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Saccharomyces Cerevisiae Sec59 Cells Are Deficient In Dolichol Kinase Activity, Loree Heller, Peter Orlean, W. Lee Adair Jr.
Saccharomyces Cerevisiae Sec59 Cells Are Deficient In Dolichol Kinase Activity, Loree Heller, Peter Orlean, W. Lee Adair Jr.
Bioelectrics Publications
The temperature-sensitive Saccharomyces cerevisiae mutant sec59 accumulates inactive and incompletely glycosylated protein precursors in its endoplasmic reticulum at the restrictive temperature. O-mannosylation and glycosyl phosphatidylinositol membrane anchoring of protein are also abolished, consistent with a deficiency in dolichyl phosphate mannose. Membranes prepared from sec59 cells that had been shifted to the restrictive temperature, however, made normal amounts of dolichyl phosphate mannose when exogenous dolichyl phosphate was supplied, but dolichyl phosphate mannose synthesis was severely depressed in the absence of exogenous dolichyl phosphate. Quantitative measurements of dolichyl phosphate in sec59 cells showed that the levels were decreased to 48% of wild …
Temperature And Suction Effects On The Instability Of An Infinite Swept Attachment Line, D. G. Lasseigne, T. L. Jackson, F. Q. Hu
Temperature And Suction Effects On The Instability Of An Infinite Swept Attachment Line, D. G. Lasseigne, T. L. Jackson, F. Q. Hu
Mathematics & Statistics Faculty Publications
It is known that the incompressible, infinite swept attachment line flow is unstable to streamwise disturbances that originate in the boundary layer when the cross-flow exceeds a critical magnitude. Furthermore, a small degree of suction at the surface has a significant stabilizing influence while a small degree of blowing has a considerable destabilizing influence. This paper investigates the stabilizing and destabilizing effects of, respectively, cooling or heating the plate and the competing or enhancing effects of suction or blowing. A nonorthogonal flow with respect to the attachment line is also considered by adding a component of shear to the mean …