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Angiotensinogen Gene Silencing Reduces Lipid Accumulation And Inflammation In Cultured 3t3-L1 Adipocytes, Wenting Xin Carroll

Masters Theses

Obesity is characterized by metabolic complications which are related to several life-threatening diseases. Dysregulated inflammatory adipokines secretion from adipose tissue is believed as the major contributor to obesity-associated local and systemic inflammation, insulin resistance, and other metabolic dysfunctions.

Numerous studies in our lab and others pointed to the role of local adipose tissue renin-angiotensin system (RAS) in the pathogenesis of obesity, inflammation and insulin resistance. We hypothesized that adipocytes-derived angiotensinogen (Agt) played a critical role in adipogenesis and/or lipogenesis as well as adipose inflammation. To test, we established 3T3-L1 preadipocytes stably transfected with Agt-shRNA or scrambled sequence (Sc-shRNA). Transfected preadipocytes …

Kids Can Bike! A Parks And Recreation Program To Promote Youth Cycling, Jessica Lynn Chandler

Masters Theses

Physical inactivity is a major concern, and is believed to contribute to the high prevalence of childhood obesity in U.S. schoolchildren. To address this problem, the Knoxville Area Coalition on Childhood Obesity launched a ‘Kids Can BIKE!’ program in 2012. The bicycling program is seven weeks long and is designed to increase physical activity, teach safe cycling skills, and explore Knoxville parks and greenways while having fun. The program targeted children in grades 4 and 5 who were already enrolled in an afterschool or summer program. Once a week, the children were transported to a location where they could safely …