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Expression Of Ipt Gene Controlled By An Ethylene And Auxin Responsive Fragment Of The Leaco1 Promoter Increases Flower Number In Transgenic Nicotiana Tabacum, Mariya Khodakovskaya, Degang Zhao, William Smith, Yi Li, Richard Mcavoy Jun 2006

Expression Of Ipt Gene Controlled By An Ethylene And Auxin Responsive Fragment Of The Leaco1 Promoter Increases Flower Number In Transgenic Nicotiana Tabacum, Mariya Khodakovskaya, Degang Zhao, William Smith, Yi Li, Richard Mcavoy

Plant Science Articles

Cytokinins play important roles in regulating plant growth and development. A new genetic construct for regulating cytokinin content in plant cells was cloned and tested. The gene coding for isopentenyl transferase (ipt) was placed under the control of a 0.821 kb fragment of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene promoter from Lycopersicon esculentum (LEACO1) and introduced into Nicotiana tabacum (cv. Havana). Some LEACO1(0.821) (kb)-ipt transgenic plant lines displayed normal shoot morphology but with a dramatic increase in the number of flower buds compared to nontransgenic plants. Other transgenic lines produced excessive lateral branch development but no change …


Enhanced Cold Tolerance In Transgenic Tobacco Expressing A Chloroplast Omega-3 Fatty Acid Desaturase Gene Under The Control Of A Cold-Inducible Promoter, Mariya Khodakovskaya, Richard Mcavoy, Jeanne Peters, Hao Wu, Yi Li Apr 2006

Enhanced Cold Tolerance In Transgenic Tobacco Expressing A Chloroplast Omega-3 Fatty Acid Desaturase Gene Under The Control Of A Cold-Inducible Promoter, Mariya Khodakovskaya, Richard Mcavoy, Jeanne Peters, Hao Wu, Yi Li

Plant Science Articles

A new cold-inducible genetic construct was cloned using a chloroplast-specific omega-3-fatty acid desaturase gene (FAD7) under the control of a cold-inducible promoter (cor15a) from Arabidopsis thaliana. RT-PCR confirmed a marked increase in FAD7 expression, in young Nicotiana tabacum (cv. Havana) plants harboring cor15a-FAD7, after a short-term exposure to cold. When young, cold-induced tobacco seedlings were exposed to low-temperature (0.5, 2 or 3.5 degrees C) for up to 44 days, survival within independent cor15a-FAD7 transgenic lines (40.2-96%) was far superior to the wild type (6.7-10.2%). In addition, the major trienoic fatty acid species remained stable …


Fall Fertilization Timing Effects On Nitrate Leaching And Turfgrass Color And Growth, Salvatore Mangiafico, Karl Guillard Jan 2006

Fall Fertilization Timing Effects On Nitrate Leaching And Turfgrass Color And Growth, Salvatore Mangiafico, Karl Guillard

Plant Science Articles

Fall season fertilization is a widely recommended practice for turfgrass. Fertilizer applied in the fall, however, may be subject to substantial leaching losses. A field study was conducted in Connecticut to determine the timing effects of fall fertilization on nitrate N (NO3-N) leaching, turf color, shoot density, and root mass of a 90% Kentucky bluegrass (Poa pratensis L.), 10% creeping red fescue (Festuca rubra L.) lawn. Treatments consisted of the date of fall fertilization: 15 September, 15 October, 15 November, 15 December, or control which received no fall fertilizer. Percolate water was collected weekly with soil monolith lysimeters. Mean log10 …