Digital Commons Network^™

Predicting Successful Chaperoning Of Fabry Disease Mutants Via Computation, Priyank Patel

Masters Theses

Fabry disease is an inherited X-linked recessive disorder caused by mutations in the galactosidase alpha (GLA) gene, leading to deficiencies in α-galactosidase A (α-GAL) enzyme production. α-GAL, a lysosomal glycosidase, catalyzes the removal of a terminal α-galactose; however, loss of α-GAL activity leads to accumulation of globotriaosylceramide (an endogenous substrate) and the eventual onset of the disease. Approved treatments for Fabry disease include enzyme replacement therapy and pharmacological chaperone therapy. In the latter treatment, 1-deoxygalactonojirimycin (DGJ), a pharmacological chaperone, is administered to Fabry disease patients, leading to increased enzymatic activity. The DGJ iminosugar acts as a competitive inhibitor …

Reconsidering Diasporic Literature: "Homeland" And "Otherness" In The Lost Daughter Of Happiness, Qijun Zhou

Masters Theses

This thesis examines the transformation of “homeland” and “otherness” as well as the relationship between each other in The Lost Daughter of Happiness （扶桑 Fusang). I begin by exploring how the migration of Chinese to the United States is depicted as an endless trajectory in the story through a historical engagement and a dialogue between two generations. From there, I plan to point out that the story complicates the meaning of diaspora as it can not only represent a spatial dislocation, but also a temporal dislocation. Thus, I argue that it destabilizes the conventional ideology which refers “homeland” to a …

An Examination Of Tern Diet In A Changing Gulf Of Maine, Keenan Yakola

Masters Theses

The Gulf of Maine is a dynamic ecosystem with rapidly warming sea surface temperatures (SSTs), therefore it is vital to understand how species interactions vary over time and space. In chapter two, I quantify and compare dietary differences among four tern species, across seven islands in the region, over a 32-year period. Multivariate statistical analyses were employed to discern spatial and temporal differences in foraging ecology. Findings suggest there are significant differences between species and islands; however, only three prey species comprise the majority of chick diet for all terns and islands. The reliance on only a few prey items …

“Nothing Material Occurred”: The Maritime Captures That Caused Then Outlasted The United States’ Quasi War With France, Emma Zeig

Masters Theses

This thesis examines the French maritime seizures during the eighteenth-century US Quasi War with France (also called the half war, or the United States’ undeclared war with France), encompassing events on both sides of the Atlantic Ocean, in France, the United States, and the Caribbean, particularly Haiti. The analysis focuses on the captured ships, telling the stories of seamen who feared for their lives and merchants who lost their ships. This point of view allows the thesis to explore an area of the Quasi War that are less documented in other histories: how civilian participants experienced violence and the indifference …

Envisioning Pathways Toward Transformative Food Systems Change: Understanding The Role Of Multi-Stakeholder Engagement At The Culinary And Nutrition Center In Springfield, Ma, Kristen Whitmore

Masters Theses

The alternative food movement claims varied goals such as building environmental sustainability, strengthening local economies, and promoting health equity, yet critics argue that the movement’s transformative potential is threatened by a lack of shared vision. Literature suggests that community-based multi-stakeholder coalitions are a useful tool for building consensus around food systems futures. But what kinds of futures? Home Grown Springfield is a school food initiative aimed at reducing hunger in Springfield, MA by serving healthy, homemade, and locally-sourced meals via the Culinary and Nutrition Center, a brand-new full-service commercial kitchen and storage facility. This qualitative case study examines the engagement …

The Role Of International River Basin Organizations In Facilitating Science Use In Policy, Kelsey Wentling

Masters Theses

Transboundary watershed management seeks to reconcile the dichotomy between political lines and the resources that flow freely over such borders. Transboundary waters cover half of the earth’s surface and define the natural communities of over 40% of the global population. Because water plays an integral role in every culture and society, international entities seek to identify the principles and methods that minimize conflict and maximize harmonious water resource management across borders. Successful management practices to date have aimed to incorporate relevant scientific literature throughout the basin using alternate governance structures. International River Basin Organizations (IRBOs), independent governing structures, provide one …

Investigating The Role Of Lbh During Early Embryonic Development In Xenopus Laevis, Emma Weir

Masters Theses

LBH is a highly conserved protein whose role during vertebrate development is relatively under-studied. In collaboration with the Albertson lab, our lab has previously shown that it is necessary for cranial neural crest cell migration in the zebrafish and in Xenopus laevis. The molecular mechanisms through which it acts are not well understood.

In Xenopus, LBH is a maternally deposited protein. As such, studying its role in early development has not been feasible through the morpholino-mediated knockdown techniques that prevent translation of target genes. Recently, a technique for degrading endogenous proteins was developed, called Trim-Away. This was developed in mammalian …

How Perception Of Decision Environment And Future Information Affects Changes In Delay Discounting Rates: Differences Across U.S. And China, Differences Before And After The U.S. 2018 Midterm Elections, Fran Walsh

Masters Theses

In this thesis, I will explore the idea that choices between present, smaller value options and future, larger value options depend on how much individuals trust the future to deliver the reward. Due to this aspect of trust, the individual must build their estimate of trust based on information for their present environment and their future expectations. This estimate of future trust can change across different time points in the same environment (i.e., before and after a national election) and between environments in the same time point (i.e., between two countries experiencing different economic rates of change). In this set …

Characterizing The Interaction Between Non-Pathogenic Fusarium Oxysporum And Arabidopsis Thaliana To Determine Beneficial Effects Conferred To The Model Plant Host, Kathryn Isabelle Vescio

Masters Theses

Fusarium oxysporum (Fo) is a soil-borne fungal pathogen that causes vascular wilt disease on a broad range of plants, including agricultural crops and the model plant Arabidopsis thaliana. There are non-pathogenic members of the Fo species complex that confer defense benefits against other pathogens to the host plant, however alteration to the host’s physiology through interaction with one of these strains, Fo47, have not been described. In this study, we aimed to establish the Fo47-A. thaliana interaction and determine if Fo47 reduces disease severity of a pathogenic Fo isolate, Fo5176. Additionally, we sought to use bioinformatics to mine …

The Effects Of Acidosis On Calcium Dependent Binding Of A Single Crossbridge, Matthew Unger

Masters Theses

Intracellular acidosis is a putative agent of skeletal muscle fatigue, in part, because acidosis depresses the calcium (Ca²⁺) sensitivity and force production of muscle (18, 50). However, the molecular mechanisms behind this depression in Ca²⁺ sensitivity and force production are unknown. This gap in knowledge poses a significant challenge in generating a complete understanding of the fatigue process. To close this gap, the ability of myosin to bind to a single actin filament was measured under acidic conditions, in a laser trap assay, with and without regulatory proteins. Decreasing pH from 7.4 to 6.5 reduced the frequency …

A Novel Infield Metagenomic Approach To Evaluating Surface Water Quality In Lake Warner, Brooke Stebbins

Masters Theses

In January 2010, a magnitude 7.0 earthquake devastated Haiti, one of the poorest countries in the Western Hemisphere. Haiti’s weak sanitation infrastructure and limitations in the public health system made the country susceptible to the spread of waterborne diseases. Following the earthquake, cholera rapidly spread through Haiti, killing 4,672 people in 5 months and leaving thousands hospitalized (MSNBC, 2010). Cholera is an infectious diarrheal disease caused by the pathogen, Vibrio cholerae, which results in severe dehydration with a high mortality risk. The source of the epidemic was traced to the Artibonite River, the island’s longest and most essential drinking …

Calculation Of Scalar Isosurface Area And Applications, Kedar Prashant Shete

Masters Theses

The problem of calculating iso-surface statistics in turbulent flows is interesting for a number of reasons, some of them being combustion modeling, entrainment through turbulent/non-turbulent interfaces, calculating mass flux through iso-scalar surfaces and mapping of scalar fields. A fundamental effect of fuid turbulence is to wrinkle scalar iso-surfaces. A review of the literature shows that iso-surface calculations have primarily been done with geometric methods, which have challenges when used to calculate surfaces that have high complexity, such as in turbulent flows. In this thesis, we propose an alternative integral method and test it against analytical solutions. We present a parallelized …

Influence Of Emotion Processing And Affect Intensity On The Engagement Of Inhibitory Control In Young Adults With Attention-Deficit/Hyperactivity Disorder, Zachary Salander

Masters Theses

How individuals process different affective cues, as well as how intensely they experience different emotions, may influence how efficient they are at engaging inhibitory control. To date, it is unclear if these influences differ among young adults with and without ADHD. The current study tested the variation in young adults’ inhibitory control to three affective cues (i.e., fear, happy, and neutral) in an Emotion Go/Nogo task. Results suggest better inhibitory control in response to more distinct cues (i.e., fear Nogo/happy Go). The order in which cues were presented also mattered, such that participants displayed enhanced inhibitory control when first presented …

'Choose A Language Like A Wedding Ring': Polysystems, Norms And Pseudotranslation In Lea Goldberg’S Poetry & Prose, Benjamin Rangell

Masters Theses

Lea Goldberg [1911-1970] is one of modern Hebrew literature’s most significant poet/translators. This thesis approaches her early poetry and prose from the perspective of three theories of translation. ‘Polysystems’, ‘norms’, and ‘pseudotranslation’ grew from the scholarly and translation-lineage in Hebrew literary studies that Goldberg herself contributed to. Utilizing these three methods of reading, this thesis argues that translation’s thematization in Goldberg’s creative work is evidence for the poet’s ideal for a cosmopolitan, multilingual, national literature in the new Jewish State. This receptive stance to previous and concurrent literary traditions was met with much skepticism and criticism from Goldberg’s colleagues, and …

Behavior Of Prestressed Concrete Bridges With Closure Pour Connections And Diaphragms, Gercelino Ramos

Masters Theses

Accelerated Bridge Construction (ABC) has gained substantial popularity in new bridge construction and bridge deck replacement because it offers innovative construction techniques that result in time and cost savings when compared to traditional bridge construction practice. One technology commonly implemented in ABC to effectively execute its projects is the use of prefabricated bridge components (precast/prestressed bridge components). Precast/prestressed bridge components are fabricated offsite or near the site and then connected on-site using small volume closure pour connections. Diaphragms are also commonly used to strengthen the connection between certain prefabricated components used in ABC, such as beam elements. Bridges containing closure …

A Study On Controlling Power Supply Ramp-Up Time In Sram Pufs, Harshavardhan Ramanna

Masters Theses

With growing connectivity in the modern era, the risk of encrypted data stored in hardware being exposed to third-party adversaries is higher than ever. The security of encrypted data depends on the secrecy of the stored key. Conventional methods of storing keys in Non-Volatile Memory have been shown to be susceptible to physical attacks. Physically Unclonable Functions provide a unique alternative to conventional key storage. SRAM PUFs utilize inherent process variation caused during manufacturing to derive secret keys from the power-up values of SRAM memory cells.

This thesis analyzes the effect of supply ramp-up times on the reliability of SRAM …

The Promise Of Vr Headsets: Validation Of A Virtual Reality Headset-Based Driving Simulator For Measuring Drivers’ Hazard Anticipation Performance, Ganesh Pai Mangalore

Masters Theses

The objective of the current study is to evaluate the use of virtual reality (VR) headsets to measure driving performance. This is desirable because they are several orders of magnitude less expensive and, if validated, could greatly extend the powers of simulation. Out of several possible measures of performance that could be considered for evaluating VR headsets, the current study specifically examines drivers’ latent hazard anticipation behavior both because it has been linked to crashes and because it has been shown to be significantly poorer in young drivers compared to their experienced counterparts in traditional driving simulators and in open …

Topology Network Optimization Of Facility Planning And Design Problems, Ravi Ratan Raj Monga

Masters Theses

The research attempts to provide a graphical theory-based approach to solve the facility layout problem. Which has generally been approached using Quadratic Assignment Problem (QAP) in the past, an algebraic method. It is a very complex problem and is part of the NP-Hard optimization class, because of the nonlinear quadratic objective function and (0,1) binary variables. The research is divided into three phases which together provide an optimal facility layout, block plan solution consisting of MHS (material handling solution) projected onto the block plan. In phase one, we solve for the position of departments in a facility based on flow …

Relationships Between Personality Type And Cognitive Ability In Marmoset Monkeys (Callithrix Jacchus), Zachary Marciano

Masters Theses

Personality refers to multiple traits that are thought to be stable over time and across situations. It is recognized that personality has a neural basis and is associated with health outcomes. Whether personality is also associated with cognitive ability, however, is still a matter of intense debate. One way to examine these potential relationships is to use a nonhuman primate model for which complexities present in humans can be minimized. Recent research into the varying personality types of marmoset monkeys suggests that there are predominantly three to five core primary domains that most marmosets and other primates can be categorized …

Arboriculture Safety Around The World, Jamie Lim

Masters Theses

Arboricultural work is inherently dangerous, with more serious injuries and fatalities than most other professions. Safety standards exist in some jurisdictions, but it is unclear how many standards exist, how they compare to one another, and whether (and how many) jurisdictions share standards. To establish a baseline understanding of these issues, my objectives were to (i) develop a database of existing standards, (ii) identify the most frequently occurring safety topics and (iii) describe similarities and differences in safety topics among standards from different countries. I worked with a variety of contacts and traditional university library resources to identify, obtain, analyze, …

Application Of Flow Cytometry As Novel Technology In Studying Lipid Oxidation In Oil-In-Water Emulsions, Peilong Li

Masters Theses

The body of literature on the impact of emulsion particle size on oxidation rates is unclear. This could be because emulsions are typically polydisperse and the oxidation rate of individual droplets is impossible to discern. Flow cytometry is a technique for studying individual cells and their subpopulations using fluorescence technologies. It is possible that individual emulsion droplets could also be characterized by flow cytometry as a novel approach for studying lipid oxidation. Typical emulsion droplets are too small to be visualized by flow cytometer, so emulsions were prepared to have droplets > 2 μm; weighting agent and xanthan gum were added …

The Efficacy Of Atp Monitoring Devices At Measuring Organic Matter On Postharvest Surfaces, Kristin Lane

Masters Theses

The Food Safety Modernization Act (FSMA), specifically the Produce Safety Rule (PSR), requires growers to clean and sanitize food-contact surfaces to protect against produce contamination. The ATP monitoring device is a potential sanitation tool to monitor the efficacy of an on-farm cleaning and sanitation program that could help growers meet regulatory expectations mandated by PSR. The ATP device uses bioluminescence to detect all ATP (found in bacteria and produce matter cells) from a swabbed surface. Little work has been done to test the efficacy of these tools under postharvest conditions. The present study evaluated ATP measurement for postharvest surface cleanliness …

Using Visual Media To Empower Citizen Scientists: A Case Study Of The Outsmart App, Megan E. Kierstead

Masters Theses

To be successful citizen science projects need to do two key things: (1) they need to meaningfully engage the public and they must also provide people with the tools, expertise, and/or training needed to participate in rigorous research that can be used by the scientific community. In some ways, these requirements are potentially at odds. Emphasis on rigor and expertise risks excluding members of the public who do not feel qualified to participate in esoteric or technically difficult scientific research. Conversely, projects that eschew rigorous methods in favor of wider participation might lead to bad data that cannot be used …

The Morality Of Chinese Legalism: Han Fei’S Advanced Philosophy, Yuan Ke

Masters Theses

Legalism, as one of the most useful philosophies of government, has attracted a great deal of scholarly attention. The work of Han Fei—one of the most influential proponents of Legalism—has been scrutinized and critiqued for centuries as immoral. I intend to show Legalism, especially the Han Feizi, is moral through focusing on four aspects of Han Fei’s work. First, his understanding of human nature. Han Fei states people are born with a hatred of harm and a love of profit. This understanding of human nature can never lead to a cognitive distortions in governing. So it is a moral basic …

Patterns Of Morphological Plasticity In Metriaclima Zebra And Danio Rerio Suggest Differently Canalized Phenotypes Due To Form-Function Relationships, Dylan Jockel

Masters Theses

In order to ascertain the degree of compatibility in developmental restructuring and behavioral plasticity between two fish species frequently made subject of laboratory research (Metriaclima zebra & Danio rerio), alternative trophic niche exposure experiments utilizing novel three-prong feeding treatments were conducted to obtain morphometric data, which demonstrated both species do bear some degree of plasticity. The results are somewhat complicated by differences in locality of detectable restructuring, which may be due to disparity in the form-function relationship for each species’ lineage. Each is notable in the manner of respective species’ jaw protrusion, as it is driven by anterior …

Museum Design As A Tool For A City, Cunbei Jiang

Masters Theses

With the financial growth and international acclaim brought about by Guggenheim Museum for Bilbao, the media started to talk about the so-called “Bilbao Effect”. For the next two decades, the general public has been more and more convinced and accustomed to the positive results landmark architecture might brought for their cities. Thus it is worthwhile to explore the root of the Bilbao Effect and to dig into the effects of Guggenheim Museum so that similar industrial cities may select and apply strategies basing on their own conditions.

On The Fuel Spray Applications Of Multi-Phase Eulerian Cfd Techniques, Gabriel Lev Jacobsohn

Masters Theses

Eulerian-Eulerian Computational Fluid Dynamics (CFD) techniques continue to show promise for characterizing the internal flow and near-field spray for various fuel injection systems. These regions are difficult to observe experimentally, and simulations of such regions are limited by computational expense or reliance on empiricism using other methods. The physics governing spray atomization are first introduced. Impinging jet sprays and Gasoline Direct Injection (GDI) are selected as applications, and modern computational/experimental approaches to their study are reviewed. Two in-house CFD solvers are described and subsequently applied in several case studies. Accurate prediction of the liquid distribution in a like-doublet impinging jet …

Translation Issues In Modern Chinese Literature: Viewpoint, Fate And Metaphor In Xia Shang's "The Finger-Guessing Game", Jonathan Heinrichs

Masters Theses

The Finger-Guessing Game is a novel with many layers of themes, characterization, and metaphor, and conveying all of these varied aspects requires a detailed, careful approach to translation. With this thesis I aim to show that strictly adhering to a singular translation method, such as “word-for-word” or “sense-for-sense,” will produce unsatisfactory results at certain points within the novel. This is accomplished by an overview of several different unique aspects of the writing style of this novel, viewpoint, the theme of fate, and the use of idioms and metaphors. Following this will be an analysis of these aspects’ functions within the …

Estimating Age-Specific Contraceptive Use For Spacing Of Childbirth For All Countries In Sub-Saharan Africa From 1985 To 2030 Using A Bayesian Hierarchical Time Series Model, Gregory Guranich

Masters Theses

Contraceptive usage for spacing of childbirth is an important indicator for understanding family planning practices as well as fertility transitions. Fertility transition are especially important in sub-Saharan Africa where fertility remains high in many countries. However, estimates and short-term projections are generally not available for countries in this region. We developed a Bayesian hierarchical time series model to estimate and project usage of contraceptives for spacing by 5-year age groups for all countries in sub-Saharan Africa for the years 1985-2030. Estimating country-age-year specific usage is challenging due to limited data availability. We use Bayesian hierarchical models to share information across …

Sustainable Travel Incentives Optimization In Multimodal Networks, Hossein Ghafourian

Masters Theses

Tripod, an integrated bi-level transportation management system, is a smartphone application from the potential user’s point of view which would be instantly accessed prior to performing the trip. Since there are constantly several alternatives for any trip, Tripod considers a series and combination of various parameters, including departure time, mode and route, and rewards for each alternative with a number of redeemable points for goods and services called “Tokens”. The framework responsible for computing the optimized number of tokens awarded to the set of available alternatives in order to minimize the system-wide energy consumption under a constrained Token budget, is …