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Automated Solid-Substrate Cultivation Of The Anaerobic Bacterium Clostridium Thermocellum, Mathew J. Ruwaya
Automated Solid-Substrate Cultivation Of The Anaerobic Bacterium Clostridium Thermocellum, Mathew J. Ruwaya
Theses and Dissertations--Biosystems and Agricultural Engineering
The organism Clostridium thermocellum grows on cellulosic substrates and produces ethanol, acetate, lactate, formic acid, and CO2. The organic acids produced alter the growth environment in which the bacteria grows and ultimately inhibit bacterial growth. One method which has been used successfully to maintain the system at acceptable growth conditions is to intermittently flush out the spent media and metabolic products and replace with new fermentation media. Our goal was to design and build an automated system that will automatically flush the spent media from the growing culture and resupply new media without manual intervention. An automated control …
The Influence Of Media Ingredients And Ph On The Growth Of Chloromonas Rosae Var. Psychrophila, Caoli Zheng
The Influence Of Media Ingredients And Ph On The Growth Of Chloromonas Rosae Var. Psychrophila, Caoli Zheng
Theses and Dissertations--Biosystems and Agricultural Engineering
In order to utilize algae to fix CO2 and reduce Greenhouse gas emissions, large-scale, year-round algae cultivation will be required. This will require alga species that thrive in colder weather with media recipes based on commercially available fertilizers. The objectives of this study were to evaluate the effect of using commercial based media and the effect of pH when growing the cold-temperature algae Chloromonas rosae var. psychrophila. Commercial grade media ingredients urea, potash, triple superphosphate, and Sprint 330® were used to provide nitrogen, potassium, phosphorus, and EDTA with iron, respectively. The commercial grade media supported growth similar to the growth …
Up Regulation Of Heat Shock Protein 70b (Hsp70b) And Ssa1 In Chlamydomonas Reinhardtii Via Hsp70a-Rbcs2 And Psad Promoter, B. Kirtley Amos
Up Regulation Of Heat Shock Protein 70b (Hsp70b) And Ssa1 In Chlamydomonas Reinhardtii Via Hsp70a-Rbcs2 And Psad Promoter, B. Kirtley Amos
Theses and Dissertations--Biosystems and Agricultural Engineering
Fabrication of effective algae cultivation systems adjacent to coal-fired power plants to fixate waste CO2 would represent a sizable step towards achieving a carbon neutral energy cycle. However, emission gas would elevate the algal cultivation system temperature and decreases its pH without expensive preprocessing. Increased temperature and acidity constitutes a profound stress on the algae. Although stressed algae produce heat shock proteins (HSPs) that promote protein folding and protect against stress, the ordinary biological response is insufficient to protect against coal flue gas. Experimental upregulation of HSPs could make algae respond to the stress caused by high temperatures and …
Evaluation Of Heat Shock Protein 70a (Hsp70a) In Chlamydomonas Reinhardtii, Sarah Nicole Short
Evaluation Of Heat Shock Protein 70a (Hsp70a) In Chlamydomonas Reinhardtii, Sarah Nicole Short
Theses and Dissertations--Biosystems and Agricultural Engineering
Algae are being considered as a possible tool for carbon dioxide mitigation because they uptake carbon dioxide during photosynthesis. Using flue gas from a coal-fired power plant as a carbon source would allow the algae to remove CO2 from the flue gas before it is emitted into the atmosphere. Because algae do not grow well at the high temperature, low pH conditions presented by flue gas, the traditional approach has been to alter the flue gas to suit the needs of the algae; however, this work aimed to genetically modify the algae Chlamydomonas reinhardtii to grow better at less …