Digital Commons Network^™

Investigating Plant Physiological Responses To Global Phylogenetic Diversity Of Glomeromycotina, David Z. Mowbray

Honors Undergraduate Theses

Arbuscular mycorrhizal (AM) fungi are ubiquitous symbionts of terrestrial plant species with associations predominantly characterized as mutualistic. In addition to well-documented enhancement of host growth response, more recent analyses have demonstrated the conferral of host benefits under numerous biotic and abiotic stressors. However, much of the established evidence originates from studies involving limited AM fungal diversity. Accordingly, this study sought to evaluate the potential effects of inoculation on plant host physiological traits within a growth chamber environment, investigate potential correlations between host trait responses, & assess the degree of phylogenetic signal observed in trait responses due to the presence of …

Microplastic Abundances In The Guana River Estuary In Northeast Florida, Mckenna Keplinger

Honors Undergraduate Theses

Plastic never fully disappears, but instead breaks into smaller pieces referred to as microplastics (< 5 mm length). Microplastics are common worldwide, and more studies are needed to understand the accumulation and diversity of microplastics found in various environments. In this study, six locations were sampled for one year in the Guana River Estuary, a partially impounded system with heavily urbanized headwaters. This study was conducted in conjunction with the Guana Tolomato Matanzas National Estuarine Research Reserve (GTM NERR) and the Florida Fish and Wildlife Conservation Commission (FWC) at their water sampling stations. The objectives of this study were to investigate: 1) distribution patterns across sample sites over time, 2) the color, size, and shape of microplastics found, and 3) polymer composition. Water samples were collected from surface waters in 1-L bottles, with five replicates at each site. Sampling occurred once a month, at the beginning of each month from August 2020-August 2021. Samples were analyzed for abundance and characteristics of microplastics using a dissecting microscope and the polymer composition was determined using Fourier-transform infrared spectroscopy. No pattern was found in the distribution of microplastics from north to south or over time. Variations in plastic color, size, and polymer composition suggests that there are multiple sources of pollution into the Guana River Estuary.

Investigating The Phytochemical Profiles Of Wild And Cultivated Blueberries (Vaccinium Spp.) In Central Florida, Anisa Khalid

Honors Undergraduate Theses

Blueberries (Vaccinium spp.) are valued for their high nutritional quality and flavor. There are five species in the Vaccinium genus native to the state of Florida, but only three are palatable to humans: V. myrsinites, V. darrowii, and V. corymbosum. While V. darrowii has been studied extensively due to its successful hybridization with northern highbush blueberry cultivars, V. myrsinites and wild V. corymbosum have been understudied. This study aims to 1) quantify the abundance of various phytochemicals in fruits of these wild blueberry species and compare fruit quality both among the wild taxa and two varieties of …

Bioprospecting For Improved Floral Fragrance In Wild Sunflower, Jason Anandappa

Honors Undergraduate Theses

The cultivation of crop sunflower or Helianthus annuus is a 20 billion USD industry globally. Crop wild relatives of H. annuus have a long history of being used to breed improved traits into cultivated varieties. Cultivated H. annuus is not known to have a pleasant aroma, and at times seed yield is limited by pollination services, particularly in the production of hybrid seed. Improved floral fragrance could improve pollinator attraction and would add value to ornamental sunflowers in the context of the cut-flower industry. If volatile organic compounds that together generate favorable scents are present in wild Helianthus species, they …

Phototransduction Components In The Visual System Of Hard-Bodied Ticks, Arimar J. López Limas

Honors Undergraduate Theses

Ticks are terrestrial invertebrate parasites that attach to their hosts to feed on their blood. Ticks are composed of three families: Ixodidae, Argasidae, and Nuttalliellidae. Ixodid ticks include members of the genera Amblyomma, Ixodes, Haemaphysalis, Hyalomma, Dermacentor, and Rhipicephalus. Ticks can transmit diseases to animals and humans, making them an important organism to study. Current tick-bite mitigation strategies include acaricides (harmful if misused), and CO₂ traps (effective in decreasing tick abundance, but costly to use). Since the visual system of ticks has not been studied extensively, I expect that by studying their visual system, alternative tick-bite mitigation …

The Implications Of Case-Based Learning (Cbl) On Biochemistry Education: An Integrative Literature Review, Ryan C. Ripert

Honors Undergraduate Theses

The COVID-19 has significantly impacted the world as we know it, especially in higher education institutions. In response to COVID-19, most colleges and universities were forced to close their doors to students and switch to online learning over Zoom. Although most students are adjusted well to this new mode of education, other students, such as individuals pursuing degrees in the sciences, have difficulty adapting to the online mode of instruction. Case-based learning (CBL) is considered one of the best strategies in teaching a course like Biochemistry in a large classroom setting. Implementing CBL in biochemistry education may increase student engagement …

Linking Magnetoreception To Cacna1d Homolog Protein Insertions In Vertebrates, Annabelle S. Levin

Honors Undergraduate Theses

It is well-established that numerous animals can perceive the geomagnetic field. This ability, called magnetoreception, has been studied extensively but the mechanisms for how animals perceive the geomagnetic field remain unclear. Understanding magnetoreception is vital for characterizing the navigational abilities of animals and how biological systems interact with magnetic fields. Recent research examining pigeons, an animal is known to exhibit a magnetic sense, identified two interesting genes that may be involved in magnetoreception. One of these genes, calcium voltage-gated channel subunit alpha1 D (CACNA1D), contains a short amino acid insertion (designated by the amino acids -KKER) in animals known to …

Consequences Of Stress-Induced Trait Plasticity In Cultivated Helianthus, Gillian Gomer

Honors Undergraduate Theses

The Leaf Economics Spectrum (LES) describes the continuous range of leaf ecophysiological strategies that plants across the globe utilize to achieve a net-positive return on their resource investment. This spectrum is measured by traits such as leaf mass per area, leaf nitrogen, and leaf lifespan. Unsuitable leaf trait combinations are selected against in nature due to a net-loss on the return of initial resource investment, while some potentially extremely effective trait combinations may not be possible due to genetic constraints. Existing genetic variation in LES traits, however, suggests the spectrum may be less rigid than previously expected. To test this, …

Stable Isotope Analysis Of An Invasive Crab Species, Charybdis Hellerii, In The Indian River Lagoon, Justin R. Meyer

Honors Undergraduate Theses

The world has never been more connected than it is today. While this is true for people, it is also true for Earth's flora and fauna. Unfortunately, this connectedness has contributed to unprecedented invasive species introductions around the world. Most introductions result in an introduced species dying out in the newly invaded territory and never becoming established. Other introduced species establish and persist for years, but never have a noticeable effect on local ecosystems. However, occasionally, an invasive species gets introduced to a new area and has negative impacts on native plant and animal life. The Indo-Pacific swimming crab, Charybdis …

Genetic Control Of Arbuscular Mycorrhizal Colonization In Helianthus Annuus, Katherine Stahlhut

Honors Undergraduate Theses

Plant symbiosis with arbuscular mycorrhizal (AM) fungi provides many benefits for plants, including increased nutrient uptake, drought tolerance, and belowground pathogen resistance. In order to have a better understanding of the genetic architecture of mycorrhizal symbiosis, we conducted a genome-wide association study (GWAS) by phenotyping a diversity panel of cultivated sunflower (Helianthus annuus) for root colonization under inoculation with the AM fungus Rhizophagus intraradices. This mapping panel consists of 261 inbred lines that capture approximately 90% of the genetic diversity present in the cultivated sunflower germplasm. Using a mixed linear model approach with a high-density genetic map, we …

Investigating The Effects Of Uv Filters In Sunscreen On Human And Environmental Health, Brittany M. Thompson

Honors Undergraduate Theses

Ultraviolet filters are active ingredients in sunscreen that protect us from harmful UV radiation. However, organic UV filters are thought to have adverse effects on the environment and humans. In recent years, fear of harmful impacts of sunscreen has caused a surge of coral reef safe sunscreens to hit the market. These sunscreens, which contain inorganic metal oxides as UV filters, have been accepted as safe for humans and the environment until recently. Metal oxides in reef safe sunscreens may form intermediates in the water that can harm marine life and can absorb through the skin and into the blood, …

Developmental Effects Of Terpenes On Vanessa Cardui At Varying Temperatures, Mari Irving

Honors Undergraduate Theses

Secondary metabolites are organic compounds produced by plants that are not essential in growth, development, or reproduction. While they are not strictly necessary to complete the plant life cycle, secondary metabolites play key roles in functions like defense against herbivory and the moderation of symbiotic relationships. Terpenes are a major class of secondary metabolites present in all plants, and this class is hypothesized to have diversified in response to specific plant-herbivore interactions. These complex interactions are further complicated by changes in abiotic conditions experienced seasonally. Herbivory is a major biotic interaction that plays out across broad temporal and spatial scales …

Validation And Optimization Of Hyperspectral Reflectance Analysis-Based Predictive Models For The Determination Of Plant Functional Traits In Cornus, Rhododendron, And Salix, Milton I. Valdiviezo

Honors Undergraduate Theses

Near infrared spectroscopy (NIR) has become increasingly widespread throughout various fields as an alternative method for efficiently phenotyping crops and plants at rates unparalleled by conventional means. With growing reliability, the convergence of NIR spectroscopy and modern machine learning represent a promising methodology offering unprecedented access to rapid, high throughput phenotyping at negligible costs, representing prospects that excite agronomists and plant physiologists alike. However, as is true of all emergent methodologies, progressive refinement towards optimization exposes potential flaws and raises questions, one of which is the cornerstone of this study. Spectroscopic determination of plant functional traits utilizes plants' morphological and …

The Effects Of Sleep Deprivation On Mental Health And Neurological Disorders, Gabriella Victoria Fernandez De Salvo

Honors Undergraduate Theses

Sleep deprivation is defined as an individual sleeping less than 6 hours per night. It is a common behavior amongst individuals who live in industrialized nations.Along with industrialization, its prevalence is also expected to rise with age. Despite this, there has been a shift in recent years with sleep deprivation increasing in the youth, likely related to the increased use of technology during the nighttime. Sleep deprivation and chronic sleep disruption are behaviors commonly observed amongst patients suffering from neurological and psychiatric disorders, like Alzheimer’s disease, Parkinson’s disease, depression, and anxiety. Many of these patients suffer from sleep disorders like …

Role Of Polyploidy In Leaf Functional Trait Evolution Across Wild Helianthus, Anestacia S. Robinson

Honors Undergraduate Theses

Whole genome duplication, or polyploidy, is a common process in plants by which failures in meiosis or fertilization result in offspring with twice the number of chromosomes. This doubles the number of copies of every gene, an effect thought to generate new ‘raw material' upon which natural selection can act. Few studies exist examining the consequences of polyploidy for plant physiological traits. Doubling the number of gene copies may have unknown effects on leaf structure and function. In this study, I compare diploid, tetraploid, and hexaploid species within the genus Helianthus (wild sunflowers). Forty different accessions of wild sunflowers were …

Sensing Symbiosis: Investigating The Symbiotic Magnetic Sensing Hypothesis In Fish Using Genomics, Elizabeth Boggs

Honors Undergraduate Theses

The mechanism behind magnetoreception – the ability to sense magnetic fields for orientation and navigation – still remains one of the most difficult questions to answer in sensory biology, with fish being just one of many taxa known to possess this sense. Characterizing a magnetic sense in fish is crucial for understanding how they navigate their environment and can inform on how increasing anthropogenic sources of electromagnetic fields in aquatic environments may affect threatened fish species. This study examined the hypothesis put forth by Natan and Vortman (2017) that magnetotactic bacteria (MTB), bacteria that create their own chains of magnetic …

The Hyperaccumulation Of Zinc In Sunflowers And Its Effect On Disease Resistance, Rayner J. Seavey

Honors Undergraduate Theses

Sunflowers are considered to be a part of a group of plants known as hyperaccumulators that share the ability to accumulate high amounts of heavy metals in the above ground organs, far in excess of the levels found in other species, often without suffering any phytotoxic effects. Quantifying the effects of zinc accumulation through the lens of the elemental defense hypothesis is essential for uncovering if there is a means to increase herbivore resistance in agricultural settings without the use of external interventions such as pesticides. A greenhouse study was conducted on four widely grown commercial cultivars of sunflower. Each …

Investigation Of Microplastic Accumulation In The Gastrointestinal Tract In Birds Of Prey, Julia Carlin

Honors Undergraduate Theses

Plastic pollution is unavoidable in the natural environment. Consequences of plastic ingestion include exposure to environmental pollutants and toxin accumulation, causing endocrine disruption, inflammatory and physiological stress in organisms. Microplastics have been shown to transfer across food webs, however, limited studies have examined microplastic accumulation across terrestrial food webs. Furthermore, few studies have examined plastic pollution in apex predatory animals. A study was conducted to quantify the abundance of plastic pollution in the gastrointestinal tract in birds of prey. Two species were investigated, one which forages in terrestrial habitats and one which forages in aquatic environments including Buteo lineatus (red-shouldered …

Assessing The Impact Of Oyster Reef And Living Shoreline Restoration On Macroinvertebrate Community Assemblages In Mosquito Lagoon, Florida, Adam Searles

Honors Undergraduate Theses

As the world continues to experience substantial rates of habitat loss, habitat restoration has become of prime interest to ecologists worldwide. Restoration has shown to be successful in recovering targeted components of certain ecosystems but it is important to achieve a holistic understanding of the resulting ecological impacts it has on communities. To address this, four oyster reefs and three living shorelines were restored during the summer of 2017. These sites, along with four dead oyster reefs, four living oyster reefs, and three undisturbed (control) living shorelines, were sampled before restoration and regularly post-restoration for one year using lift nets. …

Micromanipulation And Genetic Analysis Of Individual Sperm Cells For Sexual Assault Investigations, Amanda Penn

Honors Undergraduate Theses

Sexual assault investigations utilize both physical and biological evidence to aid in the investigation. Physical evidence may include fingerprints, hair, fibers, stains, soil, and glass. Biological evidence may include semen, saliva, vaginal secretions, menstrual blood, and skin. Semen, often found in small or trace quantities, is of great importance when trying to identify the perpetrator. From the semen sample, DNA profiles using autosomal short tandem repeats (aSTRs) (gold standard in forensic science) or Y-chromosome short tandem repeats (Y-STRs) can be obtained and can be used to identify a perpetrator through comparison to suspect reference samples or by searching the profile …

Investigating The Role Of Neuronal Aging In Fragile X-Associated Tremor/Ataxia Syndrome, Katlin Marie Hencak

Honors Undergraduate Theses

Fragile X-associated tremor/ataxia syndrome (FXTAS) is an X-linked late-onset neurodegenerative disorder caused by a noncoding trinucleotide repeat expansion in the FMR1 gene. This gene produces fragile x mental retardation protein (FMRP), an RNA binding protein whose targets are involved in brain development and synaptic plasticity. One of the proposed mechanisms of FXTAS pathogenesis is an RNA gain-of-function in which the repeat expansion causes toxic mRNA that sequesters important proteins in the cell, interfering with their functions. Another suggested method of pathogenesis is through a mutant protein called FMRpolyG. This protein results from repeat-associated non-AUG (RAN) translation, in which the expanded …

Capillary Electrophoresis Buffer Optimization For Plant Tissue Analysis, Rebekah Davis

Honors Undergraduate Theses

Capillary electrophoresis (CE) is an analytical chemistry approach that allows for the efficient separation by charge of diverse classes of compounds for analysis, including secondary metabolites. The goal of this work was to optimize a buffer system for plant tissue analysis using micellar electrokinetic chromatography (MEKC), and by doing so to understand the role of buffer components in the performance of this form of capillary electrophoresis. In this experiment we implemented a factorial design to optimize buffer composition for separating plant tissue and secondary metabolites. The results of this experiment will be used to optimize a universal buffer for MEKC …

Elucidating The Role Of Oxygen And Biotype In The Environmental Persistence Of Vibrio Cholerae, Amy M. Freiberg

Honors Undergraduate Theses

Vibrio cholerae is a natural inhabitant of aquatic environments and serves as the etiological agent for the severe diarrheal disease, cholera. Cholera epidemics follow a regular seasonal pattern, which account for tens to hundreds of thousands of deaths in a given year. V. choleraenaturally persist between epidemics through entry into a dormant state known as viable but nonculturable (VBNC). Research has shown that V. choleraein this VBNC state experience drastic morphological and metabolic changes, which serve as survival mechanisms until environmental conditions become suitable again. The natural marine ecosystem that V. cholerae inhabitis comprised of a complex …

Oyster Reef Restoration: Impacts On Infaunal Communities In A Shallow Water Estuary, Katherine P. Harris

Honors Undergraduate Theses

Oyster reefs are important estuarine ecosystems that provide habitat to many species including threatened and endangered wading birds and commercially important fishes and crabs. Infaunal organisms (i.e. small, aquatic animals that burrow in the sediment) are also supported by oyster reef habitats. Infaunal organisms are critical to marine food webs and are consumed by many important species that inhabit coastal estuaries. However, over the past century 85% of shellfish reef habitats have been lost, making restoration of these areas vital. Due to their important role in coastal food webs, infauna is hypothesized to be a strong indicator of habitat productivity …

Mechanical Properties Of The Skeleton Of Acropora Cervicornis, Bridget Masa

Honors Undergraduate Theses

This research explores the instantaneous mechanical behavior of the skeleton of the critically endangered staghorn coral Acropora cervicornis. Both bleached and sanded skeletons were used in this experiment. The Raman spectroscopy test showed that there was no significant change in the Raman shift between the three branches tested. The shifts were nearly identical to Raman shifts of calcium carbonate. Vickers hardness test found that 1 Bleached had the average hardness of 3.44 GPa with a standard deviation of 0.12 GPa. The sanded sample also had a similar value of 3.54 GPa with a standard deviation of 0.13 GPa. Samples from …

Infection Dynamics Of Herpesvirus In Gopher Tortoises, Joanne Saldanha

Honors Undergraduate Theses

Gopherus polyphemus, commonly known as the Gopher Tortoise, is a dryland reptile native to the southeastern United States. It is commonly a resident of longleaf pine and dry oak sand hill habitats. It is considered a keystone species because they dig deep burrows that provide shelter to them as well as many other animals. Habitat loss, fragmentation, and disease are major threats and have caused this species to be federally listed as a threatened species under the Endangered Species Act (ESA). Disease is a major threat to the gopher tortoise’s survival, and with declining populations, the need to investigate …

Split Deoxyribozyme Probe For Efficient Detection Of Highly Structured Rna Targets, Sheila Raquel Solarez

Honors Undergraduate Theses

Transfer RNAs (tRNAs) are known for their role as adaptors during translation of the genetic information and as regulators for gene expression; uncharged tRNAs regulate global gene expression in response to changes in amino acid pools in the cell. Aminoacylated tRNAs play a role in non-ribosomal peptide bond formation, post-translational protein labeling, modification of phospholipids in the cell membrane, and antibiotic biosynthesis. ^[1] tRNAs have a highly stable structure that can present a challenge for their detection using conventional techniques. ^[2] To enable signal amplification and lower detection limits, a split probe - split deoxyribozyme (sDz or BiDz) probe, which …

An Rnai Screen To Identify Components Of A Polyamine Transport System, Adam J. Foley

Honors Undergraduate Theses

Polyamines, specifically putrescine, spermidine, and spermine, are small cationic molecules found in all organisms. Cells can biosynthetically make these molecules, or alternatively, they can be transported from the extracellular environment. Malignant cells have been shown to require relatively high amounts of polyamines. There is a chemotherapeutic agent, DFMO, used to block the biosynthesis of polyamines. Many malignant cells can circumvent DFMO therapy by activating their transport system. A potential solution is to simultaneously block biosynthesis and transport of polyamines. However, little is known about the polyamine transport system in higher eukaryotes.

This thesis aims to add to the basic biological …

The Effect Of Climate On Physiology And Immune Function In The Asian Citrus Psyllid, Diaphorina Citri, Grace Avecilla

Honors Undergraduate Theses

The variation in the insect immune system is an important regulator of insect populations and the pathogens they carry. A central component of insect immunity is melanin, whose production creates cytotoxic intermediates that help to protect against a broad spectrum of pathogens. Melanin is also used in insect cuticle where it helps to improve thermoregulation and desiccation resistance, with insects having less melanized cuticles in warmer and more humid environments. Considering that cuticle melanin and immune melanin are formed by near identical biochemical pathways, they are pleiotropically linked (that is, one or more linked genes influence multiple traits). This has …