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Transmittance And Reflectance Measurements Of Corn Leaves From Plants With Different Nitrogen And Water Supply, James S. Schepers, T. M. Blackmer, Wallace Wilhelm, M. Resende Nov 1996

Transmittance And Reflectance Measurements Of Corn Leaves From Plants With Different Nitrogen And Water Supply, James S. Schepers, T. M. Blackmer, Wallace Wilhelm, M. Resende

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrogen is essential for crop production, but also contributes to eutrophication of surface water and degradation of drinking water quality. Modern corn production requires relatively large quantities of N, which are generally supplied by fertilizers. Over-application of N fertilizers and animal wastes frequently results in nitrate leaching. Synchronizing N availability with crop N need offers the potential to protect the environment without sacrificing production. Tools are needed to rapidly and easily monitor crop N status to make timely decisions regarding fertilizer application. Analytical and optical techniques were evaluated with greenhouse grown corn at silking to evaluate several methods to monitor …


Monoclonal Antibodies For Detection Of The H7 Antigen Of Escherichia Coli, Yongsheng He, James E. Keen, Ralph B. Westerman, E. Travis Littledike, Jimmy Kwang Sep 1996

Monoclonal Antibodies For Detection Of The H7 Antigen Of Escherichia Coli, Yongsheng He, James E. Keen, Ralph B. Westerman, E. Travis Littledike, Jimmy Kwang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two murine monoclonal antibodies (MAbs) (2B7 and 46E9-9) reactive with the H7 flagellar antigen of Escherichia coli were produced and characterized. A total of 217 E. coli strains (48 O157:H7, 4 O157:NM, 23 O157:non-H7, 22 H7:non-O157, and 120 non-O157:non-H7), 17 Salmonella serovars, and 29 other gramnegative bacteria were used to evaluate the reactivities of the two MAbs by indirect enzyme-linked immunosorbent assay (ELISA). Both MAbs reacted strongly with all E. coli strains possessing the H7 antigen and with H23- and H24-positive E. coli strains. Indirect ELISA MAb specificity was confirmed by inhibition ELISA and by Western blotting (immunoblotting), using partially …


Spikelet And Floret Naming Scheme For Grasses With A Spike Inflorescence, Wallace Wilhelm, Gregory S. Mcmaster Jul 1996

Spikelet And Floret Naming Scheme For Grasses With A Spike Inflorescence, Wallace Wilhelm, Gregory S. Mcmaster

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Effective schemes to name uniquely and define parts of grass plants allow researchers to communicate results of their experiments accurately and precisely. Naming schemes for vegetative parts of grass plants have been developed; however, no system exists to name uniquely parts of the inflorescence. In this report, we present a method to extend existing systems to name parts of grass spikes. Spikelet positions are denoted by the letter S and numbered acropetally along the rachis. For example, the third spikelet position from the base of the rachis is named S3. Likewise, floret positions along the rachilla are named F (or …


The Structural Gene Encoding Human Enterotoxigenic Escherichia Coli Pcfo20 Is Homologous To That For Porcine 987p, Gloria Inés Viboud, Gunhild Jonson, Evelyn A. Dean-Nystrom, Ann-Mari Svennerholm Apr 1996

The Structural Gene Encoding Human Enterotoxigenic Escherichia Coli Pcfo20 Is Homologous To That For Porcine 987p, Gloria Inés Viboud, Gunhild Jonson, Evelyn A. Dean-Nystrom, Ann-Mari Svennerholm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Putative colonization factor PCFO20 was recently identified in an enterotoxigenic Escherichia coli (ETEC) strain of serogroup O20 isolated from a child with diarrhea in Argentina. The gene encoding the structural subunit of PCFO20 fimbriae, fotA, was cloned from strain ARG-2 in the expression phage vector lambda ZAP Express. One positive clone, pGV29, that carried a 3.3-kb fragment was identified on the basis of fimbrillin production by using a monospecific rabbit anti-PCFO20 serum. Nucleotide sequencing of a 1.3-kb Sau3A-ClaI fragment of the subclone pGV292 containing the region coding for PCFO20 fimbrillin revealed two open reading frames of …


Planting Date And Residue Rate Effects On Growth, Partitioning, And Yield Of Corn, Stephan Swanson, Wallace Wilhelm Mar 1996

Planting Date And Residue Rate Effects On Growth, Partitioning, And Yield Of Corn, Stephan Swanson, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Corn (Zea mays L.) growth has been shown to be affected by planting date and amount of residue on the soil surface. The objective of this study was to determine if the adverse effects of cool soil temperatures on early seedling growth often associated with surface residues can be overcome by planting at later dates. Corn was planted at several planting dates (late April through early June) into soil that had residue applied at rates of 0, 33, 66, and 100% of that produced by the previous crop. In general, spring-applied residue rates had little effect on measured plant …


Tallgrass Prairie Vegetation Response To Spring Burning Dates, Fertilizer, And Atrazine, Robert B. Mitchell, Robert A. Masters, Steven S. Waller, Kenneth Moore, Linda J. Young Mar 1996

Tallgrass Prairie Vegetation Response To Spring Burning Dates, Fertilizer, And Atrazine, Robert B. Mitchell, Robert A. Masters, Steven S. Waller, Kenneth Moore, Linda J. Young

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Tallgrass prairies provide an important source of hay and summer forage in eastern Nebraska. A study was conducted in 1989 and 1990 on 2 late seral tallgrass prairies near Lincoln and Virginia, Nebraska to determine if production of selected components of tallgrass prairie communities could be altered by burning (not burned, or burned in either early, mid-, or late spring) and applying fertilizer (0 and 67-23 kg N-P ha-1) and atrazine [6- chloro-N-ethyl-N'-(1-methylethyl)-1,3,5-triazine-2,4-diamine] (0 and 2.2 kg a.i. ha-1). Vegetation was harvested the year treatments were applied at about 30-day intervals starting in June and ending …


Harvest Frequency And Burning Effects On Monocultures Of 3 Warm-Season Grasses, Gregory J. Cuomo, Bruce Anderson, Linda J. Young, Wally Wilhelm Mar 1996

Harvest Frequency And Burning Effects On Monocultures Of 3 Warm-Season Grasses, Gregory J. Cuomo, Bruce Anderson, Linda J. Young, Wally Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Harvest frequency and burning can affect forage yield of monocultures of switchgrass (Panicurn virgutum L.), big bluestem (Andropogon gerurdii Vitman), and Indian grass [Sorghastrum nutuns (L.) Nash]. Current information is based largely on results from mixed stands. A field experiment was established in 1986, and from 1988 to 1991 treatments were applied with burning in March, April, or May plus an unburned control. Growing season yield was measured by harvesting 1 (June), 2 (June and July), or 3 (June, July, and August) times with unharvested control plots included. End-of-season standing crop from all plots was determined …


Immunological Responses In The Mouse Host To A Cloned Antigen Of Taeniacrassiceps, Dolores E. Hill, Dante S. Zarlenga Jan 1996

Immunological Responses In The Mouse Host To A Cloned Antigen Of Taeniacrassiceps, Dolores E. Hill, Dante S. Zarlenga

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Adult female Swiss-Webster mice were immunized either intraperitoneally (IP) or subcutaneously (SQ) with cyst fluid or a genetically engineered fusion protein, Taenia carassiceps antigen 2-maltose binding protein (TCA2-MBP) from Taenia crassiceps metacestodes, or with live, non-budding cysts SQ, and then challenged IP with T. crassiceps metacestodes and necropsied 9 weeks later. Numbers of peripheral blood eosinophils were increased after IP immunization, but were not increased after SQ immunization or with SQ cysts given before the challenge infection. Eosinophil numbers gradually decreased over the course of the experiment, and were not found in increased numbers in the blood or peritoneal cavity …


Semiochemistry Of Cabbage Bugs (Heteroptera: Pentatomidae: Eurydema And Murgada, Jeffrey R. Aldrich, James W. Avery, Chang-Joo Lee, Jennifer C. Graf, Dawn J. Harrison, Ferdinando Bin Jan 1996

Semiochemistry Of Cabbage Bugs (Heteroptera: Pentatomidae: Eurydema And Murgada, Jeffrey R. Aldrich, James W. Avery, Chang-Joo Lee, Jennifer C. Graf, Dawn J. Harrison, Ferdinando Bin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The semiochemistry of the common North American pest of crucifers, Murgantia histrionica (Hahn) (the harlequin bug), and two related European species, Eurydema ventrale L. and E. oleraceum L., was investigated.


Imidazolinone Herbicides Improve Restoration Of Great Plains Grasslands, Robert A. Masters, Scott Nissen, Roch E. Gaussoin, Daniel Beran, Robert Stougaard Jan 1996

Imidazolinone Herbicides Improve Restoration Of Great Plains Grasslands, Robert A. Masters, Scott Nissen, Roch E. Gaussoin, Daniel Beran, Robert Stougaard

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The productivity and native species diversity of Great Plains grasslands have been substantially reduced by past management that facilitated the establishment of invasive exotic weeds and displacement of native species. Management strategies are needed to rapidly restore the productive capacity and biological diversity of these degraded grasslands. Critically important phases of the grassland restoration process are the reintroduction and establishment of native species. Weed interference is the primary constraint to successful establishment of native plants. The goal of our research is to develop strategies that use multiple technologies, including herbicides, to expedite grassland revegetation with native grasses and forbs. Imidazolinon …


Leafy Spurge (Euphorbia Esula) Genotype Affects Gall Midge (Spurgia Esulae) Establishment, Rodney G. Lym, Scott Nissen, Martha Rowe, Donald J. Lee, Robert A. Masters Jan 1996

Leafy Spurge (Euphorbia Esula) Genotype Affects Gall Midge (Spurgia Esulae) Establishment, Rodney G. Lym, Scott Nissen, Martha Rowe, Donald J. Lee, Robert A. Masters

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Greenhouse cage studies were conducted to determine the influence of shoot morphology and genetic variation on establishment of Spurgia sulae gall midge on seven leafy spurge genotypes. The genotypes were collected from South Dakota, North Dakota, Nebraska, Montana, Wyoming, Manitoba, and Austria. Genotypes from South Dakota and Nebraska were most susceptible to gall formation and had the highest larvae survival, while the genotypes from Montana and Manitoba were most resistant. Morphological characteristics of the leafy spurge stem tips, such as stem diameter leaf, length, width, and area did not correlate with gall formation or larvae survival. Chloroplast DNA restriction fragment …


Internal Drainage Through Fine-Textured Subsoils At Two Sites In North Dakota, Todd P. Trooien, Brian J. Wienhold, George A. Reichman Jan 1996

Internal Drainage Through Fine-Textured Subsoils At Two Sites In North Dakota, Todd P. Trooien, Brian J. Wienhold, George A. Reichman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

To determine if the internal drainage (downward movement of water out of the root zone) was adequate, we measured the movement of water out of the root zone in bordered plots planted to alfalfa. We applied three water quantity treatments: irrigation plus precipitation equal to one, two, and three times the calculated evapotranspiration (1ET, 2ET, and 3ET); and two irrigation water quality treatments: electrical conductivity (ECiw) of 0.1 S/m, and sodium adsorption ratio (SARiw) of 4 and ECiw = 0.34 S/m, SARiw = 16. Each treatment was replicated three times. Internal drainage amounts during …


Culicoides Variipennis And Bluetongue-Virus Epidemiology In The United States1, Walter J. Tabachnick Jan 1996

Culicoides Variipennis And Bluetongue-Virus Epidemiology In The United States1, Walter J. Tabachnick

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The bluetongue viruses are transmitted to ruminants in North America by Culicoides vuriipennis.US annual losses of approximately $125 million are due to restrictions on the movement of livestock and germplasm to bluetongue-free countries. Bluetongue is the most economically important arthropod-borne animal disease in the United States. Bluetongue is absent in the northeastern United States because of the inefficient vector ability there of C. variipennis for bluetongue. The vector of bluetongue virus elsewhere in the United States is C. vuriipennis sonorensis. The three C. variipennis subspecies differ in vector competence for bluetongue virus in the laboratory. Understanding C.vuriipennis genetic variation controlling …


Chloroplast Dna And Nuclear Dna Content Variations Among Cultivars Of Switchgrass, Panicum Virgatum L., Sherry J. Hultquist, Kenneth P. Vogel, D. J. Lee, K. Arumuganathan, S. Kaeppler Jan 1996

Chloroplast Dna And Nuclear Dna Content Variations Among Cultivars Of Switchgrass, Panicum Virgatum L., Sherry J. Hultquist, Kenneth P. Vogel, D. J. Lee, K. Arumuganathan, S. Kaeppler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass, Panicum virgatum L., is a native, cross-pollinated, morphologically diverse species with an array of ploidy levels and ecotypes. Switchgrass is found throughout most of the USA and Canada, primarily east of the Rocky Mountains and south of Hudson Bay. The objective of this research was to determine if chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) occur among switchgrass cultivars and experimental strains that differ in ploidy level or ecotype classification. Eighteen switchgrass cultivars or experimental strains representative of reported ecotypes, ploidy levels, and the geographical range of switchgrass were surveyed for cpDNA polymorphisms by means of four restriction …


Energy Production From Forages (Or American Agriculture-Back To The Future), Kenneth P. Vogel Jan 1996

Energy Production From Forages (Or American Agriculture-Back To The Future), Kenneth P. Vogel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

At the turn of the century, with the exception of trains and water transportation, the transportation and agriculture industries of the U.S. were powered largely by herbaceous biomass. The herbaceous biomass was converted to usable energy by draft animals, primarily horses and mules. After 1900, automobiles, trucks, and tractors began to be used in transportation and agriculture. However, in 1920 there were still 25 million horses and mules on farms and ranches and 2 million draft animals in the cities of the United States (Ensminger 1955; (census of Agriculture 1920). The energy requirements of these animals were considerable. In the …


Permethrin-Impregnated Yarn: Longevity Of Efficacy And Potential Use On Cylindrical Fiberglass Stable Fly (Diptera: Muscidae) Traps, Jerome Hogsette, Joseph Ruff Jan 1996

Permethrin-Impregnated Yarn: Longevity Of Efficacy And Potential Use On Cylindrical Fiberglass Stable Fly (Diptera: Muscidae) Traps, Jerome Hogsette, Joseph Ruff

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Efficacy of permethrin-impregnated orlon yam against stable flies, Stomoxys calcitrans (L.), was 6-8 wk under simulated field conditions. Numbers of stable flies captured on corrugated cylindrical fiberglass traps painted with an adhesive indicated that these traps are attractive enough to be used in the field with treated yam applied. Reasons for variability of yam impregnation and the use of yam on cylinder traps are discussed.


Registration Of 'Shawnee' Switchgrass, Kenneth P. Vogel, A. A. Hopkins, K. J. Moore, K. D. Johnson, I. T. Carlson Jan 1996

Registration Of 'Shawnee' Switchgrass, Kenneth P. Vogel, A. A. Hopkins, K. J. Moore, K. D. Johnson, I. T. Carlson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

'Shawnee' switchgrass (Panicum virgatum L.) (Reg. no. CV-181, P1 591824) was released on 13 July 1995 by the USDA-ARS, the Agricultural Research Division of the University of Nebraska- Lincoln, the USDA-NRCS, the Iowa Agriculture Experiment Station, and the Purdue Agricultural Research Program. The cultivar was developed in cooperative research by the USDA-ARS and the University of Nebraska at the University of Nebraska Agricultural Research and Development Center at Mead, using plant germplasm from the Elsberry Plant Materials Center, USDA-NRCS. Shawnee was evaluated in cooperative trials with the USDA-ARS, the University of Nebraska, Iowa State University, and Purdue University.


Carbonate And Gypsum, Richard H. Loeppert, Donald L. Suarez Jan 1996

Carbonate And Gypsum, Richard H. Loeppert, Donald L. Suarez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The carbonate minerals and gypsum exert a dominating influence on soils in
which they are present because of their relatively high solubility, and in the case
of the carbonates, their alkalinity and pH buffering properties. In this chapter,
procedures for the determination of quantity, reactivity and equilibrium relations
of these minerals will be discussed.


Registration Of 'Pronghorn' Prairie Sandreed Grass, Kenneth P. Vogel, L. C. Newell, Erling T. Jacobson, John E. Watkins, Patrick E. Reece, Dennis E. Bauer Jan 1996

Registration Of 'Pronghorn' Prairie Sandreed Grass, Kenneth P. Vogel, L. C. Newell, Erling T. Jacobson, John E. Watkins, Patrick E. Reece, Dennis E. Bauer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

'Pronghorn' prairie sandreed grass [Calamovilfa longifolia (Hook.) Scribn.] (Reg. no. CV-182, P1592392) was released by the USDAARS, the Agricultural Research Division of the University of Nebraska-Lincoln, and the USDA-SCS on 5 May 1988. The cultivar was developed in cooperative research by the USDA-ARS and the University of Nebraska using plant germplasm from this cooperative program and from the Manhattan Plant Materials Center, USDA-NRCS, Manhattan, KS.


Bromegrasses, Kenneth P. Vogel, K. J. Moore, Lowell E. Moser Jan 1996

Bromegrasses, Kenneth P. Vogel, K. J. Moore, Lowell E. Moser

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The bromegrasses belong to the genus Bromus of which there are some 100 species (Gould & Shaw, 1983). The genus includes both annual and perennial cool season species adapted to temperate climates. Hitchcock (1971) described 42 bromegrass species found in the USA and Canada of which 22 were native (Gould & Shaw, 1983). Bromus is the Greek word for oat and refers to the panicle inflorescence characteristic of the genus. The bromegrasses are C3 species (Krenzer et aI., 1975; Waller & Lewis, 1979).


Development Of House Flies (Diptera: Muscidae) In Sand Containing Varying Amounts Of Manure Solids And Moisture, Jerome Hogsette Jan 1996

Development Of House Flies (Diptera: Muscidae) In Sand Containing Varying Amounts Of Manure Solids And Moisture, Jerome Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

House flies, Musca domestica L., developed in 200 cm3 of coarse sand containing just 1 ml (0.47%) of dairy manure solids and 10 ml (4.74%) of moisture. At these levels, development was slow (21.5 d from 1st instar to adult), adult survival was low (7.5%), but successful development did occur. At higher manure and moisture levels, rates of development and survival were similar to those reported previously. All soil samples collected from a feedlot dairy contained higher levels of manure solids than the highest level tested in the laboratory. The implications for fly control in soil/manure mixtures and the …