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Articles 1 - 7 of 7
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The Distribution Of Fitness Effects Of Spontaneous Mutations In Vibrio Fischeri, Chelsea K. Jones
The Distribution Of Fitness Effects Of Spontaneous Mutations In Vibrio Fischeri, Chelsea K. Jones
Honors Theses and Capstones
Mutations are the ultimate source of the biological diversity on which natural selection acts, but the vast majority of these mutations are harmful. As such, mutations lead to disease states like cancer, extinction of small populations, and can drive pathogen evolution. Unfortunately, because mutations are rare and past studies have been subject to detection biases, very little is known about the distribution of fitness effects from naturally occurring mutations. In this study, we used mutation accumulation and full genome sequencing to capture naturally occurring mutations before they were exposed to the sieve of natural selection in Vibrio fischeri. We …
Insights From Project Feederwatch: Changes In The Abundance And Occurrence Of Birds In New Hampshire Over The Past 24 Years, Lee A. Sutcliffe
Insights From Project Feederwatch: Changes In The Abundance And Occurrence Of Birds In New Hampshire Over The Past 24 Years, Lee A. Sutcliffe
Honors Theses and Capstones
Changes in the climate and land use over time can lead to changes in the composition of wildlife communities. Using data from Project FeederWatch, we examine trends in the abundance and occurrence of birds documented in the winters from 1988 to 2012 in New Hampshire. Changes in abundance and occurrence are summarized for individual bird species as well as across species based on life history traits. In addition, we examined trends for the state as a whole as well as in subregions. We discuss these changes with regards to the variations of climate and land use that are occurring throughout …
Diel Vertical Migration Strategies Of Zooplankton In Oligotrophic Russell Pond, New Hampshire, Kelsey Ann Dobe
Diel Vertical Migration Strategies Of Zooplankton In Oligotrophic Russell Pond, New Hampshire, Kelsey Ann Dobe
Honors Theses and Capstones
Russell Pond is an ultra-oligotrophic lake with low chlorophyll a (1.9 mg L-1), total phosphorus (3.4 mg L-1), high Secchi Disk (10.4 m) and high light transmission (water coefficient of water, kw=0.33). Vertical migration of Chaoborus, Bosmina, Daphnia, and copepods were examined using net collections of zooplankton discrete depth counts and sonar. Three contrasting patterns of vertical migration were observed in Russell Pond. Chaoborus punctipennis larvae vertical migration began at 7 pm and migrated through the entire lake water column, a total of 23 m from the sediments to the surface water in …
Kingswood Lake Management Plan, Sarah Martina Tierney
Kingswood Lake Management Plan, Sarah Martina Tierney
Honors Theses and Capstones
High water quality and natural buffers exist on Kingswood Lake located in Brookfield, New Hampshire. A comprehensive lakes inventory (CSI) was recorded and health of the land, wildlife, and waterways were observed. Educational outreach was taken to inform the local community and to instruct them on proper stewardship of the land. A completed watershed management plan was constructed including key recommendation of limiting nitrogen loading in the area, checking septic systems for leaching, stabilizing shoreline from erosion, and establishing man-made rain gardens to help preserve water quality. The Kingswood watershed management plan acts as a guide for the town of …
Investigating The Interactions Between Cyanobacteria And Vibrio Parahaemolyticus, Caroline E. Ward
Investigating The Interactions Between Cyanobacteria And Vibrio Parahaemolyticus, Caroline E. Ward
Honors Theses and Capstones
One well-known pathogen that has been the topic of many recent studies is Vibrio parahaemolyticus, which causes thousands of foodborne illnesses a year, mostly from the ingestion of raw or undercooked oysters. It has been shown cyanobacteria can act as a long-term reservoir of Vibrio cholerae, another pathogenic Vibrio, by encasing the cells within mucilaginous sheaths during which Vibrios enter a viable but non-culturable state. In this study we investigated the interaction of V. parahaemolyticus with cyanobacteria to determine whether cyanobacteria aid in the longevity and survival of V. parahaemolyticus. We found that non-pathogenic V. parahaemolyticus …
The Pleiotropic Effects Of Beneficial Mutations Of Adapted Escherichia Coli Populations, Brian Scott Van Dam
The Pleiotropic Effects Of Beneficial Mutations Of Adapted Escherichia Coli Populations, Brian Scott Van Dam
Honors Theses and Capstones
Mutations that improve fitness in one environment can often be beneficial, deleterious, or neutral in alternative environments. When a single mutation effects fitness in multiple environments, it is said to be a pleiotropic, which can have important consequences for niche specialization, niche expansion, speciation, and even extinction in the face of environmental change. While previous studies have revealed that pleiotropy is nearly universal, the role of adaptive history in the spectrum of pleiotropic effects has yet to undergo detailed experimental observation. Using experimental evolution we gathered beneficial mutations in a previously adapted strain of Escherichia coli growing in the same …
Effects Of Keratin Filaments On Erk Signaling During Fas-Induced Death Of Cervical Cancer (Hela) Cells, Amanda Berger
Effects Of Keratin Filaments On Erk Signaling During Fas-Induced Death Of Cervical Cancer (Hela) Cells, Amanda Berger
Honors Theses and Capstones
Survival of cancer cells is influenced by a variety of factors, including physical elements such as keratin filaments. We know HeLa cells containing or lacking keratin 8/18 intermediate filaments (K+ and K- cells, respectively) are more sensitive to the death-inducing effects of Fas agonist compared to the cytokines tumor necrosis factor alpha (TNF-α) or TNF-related apoptosis-inducing ligand. Additionally, K- cells are more sensitive to Fas-induced death than K+ as shown by previous studies using mitochondrial activity and caspase activation assays. In the current study we tested the hypothesis that keratin filaments associate with the mitogen activated protein kinase (MAPK) cascade …