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Fractionation And Characterization Of Lignin Streams From Genetically Engineered Switchgrass, Enshi Liu
Fractionation And Characterization Of Lignin Streams From Genetically Engineered Switchgrass, Enshi Liu
Theses and Dissertations--Biosystems and Agricultural Engineering
Development of biomass feedstocks with desirable traits for cost-effective conversion is one of the main focus areas in biofuels research. As suggested by techno-economic analyses, the success of a lignocellulose-based biorefinery largely relies on the utilization of lignin to generate value-added products, i.e. fuels and chemicals. The fate of lignin and its structural/compositional changes during pretreatment have received increasing attention; however, the effect of genetic modification on the fractionation, depolymerization and catalytic upgrading of lignin from genetically engineered plants is not well understood. This study aims to fractionate and characterize the lignin streams from a wild-type and two genetically engineered …
Recirculating Calcium Hydroxide Solution: A Practical Choice For On-Farm High Solids Lignocellulose Pretreatment, William S. Sympson Jr.
Recirculating Calcium Hydroxide Solution: A Practical Choice For On-Farm High Solids Lignocellulose Pretreatment, William S. Sympson Jr.
Theses and Dissertations--Biosystems and Agricultural Engineering
Pretreatment is a necessary step in the utilization of lignocellulosic biomass for biochemical conversion to higher value products. There are multiple chemical choices for industrial settings, however on-farm choices are constrained to near ambient conditions with minimal specialized equipment, training, and limited waste disposal. Calcium hydroxide (lime) is suitable for on-farm use. This work presents the novel idea of pretreating biomass by recirculating a filtered, saturated lime solution in an up-flow, high solids (14-16% w/w) configuration at ambient conditions. In this system, lime solids were efficiently consumed, post-pretreatment washing of substrate did not significantly improve glucose yields, and energy and …
Field Implementation Of Phanerochaete Chrysosporium Biomass Pretreatment: Fungal Identification And Inoculation Techniques, Bobby D. Carey Jr
Field Implementation Of Phanerochaete Chrysosporium Biomass Pretreatment: Fungal Identification And Inoculation Techniques, Bobby D. Carey Jr
Theses and Dissertations--Biosystems and Agricultural Engineering
Scaling biological pretreatment from the bench scale to the production scale may be more economical if unsterilized feedstock are used, however these allow for microbial competition from contaminates. An accurate and rapid method for identifying the desired biological pretreatment organism is necessary to confirm the presence of the desired organism when contaminates are morphologically similar to the target organism. Traditional methods, such as visual identification, sequencing, and selective plating can be time consuming and are sometimes still inconclusive. Based on methods described in the literature, plasmid DNA containing the marker genes gus (�-glucuronidase), LacZ, and gfp (green fluorescence protein) …