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Regulation Of The Egfr Epigenetic Landscape In Glioblastoma Multiforme, Anishaa Sivakumar

Honors Undergraduate Theses

Glioblastoma Multiforme (GBM) is the most lethal subtype of glioma (brain tumor), with a 5-year survival rate of merely 5.6% post diagnosis¹. The traditional study of glioblastoma has investigated the role of multiple genes in advancing its progression, including the upregulation of Epidermal Growth Factor Receptor (EGFR). However, there is at yet little research into the epigenetic factors that control EGFR, both in the precursor astrocytes and in glioblastomas themselves². EGFR and its regulation may play a significant role in the progression and development of GBM from astrocytes. Through modification of genomic pathways as observed in …

Genetic Control Of Arbuscular Mycorrhizal Colonization In Helianthus Annuus, Katherine Stahlhut

Honors Undergraduate Theses

Plant symbiosis with arbuscular mycorrhizal (AM) fungi provides many benefits for plants, including increased nutrient uptake, drought tolerance, and belowground pathogen resistance. In order to have a better understanding of the genetic architecture of mycorrhizal symbiosis, we conducted a genome-wide association study (GWAS) by phenotyping a diversity panel of cultivated sunflower (Helianthus annuus) for root colonization under inoculation with the AM fungus Rhizophagus intraradices. This mapping panel consists of 261 inbred lines that capture approximately 90% of the genetic diversity present in the cultivated sunflower germplasm. Using a mixed linear model approach with a high-density genetic map, we …

Investigating The Effects Of Uv Filters In Sunscreen On Human And Environmental Health, Brittany M. Thompson

Honors Undergraduate Theses

Ultraviolet filters are active ingredients in sunscreen that protect us from harmful UV radiation. However, organic UV filters are thought to have adverse effects on the environment and humans. In recent years, fear of harmful impacts of sunscreen has caused a surge of coral reef safe sunscreens to hit the market. These sunscreens, which contain inorganic metal oxides as UV filters, have been accepted as safe for humans and the environment until recently. Metal oxides in reef safe sunscreens may form intermediates in the water that can harm marine life and can absorb through the skin and into the blood, …

Developmental Effects Of Terpenes On Vanessa Cardui At Varying Temperatures, Mari Irving

Honors Undergraduate Theses

Secondary metabolites are organic compounds produced by plants that are not essential in growth, development, or reproduction. While they are not strictly necessary to complete the plant life cycle, secondary metabolites play key roles in functions like defense against herbivory and the moderation of symbiotic relationships. Terpenes are a major class of secondary metabolites present in all plants, and this class is hypothesized to have diversified in response to specific plant-herbivore interactions. These complex interactions are further complicated by changes in abiotic conditions experienced seasonally. Herbivory is a major biotic interaction that plays out across broad temporal and spatial scales …

Validation And Optimization Of Hyperspectral Reflectance Analysis-Based Predictive Models For The Determination Of Plant Functional Traits In Cornus, Rhododendron, And Salix, Milton I. Valdiviezo

Honors Undergraduate Theses

Near infrared spectroscopy (NIR) has become increasingly widespread throughout various fields as an alternative method for efficiently phenotyping crops and plants at rates unparalleled by conventional means. With growing reliability, the convergence of NIR spectroscopy and modern machine learning represent a promising methodology offering unprecedented access to rapid, high throughput phenotyping at negligible costs, representing prospects that excite agronomists and plant physiologists alike. However, as is true of all emergent methodologies, progressive refinement towards optimization exposes potential flaws and raises questions, one of which is the cornerstone of this study. Spectroscopic determination of plant functional traits utilizes plants' morphological and …

The Effects Of Sleep Deprivation On Mental Health And Neurological Disorders, Gabriella Victoria Fernandez De Salvo

Honors Undergraduate Theses

Sleep deprivation is defined as an individual sleeping less than 6 hours per night. It is a common behavior amongst individuals who live in industrialized nations.Along with industrialization, its prevalence is also expected to rise with age. Despite this, there has been a shift in recent years with sleep deprivation increasing in the youth, likely related to the increased use of technology during the nighttime. Sleep deprivation and chronic sleep disruption are behaviors commonly observed amongst patients suffering from neurological and psychiatric disorders, like Alzheimer’s disease, Parkinson’s disease, depression, and anxiety. Many of these patients suffer from sleep disorders like …

The Effect Of A Senolytic Drug On Messenger Rna And Micro Rna Expression In Perigonadal Adipose Tissue Of Young And Old Mice, Catalina Hernandez

Honors Undergraduate Theses

As we face an expanding elderly population and increased age-related chronic diseases, we must seek solutions that address interconnected problems. Dasatinib plus quercetin (D+Q), a novel combination drug, is a proposed solution to improve the health and longevity of aged individuals with minimal side effects, exceptional efficacy, and maximum convenience. In this experiment, intermittent D+Q treatment was completed on young and old mice. RNA was extracted from perigonadal adipose tissue and cDNA libraries of mRNA and miRNA were made. Real-time quantitative polymerase chain reaction was used to assess relative gene expression for mRNA and miRNA pertaining to cell senescence, inflammation, …

The Effects Of Roundup On The Life History, Stress Response, And Immune Function Of The Yellow Fever Mosquito, Aedes Aegypti, Lindsay E. Martin

Honors Undergraduate Theses

Aedes aegypti mosquitoes, vectors for many human diseases, begin life as larvae developing in water, potentially exposed to runoff with herbicides and pesticides. This study serves as a novel investigation into the transstadial effects of exposure to Roundup on A. aegypti life history, immunity, and stress response and aims to account for these effects in an R0 model for vectorborne disease transmission. Prior work has shown that Roundup negatively affects mosquito life history. I hypothesized that larval exposure to the maximum sublethal dose of Roundup (7189µg/L) would negatively impact A. aegypti life history, immunity (candidate gene approach), and stress response …

Professional Liability Insurance For Athletic Trainers: A Cross Sectional Study Of Athletic Trainers Who Do Per Diem Work, Kameelah J. Belgrave

Honors Undergraduate Theses

Context: Many secondary school athletic trainers (ATs) complete per diem work as a way to supplement their income. Working per diem means the AT provides services to events that are not part of their main employment. Since this type of work is not connected to their main employment, the AT may lack appropriate professional liability insurance (PLI). PLI is a type of insurance that protects healthcare professionals from bearing the entire cost of defending a malpractice claim made by a former or current patient. Anecdotally, many ATs believe that the PLI provided by their main employer covers all health care …

Non-Alcoholic Fatty Liver Disease And The Gut Microbiome: The Effects Of Gut Microbial Metabolites On Nafld Progression In A 2-Organ Human-On-A-Chip Model, Rachel H. Boone

Honors Undergraduate Theses

Using a novel, adipose-liver, two-organ, human-on-a-chip system, the metabolic disease non-alcoholic fatty liver disease was modeled. This model was then used to test the effects of the gut microbiome on NAFLD progression. Two products of the gut microbiome, Trimethylamine-n-oxide and butyrate, were selected as representatives of potentially harmful and potentially beneficial compounds. A dose response, adipocyte and hepatocyte monocultures controls, and HoaC systems were run for 14 days. Through this experimentation, it was found that a dysbiosis of the gut microbiome could be influencing NAFLD progression. Additionally, further development and discovery regarding adipose-liver systems was added to the ongoing conversation …

The Effects Of A Plant-Based Diet On Inflammation Of Patients With Cardiac Disease, Rachel L. Butcher

Honors Undergraduate Theses

Cardiac disease is the primary cause of death in the United States of America (CDC, 2017). Despite ongoing efforts and investments to improve cardiac health in the United States, most of the population will suffer from cardiovascular diseases. There is a multitude of research supporting that diet can contribute to cardiac disease, but it is less known that diet can greatly contribute to regulation and reversal of cardiovascular disease processes (Huang et al., 2012; Satija et al., 2017; Kim et al., 2019). Existing research supports the efficacy of plant-based diets to manage and reverse certain cardiac diseases (Tuso et al., …

The Effect Of Using Crispr/Cas9 Treatment To Delete The Myostatin Protein In Vivo And In Vitro, Marco Cardone

Honors Undergraduate Theses

This thesis proposal shows the efficiency of different methods of Myostatin inhibition by using CRISPR/CAS9. With the data cataloged by this thesis, researchers will have a better understanding of what methods are better to achieve their goals. The data was collected by reading multiple scientific articles involving the usage of CRISPR/CAS9 to inhibit the Myostatin protein. The data collected from all the different studies were analyzed in the same categories. The experiment that used CRIPSR/CAS9 on in vitro specimens had a superior Myostatin inhibition overall, therefore presenting higher muscle mass. The method using CRISPR/CAS9 to inhibit the Myostatin in vivo …

Variations In Diet Quality Among Undergraduate Students, William Johnson

Honors Undergraduate Theses

In order to prevent the onset of several chronic diseases, populations that exhibit poor dietary practices must be identified and educated on proper nutritional habits. This study used the pre-validated Rapid Eating Assessment for Participants – Shortened version (REAP-S) survey and a four-question demographic questionnaire to identify these populations among undergraduate students at the University of Central Florida. The participants were 171 students of at least 18 years of age who completed the 17-question online survey. The data collected indicated that significant differences in diet quality regarding consumption of fats and saturated fats exist between ages, class standings, and sexes …

Development Of A Computer Algorithm For Generation Of Primers For Nucleic Acid Sequence Based Amplification (Nasba), Rohit Karnati

Honors Undergraduate Theses

Nucleic acid sequence based amplification (NASBA) is a primer based isothermal method of RNA/DNA amplification. Currently, primer design for NASBA has been restricted to hand creating sequences of oligonucleotides that must follow a set of rules to be compatible for the amplification process. This process of hand-creating primers is prone to error and time intensive. The detection of mutants, post amplification, also offers a benefit in point of care scenarios and the design of hybridization probes for sequences in the region of amplification is also an erroneous and time intensive process. By creating a program to design primers and hybridization …

Investigating The Effect Of Ethanol On Wnt7a And Its Potential Implications In Fetal Alcohol Spectrum Disorder, Erika Lytle

Honors Undergraduate Theses

Fetal Alcohol Spectrum Disorders (FASDs), are caused by maternal alcohol consumption during pregnancy [3]. FASD encompasses a wide variety of cardiac and neural anomalies, while also associated with improper limb development, abnormal craniofacial features, problems within the central nervous system (CNS), and disabilities in learning and communication. Gene-regulating FASDs have not been well studied during the crucial phases of early embryonic development. Genes within the Wnt/Beta-catenin pathway control a vast amount of embryonic developmental processes. Among them is the Wnt7a gene, a significant downstream gene regulator which positively controls neural stem cell proliferation and cardiomyocyte differentiation on a large scale …

Exploring The Impact Of Pre-Exposure Prophylaxis Related To Sexual Behavior In College Men, Dalton J. Poe

Honors Undergraduate Theses

Pre-exposure prophylaxis (PrEP) is an effective tool for preventing human immunodeficiency virus (HIV) acquisition in sexually active at-risk individuals such as men who have sex with men (MSM). The purpose of this study is to examine factors associated with intent to engage in risky sexual behavior among HIV-negative college aged (18-24) MSM who are currently adherent to PrEP or who have expressed interest in the future adoption of PrEP. A multiracial/ethnic sample of 31 men expressing interest in the adoption of PrEP and 6 men currently taking PrEP completed a quantitative survey identifying key themes regarding attitudes towards PrEP and …

Genetic Structure Of Green Sea Turtle (Chelonia Mydas) Foraging Aggregations On The East Coast Of Florida, Monica R. Reusche

Honors Undergraduate Theses

The genetic structure of juvenile green turtles (Chelonia mydas) foraging on the east coast of central Florida is not well understood, nor has it been examined over time. In the last three decades, the dramatic increase in the number of green sea turtle nests in Florida, in association with other population parameters, has led to this species being down-listed under the Endangered Species Act from “endangered” to “threatened” in the northwest Atlantic. However, it was unclear if the exponential growth in Florida nest numbers had any influence on the genetic structure of juveniles in nearby foraging aggregations. To …

Characterization Of The Physical And Chemical Effect Of Membrane Disruption And Protein Inhibiting Treatments On E. Coli, Khadijah Wright

Honors Undergraduate Theses

The increase in antibacterial resistance has placed the issue of microbial multi-drug resistance on a global stage (Gurunathan, 2019). This issue poses a threat to human and animal health as well as to the environment (Aslam et al., 2018). It affects not only the efficacy of treatment but also how those treatments are conducted (Friedman, Temkin, & Carmeli, 2016). As a result of this ongoing threat, new treatments that have potent effects on bacteria are necessary. One scientific response to this issue has been the development of multifunctional nanoparticles (NPs)(H. Wang et al., 2018). NPs have the ability to be …

Role Of Polyploidy In Leaf Functional Trait Evolution Across Wild Helianthus, Anestacia S. Robinson

Honors Undergraduate Theses

Whole genome duplication, or polyploidy, is a common process in plants by which failures in meiosis or fertilization result in offspring with twice the number of chromosomes. This doubles the number of copies of every gene, an effect thought to generate new ‘raw material' upon which natural selection can act. Few studies exist examining the consequences of polyploidy for plant physiological traits. Doubling the number of gene copies may have unknown effects on leaf structure and function. In this study, I compare diploid, tetraploid, and hexaploid species within the genus Helianthus (wild sunflowers). Forty different accessions of wild sunflowers were …

Evaluation Of Remote-Based Learning Of A Nutrition Program For Adolescents With Autism Spectrum Disorder: A Covid-19 Pilot Study, Riley H. Shurack

Honors Undergraduate Theses

Introduction: Adolescents with Autism Spectrum Disorder (ASD) demonstrate poor health-related behaviors, such as nutrition, compared to their typically developed (TD) counterparts. With the occurrence of the COVID-19 pandemic and mandatory stay-at-home orders, such health-related behaviors may be complicated and there is a need to focus on feasibility and acceptability of in-person and remote-based nutrition programs.

Methods: Eleven high school students with ASD were recruited to participate in a modified nutrition program utilizing Zoom software during COVID-19. The duration was once a week for four weeks, and concepts reviewed included shopping for healthy food on a budget, introducing non-perishable …

Evaluating The Nutritional Status Of Peruvian Born Children, Chantelle Garcia Medina

Honors Undergraduate Theses

Background: Rural areas of Peru lack access to healthcare resources and poor nutritional knowledge of what should be given to their children throughout their growth and development. The majority of the rural areas rely on a high carbohydrate, moderate vegetable diet, and lacking a protein source. Lower protein intake can lead to problems of malnutrition and growth stunting. Families rarely have monetary resources to provide a protein at every meal for the entire family. There is also a lack of a clean water supply, free of parasites and helminths. Caregivers often rely on replenishing intake with other fluids high …

Affective Response To Upper Body And Lower Body Exercise, Shanelle J. Osorio

Honors Undergraduate Theses

More than one-half of university students in the United States and Canada are not active enough to gain health benefits. Enjoyment of exercise proposes a feasible solution to the absence of motivation surrounding physical activity. The purpose of this study is to compare the differences in reported enjoyment between upper and lower body cycling graded exercise to exhaustion (GXT). Seven university students (23 ± 3 years old; 26 ± 4 kg/m2) performed two randomized graded exercise tests on different days: one for upper body, one for lower body. Feeling Scale (FS) measured the affective response during exercise. Post-exercise enjoyment values …

Sensing Symbiosis: Investigating The Symbiotic Magnetic Sensing Hypothesis In Fish Using Genomics, Elizabeth Boggs

Honors Undergraduate Theses

The mechanism behind magnetoreception – the ability to sense magnetic fields for orientation and navigation – still remains one of the most difficult questions to answer in sensory biology, with fish being just one of many taxa known to possess this sense. Characterizing a magnetic sense in fish is crucial for understanding how they navigate their environment and can inform on how increasing anthropogenic sources of electromagnetic fields in aquatic environments may affect threatened fish species. This study examined the hypothesis put forth by Natan and Vortman (2017) that magnetotactic bacteria (MTB), bacteria that create their own chains of magnetic …