytogenes, Sophie G. Unger, Matthew J. Wargo Jan 2023

Enhanced Biofilm Formation Between R. Insidiosa And L. Monocytogenes, Sophie G. Unger, Matthew J. Wargo

UVM Patrick Leahy Honors College Senior Theses

Listeria monocytogenes is a bacterial species that can contaminate a wide range of food products and cause foodborne illness. Ingestion of L. monocytogenes-contaminated food can be especially dangerous for individuals who are immunocompromised, pregnant, or over the age of 65. Previous research showed that a single strain of L. monocytogenes may form enhanced biofilms with a single strain of Ralstonia insidiosa, a bacterium commonly found in potable water systems. This study investigated how common the L. monocytogenes and R. insidiosa biofilm enhancement is across strains of each species and other species in the genus Ralstonia. Our results …


An Environmental Approach To Food Safety Assessment Using Artisan Cheese And Fresh Produce As Model Systems, Marie Limoges Jan 2019

An Environmental Approach To Food Safety Assessment Using Artisan Cheese And Fresh Produce As Model Systems, Marie Limoges

Graduate College Dissertations and Theses

This dissertation examined recently issued regulatory standards using cheese and produce as model systems. FDA's 2015 Domestic and Imported Cheese and Cheese Products Compliance Program Guidelines (CPG) E. coli standards on cheese safety, and the extent to which these standards affect domestic and imported cheese commerce, was assessed. Results from FDA's Domestic and Imported Cheese Compliance Program for samples collected between January 1, 2004 and December 31, 2006 were analyzed. Of 3,007 cheese samples tested for non-toxigenic E. coli, 76% (2,300) of samples exceeded 10/g, FDA's target for regulatory activity. In cheese samples containing E. coli levels of 10/g and …


Ecological Dynamics In Compost-Amended Soils And The Resulting Effects On Escherichia Coli Survival, Anya Cutler Jan 2016

Ecological Dynamics In Compost-Amended Soils And The Resulting Effects On Escherichia Coli Survival, Anya Cutler

Graduate College Dissertations and Theses

Escherichia coli (E. coli) are common and typically innocuous copiotrophic bacteria found in the mammalian gut microbiome. However, over the past 30 years, pathogenic E. coli have been responsible for several outbreaks of foodborne illness linked to contaminated produce. The introduction of Escherichia coli to an agricultural soil, via contaminated water, compost, or raw manure, exposes the bacterium to a medley of ecological forces not found in a mammalian gut environment. This study assesses a variety of abiotic and biotic soil factors that influence the ability of an "invasive" copiotrophic coliform bacterium to survive in compost-amended agricultural soil. The study …


Food Safety Education And Disparities In North Carolina Emergency Food, Ashley Chaifetz, Benjamin Chapman Jun 2015

Food Safety Education And Disparities In North Carolina Emergency Food, Ashley Chaifetz, Benjamin Chapman

Food Systems Summit 2015

Each year, an estimated 48 million Americans (1 in 6) contract foodborne illness (Scallan, 2011) stemming from grocery stores, hospitals, day care centers, church banquets, county fairs, restaurants, private homes, schools, and even food banks (CDC FOOD, 2011). However, little research has explored the potential connection between food-insecure populations and risk of foodborne illness, especially for emergency foods (c.f. Henley et al., 2012; Koro et al., 2010; Quinlan, 2013). For the majority of people who seek food assistance, food pantries are a fixed part of food sources; that is, the pantries are for subsistence and no longer just “emergency food” …