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A Tripartile Biosensor For Real-Time Snss Detection In Dna Hairpin Motif, Camha Nguyen
A Tripartile Biosensor For Real-Time Snss Detection In Dna Hairpin Motif, Camha Nguyen
HIM 1990-2015
The hybridization between two complementary strands of nucleic acid is the basis for a number of applications in DNA and RNA analysis, including in vivo RNA monitoring, microarrays, SNPs detection, and so on. The short oligonucleotide probes form Watson/Crick base pairs (A-T and G-C) with the analyzed nucleic acid. Molecular beacon (MB) probe is one of the most advantageous tools for nucleic acid analysis in real-time. A traditional MB probe consists of a DNA strand folded in hairpin motif with a fluorophore attached to the 5'end and a quencher attached to the 3' end. The loop segment is complementary to …