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Multiple Genetically Engineered Humanized Microenvironments In A Single Mouse, Jungwoo Lee, Dirk Heckl, Biju Parekkadan
Multiple Genetically Engineered Humanized Microenvironments In A Single Mouse, Jungwoo Lee, Dirk Heckl, Biju Parekkadan
Chemical Engineering Faculty Publication Series
Background
Immunodeficient mouse models that accept human cell and tissue grafts can contribute greater knowledge to human stem cell research. In this technical report, we used biomaterial implants seeded with genetically engineered stromal cells to create several unique microenvironments in a single mouse. The scope of study was focused on human CD34 hematopoietic stem/progenitor cell (HSPC) engraftment and differentiation within the engineered microenvironment.
Results
A mouse model system was created using subdermal implant sites that overexpressed a specific human cytokines (Vascular Endothelial Growth Factor A (hVEGFa), Stromal Derived Factor 1 Alpha (hSDF1a), or Tumor Necrosis Factor Alpha (hTNFa)) by stromal …