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University of South Florida

2007

Marine

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Molecular And Genomic Studies Of Temperate Phages From Halomonas Aquamarina And Bacillus Spp. Isolates From The Gulf Of Mexico, Jennifer M. Mobberley Jun 2007

Molecular And Genomic Studies Of Temperate Phages From Halomonas Aquamarina And Bacillus Spp. Isolates From The Gulf Of Mexico, Jennifer M. Mobberley

USF Tampa Graduate Theses and Dissertations

Viruses are the most abundant biological entities in the ocean and are believed to contribute to nutrient cycling, bacterial diversity, and horizontal gene exchange. However, little is known about the relationship between temperate phages and their hosts in marine environments. In this thesis, phage-host systems from the Gulf of Mexico were used to study the influence of temperate phages in bacteria. PhiHAP-1 is a temperate myovirus induced with mitomycin C from Halomonas aquamarina isolate. The genome of this phage was 39,245 nucleotides long and contained 46 predicted genes. Besides genes involved in lysogeny, PhiHAP-1 contained a protelomerase, which is responsible …