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The Role Of Atpase In The Development Of Idiopathic Pulmonary Arterial Hypertension In Broilers, Jessica Worley
The Role Of Atpase In The Development Of Idiopathic Pulmonary Arterial Hypertension In Broilers, Jessica Worley
Biological Sciences Undergraduate Honors Theses
The goal of this study was to assess the ATPase activity in the vascular walls of the pulmonary artery, 1A arteriole, and 2A arteriole of the lung of broilers susceptible to idiopathic pulmonary arterial hypertension. This was achieved through the modification of tryptophan in the diet and through the examination of the ATPase activity in the different pulmonary vessels. A QuantiChrom ATPase assay quantified the amount of phosphate ion correlating to the amount of ATPase activity, which was normalized for vessel size using the Coomassie and MicroBCA Protein Assays (giving ?M of phosphate/?g of protein per mL). One way repeated …