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University of Nebraska - Lincoln

School of Biological Sciences: Faculty Publications

Series

2003

Aptostichus

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An Analysis Of The Secondary Structure Of The Mitochondrial Large Subunit Rrna Gene (16s) In Spiders And Its Implications For Phylogenetic Reconstruction, Stacey Dewitt Smith, Jason E. Bond Jan 2003

An Analysis Of The Secondary Structure Of The Mitochondrial Large Subunit Rrna Gene (16s) In Spiders And Its Implications For Phylogenetic Reconstruction, Stacey Dewitt Smith, Jason E. Bond

School of Biological Sciences: Faculty Publications

We investigated the pattern of molecular variation with respect to secondary structure in the 16S ribosomal RNA gene and its phylogenetic implications for arachnids with a focus on spiders. Based on a model by Gutell et al. (1996), secondary structures were proposed for the 3' half of 16S in the mygalomorph spider Aptostichus atomarius. Models were also constructed for a hypervariable length of the 16S in three other arachnids, which revealed a trend of stem and loop reduction in more advanced arachnids. Using a simple statistical approach to compare functional regions, we found that internal and external loops are …