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University of Nebraska - Lincoln

Department of Biological Systems Engineering: Papers and Publications

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Optimized Gadolinium-Do3a Loading In Raftpolymerized Copolymers For Superior Mr Imaging Of Aging Blood–Brain Barrier, Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest Kievit, Anthony J. Covertine Jul 2024

Optimized Gadolinium-Do3a Loading In Raftpolymerized Copolymers For Superior Mr Imaging Of Aging Blood–Brain Barrier, Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest Kievit, Anthony J. Covertine

Department of Biological Systems Engineering: Papers and Publications

The development of gadolinium-based contrast agents (GBCAs) has been pivotal in advancing magnetic resonance imaging (MRI), offering enhanced soft tissue contrast without ionizing radiation exposure. Despite their widespread clinical use, the need for improved GBCAs has led to innovations in ligand chemistry and polymer science. We report a novel approach using methacrylate-functionalized DO3A ligands to synthesize a series of copolymers through direct reversible addition-fragmentation chain transfer (RAFT) polymerization. This technique enables precise control over the gadolinium content within the polymers, circumventing the need for subsequent conjugation and purification steps, and facilitates the addition of other components such as targeting ligands. …


Sensitization Of Europium Oxide Nanoparticles Enhances Signal-To- Noise Over Autofluorescence With Time-Gated Luminescence Detection, Hunter Miller, Jessica Q. Wallace, Hui Li, Xing-Zhong Li, Ana De Bettencourt-Dias, Forrest Kievit Jun 2024

Sensitization Of Europium Oxide Nanoparticles Enhances Signal-To- Noise Over Autofluorescence With Time-Gated Luminescence Detection, Hunter Miller, Jessica Q. Wallace, Hui Li, Xing-Zhong Li, Ana De Bettencourt-Dias, Forrest Kievit

Department of Biological Systems Engineering: Papers and Publications

Clinical translation of nanoparticle-based therapeutics has been limited, and a lack of preclinical delivery characterization is partly to blame, limiting our understanding of the mechanisms of failure. The improvement of the preclinical delivery assessment requires nanoparticles with higher detectability. This work focused on the exploration of several aromatic carboxylic ligands (terephthalic acid, quinaldic acid, and kynurenic acid) for the sensitization of europium oxide nanoparticles with a long emission lifetime to overcome cellular autofluorescence, a key confounder of detection in luminescence-based bioimaging. A facile one-pot synthesis and ligand exchange process generated and sensitized ultrasmall Eu2O3 cores. As reflected …


Fabrication Of A Low-Cost Drum Seeder For Paddy, Muhammad Rashed Al Mamun, Md Rejaul Karim, Md Towfiqur Rahman, Md Janibul Alam Soeb, Md Imranul Alam Jun 2024

Fabrication Of A Low-Cost Drum Seeder For Paddy, Muhammad Rashed Al Mamun, Md Rejaul Karim, Md Towfiqur Rahman, Md Janibul Alam Soeb, Md Imranul Alam

Department of Biological Systems Engineering: Papers and Publications

Rice is a staple food crop. Bangladesh produces it extensively which raised the nation into the fourth-largest rice-grower in the world. Transplanting or direct seeding is a major task in rice cultivation where the manual transplanting method requires a huge labor cost which is beyond control in a peak season. Although mechanical rice transplanters become popularization land preparation and seedling rising as a pre transplanting task is costly and labor-intensive. On the other hand, manual broadcasting and machine seeding direct method still exist due to the above method’s problem. Considering spacing accuracies for intercultural operation, drum seeders are an easy …


Identification Of Compounds That Cause Axonal Dieback Without Cytotoxicity In Dorsal Root Ganglia Explants And Intervertebral Disc Cells With Potential To Treat Pain Via Denervation, Fei San Lee, Uyen N. Nguyen, Eliza J. Munns, Rebecca A. Wachs May 2024

Identification Of Compounds That Cause Axonal Dieback Without Cytotoxicity In Dorsal Root Ganglia Explants And Intervertebral Disc Cells With Potential To Treat Pain Via Denervation, Fei San Lee, Uyen N. Nguyen, Eliza J. Munns, Rebecca A. Wachs

Department of Biological Systems Engineering: Papers and Publications

Low back pain, knee osteoarthritis, and cancer patients suffer from chronic pain. Aberrant nerve growth into intervertebral disc, knee, and tumors, are common pathologies that lead to these chronic pain conditions. Axonal dieback induced by capsaicin (Caps) denervation has been FDA-approved to treat painful neuropathies and knee osteoarthritis but with shortterm efficacy and discomfort. Herein, we propose to evaluate pyridoxine (Pyr), vincristine sulfate (Vcr) and ionomycin (Imy) as axonal dieback compounds for denervation with potential to alleviate pain. Previous literature suggests Pyr, Vcr, and Imy can cause undesired axonal degeneration, but no previous work has evaluated axonal dieback and cytotoxicity …


Identification Of Compounds That Cause Axonal Dieback Without Cytotoxicity In Dorsal Root Ganglia Explants And Intervertebral Disc Cells With Potential To Treat Pain Via Denervation, Fei San Lee, Uyen N. Nguyen, Eliza J. Munns, Rebecca A. Wachs May 2024

Identification Of Compounds That Cause Axonal Dieback Without Cytotoxicity In Dorsal Root Ganglia Explants And Intervertebral Disc Cells With Potential To Treat Pain Via Denervation, Fei San Lee, Uyen N. Nguyen, Eliza J. Munns, Rebecca A. Wachs

Department of Biological Systems Engineering: Papers and Publications

Low back pain, knee osteoarthritis, and cancer patients suffer from chronic pain. Aberrant nerve growth into intervertebral disc, knee, and tumors, are common pathologies that lead to these chronic pain conditions. Axonal dieback induced by capsaicin (Caps) denervation has been FDA-approved to treat painful neuropathies and knee osteoarthritis but with shortterm efficacy and discomfort. Herein, we propose to evaluate pyridoxine (Pyr), vincristine sulfate (Vcr), and ionomycin (Imy) as axonal dieback compounds for denervation with potential to alleviate pain. Previous literature suggests Pyr, Vcr, and Imy can cause undesired axonal degeneration, but no previous work has evaluated axonal dieback and cytotoxicity …


Kinetics-Based Inference Of Environment-Dependent Microbial Interactions And Their Dynamic Variation, Hyun-Seob Song, Na-Rae Lee, Aimee K. Kessell, Hugh C. Mccullough, Seo-Young Park, Kang Zhou, Dong-Yup Lee Apr 2024

Kinetics-Based Inference Of Environment-Dependent Microbial Interactions And Their Dynamic Variation, Hyun-Seob Song, Na-Rae Lee, Aimee K. Kessell, Hugh C. Mccullough, Seo-Young Park, Kang Zhou, Dong-Yup Lee

Department of Biological Systems Engineering: Papers and Publications

Microbial communities in nature are dynamically evolving as member species change their interactions subject to environmental variations. Accounting for such context-dependent dynamic variations in interspecies interactions is critical for predictive ecological modeling. In the absence of generalizable theoretical foundations, we lack a fundamental understanding of how microbial interactions are driven by environmental factors, significantly limiting our capability to predict and engineer community dynamics and function. To address this issue, we propose a novel theoretical framework that allows us to represent interspecies interactions as an explicit function of environmental variables (such as substrate concentrations) by combining growth kinetics and a generalized …


Image Filtering To Improve Maize Tassel Detection Accuracy Using Machine Learning Algorithms, Eric Rodene, Gayara Demini Fernando, Ved Piyush, Yufeng Ge, James C. Schnable, Souparno Ghosh, Jinliang Yang Mar 2024

Image Filtering To Improve Maize Tassel Detection Accuracy Using Machine Learning Algorithms, Eric Rodene, Gayara Demini Fernando, Ved Piyush, Yufeng Ge, James C. Schnable, Souparno Ghosh, Jinliang Yang

Department of Biological Systems Engineering: Papers and Publications

Unmanned aerial vehicle (UAV)-based imagery has become widely used to collect timeseries agronomic data, which are then incorporated into plant breeding programs to enhance crop improvements. To make efficient analysis possible, in this study, by leveraging an aerial photography dataset for a field trial of 233 different inbred lines from the maize diversity panel, we developed machine learning methods for obtaining automated tassel counts at the plot level. We employed both an object-based counting-by-detection (CBD) approach and a density-based counting-by-regression (CBR) approach. Using an image segmentation method that removes most of the pixels not associated with the plant tassels, the …


Composting Reduces The Risks Of Resistome In Beef Cattle Manure At The Transcriptional Level, Yuepeng Sun, Zachery R. Staley, Bryan Woodbury, Jean-Jack Riethoven, Xu Li Mar 2024

Composting Reduces The Risks Of Resistome In Beef Cattle Manure At The Transcriptional Level, Yuepeng Sun, Zachery R. Staley, Bryan Woodbury, Jean-Jack Riethoven, Xu Li

Department of Biological Systems Engineering: Papers and Publications

Transcriptomic evidence is needed to determine whether composting is more effective than conventional stockpiling in mitigating the risk of resistome in livestock manure. The objective of this study is to compare composting and stockpiling for their effectiveness in reducing the risk of antibiotic resistance in beef cattle manure. Samples collected from the center and the surface of full-size manure stockpiling and composting piles were subject to metagenomic and metatranscriptomic analyses. While the distinctions in resistome between stockpiled and composted manure were not evident at the DNA level, the advantages of composting over stockpiling were evident at the transcriptomic level in …


A Systematic Review Of Fourth Industrial Revolution Technologies In Smart Irrigation: Constraints, Opportunities, And Future Prospects For Sub-Saharan Africa, Joshua Wanyama, Erion Bwambale, Shafik Kiraga, Abia Katimbo, Prossie Nakawuka, Isa Kabenge, Isaac Oluk Feb 2024

A Systematic Review Of Fourth Industrial Revolution Technologies In Smart Irrigation: Constraints, Opportunities, And Future Prospects For Sub-Saharan Africa, Joshua Wanyama, Erion Bwambale, Shafik Kiraga, Abia Katimbo, Prossie Nakawuka, Isa Kabenge, Isaac Oluk

Department of Biological Systems Engineering: Papers and Publications

The adoption of Fourth Industrial Revolution (4IR) technologies has revolutionized agricultural practices worldwide. However, their application in the context of sub-Saharan Africa remains a critical challenge. This study presents a systematic review that investigates the potential of 4IR technologies in smart irrigation. Sub- Saharan Africa faces multiple agricultural challenges, exacerbated by climate change, water scarcity, and inefficient irrigation practices. The need for sustainable, water-efficient, and data-driven irrigation systems is urgent to ensure food security and economic development in the region. This study addresses a crucial knowledge gap by assessing the constraints, opportunities, and prospects of implementing 4IR technologies for smart …


Impacts Of Farrowing Pen Design, Season, And Sow Parity On Litter Performance And Piglet Mortality, Verônica Madeira Pacheco, Tami M. Brown-Bandl, Gary A. Rohrer, Rafael Vieira De Sousa, Luciane Silva Martello Jan 2024

Impacts Of Farrowing Pen Design, Season, And Sow Parity On Litter Performance And Piglet Mortality, Verônica Madeira Pacheco, Tami M. Brown-Bandl, Gary A. Rohrer, Rafael Vieira De Sousa, Luciane Silva Martello

Department of Biological Systems Engineering: Papers and Publications

Piglet mortality during lactation is a significant concern in swine production, influenced by complex interactions involving sow, piglet, environmental, and management factors. While crushing by the sow may be the ultimate cause of piglet mortality, there are many factors influencing the outcome, including parity, thermal stress, and animal housing systems. New farrowing systems are continuously being developed; however, it is difficult for producers to make decisions without any scientific basis. This study aimed to assess the impact of different farrowing pen layouts on piglet performance, considering parity and season. A total of 546 sows and 9123 piglets were monitored across …


Enhancing Estimation Of Cover Crop Biomass Using Field-Based High-Throughput Phenotyping And Machine Learning Models, Geng Bai, Katja Koehler-Cole, David Scoby, Vesh R. Thapa, Andrea D. Basche, Yufeng Ge Jan 2024

Enhancing Estimation Of Cover Crop Biomass Using Field-Based High-Throughput Phenotyping And Machine Learning Models, Geng Bai, Katja Koehler-Cole, David Scoby, Vesh R. Thapa, Andrea D. Basche, Yufeng Ge

Department of Biological Systems Engineering: Papers and Publications

Incorporating cover crops into cropping systems offers numerous potential benefits, including the reduction of soil erosion, suppression of weeds, decreased nitrogen requirements for subsequent crops, and increased carbon sequestration. The aboveground biomass (AGB) of cover crops strongly influences their performance in delivering these benefits. Despite the significance of AGB, a comprehensive field-based high-throughput phenotyping study to quantify AGB of multiple cover crops in the U.S. Midwest has not been found. This study presents a two-year field experiment carried out in Eastern Nebraska, USA, to estimate AGB of five different cover crop species [canola (Brassica napus L.), rye (Secale …


Stage And Discharge Prediction From Documentary Time-Lapse Imagery, Kenneth W. Chapman, Troy E. Gilmore, Mehrube Mehrubeoglu, Christian D. Chapman, Aaron R. Mittelstet, John E. Stranzl Jr. Jan 2024

Stage And Discharge Prediction From Documentary Time-Lapse Imagery, Kenneth W. Chapman, Troy E. Gilmore, Mehrube Mehrubeoglu, Christian D. Chapman, Aaron R. Mittelstet, John E. Stranzl Jr.

Department of Biological Systems Engineering: Papers and Publications

Imagery from fixed, ground-based cameras is rich in qualitative and quantitative information that can improve stream discharge monitoring. For instance, time-lapse imagery may be valuable for filling data gaps when sensors fail and/or during lapses in funding for monitoring programs. In this study, we used a large image archive (> 40,000 images from 2012 to 2019) from a fixed, ground-based camera that is part of a documentary watershed imaging project (https://plattebasintimelapse.com/). Scalar image features were extracted from daylight images taken at one-hour intervals. The image features were fused with United States Geological Survey stage and discharge data as …


Life Cycle Greenhouse Gas Emissions In Maize No-Till Agroecosystems In Southern Brazil Based On A Long-Term Experiment, Guilherme Rosa Da Silva, Adam J. Liska, Cimelio Bayer Jan 2024

Life Cycle Greenhouse Gas Emissions In Maize No-Till Agroecosystems In Southern Brazil Based On A Long-Term Experiment, Guilherme Rosa Da Silva, Adam J. Liska, Cimelio Bayer

Department of Biological Systems Engineering: Papers and Publications

Brazilian agriculture is constantly questioned concerning its environmental impacts, particularly greenhouse gas (GHG) emissions. This research study used data from a 34-year field experiment to estimate the life cycle GHG emissions intensity of maize production for grain in farming systems under no-tillage (NT) and conventional tillage (CT) combined with Gramineae (oat) and legume (vetch) cover crops in southern Brazil. We applied the Feedstock Carbon Intensity Calculator for modeling the “field-to-farm gate” emissions with measured annual soil N2O and CH4 emissions data. For net CO2 emissions, increases in soil organic C (SOC) were applied as a proxy, …


Student Use Of Anchors And Metacognitive Strategies In Reflection, Anu Singh, Heidi A. Diefes-Dux Jan 2024

Student Use Of Anchors And Metacognitive Strategies In Reflection, Anu Singh, Heidi A. Diefes-Dux

Department of Biological Systems Engineering: Papers and Publications

Context: Self-regulation, a skillset involving taking charge of one’s own learning processes, is crucial for workplace success. Learners develop self-regulation skills through reflection where they recognize weaknesses and strengths by employing metacognitive strategies: planning, monitoring, and evaluating. Use of anchors assists learners’ engagement in reflection. Purpose or Goal: The purpose of this work was to gain insight into students’ use of anchors when reflecting on their learning. The two research questions: (1) To what extent do students link their self-evaluation and learning objective (LO) self-ratings to their reflections? and (2) What dimensions and level of metacognitive strategies do students use …


Classification Of Sow Postures Using Convolutional Neural Network And Depth Images, Md Towfiqur Rahman, Tami M. Brown-Brandl, Gary A. Rohrer, Sudhendu R. Sharma, Yeyin Shi Jan 2024

Classification Of Sow Postures Using Convolutional Neural Network And Depth Images, Md Towfiqur Rahman, Tami M. Brown-Brandl, Gary A. Rohrer, Sudhendu R. Sharma, Yeyin Shi

Department of Biological Systems Engineering: Papers and Publications

The United States swine industry reports an average preweaning mortality of approximately 16% where approximately 6% of them are attributed to piglets overlayed by sows. Detecting postural transitions and estimating sows’ time budgets for different postures are valuable information for breeders and engineering design of farrowing facilities to eventually reduce piglet death. Computer vision tools can help monitor changes in animal posture accurately and efficiently. To create a more robust system and eliminate varying lighting issues within a day including daytime/ nighttime differences, there is an advantage to using depth cameras over digital cameras. In this study, a computer vision …


Red Cabbage Juice-Mediated Gut Microbiota Modulation Improves Intestinal Epithelial Homeostasis And Ameliorates Colitis, Emily Jean Wilson, Nagabhishek Sirpu Natesh, Parsa Ghadermazi, Ramesh Pothuraju, Dipakkumar R. Prajapati, Sanjit Pandey, Jussuf T. Kaifi, John R. Dodam, Jeffrey Bryan, Christian L. Lorson, Aude A. Watrelot, Jason M. Foster, Thomas J. Mansell, Siu Hung Joshua Chan, Surinder K. Batra, Jeyamkondan Subbiah, Satyanarayana Rachagani Dec 2023

Red Cabbage Juice-Mediated Gut Microbiota Modulation Improves Intestinal Epithelial Homeostasis And Ameliorates Colitis, Emily Jean Wilson, Nagabhishek Sirpu Natesh, Parsa Ghadermazi, Ramesh Pothuraju, Dipakkumar R. Prajapati, Sanjit Pandey, Jussuf T. Kaifi, John R. Dodam, Jeffrey Bryan, Christian L. Lorson, Aude A. Watrelot, Jason M. Foster, Thomas J. Mansell, Siu Hung Joshua Chan, Surinder K. Batra, Jeyamkondan Subbiah, Satyanarayana Rachagani

Department of Biological Systems Engineering: Papers and Publications

Gut microbiota plays a crucial role in inflammatory bowel diseases (IBD) and can potentially prevent IBD through microbial-derived metabolites, making it a promising therapeutic avenue. Recent evidence suggests that despite an unclear underlying mechanism, red cabbage juice (RCJ) alleviates Dextran Sodium Sulfate (DSS)-induced colitis in mice. Thus, the study aims to unravel the molecular mechanism by which RCJ modulates the gut microbiota to alleviate DSS-induced colitis in mice. Using C57BL/6J mice, we evaluated RCJ’s protective role in DSS-induced colitis through two cycles of 3% DSS. Mice were daily gavaged with PBS or RCJ until the endpoint, and gut microbiota composition …


Determining The Presence And Size Of Shoulder Lesions In Sows Using Computer Vision, Shubham Bery, Tami M. Brown-Bandl, Bradley T. Jones, Gary A. Rohrer, Sudhendu Raj Sharma Dec 2023

Determining The Presence And Size Of Shoulder Lesions In Sows Using Computer Vision, Shubham Bery, Tami M. Brown-Bandl, Bradley T. Jones, Gary A. Rohrer, Sudhendu Raj Sharma

Department of Biological Systems Engineering: Papers and Publications

Shoulder sores predominantly arise in breeding sows and often result in untimely culling. Reported prevalence rates vary significantly, spanning between 5% and 50% depending upon the type of crate flooring inside a farm, the animal’s body condition, or an existing injury that causes lameness. These lesions represent not only a welfare concern but also have an economic impact due to the labor needed for treatment and medication. The objective of this study was to evaluate the use of computer vision techniques in detecting and determining the size of shoulder lesions. A Microsoft Kinect V2 camera captured the top-down depth and …


Deep Learning Models To Predict Finishing Pig Weight Using Point Clouds, Shiva Paudel, Rafael Vieira De Sousa, Sudhendu Raj Sharma, Tami M. Brown-Bandl Dec 2023

Deep Learning Models To Predict Finishing Pig Weight Using Point Clouds, Shiva Paudel, Rafael Vieira De Sousa, Sudhendu Raj Sharma, Tami M. Brown-Bandl

Department of Biological Systems Engineering: Papers and Publications

The selection of animals to be marketed is largely completed by their visual assessment, solely relying on the skill level of the animal caretaker. Real-time monitoring of the weight of farm animals would provide important information for not only marketing, but also for the assessment of health and well-being issues. The objective of this study was to develop and evaluate a method based on 3D Convolutional Neural Network to predict weight from point clouds. Intel Real Sense D435 stereo depth camera placed at 2.7 m height was used to capture the 3D videos of a single finishing pig freely walking …


Deep Learning Models To Predict Finishing Pig Weight Using Point Clouds, Shiva Paudel, Rafael Vieira De Sousa, Sudhendu Raj Sharma, Tami M. Brown-Bandl Dec 2023

Deep Learning Models To Predict Finishing Pig Weight Using Point Clouds, Shiva Paudel, Rafael Vieira De Sousa, Sudhendu Raj Sharma, Tami M. Brown-Bandl

Department of Biological Systems Engineering: Papers and Publications

The selection of animals to be marketed is largely completed by their visual assessment, solely relying on the skill level of the animal caretaker. Real-time monitoring of the weight of farm animals would provide important information for not only marketing, but also for the assessment of health and well-being issues. The objective of this study was to develop and evaluate a method based on 3D Convolutional Neural Network to predict weight from point clouds. Intel Real Sense D435 stereo depth camera placed at 2.7 m height was used to capture the 3D videos of a single finishing pig freely walking …


Impact Of Irrigation On Vulnerability Of Winter Wheat Under Extreme Climate Change Scenario: A Case Study Of North China Plain, Yulian Gao, Lin Wang, Yaojie Yue Dec 2023

Impact Of Irrigation On Vulnerability Of Winter Wheat Under Extreme Climate Change Scenario: A Case Study Of North China Plain, Yulian Gao, Lin Wang, Yaojie Yue

Department of Biological Systems Engineering: Papers and Publications

An inadequate understanding of the impacts of adaptation countermeasures tends to exaggerate the adverse e􀀀ects of climate change on agricultural systems. Motivated by proposing reasonable climate change adaptation countermeasures, the present study applied the EPIC model to quantify the impacts of climate change and irrigation changes with future socioeconomic development on agricultural production. Winter wheat yield losses using dynamic irrigation parameters in the North China Plain (NCP) from 2010 to 2099 under a scenario coupling climate change and future socioeconomic development (RCP8.5-SSP3), and those under an extreme climate change scenario (RCP8.5), were simulated. Results show that EPIC model demonstrates superior …


Systematic Comparison Of Nonviral Gene Delivery Strategies For Efficient Co-Expression Of Two Transgenes In Human Mesenchymal Stem Cells, Tyler Kozisek, Luke Samuelson, Andrew Hamann, Angela K. Pannier Dec 2023

Systematic Comparison Of Nonviral Gene Delivery Strategies For Efficient Co-Expression Of Two Transgenes In Human Mesenchymal Stem Cells, Tyler Kozisek, Luke Samuelson, Andrew Hamann, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Background Human mesenchymal stem cells (hMSCs) are being researched for cell-based therapies due to a host of unique properties, however, genetic modification of hMSCs, accomplished through nonviral gene delivery, could greatly advance their therapeutic potential. Furthermore, expression of multiple transgenes in hMSCs could greatly advance their clinical significance for treatment of multifaceted diseases, as individual transgenes could be expressed that target separate pathogenic drivers of complex diseases. Expressing multiple transgenes can be accomplished by delivering multiple DNA vectors encoding for each transgene, or by delivering a single poly-cistronic vector that encodes for each transgene and accomplishes expression through either use …


Aicropcam: Deploying Classification, Segmentation, Detection, And Counting Deep-Learning Models For Crop Monitoring On The Edge, Nipuna Chamara, Geng (Frank) Bai, Yufeng Ge Dec 2023

Aicropcam: Deploying Classification, Segmentation, Detection, And Counting Deep-Learning Models For Crop Monitoring On The Edge, Nipuna Chamara, Geng (Frank) Bai, Yufeng Ge

Department of Biological Systems Engineering: Papers and Publications

Precision Agriculture (PA) promises to meet the future demands for food, feed, fiber, and fuel while keeping their production sustainable and environmentally friendly. PA relies heavily on sensing technologies to inform site-specific decision supports for planting, irrigation, fertilization, spraying, and harvesting. Traditional point-based sensors enjoy small data sizes but are limited in their capacity to measure plant and canopy parameters. On the other hand, imaging sensors can be powerful in measuring a wide range of these parameters, especially when coupled with Artificial Intelligence. The challenge, however, is the lack of computing, electric power, and connectivity infrastructure in agricultural fields, preventing …


Determining The Presence And Size Of Shoulder Lesions In Sows Using Computer Vision, Shubham Bery, Tami M. Brown-Brandl, Bradley T. Jones, Gary A. Rohrer, Sudhendu Raj Sharma Dec 2023

Determining The Presence And Size Of Shoulder Lesions In Sows Using Computer Vision, Shubham Bery, Tami M. Brown-Brandl, Bradley T. Jones, Gary A. Rohrer, Sudhendu Raj Sharma

Department of Biological Systems Engineering: Papers and Publications

Shoulder sores predominantly arise in breeding sows and often result in untimely culling. Reported prevalence rates vary significantly, spanning between 5% and 50% depending upon the type of crate flooring inside a farm, the animal’s body condition, or an existing injury that causes lameness. These lesions represent not only a welfare concern but also have an economic impact due to the labor needed for treatment and medication. The objective of this study was to evaluate the use of computer vision techniques in detecting and determining the size of shoulder lesions. A Microsoft Kinect V2 camera captured the top-down depth and …


A Text Analytics Model For Agricultural Knowledge Discovery And Sustainable Food Production: A Case Study From Oklahoma Panhandle, Ali Bagheri, Saleh Taghvaeian, Dursun Delen Oct 2023

A Text Analytics Model For Agricultural Knowledge Discovery And Sustainable Food Production: A Case Study From Oklahoma Panhandle, Ali Bagheri, Saleh Taghvaeian, Dursun Delen

Department of Biological Systems Engineering: Papers and Publications

With recent increases in the use of social media in agricultural communities, many farmers are showing more and more interest in participating in social media and sharing different aspects of their profession with peers and policymakers. However, researchers have not explored this valuable data source well enough to help improve agribusiness decision-making. This study aims to investigate the potential capability and richness of social media for agricultural knowledge discovery, which can help monitor, detect, and predict critical agricultural events and activities and develop more sustainable food production and agricultural economy. This research utilizes text-mining tools and techniques to collect, process, …


Statistical And Machine Learning Approaches To Describe Factors Affecting Preweaning Mortality Of Piglets, Md Towfiqur Rahman, Tami M. Brown-Brandl, Gary A. Rohrer, Sudhendu R. Sharma, Vamsi Manthena, Yeyin Shi Oct 2023

Statistical And Machine Learning Approaches To Describe Factors Affecting Preweaning Mortality Of Piglets, Md Towfiqur Rahman, Tami M. Brown-Brandl, Gary A. Rohrer, Sudhendu R. Sharma, Vamsi Manthena, Yeyin Shi

Department of Biological Systems Engineering: Papers and Publications

High preweaning mortality (PWM) rates for piglets are a significant concern for the worldwide pork industries, causing economic loss and well-being issues. This study focused on identifying the factors affecting PWM, overlays, and predicting PWM using historical production data with statistical and machine learning models. Data were collected from 1,982 litters from the United States Meat Animal Research Center, Nebraska, over the years 2016 to 2021. Sows were housed in a farrowing building with three rooms, each with 20 farrowing crates, and taken care of by well-trained animal caretakers. A generalized linear model was used to analyze the various sow, …


Statistical And Machine Learning Approaches To Describe Factors Affecting Preweaning Mortality Of Piglets, Md Towfiqur Rahman, Tami M. Brown-Bandl, Gary A. Rohrer, Sudhendu R. Sharma, Vamsi Manthena, Yeyin Shi Oct 2023

Statistical And Machine Learning Approaches To Describe Factors Affecting Preweaning Mortality Of Piglets, Md Towfiqur Rahman, Tami M. Brown-Bandl, Gary A. Rohrer, Sudhendu R. Sharma, Vamsi Manthena, Yeyin Shi

Department of Biological Systems Engineering: Papers and Publications

High preweaning mortality (PWM) rates for piglets are a significant concern for the worldwide pork industries, causing economic loss and well-being issues. This study focused on identifying the factors affecting PWM, overlays, and predicting PWM using historical production data with statistical and machine learning models. Data were collected from 1,982 litters from the U.S. Meat Animal Research Center, Nebraska, over the years 2016 to 2021. Sows were housed in a farrowing building with three rooms, each with 20 farrowing crates, and taken care of by well-trained animal caretakers. A generalized linear model was used to analyze the various sow, litter, …


Statistical And Machine Learning Approaches To Describe Factors Affecting Preweaning Mortality Of Piglets, Md Towfiqur Rahman, Tami M. Brown-Bandl, Gary A. Rohrer, Sudhendu R. Sharma, Vamsi Manthena, Yeyin Shi Oct 2023

Statistical And Machine Learning Approaches To Describe Factors Affecting Preweaning Mortality Of Piglets, Md Towfiqur Rahman, Tami M. Brown-Bandl, Gary A. Rohrer, Sudhendu R. Sharma, Vamsi Manthena, Yeyin Shi

Department of Biological Systems Engineering: Papers and Publications

High preweaning mortality (PWM) rates for piglets are a significant concern for the worldwide pork industries, causing economic loss and well-being issues. This study focused on identifying the factors affecting PWM, overlays, and predicting PWM using historical production data with statistical and machine learning models. Data were collected from 1,982 litters from the U.S. Meat Animal Research Center, Nebraska, over the years 2016 to 2021. Sows were housed in a farrowing building with three rooms, each with 20 farrowing crates, and taken care of by well-trained animal caretakers. A generalized linear model was used to analyze the various sow, litter, …


Upscaling Wetland Methane Emissions From The Fluxnet-Ch4 Eddy Covariance Network (Upch4 V1.0): Model Development, Network Assessment, And Budget Comparison, Gavin Mcnicol, Etienne Fluet-Chouinard, Zutao Ouyang, Sara Knox, Zhen Zhang, Tuula Aalto, Sheel Bansal, Kuang Yu Chang, Min Chen, Kyle Delwiche, Sarah Feron, Mathias Goeckede, Jinxun Liu, Avni Malhotra, Joe R. Melton, William Riley, Rodrigo Vargas, Kunxiaojia Yuan, Qing Ying, Qing Zhu, Pavel Alekseychik, Mika Aurela, David Billesbach, David I. Campbell, Jiquan Chen, Housen Chu, Ankur R. Desai, Eugenie Euskirchen, Jordan Goodrich, Timothy Griffis, Manuel Helbig, Takashi Hirano, Hiroki Iwata, Gerald Jurasinski, John King, Franziska Koebsch, Randall Kolka, Ken Krauss, Annalea Lohila, Ivan Mammarella Oct 2023

Upscaling Wetland Methane Emissions From The Fluxnet-Ch4 Eddy Covariance Network (Upch4 V1.0): Model Development, Network Assessment, And Budget Comparison, Gavin Mcnicol, Etienne Fluet-Chouinard, Zutao Ouyang, Sara Knox, Zhen Zhang, Tuula Aalto, Sheel Bansal, Kuang Yu Chang, Min Chen, Kyle Delwiche, Sarah Feron, Mathias Goeckede, Jinxun Liu, Avni Malhotra, Joe R. Melton, William Riley, Rodrigo Vargas, Kunxiaojia Yuan, Qing Ying, Qing Zhu, Pavel Alekseychik, Mika Aurela, David Billesbach, David I. Campbell, Jiquan Chen, Housen Chu, Ankur R. Desai, Eugenie Euskirchen, Jordan Goodrich, Timothy Griffis, Manuel Helbig, Takashi Hirano, Hiroki Iwata, Gerald Jurasinski, John King, Franziska Koebsch, Randall Kolka, Ken Krauss, Annalea Lohila, Ivan Mammarella

Department of Biological Systems Engineering: Papers and Publications

Wetlands are responsible for 20%–31% of global methane (CH4) emissions and account for a large source of uncertainty in the global CH4 budget. Data-driven upscaling of CH4 fluxes from eddy covariance measurements can provide new and independent bottom-up estimates of wetland CH4 emissions. Here, we develop a six-predictor random forest upscaling model (UpCH4), trained on 119 site-years of eddy covariance CH4 flux data from 43 freshwater wetland sites in the FLUXNET-CH4 Community Product. Network patterns in site-level annual means and mean seasonal cycles of CH4 fluxes were reproduced accurately in tundra, boreal, and temperate regions (Nash-Sutcliffe Efficiency ∼0.52–0.63 and 0.53). …


Resilin Distribution And Abundance In Apis Mellifera Across Biological Age Classes And Castes, Audrey Anderson, Noah Keime, Chandler Fong, Andrew Kraemer, Carol Fassbinder-Orth Sep 2023

Resilin Distribution And Abundance In Apis Mellifera Across Biological Age Classes And Castes, Audrey Anderson, Noah Keime, Chandler Fong, Andrew Kraemer, Carol Fassbinder-Orth

Department of Biological Systems Engineering: Papers and Publications

The presence of resilin, an elastomeric protein, in insect vein joints provides the flexible, passive deformations that are crucial to flapping flight. This study investigated the resilin gene expression and autofluorescence dynamics among Apis mellifera (honey bee) worker age classes and drone honey bees. Resilin gene expression was determined via ddPCR on whole honey bees and resilin autofluorescence was measured in the 1m-cu, 2m-cu, Cu-V, and Cu2-V joints on the forewing and the Cu-V joint of the hindwing. Resilin gene expression varied significantly with age, with resilin activity being highest in the pupae. Autofluorescence of the 1m-cu and the Cu-V …


Influence Of Ultrasound Machine Settings On Quantitative Measures Derived From Spatial Frequency Analysis Of Muscle Tissue, Scott K. Crawford, Stephanie A. Kliethermes, Bryan C. Heiderscheit, Greg Bashford Aug 2023

Influence Of Ultrasound Machine Settings On Quantitative Measures Derived From Spatial Frequency Analysis Of Muscle Tissue, Scott K. Crawford, Stephanie A. Kliethermes, Bryan C. Heiderscheit, Greg Bashford

Department of Biological Systems Engineering: Papers and Publications

Background Ultrasound is a powerful tool for diagnostic purposes and provides insight into both normal and pathologic tissue structure. Spatial frequency analysis (SFA) methods characterize musculoskeletal tissue organization from ultrasound images. Both sonographers in clinical imaging and researchers may alter a minimized range of ultrasound settings to optimize image quality, and it is important to know how these small adjustments of these settings affect SFA parameters. The purpose of this study was to investigate the effects of making small adjustments in a typical default ultrasound machine setting on extracted spatial frequency parameters (peak spatial frequency radius (PSFR), Mmax, Mmax%, and …