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Identification Of Regulatory Roles For Stress Responsive Transposable Elements In Rice, Jenna M. Handal, Paul Lup, Emalynn E. Tobias, Andy Pereira, Navdeep Gill
Identification Of Regulatory Roles For Stress Responsive Transposable Elements In Rice, Jenna M. Handal, Paul Lup, Emalynn E. Tobias, Andy Pereira, Navdeep Gill
Bioinformatics
Transposable elements, or mobile DNA sequences, are known for their structural and functional roles in the rice genome. As sources of genetic variation and drivers of evolution, transposable elements have been extensively studied for their role in regulation of gene expression. In addition, transposable elements are shown to be activated in response to various biotic and abiotic stresses and can possibly serve as a contributor to heat stress adaptation by modification of gene expression. As the global temperature rises, heat stress threatens crop production worldwide by affecting optimal rice growth, development, and yield. Concern for food security amidst a growing …
Identification And Cloning Of Putative Serine Protease Inhibitor (Serpin) Genes In Rice (Oryza Sativa) And A Preliminary Approach To Generate Rnai Using The Cloned Sequences, Santanu De
Biology Faculty Articles
Programmed Cell Death (PCD) is an important mechanism of plant immune response against diseases. Serine protease inhibitors (‘serpins’) are a conserved superfamily of proteins that inhibit serine protease targets and prevent programmed cell death (PCD) in plants, in absence of pathogen infection. In this project, putative serpin genes in rice (Oryza sativa) which are homologous to necrotic/‘Nec’ serpin genes in Drosophila are identified by bioinformatic analyses. This is followed by cloning of specific exon sequences of the rice serpin genes identified. Finally, one of the cloned sequences is utilized in a series of steps to produce interfering RNA …
Functional And Structural Divergence Of An Unusual Ltr Retrotransposon Family In Plants, Dongying Gao, Jose Jimenez-Lopez, Aiko Iwata, Navdeep Gill, Scott Jackson
Functional And Structural Divergence Of An Unusual Ltr Retrotransposon Family In Plants, Dongying Gao, Jose Jimenez-Lopez, Aiko Iwata, Navdeep Gill, Scott Jackson
Biology Faculty Articles
Retrotransposons with long terminal repeats (LTRs) more than 3 kb are not frequent in most eukaryotic genomes. Rice LTR retrotransposon, Retrosat2, has LTRs greater than 3.2 kb and two open reading frames (ORF): ORF1 encodes enzymes for retrotransposition whereas no function can be assigned to ORF0 as it is not found in any other organism. A variety of experimental and in silico approaches were used to determine the origin of Retrosat2 and putative function of ORF0. Our data show that not only is Retrosat2 highly abundant in the Oryza genus, it may yet be active in rice. Homologs of Retrosat2 …